Weak gamma-glutamyl:cysteine ligase; ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. However, because of the low catalytic rate, the question remains whether L-cysteine is the actual biological substrate; Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.

DUF419 family protein.

Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive; Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4- dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species; Belongs to the nitroreductase family.

2'-5' RNA ligase; Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'- phosphomonoester. In vitro, can also ligate 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. This reaction does not require ATP and is reversible. Belongs to the 2H phosphoesterase superfamily. ThpR family.

Sugar fermentation stimulation protein A; Binds to DNA non-specifically. Could be a regulatory factor involved in maltose metabolism.

glutamyl-Q tRNA(Asp) synthetase; Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in position 34 of the tRNA(Asp), the wobble position of the QUC anticodon. Does not transfer glutamate to either tRNA(Glu) or tRNA(Gln). The incapacity of the glutamylated tRNA(Asp) to bind elongation factor Tu suggests that it is not involved in ribosomal protein biosynthesis.

Putative colanic acid biosynthesis protein; Protein involved in colanic acid biosynthetic process.