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| | dnaA | Chromosomal replication initiator protein DnaA; Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'- TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. Belongs to the DnaA family. (445 aa) |
| | gyrB | DNA gyrase, B subunit; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (642 aa) |
| | hisS | histidyl-tRNA synthetase; Identified by match to protein family HMM TIGR00442. (418 aa) |
| | dinB | DNA polymerase IV, putative; Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII. (390 aa) |
| | dtd | D-tyrosyl-tRNA(Tyr) deacylase; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. Belongs to the DTD family. (153 aa) |
| | DET0047 | DNA polymerase III delta subunit-related protein; Identified by similarity to SP:P28630. (399 aa) |
| | alaS | alanyl-tRNA synthetase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain. (871 aa) |
| | DET0051 | Conserved hypothetical protein TIGR00250; Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA; Belongs to the YqgF nuclease family. (133 aa) |
| | tgt | Queuine tRNA-ribosyltransferase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form th [...] (392 aa) |
| | radA | DNA repair protein RadA; DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function. (462 aa) |
| | cysS | cysteinyl-tRNA synthetase; Identified by match to protein family HMM TIGR00435; Belongs to the class-I aminoacyl-tRNA synthetase family. (458 aa) |
| | DET0063 | Resolvase domain protein. (542 aa) |
| | DET0066 | DNA methylase; Identified by match to protein family HMM PF01555; match to protein family HMM PF02195; Belongs to the N(4)/N(6)-methyltransferase family. (411 aa) |
| | DET0069 | HNH endonuclease domain protein. (118 aa) |
| | DET0074 | DNA-dependent DNA polymerase, family A; Identified by similarity to SP:P06225. (646 aa) |
| | DET0076 | Resolvase domain protein. (205 aa) |
| | DET0083 | ISDet1, transposase orfA; Identified by similarity to GP:2645860. (88 aa) |
| | DET0084 | Conserved domain protein; Identified by similarity to SP:P21891. (96 aa) |
| | DET0108 | Identified by match to protein family HMM PF03372. (591 aa) |
| | DET0155 | Resolvase domain protein; Identified by match to protein family HMM PF00239; match to protein family HMM PF07508. (510 aa) |
| | DET0157 | Site-specific recombinase, phage integrase family; Identified by match to protein family HMM PF00589. (234 aa) |
| | DET0160 | DNA polymerase domain protein. (425 aa) |
| | DET0165 | ISDet2, transposase orfA; Identified by similarity to SP:P24580. (88 aa) |
| | DET0166 | ISDet2, transposase orfB; Identified by similarity to GP:2645861; match to protein family HMM PF00665. (274 aa) |
| | DET0169 | RNA polymerase sigma factor SigW, putative; Identified by similarity to SP:Q45585; Belongs to the sigma-70 factor family. ECF subfamily. (183 aa) |
| | DET0180 | Reductive dehalogenase, putative; Identified by similarity to GP:3002555; similarity to GP:5531941; match to protein family HMM TIGR01409. (455 aa) |
| | DET0189 | Methyltransferase, putative; Identified by match to protein family HMM TIGR00095. (192 aa) |
| | leuS | leucyl-tRNA synthetase; Identified by similarity to SP:P36430; Belongs to the class-I aminoacyl-tRNA synthetase family. (813 aa) |
| | nrd-1 | Ribonucleotide reductase; Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen. (585 aa) |
| | DET0251 | Transcriptional repressor LexA, putative; Identified by similarity to SP:P31080; Belongs to the peptidase S24 family. (217 aa) |
| | DET0253 | DNA primase domain protein; Identified by similarity to SP:Q08346. (868 aa) |
| | DET0254 | Resolvase domain protein. (211 aa) |
| | DET0272 | Site-specific recombinase, phage integrase family; Belongs to the 'phage' integrase family. (319 aa) |
| | DET0323 | Site-specific recombinase, phage integrase family. (336 aa) |
| | priA | Primosomal protein N; Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA; Belongs to the helicase family. PriA subfamily. (815 aa) |
| | trmD | tRNA (guanine-N1)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (248 aa) |
| | mraW | S-adenosyl-methyltransferase MraW; Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. (349 aa) |
| | nrd-2 | Ribonucleotide reductase; Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen. (761 aa) |
| | DET0347 | Conserved hypothetical protein; Identified by similarity to OMNI:NTL02ML6114. (194 aa) |
| | pheS | phenylalanyl-tRNA synthetase, alpha subunit; Identified by similarity to SP:P17921; match to protein family HMM TIGR00468; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (346 aa) |
| | pheT | phenylalanyl-tRNA synthetase, beta subunit; Identified by similarity to SP:P17922; match to protein family HMM PF01588; match to protein family HMM PF03483; match to protein family HMM TIGR00472; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (809 aa) |
| | proS | prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (569 aa) |
| | rsmI | Tetrapyrrole methylase family protein; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. (274 aa) |
| | metG | methionyl-tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (553 aa) |
| | DET0392 | AP endonuclease, family 2 protein. (276 aa) |
| | DET0395 | Glycoprotease family protein/hydrolase, beta-phosphoglucomutase family; Identified by match to protein family HMM PF00814; match to protein family HMM TIGR01509; match to protein family HMM TIGR01549; match to protein family HMM TIGR02009. (456 aa) |
| | DET0396 | Identified by similarity to GP:29894046; match to protein family HMM TIGR00150. (163 aa) |
| | ksgA | Dimethyladenosine transferase; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (291 aa) |
| | DET0409 | polyA polymerase family protein; Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. (418 aa) |
| | valS | valyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (880 aa) |
| | rnj | Metallo-beta-lactamase family protein; An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay. (551 aa) |
| | uvrB | Excinuclease ABC, B subunit; The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate [...] (668 aa) |
| | ruvA | Holliday junction DNA helicase RuvA; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. (194 aa) |
| | ruvC | Crossover junction endodeoxyribonuclease RuvC; Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group. (165 aa) |
| | rpoA | DNA-directed RNA polymerase, alpha subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (330 aa) |
| | truA | tRNA pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (248 aa) |
| | holB | DNA polymerase III, delta prime subunit; Identified by similarity to SP:P37540. (339 aa) |
| | DET0537 | HNH endonuclease domain protein. (177 aa) |
| | dnaB | Replicative DNA helicase; Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. Belongs to the helicase family. DnaB subfamily. (458 aa) |
| | uvrA | Excinuclease ABC, A subunit; The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate. (946 aa) |
| | DET0549 | Exonuclease SbcC, putative; Identified by similarity to SP:P13458. (859 aa) |
| | rpoD | RNA polymerase sigma factor RpoD; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. (520 aa) |
| | dnaG | DNA primase; RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Belongs to the DnaG primase family. (590 aa) |
| | rimM | 16S rRNA processing protein RimM; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (167 aa) |
| | nfi | Endonuclease V; DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. (221 aa) |
| | serS | seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (413 aa) |
| | lysS | lysyl-tRNA synthetase; Identified by match to protein family HMM TIGR00499; Belongs to the class-II aminoacyl-tRNA synthetase family. (498 aa) |
| | recO | Recombination protein RecO; Involved in DNA repair and RecF pathway recombination. (253 aa) |
| | recR | Recombination protein RecR; May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO. (204 aa) |
| | dnaX | DNA polymerase III, gamma and tau subunits, putative; DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. (559 aa) |
| | ligA | DNA ligase, NAD-dependent; DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. (677 aa) |
| | tyrS | tyrosyl-tRNA synthetase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. (396 aa) |
| | rpoB | DNA-directed RNA polymerase, beta subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1272 aa) |
| | rpoC | DNA-directed RNA polymerase, beta' subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1295 aa) |
| | ruvB | Holliday junction DNA helicase RuvB; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. (349 aa) |
| | queH | Conserved hypothetical protein; Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr). (182 aa) |
| | DET0611 | Conserved hypothetical protein; Identified by similarity to GP:5199108. (224 aa) |
| | nrd-3 | Ribonucleotide reductase; Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen. (599 aa) |
| | aspS | aspartyl-tRNA synthetase; Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn); Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (598 aa) |
| | xerC | Tyrosine recombinase XerC; Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC- XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids. (307 aa) |
| | topA | DNA topoisomerase I; Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supe [...] (703 aa) |
| | miaA | tRNA delta(2)-isopentenylpyrophosphate transferase; Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A); Belongs to the IPP transferase family. (325 aa) |
| | thrS | threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (582 aa) |
| | gidB | Methyltransferase GidB; Specifically methylates the N7 position of a guanine in 16S rRNA; Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family. (253 aa) |
| | trmU | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. (357 aa) |
| | rnhB | Ribonuclease HII; Endonuclease that specifically degrades the RNA of RNA-DNA hybrids; Belongs to the RNase HII family. (211 aa) |
| | glyS | glycyl-tRNA synthetase; Catalyzes the attachment of glycine to tRNA(Gly). Belongs to the class-II aminoacyl-tRNA synthetase family. (433 aa) |
| | sbcD | Nuclease SbcCD, D subunit; SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity; Belongs to the SbcD family. (415 aa) |
| | DET0789 | Pseudouridine synthase, RsuA family; Identified by similarity to SP:Q55578; match to protein family HMM PF00849; match to protein family HMM TIGR00093; Belongs to the pseudouridine synthase RsuA family. (249 aa) |
| | DET0822 | Conserved hypothetical protein; Identified by similarity to SP:Q9UX79; match to protein family HMM PF01951. (138 aa) |
| | rtcB | Conserved hypothetical protein; Identified by similarity to OMNI:AF0862; match to protein family HMM PF01139; Belongs to the RtcB family. (486 aa) |
| | DET0869 | lysyl-tRNA synthetase-related protein; Belongs to the class-II aminoacyl-tRNA synthetase family. (309 aa) |
| | DET0879 | Identified by match to protein family HMM PF01844. (182 aa) |
| | DET0883 | Site-specific recombinase, phage integrase family; Belongs to the 'phage' integrase family. (435 aa) |
| | DET0934 | Hydrolase, TatD family; Identified by match to protein family HMM TIGR00010. (264 aa) |
| | nth | Endonuclease III; DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'- phosphate. (218 aa) |
| | DET0950 | Conserved domain protein; Identified by similarity to OMNI:NTL01SS00193. (66 aa) |
| | DET0962 | tRNA modification enzyme, MiaB family; Identified by match to protein family HMM TIGR00089; match to protein family HMM TIGR01579. (413 aa) |
| | recJ | single-stranded-DNA-specific exonuclease RecJ; Identified by match to protein family HMM PF01368; match to protein family HMM PF02272; match to protein family HMM TIGR00644. (566 aa) |
| | pnp | Polyribonucleotide nucleotidyltransferase; Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction. (720 aa) |
| | truB | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (300 aa) |
| | rbfA | Ribosome-binding factor A; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (118 aa) |
| | nusA | N utilization substance protein A; Participates in both transcription termination and antitermination. (491 aa) |
| | nusG | Transcription antitermination protein NusG; Participates in transcription elongation, termination and antitermination. (177 aa) |
| | rnc | Ribonuclease III; Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. (237 aa) |
| | ileS | isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. (1014 aa) |
| | rnpA | Ribonuclease P protein component; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (120 aa) |
| | ssb | Single-strand binding protein; Identified by match to protein family HMM TIGR00621. (135 aa) |
| | recN | DNA repair protein RecN; May be involved in recombinational repair of damaged DNA. (588 aa) |
| | DET1065 | RNA methyltransferase, TrmA family; Identified by match to protein family HMM PF05958; match to protein family HMM TIGR00479; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. (395 aa) |
| | DET1067 | Site-specific recombinase, phage integrase family. (518 aa) |
| | DET1068 | Site-specific recombinase, phage integrase family, truncation. (138 aa) |
| | DET1069 | Site-specific recombinase, phage integrase family. (519 aa) |
| | DET1092 | DNA methylase. (421 aa) |
| | DET1094 | HNH endonuclease domain protein. (115 aa) |
| | DET1097 | Conserved hypothetical protein; Identified by similarity to OMNI:EF0329. (95 aa) |
| | DET1100 | DNA-dependent DNA polymerase, family A. (662 aa) |
| | DET1112 | Type III restriction-modification system, methylase subunit; Identified by similarity to SP:P40814. (587 aa) |
| | DET1114 | Type III restriction-modification system, restriction endonuclease subunit; Identified by similarity to SP:P08764. (1019 aa) |
| | DET1115 | Type II restriction endonuclease, putative. (333 aa) |
| | mutL | DNA mismatch repair protein, MutL/HexB family; This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex. (566 aa) |
| | xerD | Tyrosine recombinase XerD; Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC- XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids. (302 aa) |
| | uvrC | Excinuclease ABC, C subunit; The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision. (607 aa) |
| | mutS | DNA mismatch repair protein MutS; This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity. (858 aa) |
| | dnaN | DNA polymerase III, beta subunit; Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of [...] (378 aa) |
| | recG | ATP-dependent DNA helicase RecG; Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y- DNA); Belongs to the helicase family. RecG subfamily. (740 aa) |
| | DET1269 | HNH endonuclease domain protein. (232 aa) |
| | argS | arginyl-tRNA synthetase; Identified by match to protein family HMM PF03485; match to protein family HMM TIGR00456. (556 aa) |
| | nusB | N utilization substance protein B; Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. (143 aa) |
| | mfd | Transcription-repair coupling factor; Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site; In the C-terminal section; belongs to the helicase family. RecG subfamily. (1148 aa) |
| | rph | Ribonuclease PH; Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. (239 aa) |
| | DET1301 | ISDet4, transposase; Required for the transposition of the insertion element. (413 aa) |
| | DET1309 | ATPase, AAA family. (457 aa) |
| | trpS | tryptophanyl-tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (323 aa) |
| | DET1348 | RNA polymerase sigma-70 factor, ECF subfamily; Identified by similarity to SP:Q45585; Belongs to the sigma-70 factor family. ECF subfamily. (179 aa) |
| | gltX | glutamyl-tRNA synthetase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). (484 aa) |
| | ybeY | Conserved hypothetical protein TIGR00043; Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. (172 aa) |
| | DET1375 | Ribosomal large subunit pseudouridine synthase, RluD subfamily; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (300 aa) |
| | miaB | tRNA-i(6)A37 thiotransferase enzyme MiaB; Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6- (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. (418 aa) |
| | mutM | formamidopyrimidine-DNA glycosylase; Identified by match to protein family HMM PF01149; match to protein family HMM TIGR00577. (270 aa) |
| | polA | DNA-directed DNA polymerase I; In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity; Belongs to the DNA polymerase type-A family. (903 aa) |
| | dnaJ | Co-chaperone protein DnaJ; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ [...] (356 aa) |
| | dnaJ-2 | DnaJ family protein; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK [...] (330 aa) |
| | tilS | PP-loop family protein; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Belongs to the tRNA(Ile)-lysidine synthase family. (470 aa) |
| | gcp | O-sialoglycoprotein endopeptidase; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction; Belongs to the KAE1 / TsaD family. (326 aa) |
| | ogt | methylated-DNA--protein-cysteine methyltransferase; Identified by match to protein family HMM TIGR00589. (186 aa) |
| | dnaE | DNA polymerase III, alpha subunit; Identified by match to protein family HMM TIGR00594; match to protein family HMM TIGR01369. (1170 aa) |
| | DET1474 | Site-specific recombinase, phage integrase family; Belongs to the 'phage' integrase family. (386 aa) |
| | DET1508 | ISDet3, transposase orfA; Identified by similarity to SP:P24580; match to protein family HMM PF01527. (92 aa) |
| | DET1552 | Resolvase domain protein. (554 aa) |
| | recA | RecA; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage; Belongs to the RecA family. (337 aa) |
| | rny | KH domain/HDIG domain protein; Endoribonuclease that initiates mRNA decay. Belongs to the RNase Y family. (527 aa) |
| | DET1612 | DNA repair protein, HhH-GPD family. (220 aa) |
| | gyrA | DNA gyrase, A subunit; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (809 aa) |
| | lexA | LexA repressor; Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (212 aa) |
| | fmt | methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. (312 aa) |
