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bioF2 bioF2 glyA2 glyA2 Rv0075 Rv0075 aspC aspC metZ metZ hemL hemL Rv0812 Rv0812 cysK2 cysK2 dapC dapC serC serC metB metB glyA1 glyA1 Rv1155 Rv1155 Rv1178 Rv1178 lysA lysA thrC thrC glgP glgP cysM cysM csd csd Rv1503c Rv1503c Rv1504c Rv1504c ilvA ilvA bioA bioA bioF1 bioF1 hisC1 hisC1 argD argD gcvB gcvB Rv1864c Rv1864c Rv2148c Rv2148c ilvE ilvE cobC cobC Rv2294 Rv2294 rocD2 rocD2 rocD1 rocD1 cysK1 cysK1 gabT gabT iscS iscS lat lat Rv3329 Rv3329 metC metC alr alr gadB gadB aspB aspB hisC2 hisC2
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bioF2Putative 8-amino-7-oxononanoate synthase 2; Catalyzes the decarboxylative condensation of pimeloyl-[acyl- carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. (771 aa)
glyA2Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2); Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L- allo-threonine; Belongs to the SHMT family. (425 aa)
Rv0075Rv0075, (MTV030.19), len: 390 aa. Probable aminotransferase, similar to many class-II pyridoxal-phosphate-dependent aminotransferases (MALY/PATB subfamily). Also similar to other proteins from Mycobacterium tuberculosis e.g. Rv2294, Rv0858c, etc. (390 aa)
aspCRv0337c, (MTCY279.04c), len: 429 aa. Probable aspC,aspartate aminotransferase (transaminase A), equivalent to CAC32019.1|AL583925 probable aspartate aminotransferase from Mycobacterium leprae (437 aa). Also highly similar to many e.g. Q48143|U32823 aspartate aminotransferase (404 aa), FASTA scores: opt: 1646, E(): 0, (57.2% identity in 404 aa overlap). Also some similarity to Rv3565|MTCY06G11.12 from Mycobacterium tuberculosis FASTA score: (27.2% identity in 383 aa overlap). Belongs to class-I of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. (429 aa)
metZProbable O-succinylhomoserine sulfhydrylase MetZ (OSH sulfhydrylase); Catalyzes the formation of L-homocysteine from O-succinyl-L- homoserine (OSHS) and hydrogen sulfide. (406 aa)
hemLRv0524, (MTCY25D10.03), len: 462 aa. Probable hemL,glutamate-1-semialdehyde 2,1-aminomutase, equivalent to P46716|GSA_MYCLE glutamate-1-semialdehyde 2,1-aminomutase from Mycobacterium leprae (446 aa), FASTA scores: opt: 1532, E(): 0, (82.6% identity in 460 aa overlap). Also highly similar to others e.g. Q9F2S0|GSA_STRCO from Streptomyces coelicolor (438 aa); Q06774|GSA_PROFR from Propionibacterium freudenreichii (441 aa); etc. Contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofact [...] (462 aa)
Rv0812Rv0812, (MTV043.04), len: 289 aa. Probable amino acid aminotransferase, similar to other amino acid aminotransferases, generelly class-IV of pyridoxal-phosphate-dependent aminotransferases, and especially ILVE proteins and PABC proteins e.g. B76065.1|AL157953 putative aminotransferase from Streptomyces coelicolor (273 aa); NP_069766.1|NC_000917 branched-chain amino acid aminotransferase (ilvE) from Archaeoglobus fulgidus (290 aa); P54692|DAAA_BACLI D-alanine aminotransferase from Bacillus licheniformis (283 aa); P28305|PABC_ECOLI|B1096 4-amino-4-deoxychorismate lyase (ADC lyase) From E [...] (289 aa)
cysK2S-sulfocysteine synthase; Catalyzes the synthesis of S-sulfocysteine, utilizing O- phosphoserine (OPS) and thiosulfate as substrates. To a lesser extent, can also use sulfide as donor substrate, producing L-cysteine. CysK2 thus provides a third metabolic route to cysteine, either directly using sulfide as donor or indirectly via S-sulfocysteine. S- sulfocysteine might also act as a signaling molecule triggering additional responses in redox defense in the pathogen upon exposure to reactive oxygen species during intracellular survival or dormancy. Cannot utilize thiocarboxylated CysO as [...] (372 aa)
dapCProbable N-succinyldiaminopimelate aminotransferase DapC (DAP-at); Involved in the lysine biosynthetic pathways. It catalyzes the transfer of an amino group from L-glutamate to N-succinyl-2-l- amino-6-oxoheptanedioate (N-succinyl-2-l-amino-6-ketopimelate) in a PLP-dependent reaction, yielding as products N-succinyl-l-2,6- diaminoheptanedioate (N-succinyl-diaminopimelate) and 2-oxoglutarate (Probable); Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. (397 aa)
serCPossible phosphoserine aminotransferase SerC (PSAT); Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. (376 aa)
metBCystathionine gamma-synthase MetB (CGS) (O-succinylhomoserine [thiol]-lyase); Catalyzes the formation of L-cystathionine from O-succinyl-L- homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction (By similarity). In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia. (388 aa)
glyA1Serine hydroxymethyltransferase 1 GlyA1; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L- allo-threonine; Belongs to the SHMT family. (426 aa)
Rv1155F420H(2)-dependent reductase Rv1155; F420H(2)-dependent reductase able to catalyze the reduction of biliverdin-IXalpha to bilirubin-IXalpha in vitro. However, kinetic parameters show that it is less efficient than the biliverdin reductase Rv2074 and suggest biliverdin-IXalpha is unlikely to be the native substrate of Rv1155, which probably catalyzes the reduction of an alternative molecule in vivo. Binds coenzyme F420, but does not bind FMN or other flavins. Cannot use pyridoxine 5'-phosphate, pyridoxamine 5'-phosphate, pyridoxal 5'- phosphate (PLP), the anti-tuberculosis drug PA-824 o [...] (147 aa)
Rv1178Rv1178, (MTV005.14), len: 362 aa. Probable aminotransferase, weak similarity to many aspartate aminotransferases e.g. Q55679|D64000 SLL0006 aspartate aminotransferase from Synechocystis sp. (394 aa), FASTA scores: opt: 218, E(): 1.3e-25, (32.5% identity in 379 aa overlap). Contains PS00105 Aminotransferases class-I pyridoxal-phosphate attachment site. Also similar to Mycobacterium tuberculosis aminotransferases Rv2294,Rv0075, etc. (362 aa)
lysADiaminopimelate decarboxylase LysA (DAP decarboxylase); Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine (Probable). Is essential for the viability of M.tuberculosis in the host. (447 aa)
thrCThreonine synthase ThrC (ts); Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. (360 aa)
glgPProbable glycogen phosphorylase GlgP; Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity). (863 aa)
cysMO-phosphoserine sulfhydrylase; Catalyzes the formation of a covalent CysO-cysteine adduct via a sulfur transfer, using the thiocarboxylated sulfur carrier protein CysO-COSH as sulfur donor and O-phospho-L-serine (OPS) as sulfur acceptor. Can also use sodium sulfide as sulfur donor in vitro, albeit with less efficiency, but not thiosulfate or thio-nitro- benzoate. O-acetylserine (OAS) is a very poor substrate in comparison with OPS. May be of particular importance for cysteine biosynthesis in the persistent phase of M.tuberculosis; Belongs to the cysteine synthase/cystathionine beta- sy [...] (323 aa)
csdProbable cysteine desulfurase Csd; Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily. (417 aa)
Rv1503cRv1503c, (MTCY277.25c), len: 182 aa. Conserved hypothetical protein, similar to C-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 565,E(): 0, (49.4% identity in 170 aa overlap); Rv1503c and Rv1504c are both similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. (182 aa)
Rv1504cRv1504c, (MTCY277.26c), len: 199 aa. Conserved hypothetical protein, similar to N-terminal region of P27833|RFFA_ECOLI lipopolysaccharide biosynthesis protein from Escherichia coli (376 aa), FASTA scores: opt: 863,E(): 0, (68.0% identity in 194 aa overlap); Rv1503c and Rv1504c are similar to RFFA_ECOLI but are separated by a stop codon, sequence appears to be correct so possible pseudogene. (199 aa)
ilvAProbable threonine dehydratase IlvA; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity). (429 aa)
bioAAdenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA; Catalyzes the reversible transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor. Can also use sinefungin as substrate. (437 aa)
bioF18-amino-7-oxononanoate synthase 1; Catalyzes the decarboxylative condensation of pimeloyl-[acyl- carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide (By similarity). Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate. To a lesser extent, can also utilize D-alanine instead of L-alanine as substrate. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily. (386 aa)
hisC1Rv1600, (MTCY336.04c), len: 380 aa. Probable hisC1,histidinol-phosphate aminotransferase O06591. Similar to many e.g. HIS8_STRCO|P16246 from Streptomyces coelicolor (369 aa), FASTA results: opt: 1353, E(): 0, (59.0% identity in 356 aa overlap). Some similarity to other Mycobacterium tuberculosis aminotransferases e.g. Rv3772|MTCY13D12.06,FASTA results: E(): 7.4e-25, (33.7% identity in 365 aa overlap). Contains aminotransferases class-II pyridoxal-phosphate attachment site (PS00599). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. Note that previously known as hisC. (380 aa)
argDRv1655, (MTCY06H11.20), len: 400 aa. Probable argD,Acetylornithine aminotransferase, similar to ARGD_ECOLI|P18335 (406 aa), FASTA scores: opt: 958, E(): 0,(38.6% identity in 404 aa overlap), contains PS00600 Aminotransferases class-III pyridoxal-phosphate attachment site. Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. (400 aa)
gcvBProbable glycine dehydrogenase GcvB (glycine decarboxylase) (glycine cleavage system P-protein); The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (By similarity); Belongs to the GcvP family. (941 aa)
Rv1864cConserved protein; Rv1864c, (MTCY359.09), len: 251 aa. Conserved protein. Similar to other hypothetical proteins e.g. AL031317|SC6G4.43 from Streptomyces coelicolor cosmid 6G (233 aa), FASTA scores: opt: 716, E(): 0, (54.4% identity in 215 aa overlap); also P43976|YIIM_HAEIN hypothetical protein hi0278 (221 aa), FASTA scores: opt: 223, E(): 3.8e-08, (29.5% identity in 173 aa overlap). (251 aa)
Rv2148cConserved protein; Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis. (258 aa)
ilvEBranched-chain amino acid transaminase IlvE; Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to their efficiency as amino donor: Leu > Ile > Val. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. (368 aa)
cobCRv2231c, (MTCY427.12c), len: 364 aa. Possible cobC,aminotransferase. Note that initiation codon uncertain. Similar to CobC aminotransferases e.g. sp|P21633|COBC_PSEDE COBC protein (333 aa) opt: 277, E(): 1.7e-11; 28.8% identity in 313 aa overlap and also to e.g. SW:HIS8_ECOLI P06986 histidinol-phosphate aminotransferase (27.0% identity in 289 aa overlap), contains PS00105 aminotransferases class-I pyridoxal-phosphate attachment site. Real Mycobacterium tuberculosis histidinol-phosphate aminotransferase, hisC, is Rv1600 (MTCY336.04c); Belongs to the class-I pyridoxal-phosphate-dependent [...] (364 aa)
Rv2294Rv2294, (MTCY339.16c), len: 407 aa. Probable aminotransferase, similar to others in M. tuberculosis e.g. MTV030_19, also similar to PATB_BACSU|Q08432 putative aminotransferase b from Bacillus subtilis (387 aa), FASTA scores: opt: 563, E(): 2.8e-29, (31.4% identity in 408 aa overlap); and to MALY_ECOLI|P23256 maly protein from Escherichia coli (390 aa), FASTA scores: opt: 530, E(): 3.6e-27, (31.3% identity in 384 aa overlap). Belongs to class-II of pyridoxal-phosphate-dependent aminotransferases. (407 aa)
rocD2Rv2321c, (MTCY3G12.13), len: 181 aa. Probable rocD2,ornithine aminotransferase, highly similar to C-terminal region of other ornithine aminotransferases, e.g. Q9FC90|ROCD from Streptomyces coelicolor (407 aa), FASTA scores: opt: 628, E(): 1.2e-32, (55.35% identity in 168 aa overlap); P3802|OAT_BACSU|ROCD from Bacillus subtilis (401 aa), FASTA scores: opt: 477, E(): 4.3e-23, (42.1% identity in 178 aa overlap); BAB42057|ROCD|SA0818 from Staphylococcus aureus subsp. aureus N315 (396 aa), FASTA scores: opt: 437, E(): 1.5e-20, (41.3% identity in 170 aa overlap); etc. Contains PS00600 Aminot [...] (181 aa)
rocD1Rv2322c, (MTCY3G12.12), len: 221 aa. Probable rocD1,ornithine aminotransferase, highly similar to N-terminal region of other ornithine aminotransferases, e.g. Q9FC90|ROCD from Streptomyces coelicolor (407 aa), FASTA scores: opt: 770, E(): 8.7e-40, (55.7% identity in 201 aa overlap); BAB42057|ROCD|SA0818 from Staphylococcus aureus subsp. aureus N315 (396 aa) FASTA scores: opt: 632, E(): 2.2e-31, (46.1% identity in 208 aa overlap); P38021|OAT_BACSU|ROCD from Bacillus subtilis (401 aa),FASTA scores: opt: 626, E(): 5.1e-31, (43.1% identity in 218 aa overlap); etc. Belongs to class-III of p [...] (221 aa)
cysK1O-acetylserine sulfhydrylase; Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide. Belongs to the cysteine synthase/cystathionine beta- synthase family. (310 aa)
gabT4-aminobutyrate aminotransferase; Rv2589, (MTCY227.12c), len: 449 aa. Probable gabT,4-aminobutyrate aminotransferase, equivalent to P40829|GABT_MYCLE|ML0485|MLCB1259.03c|B1177_F2_67 4-aminobutyrate aminotransferase (446 aa), FASTA scores: opt: 2468, E(): 4.5e-141, (83.75% identity in 449 aa overlap). Also highly similar to others e.g. O86823|GABT from Streptomyces coelicolor (444 aa), FASTA scores: opt: 1832, E(): 8e-103, (63.9% identity in 443 aa overlap); AAK79395|CAC1427 from Clostridium acetobutylicum (445 aa),FASTA scores: opt: 1283, E(): 8.4e-70, (45.75% identity in 433 aa overla [...] (449 aa)
iscSIscS-like cysteine desulfurase; Catalyzes the removal of elemental sulfur from cysteine to produce alanine (Probable). Participates in the biosynthesis of metalloclusters by providing the inorganic sulfur required for Fe-S core formation. One acceptor is Whib3, on which this enzyme assembles a 4Fe-4S cluster. It can use both L-cysteine and L-selenocysteine as substrates. (393 aa)
latRv3290c, (MTCY71.30), len: 449 aa. Probable lat,lysine-epsilon aminotransferase, similar to Q05174|LAT_NOCLA from Nocardia lactamdurans (450 aa), FASTA scores: opt: 1702, E(): 1.1e-99, (60.35% identity in 439 aa overlap); and Q01767|Q53823|LAT_STRCL from Streptomyces clavuligerus (457 aa), FASTA scores: opt: 1676, E(): 4.9e-98, (60.15% identity in 434 aa overlap). Also some similarity to 4-aminobutyrate aminotransferase proteins (gamma-amino-N-butyrate transaminases). Belongs to class-III of pyridoxal-phosphate-dependent aminotransferases. Cofactor: pyridoxal phosphate. (449 aa)
Rv3329Probable aminotransferase; Probable aminotransferase. (438 aa)
metCO-acetylhomoserine sulfhydrylase MetC; Rv3340, (MTV016.40), len: 449 aa. Probable metC,O-acetyl-L-homoserine sulfhydrylase, highly similar to many e.g. Q9K9P2|BH2603 O-acetylhomoserine sulfhydrylase from Bacillus halodurans (430 aa), FASTA scores: opt: 1716, E(): 3.3e-97, (60.45% identity in 425 aa overlap); Q9HUE4|METY|PA5025 homocysteine synthase from Pseudomonas aeruginosa (425 aa), FASTA scores: opt: 1517, E(): 4.4e-85,(56.95% identity in 425 aa overlap); Q9WZY4|TM0882 O-acetylhomoserine sulfhydrylase from Thermotoga maritima (430 aa), FASTA scores: opt: 1488, E(): 2.6e-83, (55.75% [...] (449 aa)
alrAlanine racemase Alr; Catalyzes the interconversion of L-alanine and D-alanine. D- alanine plays a key role in peptidoglycan cross-linking. (408 aa)
gadBRv3432c, (MTCY77.04c), len: 460 aa. Probable gadB,glutamate decarboxylase, similar to many e.g. P73043|gad|SLL1641 from Synechocystis sp. strain PCC 6803 (467 aa), FASTA scores: opt: 1684, E(): 6.2e-99, (55.35% identity in 457 aa overlap); Q9X8J5|SCE9.23 from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1650,E(): 8.9e-97, (57.4% identity in 446 aa overlap); Q9AQU4|gad from Oryza sativa (Rice) (501 aa), FASTA scores: opt: 1498, E(): 3.7e-87, (51.6% identity in 432 aa overlap); Q07346|DCE_PETHY from Petunia hybrida (Petunia) (500 aa), FASTA scores: opt: 1485, E(): 2.5e-86, (51.15 [...] (460 aa)
aspBAminotransferase; Rv3565, (MTCY06G11.12), len: 388 aa. Possible aspB,aspartate aminotransferase, similar to many e.g. Q9A5J2|CC2455 aminotransferase class I from Caulobacter crescentus (381 aa), FASTA scores: opt: 1112, E(): 1e-61,(45.85% identity in 384 aa overlap); Q9HV76|PA4722 probable aminotransferase from Pseudomonas aeruginosa (390 aa),FASTA scores: opt: 863, E(): 3.1e-46, (37.2% identity in 390 aa overlap); Q9RWP3|DR0623 aspartate aminotransferase from Deinococcus radiodurans (388 aa), FASTA scores: opt: 713, E(): 6.3e-37, (35.5% identity in 383 aa overlap); Q9HQK2|ASPC2|VNG112 [...] (388 aa)
hisC2Putative phenylalanine aminotransferase; May catalyze the transamination reaction in phenylalanine biosynthesis; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. (353 aa)
Your Current Organism:
Mycobacterium tuberculosis H37Rv
NCBI taxonomy Id: 83332
Other names: M. tuberculosis H37Rv, Mycobacterium sp. H37Rv, Mycobacterium tuberculosis str. H37Rv, Mycobacterium tuberculosis strain H37Rv
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