node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
BRADO1163 | thyA | BRADO1163 | BRADO5578 | Putative pyrimidine reductase; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative enzyme. | Thymidylate synthase (TS) (TSase); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.888 |
BRADO2166 | dut | BRADO2166 | BRADO0078 | Putative 5'-nucleotidase; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; enzyme; Belongs to the 5'-nucleotidase family. | Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (dUTP pyrophosphatase); This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | 0.900 |
BRADO2166 | surE | BRADO2166 | BRADO4042 | Putative 5'-nucleotidase; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; enzyme; Belongs to the 5'-nucleotidase family. | 5'-nucleotidase SurE; Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates; Belongs to the SurE nucleotidase family. | 0.916 |
BRADO2166 | thyA | BRADO2166 | BRADO5578 | Putative 5'-nucleotidase; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; enzyme; Belongs to the 5'-nucleotidase family. | Thymidylate synthase (TS) (TSase); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.907 |
BRADO2166 | tmk | BRADO2166 | BRADO3827 | Putative 5'-nucleotidase; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; enzyme; Belongs to the 5'-nucleotidase family. | Thymidylate kinase; Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis; Belongs to the thymidylate kinase family. | 0.933 |
dut | BRADO2166 | BRADO0078 | BRADO2166 | Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (dUTP pyrophosphatase); This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | Putative 5'-nucleotidase; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; enzyme; Belongs to the 5'-nucleotidase family. | 0.900 |
dut | surE | BRADO0078 | BRADO4042 | Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (dUTP pyrophosphatase); This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | 5'-nucleotidase SurE; Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates; Belongs to the SurE nucleotidase family. | 0.906 |
dut | thyA | BRADO0078 | BRADO5578 | Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (dUTP pyrophosphatase); This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | Thymidylate synthase (TS) (TSase); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.977 |
dut | tmk | BRADO0078 | BRADO3827 | Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (dUTP pyrophosphatase); This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | Thymidylate kinase; Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis; Belongs to the thymidylate kinase family. | 0.955 |
folA | gcvT | BRADO5576 | BRADO4982 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Glycine cleavage system T-protein (aminomethyltransferase); Function of strongly homologous gene; enzyme. | 0.913 |
folA | glyA | BRADO5576 | BRADO4430 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.930 |
folA | thyA | BRADO5576 | BRADO5578 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Thymidylate synthase (TS) (TSase); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.999 |
folD | gcvT | BRADO0277 | BRADO4982 | Bifunctional: 5,10-methylene-tetrahydrofolate dehydrogenase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | Glycine cleavage system T-protein (aminomethyltransferase); Function of strongly homologous gene; enzyme. | 0.963 |
folD | glyA | BRADO0277 | BRADO4430 | Bifunctional: 5,10-methylene-tetrahydrofolate dehydrogenase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.988 |
folD | metF | BRADO0277 | BRADO0995 | Bifunctional: 5,10-methylene-tetrahydrofolate dehydrogenase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 5,10-methylenetetrahydrofolate reductase; Function of homologous gene experimentally demonstrated in an other organism; enzyme; Belongs to the methylenetetrahydrofolate reductase family. | 0.944 |
folD | thyA | BRADO0277 | BRADO5578 | Bifunctional: 5,10-methylene-tetrahydrofolate dehydrogenase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | Thymidylate synthase (TS) (TSase); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.946 |
gcvT | folA | BRADO4982 | BRADO5576 | Glycine cleavage system T-protein (aminomethyltransferase); Function of strongly homologous gene; enzyme. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.913 |
gcvT | folD | BRADO4982 | BRADO0277 | Glycine cleavage system T-protein (aminomethyltransferase); Function of strongly homologous gene; enzyme. | Bifunctional: 5,10-methylene-tetrahydrofolate dehydrogenase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.963 |
gcvT | glyA | BRADO4982 | BRADO4430 | Glycine cleavage system T-protein (aminomethyltransferase); Function of strongly homologous gene; enzyme. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.997 |
gcvT | metF | BRADO4982 | BRADO0995 | Glycine cleavage system T-protein (aminomethyltransferase); Function of strongly homologous gene; enzyme. | 5,10-methylenetetrahydrofolate reductase; Function of homologous gene experimentally demonstrated in an other organism; enzyme; Belongs to the methylenetetrahydrofolate reductase family. | 0.931 |