Phosphomannose isomerase / guanosine 5'-diphospho-D-mannose pyrophosphorylase; Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics; Belongs to the mannose-6-phosphate isomerase type 2 family.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Alginate-c5-mannuronan-epimerase AlgG; Catalyzes the epimerization of beta-D-mannuronate to alpha-L- guluronate during the synthesis of the linear polysaccharide alginate. In addition, is part of a periplasmic protein complex that protects alginate from degradation by AlgL by channeling the newly formed alginate polymer through a scaffold that transfers the alginate polymer through the periplasmic space to the outer membrane secretin AlgE.

Alginate biosynthesis protein AlgX; Plays two roles in the biosynthesis of the exopolysaccharide alginate: protects alginate from degradation as the polymer traverses the periplasm, and also plays a role in its O-acetylation. Acetylation of alginate causes the cells in the biofilm to adhere better to lung epithelium, form microcolonies, and resist the effects of the host immune system and/or antibiotics. Displays a low acetylesterase activity in vitro using a pseudosubstrate, 3-carboxyumbelliferyl acetate. Probably has acetyltransferase activity in vivo. Belongs to the AlgX family.

Alginate biosynthetic protein AlgK precursor; May be involved in the polymerization of mannuronate to alginate.

Poly(beta-d-mannuronate) lyase precursor AlgL; Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. May serve to degrade mislocalized alginate that is trapped in the periplasmic space. Acts preferentially on non-acetylated alginate or its precursor mannuronan. Is able to catalyze cleavage adjacent to either mannuronate or guluronate residues in alginate. Exhaustive digestion of alginate by AlgL generates dimeric and trimeric products. In addition to its enzymatic function, AlgL appears [...]

Alginate production outer membrane protein AlgE precursor; Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.

Alginate o-acetyltransferase AlgJ; Together with AlgI and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization-transport complex and adds acetyl groups at the O-2 and O-3 positions of mannuronate residues. Acetylation of alginate is important for the architecture of biofilms and increases resistance to opsonic killing in the host.

PslB; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa; Belongs to the mannose-6-phosphate isomerase type 2 family.