node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
ACS39514.1 | rutA | MexAM1_META1p1665 | MexAM1_META1p1664 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | 0.626 |
ACS39514.1 | rutB | MexAM1_META1p1665 | MexAM1_META1p1663 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.592 |
ACS39514.1 | rutC | MexAM1_META1p1665 | MexAM1_META1p1662 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.522 |
ACS39514.1 | rutD | MexAM1_META1p1665 | MexAM1_META1p1661 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Putative enzyme of alternative pyrimidine degradation pathway, putative alpha/beta hydrolase; May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation. Belongs to the AB hydrolase superfamily. Hydrolase RutD family. | 0.577 |
ACS39514.1 | rutF | MexAM1_META1p1665 | MexAM1_META1p1660 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Flavin reductase domain protein, FMN-binding; Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway; Belongs to the non-flavoprotein flavin reductase family. RutF subfamily. | 0.547 |
ACS40697.1 | rutB | MexAM1_META1p2945 | MexAM1_META1p1663 | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.537 |
ACS41746.1 | rutB | MexAM1_META1p4092 | MexAM1_META1p1663 | glutamyl-tRNA(Gln) amidotransferase (subunit A); Function of strongly homologous gene; enzyme. | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.921 |
nadE | nnrE | MexAM1_META1p4425 | MexAM1_META1p2375 | NAD(+) synthase (glutamine-hydrolyzing) with a nitrilase-like domain; Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source. | Putative carbohydrate kinase; Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of [...] | 0.510 |
nadE | rutB | MexAM1_META1p4425 | MexAM1_META1p1663 | NAD(+) synthase (glutamine-hydrolyzing) with a nitrilase-like domain; Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source. | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.419 |
nnrE | nadE | MexAM1_META1p2375 | MexAM1_META1p4425 | Putative carbohydrate kinase; Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of [...] | NAD(+) synthase (glutamine-hydrolyzing) with a nitrilase-like domain; Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source. | 0.510 |
nnrE | rutB | MexAM1_META1p2375 | MexAM1_META1p1663 | Putative carbohydrate kinase; Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of [...] | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.532 |
nnrE | rutC | MexAM1_META1p2375 | MexAM1_META1p1662 | Putative carbohydrate kinase; Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of [...] | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.445 |
rutA | ACS39514.1 | MexAM1_META1p1664 | MexAM1_META1p1665 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | 0.626 |
rutA | rutB | MexAM1_META1p1664 | MexAM1_META1p1663 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.995 |
rutA | rutC | MexAM1_META1p1664 | MexAM1_META1p1662 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.931 |
rutA | rutD | MexAM1_META1p1664 | MexAM1_META1p1661 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | Putative enzyme of alternative pyrimidine degradation pathway, putative alpha/beta hydrolase; May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation. Belongs to the AB hydrolase superfamily. Hydrolase RutD family. | 0.937 |
rutA | rutF | MexAM1_META1p1664 | MexAM1_META1p1660 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | Flavin reductase domain protein, FMN-binding; Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway; Belongs to the non-flavoprotein flavin reductase family. RutF subfamily. | 0.987 |
rutA | rutF-2 | MexAM1_META1p1664 | MexAM1_META1p3229 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | flavin:NADH oxidoreductase, putative alternative pyrimidine degradation enzyme; Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway; Belongs to the non-flavoprotein flavin reductase family. RutF subfamily. | 0.959 |
rutB | ACS39514.1 | MexAM1_META1p1663 | MexAM1_META1p1665 | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | 0.592 |
rutB | ACS40697.1 | MexAM1_META1p1663 | MexAM1_META1p2945 | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | 0.537 |