3-methylcrotonoyl-CoA carboxylase, alpha subunit; IMG reference gene:2512954454; PFAM: Carbamoyl-phosphate synthase L chain, ATP binding domain; Biotin carboxylase C-terminal domain; Carbamoyl-phosphate synthase L chain, N-terminal domain; Biotin-requiring enzyme; TIGRFAM: acetyl-CoA carboxylase, biotin carboxylase subunit.

methylmalonyl-CoA epimerase; Promiscuous isomerase that catalyzes epimerization of both ethylmalonyl-CoA and methylmalonyl-CoA. Has thus a dual role in the ethylmalonyl-CoA pathway for acetyl-CoA assimilation required for R.sphaeroides growth on acetate as sole carbon source.

Phosphate acetyltransferase; IMG reference gene:2512956299; PFAM: MaoC like domain; Phosphate acetyl/butaryl transferase.

3-hydroxyacyl-CoA dehydrogenase; IMG reference gene:2512954124; PFAM: Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain.

IMG reference gene:2512957047; PFAM: Thiolase, C-terminal domain; Thiolase, N-terminal domain; TIGRFAM: acetyl-CoA acetyltransferases; 3-oxoadipyl-CoA thiolase; Belongs to the thiolase-like superfamily. Thiolase family.

acetyl-CoA carboxylase; This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.

Acetyl-coenzyme A synthetase; Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA; Belongs to the ATP-dependent AMP-binding enzyme family.

propionyl-CoA synthetase; IMG reference gene:2512953489; PFAM: Domain of unknown function (DUF3448); AMP-binding enzyme; truncated by 8 aa.

beta-methylmalyl-CoA/L-malyl-CoA lyase; Involved in the ethylmalonyl-CoA pathway for acetate assimilation. Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to yield beta-methylmalyl-CoA. It is also able to catalyze the cleavage of (S)-citramalyl-CoA to yield acetyl-CoA and pyruvate, although this reaction is not involved in the ethylmalonyl- CoA pathway.