node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
BBta_3156 | BBta_4344 | BBta_3156 | BBta_4344 | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | 0.900 |
BBta_3156 | fmt | BBta_3156 | BBta_7336 | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. | 0.900 |
BBta_3156 | folD | BBta_3156 | BBta_0269 | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.964 |
BBta_3156 | gcvT | BBta_3156 | BBta_5449 | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | 0.919 |
BBta_3156 | glyA | BBta_3156 | BBta_4649 | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.919 |
BBta_3156 | purN | BBta_3156 | BBta_3853 | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | Formyltetrahydrofolate-dependent phosphoribosylglycinamide formyltransferase; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. | 0.923 |
BBta_3156 | thyA | BBta_3156 | BBta_6100 | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.517 |
BBta_4344 | BBta_3156 | BBta_4344 | BBta_3156 | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | 0.900 |
BBta_4344 | fmt | BBta_4344 | BBta_7336 | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. | 0.925 |
BBta_4344 | folD | BBta_4344 | BBta_0269 | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.964 |
BBta_4344 | gcvT | BBta_4344 | BBta_5449 | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | 0.919 |
BBta_4344 | glyA | BBta_4344 | BBta_4649 | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.919 |
BBta_4344 | purN | BBta_4344 | BBta_3853 | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | Formyltetrahydrofolate-dependent phosphoribosylglycinamide formyltransferase; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. | 0.923 |
BBta_4344 | thyA | BBta_4344 | BBta_6100 | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.517 |
BBta_6914 | folD | BBta_6914 | BBta_0269 | 5-formyltetrahydrofolate cyclo-ligase; Evidence: Function of homologous gene experimentally demonstrated in an other organism; Belongs to the 5-formyltetrahydrofolate cyclo-ligase family. | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.940 |
BBta_6914 | glyA | BBta_6914 | BBta_4649 | 5-formyltetrahydrofolate cyclo-ligase; Evidence: Function of homologous gene experimentally demonstrated in an other organism; Belongs to the 5-formyltetrahydrofolate cyclo-ligase family. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.528 |
BBta_6914 | purN | BBta_6914 | BBta_3853 | 5-formyltetrahydrofolate cyclo-ligase; Evidence: Function of homologous gene experimentally demonstrated in an other organism; Belongs to the 5-formyltetrahydrofolate cyclo-ligase family. | Formyltetrahydrofolate-dependent phosphoribosylglycinamide formyltransferase; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. | 0.936 |
fmt | BBta_3156 | BBta_7336 | BBta_3156 | methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. | Hypothetical protein; Methionyl-tRNA formyltransferase domain; Evidence: Similar to previously reported genes of unknown function. | 0.900 |
fmt | BBta_4344 | BBta_7336 | BBta_4344 | methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. | Putative Methionyl-tRNA formyltransferase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 2 : Cytoplasmic. | 0.925 |
fmt | folD | BBta_7336 | BBta_0269 | methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.964 |