node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
BBta_2482 | dut | BBta_2482 | BBta_0084 | Putative 5'-nucleotidase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 9 : Periplasmic; Belongs to the 5'-nucleotidase family. | Deoxyuridine 5'-triphosphate nucleotidohydrolase; This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | 0.901 |
BBta_2482 | surE | BBta_2482 | BBta_4412 | Putative 5'-nucleotidase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 9 : Periplasmic; Belongs to the 5'-nucleotidase family. | 3'-nucleotidase; Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates; Belongs to the SurE nucleotidase family. | 0.916 |
BBta_2482 | thyA | BBta_2482 | BBta_6100 | Putative 5'-nucleotidase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 9 : Periplasmic; Belongs to the 5'-nucleotidase family. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.910 |
BBta_2482 | tmk | BBta_2482 | BBta_4102 | Putative 5'-nucleotidase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 9 : Periplasmic; Belongs to the 5'-nucleotidase family. | Thymidylate kinase; Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis; Belongs to the thymidylate kinase family. | 0.935 |
BBta_3878 | gcvT | BBta_3878 | BBta_5449 | Hypothetical protein; Dihydrofolate reductase domain; Evidence: Similar to previously reported genes of unknown function. | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | 0.580 |
BBta_3878 | thyA | BBta_3878 | BBta_6100 | Hypothetical protein; Dihydrofolate reductase domain; Evidence: Similar to previously reported genes of unknown function. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.908 |
dut | BBta_2482 | BBta_0084 | BBta_2482 | Deoxyuridine 5'-triphosphate nucleotidohydrolase; This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | Putative 5'-nucleotidase; Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Localization: 9 : Periplasmic; Belongs to the 5'-nucleotidase family. | 0.901 |
dut | surE | BBta_0084 | BBta_4412 | Deoxyuridine 5'-triphosphate nucleotidohydrolase; This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | 3'-nucleotidase; Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates; Belongs to the SurE nucleotidase family. | 0.908 |
dut | thyA | BBta_0084 | BBta_6100 | Deoxyuridine 5'-triphosphate nucleotidohydrolase; This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.976 |
dut | tmk | BBta_0084 | BBta_4102 | Deoxyuridine 5'-triphosphate nucleotidohydrolase; This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. | Thymidylate kinase; Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis; Belongs to the thymidylate kinase family. | 0.951 |
folA | gcvT | BBta_6098 | BBta_5449 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | 0.916 |
folA | glyA | BBta_6098 | BBta_4649 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.933 |
folA | thyA | BBta_6098 | BBta_6100 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.999 |
folD | gcvT | BBta_0269 | BBta_5449 | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | 0.961 |
folD | glyA | BBta_0269 | BBta_4649 | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.988 |
folD | metF | BBta_0269 | BBta_7060 | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 5,10-methylenetetrahydrofolate reductase; Evidence: Function of homologous gene experimentally demonstrated in an other organism; Localization: 2 : Cytoplasmic; Belongs to the methylenetetrahydrofolate reductase family. | 0.940 |
folD | thyA | BBta_0269 | BBta_6100 | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.945 |
gcvT | BBta_3878 | BBta_5449 | BBta_3878 | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | Hypothetical protein; Dihydrofolate reductase domain; Evidence: Similar to previously reported genes of unknown function. | 0.580 |
gcvT | folA | BBta_5449 | BBta_6098 | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.916 |
gcvT | folD | BBta_5449 | BBta_0269 | Glycine cleavage system T-protein (aminomethyltransferase); Evidence: Function of strongly homologous gene; Localization: 2 : Cytoplasmic. | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.961 |