node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
ACS23599.1 | ACS24377.1 | GWCH70_0708 | GWCH70_1582 | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.937 |
ACS23599.1 | ACS25267.1 | GWCH70_0708 | GWCH70_2572 | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | TIGRFAM: FolC bifunctional protein; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: gtn:GTNG_2566 folylpolyglutamate synthase. | 0.447 |
ACS23599.1 | folD | GWCH70_0708 | GWCH70_2324 | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | Methylenetetrahydrofolate dehydrogenase (NADP(+)); Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.951 |
ACS23599.1 | gcvT | GWCH70_0708 | GWCH70_2361 | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | Glycine cleavage system T protein; The glycine cleavage system catalyzes the degradation of glycine. | 0.961 |
ACS23599.1 | glyA | GWCH70_0708 | GWCH70_3313 | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.960 |
ACS23599.1 | thyA | GWCH70_0708 | GWCH70_1581 | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.945 |
ACS24377.1 | ACS23599.1 | GWCH70_1582 | GWCH70_0708 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | 0.937 |
ACS24377.1 | ACS25267.1 | GWCH70_1582 | GWCH70_2572 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | TIGRFAM: FolC bifunctional protein; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: gtn:GTNG_2566 folylpolyglutamate synthase. | 0.923 |
ACS24377.1 | gcvT | GWCH70_1582 | GWCH70_2361 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Glycine cleavage system T protein; The glycine cleavage system catalyzes the degradation of glycine. | 0.921 |
ACS24377.1 | glyA | GWCH70_1582 | GWCH70_3313 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.937 |
ACS24377.1 | tdk | GWCH70_1582 | GWCH70_3326 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | PFAM: thymidine kinase; KEGG: gtn:GTNG_3325 thymidine kinase. | 0.419 |
ACS24377.1 | thyA | GWCH70_1582 | GWCH70_1581 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.999 |
ACS25146.1 | ACS25591.1 | GWCH70_2450 | GWCH70_2912 | TIGRFAM: ComE operon protein 2; PFAM: CMP/dCMP deaminase zinc-binding; KEGG: gka:GK2516 late competence protein (DNA binding and uptake). | PFAM: metallophosphoesterase; 5'-Nucleotidase domain protein; KEGG: gka:GK2983 hypothetical protein; Belongs to the 5'-nucleotidase family. | 0.905 |
ACS25146.1 | tdk | GWCH70_2450 | GWCH70_3326 | TIGRFAM: ComE operon protein 2; PFAM: CMP/dCMP deaminase zinc-binding; KEGG: gka:GK2516 late competence protein (DNA binding and uptake). | PFAM: thymidine kinase; KEGG: gtn:GTNG_3325 thymidine kinase. | 0.932 |
ACS25146.1 | thyA | GWCH70_2450 | GWCH70_1581 | TIGRFAM: ComE operon protein 2; PFAM: CMP/dCMP deaminase zinc-binding; KEGG: gka:GK2516 late competence protein (DNA binding and uptake). | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. | 0.938 |
ACS25146.1 | tmk | GWCH70_2450 | GWCH70_0026 | TIGRFAM: ComE operon protein 2; PFAM: CMP/dCMP deaminase zinc-binding; KEGG: gka:GK2516 late competence protein (DNA binding and uptake). | Thymidylate kinase; Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis; Belongs to the thymidylate kinase family. | 0.930 |
ACS25267.1 | ACS23599.1 | GWCH70_2572 | GWCH70_0708 | TIGRFAM: FolC bifunctional protein; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: gtn:GTNG_2566 folylpolyglutamate synthase. | PFAM: homocysteine S-methyltransferase; methylenetetrahydrofolate reductase; KEGG: gka:GK0717 bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein. | 0.447 |
ACS25267.1 | ACS24377.1 | GWCH70_2572 | GWCH70_1582 | TIGRFAM: FolC bifunctional protein; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: gtn:GTNG_2566 folylpolyglutamate synthase. | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. | 0.923 |
ACS25267.1 | folD | GWCH70_2572 | GWCH70_2324 | TIGRFAM: FolC bifunctional protein; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: gtn:GTNG_2566 folylpolyglutamate synthase. | Methylenetetrahydrofolate dehydrogenase (NADP(+)); Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. | 0.582 |
ACS25267.1 | glyA | GWCH70_2572 | GWCH70_3313 | TIGRFAM: FolC bifunctional protein; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: gtn:GTNG_2566 folylpolyglutamate synthase. | Glycine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. | 0.714 |