node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
cyaY | fdx | b3807 | b2525 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | 0.960 |
cyaY | hscA | b3807 | b2526 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Dnak-like molecular chaperone specific for iscu; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU | 0.939 |
cyaY | hscB | b3807 | b2527 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Hsca co-chaperone, j domain-containing protein hsc56; Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA | 0.975 |
cyaY | iscA | b3807 | b2528 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Iron-sulfur cluster insertion protein isca; Is able to transfer iron-sulfur clusters to apo-ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that IscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transient iron-sulfur cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin reductase system TrxA/TrxB | 0.632 |
cyaY | iscR | b3807 | b2531 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Rrf2 family transcriptional regulator, iron-sulfur cluster assembly transcription factor; Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA | 0.604 |
cyaY | iscS | b3807 | b2530 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Cysteine desulfurase (trna sulfurtransferase), plp-dependent; Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Preferentially binds to disordered IscU on which the Fe-S is assembled, IscU converts to the structured state and then dissociates from IscS to transfer the Fe-S to a [...] | 0.997 |
cyaY | iscU | b3807 | b2529 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Nitrogen fixation protein nifu and related proteins; A scaffold on which IscS assembles Fe-S clusters. Exists as 2 interconverting forms, a structured (S) and disordered (D) form. The D- state is the preferred substrate for IscS. Converts to the S-state when an Fe-S cluster is assembled, which helps it dissociate from IscS to transfer the Fe-S to an acceptor. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters | 0.999 |
cyaY | iscX | b3807 | b2524 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Fe(2+) donor and activity modulator for cysteine desulfurase; May function as iron donor in the assembly of iron-sulfur clusters | 0.904 |
cyaY | nfuA | b3807 | b3414 | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | Fe/s biogenesis protein, putative scaffold/chaperone protein; Involved in iron-sulfur cluster biogenesis under severe conditions such as iron starvation or oxidative stress. Binds a 4Fe-4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins. Required for E.coli to sustain oxidative stress and iron starvation. Also necessary for the use of extracellular DNA as the sole source of carbon and energy | 0.585 |
fdx | cyaY | b2525 | b3807 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | 0.960 |
fdx | hscA | b2525 | b2526 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Dnak-like molecular chaperone specific for iscu; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU | 0.999 |
fdx | hscB | b2525 | b2527 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Hsca co-chaperone, j domain-containing protein hsc56; Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA | 0.999 |
fdx | iscA | b2525 | b2528 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Iron-sulfur cluster insertion protein isca; Is able to transfer iron-sulfur clusters to apo-ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that IscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transient iron-sulfur cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin reductase system TrxA/TrxB | 0.985 |
fdx | iscR | b2525 | b2531 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Rrf2 family transcriptional regulator, iron-sulfur cluster assembly transcription factor; Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA | 0.967 |
fdx | iscS | b2525 | b2530 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Cysteine desulfurase (trna sulfurtransferase), plp-dependent; Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Preferentially binds to disordered IscU on which the Fe-S is assembled, IscU converts to the structured state and then dissociates from IscS to transfer the Fe-S to a [...] | 0.997 |
fdx | iscU | b2525 | b2529 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Nitrogen fixation protein nifu and related proteins; A scaffold on which IscS assembles Fe-S clusters. Exists as 2 interconverting forms, a structured (S) and disordered (D) form. The D- state is the preferred substrate for IscS. Converts to the S-state when an Fe-S cluster is assembled, which helps it dissociate from IscS to transfer the Fe-S to an acceptor. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters | 0.999 |
fdx | iscX | b2525 | b2524 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Fe(2+) donor and activity modulator for cysteine desulfurase; May function as iron donor in the assembly of iron-sulfur clusters | 0.999 |
fdx | nfuA | b2525 | b3414 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Fe/s biogenesis protein, putative scaffold/chaperone protein; Involved in iron-sulfur cluster biogenesis under severe conditions such as iron starvation or oxidative stress. Binds a 4Fe-4S cluster, can transfer this cluster to apoproteins, and thereby intervenes in the maturation of Fe/S proteins. Could also act as a scaffold/chaperone for damaged Fe/S proteins. Required for E.coli to sustain oxidative stress and iron starvation. Also necessary for the use of extracellular DNA as the sole source of carbon and energy | 0.844 |
fdx | sufS | b2525 | b1680 | [2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins | Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L- selenocysteine. Selenocysteine lyase activity is however unsure in vivo | 0.731 |
hscA | cyaY | b2526 | b3807 | Dnak-like molecular chaperone specific for iscu; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU | Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly . May act as a regulator of Fe-S biogenesis Can bind both Fe(2+) and Fe(3+) ions . In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions . In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU . In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU | 0.939 |