Rv1090, (MTV017.43), len: 151 aa. Probable celA2b,second part of cellulase (endoglucanase), similar to C-terminus of others e.g. O08468 cellulase CEL2 from Streptomyces halstedi (377 aa), FASTA scores: opt: 554,E(): 1.2e-30, (52.0% identity in 152 aa overlap); etc. Gene appears to have been inactivated by frameshift mutations but no errors could be found that would account for this. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007); Belongs to the glycosyl hydrolase 12 (cellulase H) family.

Rv0315, (MTCY63.20), len: 294 aa. Possible beta-1,3-glucanase precursor (has hydrophobic stretch in its N-terminal part), similar to others e.g. Q51333|AAC44371.1 beta-1,3-glucanase II a from Oerskovia xanthineolytica (306 aa), FASTA scores: opt: 76, E(): 3e-14, (34.1% identity in 302 aa overlap); and AAC38290.1|AF052745 beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa). Contains glycosyl hydrolases family 16 active site signature (PS01034).

Rv1089A, len: 34 aa. Probable celA2a, first part of cellulase (endoglucanase), similar to N-terminus of others. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).

Probable glucanase GlgE; Essential maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)- glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP5 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Exhibits an alpha-retaining catalytic mechanism. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen in the presence [...]

Rv3096, (MTCY164.07), len: 379 aa. Hypothetical protein, with slight similarity to several proteins e.g. Q09671|OYEB_SCHPO|SPAC5H10.10 putative NADPH dehydrogenase C5H10.10 (old yellow enzyme homolog) from Schizosaccharomyces pombe (Fission yeast) (392 aa), FASTA scores: opt: 125, E(): 1.1, (25.45% identity in 165 aa overlap); and Q12603|XYNA_DICTH beta-1,4-xylanase (endo-1,4-beta-xylanase) from Dictyoglomus thermophilum (352 aa), FASTA scores: opt: 124, E(): 1.2, (25.65% identity in 195 aa overlap); etc. Contains glycosyl hydrolases family 5 signature (PS00659). Predicted to be an out [...]

Alpha (1->4) glucosyltransferase; Glucosyltransferase that uses UDP-glucose as the sugar donor to elongate alpha-(1->4)-glucans. Is involved in the biosynthesis of both 6-O-methylglucosyl lipopolysaccharides (MGLP) and glycogen. May also use ADP-glucose as substrate.

Possible enoyl-CoA hydratase EchA4 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv0673, (MTCI376.01c, MTV040.01), len: 312 aa. Possible echA4, enoyl-CoA hydratase, showing similarity with others e.g. NP_419216.1|NC_002696 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus (256 aa); Q52995|ECHH_RHIME probable enoyl-CoA hydratase from Sinorhizobium meliloti (257 aa), FASTA scores: opt: 210, E(): 1.2e-06, (27.9% identity in 280 aa overlap); etc. Also similar to other enoyl-CoA hydratases from Mycobacterium tuberculosis e.g. P95279|MTCY09F9.29|ECHA13| [...]

Rv0672, (MTCI376.02c), len: 542 aa. Probable fadE8,acyl-CoA dehydrogenase, highly similar to many e.g. CAC33951.1|AL589708 putative acyl-CoA dehydrogenase from Streptomyces coelicolor (557 aa); P33224|AIDB_ECOLI|B4187 aidb protein (acyl-CoA dehydrogenases family) from Escherichia coli strain K12 (546 aa), FASTA scores: opt: 1369, E(): 0, (44.1% identity in 524 aa overlap); etc. Also similar to several other M. tuberculosis proteins e.g. Rv0154c|MTCI5.28c FASTA score: (26.3% identity in 342 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 2 (PS00073). Belongs to the acyl-CoA [...]

Possible exported protein; GDSL lipase that catalyzes the hydrolysis of p-nitrophenyl (pNP) esters. pNP-decanoate (C10) is the preferred substrate. It can also use pNP-octanoate (C8), pNP-dodecanoate (C12) and pNP- tetradecanoate (C14). Has lower activity with pNP-butyrate (C4), pNP- palmitate (C16) and pNP-stearate (C18). Does not show phospholipase A1 activity. Might help bacteria to utilize available lipids for its growth as well as provide resistance to various intracellular stresses by cell wall modulation resulting in enhanced intracellular survival.