Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III); May function to modify mycofactocin, a conserved polypeptide that might serve as an electron carrier. The genes for mycofactocin and other proteins proposed to function in its maturation are found in a conserved gene cluster; Belongs to the radical SAM superfamily.

Conserved hypothetical protein; May play a role in the maturation of mycofactocin, a conserved polypeptide that might serve as an electron carrier. The genes for mycofactocin and other proteins proposed to function in its maturation are found in a conserved gene cluster.

Probable membrane sugar transferase; May play a role in the maturation of mycofactocin, a conserved polypeptide that might serve as an electron carrier. The genes for mycofactocin and other proteins proposed to function in its maturation are found in a conserved gene cluster; Belongs to the glycosyltransferase 2 family.

Mycofactocin precursor protein; A putative polypeptide that may function as an electron carrier; the C-terminal 8 residues are highly conserved. The genes for mycofactocin and other proteins proposed to function in its maturation are found in a conserved gene cluster.

Rv1617, (MTCY01B2.09), len: 472 aa. Probable pykA,pyruvate kinase. FASTA best: Q46078 pyruvate kinase from corynebacterium glutamicum (475 aa), opt: 2221, E(): 0,(72.2% identity in 468 aa overlap). Belongs to the pyruvate kinase family. Phosphorylated in vitro by PknJ|Rv2088 (See Arora et al., 2010).

Rv1872c, (MTCY180.46, MTCY359.01), len: 414 aa (start uncertain). Possible lldD2, L-lactate dehydrogenase (cytochrome), similar to other lactate dehydrogenases and other oxidases e.g. LLDD_ECOLI|P33232 l-lactate dehydrogenase (cytochrome) from Escherichia coli strain K12 (396 aa), FASTA results: opt: 674, E(): 1.1e-37, (40.5% identity in 279 aa overlap); Q51135 lactate dehydrogenase from Neisseria meningitidis (390 aa), FASTA results: opt: 309, E(): 4.1e-15, (42.5% identity in 113 aa overlap); etc. Also shows similarity with Rv0694|lldD1|MTCY210.11 possible L-lactate dehydrogenase (cyt [...]

Probable oxidoreductase (alpha subunit); Component of KG oxidoreductase (KOR) that catalyzes the CoA- dependent oxidative decarboxylation of 2-oxoglutarate (alpha- ketoglutarate, KG) to succinyl-CoA. Methyl viologen can act as electron acceptor in vitro; the physiologic electron acceptor is unknown. Is involved in the alternative TCA pathway that functions concurrently with fatty acid beta-oxidation. Since a growing body of evidence indicates that lipids (for example cholesterol and fatty acids) are a predominant growth substrate for M.tuberculosis during infection, flux through KOR li [...]

Putative malate oxidoreductase [NAD]; Rv2332, (MTCY3G12.02c, MTCY98.01, MT2394), len: 548 aa. Probable mez, malate oxidoreductase [NAD] dependent (malic enzyme), highly similar to others e.g. O34389|MALS putative malolactic enzyme [includes: malic enzyme; L-lactate dehydrogenase] from Bacillus subtilis (566 aa),FASTA scores: opt: 1927, E(): 5.5e-111, (52.9% identity in 539 aa overlap); P45868|MAO2_BACSU|YWKA probable NAD-dependent malic enzyme from Bacillus subtilis (582 aa),FASTA scores: opt: 1849, E(): 3.6e-106, (50.45% identity in 543 aa overlap); Q48796|MLES_OENOE malolactic enzyme [...]

Probable pyruvate carboxylase Pca (pyruvic carboxylase); Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.