Polyphosphate kinase PPK (polyphosphoric acid kinase) (ATP-polyphosphate phosphotransferase); Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP); Belongs to the polyphosphate kinase 1 (PPK1) family.

Conserved hypothetical protein; Degradation of inorganic polyphosphates (polyP). Releases orthophosphate processively from the ends of the polyP chain. Prefers short-chain length polyphosphates as substrates. Can also hydrolyze ATP and ADP substrates, but lacks GTPase activity. Cannot hydrolyze pppGpp to ppGpp; Belongs to the GppA/Ppx family.

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp, which is then hydrolyzed to form ppGpp, as well as the hydrolysis of ppGpp. RelA is probably a key factor in the pathogenesis of M.tuberculosis as it regulates the intracellular concentrations of (p)ppGpp.

Polyphosphate kinase Ppk2 (polyphosphoric acid kinase); Uses inorganic polyphosphate (polyP) as a donor to convert GDP to GTP. In addition, modulates nucleotide triphosphate synthesis catalyzed by the nucleoside diphosphate kinase (Ndk) in favor of GTP production over CTP or UTP. Plays an important role in survival of M.tuberculosis in macrophages.

Rv1024, (MTCY10G2.25c), len: 228 aa. Possible conserved membrane protein, with a hydrophobic region from aa 83-101. Equivalent to ML0256|NP_301311.1|NC_002677 possible conserved membrane protein from Mycobacterium leprae (227 aa), S&W scores: 178, E()= 2e-72, Identities: 145/203 (71%). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).

Highly conserved protein; Rv2603c, (MTCI270A.02), len: 251 aa. Highly conserved protein, equivalent to Q49645|YQ03_MYCLE|ML0475|U1177B|B1177_C2_181 hypothetical 26.6 KDA protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1514, E(): 2.2e-84, (92.45% identity in 251 aa overlap). Also highly similar to Q9L288|SCL2.11c hypothetical 26.8 KDA protein from Streptomyces coelicolor (250 aa), FASTA scores: opt: 1268, E(): 1.5e-69, (76.7% identity in 249 aa overlap); Q9AE12|YFCA hypothetical structural protein from Corynebacterium glutamicum (Brevibacterium flavum) (251 aa), FASTA sco [...]

Probable enolase Eno; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis; Belongs to the enolase family.

Conserved protein; Rv3231c, (MTCY20B11.06c), len: 169 aa. Conserved protein, similar to Q9KYX9|SCE33.03c hypothetical 17.4 KDA protein from Streptomyces coelicolor (167 aa), FASTA scores: opt: 415, E(): 6.6e-19, (49.1% identity in 171 aa overlap).

Rv1022, (MTCY10G2.27c), len: 243 aa. Probable lpqU conserved lipoprotein. Similar to Mycobacterium tuberculosis hypothetical protein Rv1230c|MTV006.02C, FASTA scores: E(): 2.8e-18, (37.9% identity in 240 aa overlap). Similar to AL133423|SC4A7.37 hypothetical protein from Streptomyces coelicolor (421 aa), FASTA scores: opt: 474,E(): 2.7e-21, (42.2% identity in 211 aa overlap). Contains PS00013 Prokaryotic membrane lipoprotein lipid attachment site.