node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
Rv1496 | mazE4 | Rv1496 | Rv1494 | Possible transport system kinase; Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable); Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily. | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | 0.803 |
Rv1496 | mazF4 | Rv1496 | Rv1495 | Possible transport system kinase; Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable); Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily. | Possible toxin MazF4; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an endoribonuclease (mRNA interferase) on single-strand mRNA, cleaving between the first and second bases in the sequence UCGCU. Overexpression in M.smegmatis but not E.coli inhibits growth, this effect is neutralized by coexpression with cognate toxin MazE4. Belongs to the PemK/MazF family. | 0.803 |
Rv1496 | mutA | Rv1496 | Rv1492 | Possible transport system kinase; Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable); Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily. | Probable methylmalonyl-CoA mutase small subunit MutA (MCM); Catalyzes the isomerization of succinyl-CoA to methylmalonyl- CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates; Belongs to the methylmalonyl-CoA mutase family. | 0.982 |
Rv1496 | mutB | Rv1496 | Rv1493 | Possible transport system kinase; Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable); Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily. | Probable methylmalonyl-CoA mutase large subunit MutB (MCM); Catalyzes the isomerization of succinyl-CoA to methylmalonyl- CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. | 0.996 |
Rv3387 | mazF4 | Rv3387 | Rv1495 | Possible transposase; Rv3387, (MTV004.45), len: 225 aa. Possible transposase, showing very weak similarity to other is element proteins, and similar to various hypothetical proteins. | Possible toxin MazF4; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an endoribonuclease (mRNA interferase) on single-strand mRNA, cleaving between the first and second bases in the sequence UCGCU. Overexpression in M.smegmatis but not E.coli inhibits growth, this effect is neutralized by coexpression with cognate toxin MazE4. Belongs to the PemK/MazF family. | 0.629 |
fadD25 | mazE4 | Rv1521 | Rv1494 | Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25,fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | 0.803 |
fadD25 | mazF4 | Rv1521 | Rv1495 | Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25,fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. | Possible toxin MazF4; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an endoribonuclease (mRNA interferase) on single-strand mRNA, cleaving between the first and second bases in the sequence UCGCU. Overexpression in M.smegmatis but not E.coli inhibits growth, this effect is neutralized by coexpression with cognate toxin MazE4. Belongs to the PemK/MazF family. | 0.720 |
fadD25 | mutA | Rv1521 | Rv1492 | Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25,fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. | Probable methylmalonyl-CoA mutase small subunit MutA (MCM); Catalyzes the isomerization of succinyl-CoA to methylmalonyl- CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates; Belongs to the methylmalonyl-CoA mutase family. | 0.513 |
fadD25 | mutB | Rv1521 | Rv1493 | Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25,fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. | Probable methylmalonyl-CoA mutase large subunit MutB (MCM); Catalyzes the isomerization of succinyl-CoA to methylmalonyl- CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. | 0.655 |
mazE4 | Rv1496 | Rv1494 | Rv1496 | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | Possible transport system kinase; Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable); Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily. | 0.803 |
mazE4 | fadD25 | Rv1494 | Rv1521 | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase); Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25,fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. | 0.803 |
mazE4 | mazE6 | Rv1494 | Rv1991A | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | Antitoxin MazE6; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis counteracts the ribonuclease activity of cognate toxin MazF6. | 0.542 |
mazE4 | mazF4 | Rv1494 | Rv1495 | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | Possible toxin MazF4; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an endoribonuclease (mRNA interferase) on single-strand mRNA, cleaving between the first and second bases in the sequence UCGCU. Overexpression in M.smegmatis but not E.coli inhibits growth, this effect is neutralized by coexpression with cognate toxin MazE4. Belongs to the PemK/MazF family. | 0.999 |
mazE4 | mazF6 | Rv1494 | Rv1991c | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | Toxin MazF6; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU. Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'- hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli [...] | 0.477 |
mazE4 | mutA | Rv1494 | Rv1492 | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | Probable methylmalonyl-CoA mutase small subunit MutA (MCM); Catalyzes the isomerization of succinyl-CoA to methylmalonyl- CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates; Belongs to the methylmalonyl-CoA mutase family. | 0.775 |
mazE4 | mutB | Rv1494 | Rv1493 | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | Probable methylmalonyl-CoA mutase large subunit MutB (MCM); Catalyzes the isomerization of succinyl-CoA to methylmalonyl- CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. | 0.840 |
mazE6 | mazE4 | Rv1991A | Rv1494 | Antitoxin MazE6; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis counteracts the ribonuclease activity of cognate toxin MazF6. | Possible antitoxin MazE4; Antitoxin component of a type II toxin-antitoxin (TA) system. In M.smegmatis neutralizes the toxic effect of mRNA interferase MazF4, its cognate toxin, on growth. | 0.542 |
mazE6 | mazF3 | Rv1991A | Rv1102c | Antitoxin MazE6; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis counteracts the ribonuclease activity of cognate toxin MazF6. | Toxin MazF3; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an mRNA and 23S rRNA interferase, cleaving predominantly after the first 2 Us in the sequence 5'-UUCCU-3'; in 23S rRNA only cleaves once in the ribosomal A site in dissociated but not intact ribosomes. Cleavage of 23S rRNA inhibits protein translation; the 23S rRNA region cleaved is involved in tRNA-binding in the A site, 30S and 50S subunit interaction and ribosome recycling factor association. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. It dramatically increases per [...] | 0.782 |
mazE6 | mazF4 | Rv1991A | Rv1495 | Antitoxin MazE6; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis counteracts the ribonuclease activity of cognate toxin MazF6. | Possible toxin MazF4; Toxic component of a type II toxin-antitoxin (TA) system. Acts as an endoribonuclease (mRNA interferase) on single-strand mRNA, cleaving between the first and second bases in the sequence UCGCU. Overexpression in M.smegmatis but not E.coli inhibits growth, this effect is neutralized by coexpression with cognate toxin MazE4. Belongs to the PemK/MazF family. | 0.638 |
mazE6 | mazF6 | Rv1991A | Rv1991c | Antitoxin MazE6; Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis counteracts the ribonuclease activity of cognate toxin MazF6. | Toxin MazF6; Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU. Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'- hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli [...] | 0.990 |