Probable lyase; Rv3684, (MTV025.032), len: 346 aa. Probable lyase, and more specifically a cysteine synthase, highly similar to many lyases e.g. Q9K3N2|SCG20A.08c putative lyase from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1469,E(): 3.7e-85, (63.35% identity in 341 aa overlap) (shorter 31 aa at N-terminus); Q9KT44|VC1061 cysteine synthase/ cystathionine beta-synthase family protein from Vibrio cholerae (355 aa), FASTA scores: opt: 1366, E(): 1.1e-78,(63.25% identity in 321 aa overlap); Q9I4R3|PA1061 hypothetical protein from Pseudomonas aeruginosa (365 aa),FASTA scores: op [...]

Probable cystathionine beta-synthase Cbs (serine sulfhydrase) (beta-thionase) (hemoprotein H-450); Rv1077, (MTV017.30), len: 464 aa. Probable cbs (previously cysM2), cystathionine beta-synthase, similar throughout its length to many eukaryotic cystathionine beta-synthases e.g. P32232|CBS_RAT cystathionine beta-synthase (560 aa), FASTA scores: opt: 951, E(): 0,(40.2% identity in 450 aa overlap); also similar in N-terminal domain (aa 1 - 330) to Rv2334|MTCY98.03 CysK Mycobacterium tuberculosis (310 aa), FASTA scores: opt: 855, E(): 0, (46.8% identity in 314 overlap); and other cysteine s [...]

Rv0069c, (MTV030.12c), len: 461 aa. Probable sdaA,L-serine dehydratase. Belongs to the iron-sulfur dependent L-serine dehydratase family. Cofactor: iron-sulfur (4FE-4S) (probable).

O-phosphoserine sulfhydrylase; Catalyzes the formation of a covalent CysO-cysteine adduct via a sulfur transfer, using the thiocarboxylated sulfur carrier protein CysO-COSH as sulfur donor and O-phospho-L-serine (OPS) as sulfur acceptor. Can also use sodium sulfide as sulfur donor in vitro, albeit with less efficiency, but not thiosulfate or thio-nitro- benzoate. O-acetylserine (OAS) is a very poor substrate in comparison with OPS. May be of particular importance for cysteine biosynthesis in the persistent phase of M.tuberculosis; Belongs to the cysteine synthase/cystathionine beta- sy [...]

Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase); Rv3580c, (MTCY06G11.27c), len: 469 aa. Probable cysS1, cysteinyl-tRNA synthetase, equivalent to P57990|SYC1_MYCLE|CYSS1|CYSS|ML0323 cysteinyl-tRNA synthetase 1 from Mycobacterium leprae (473 aa) FASTA scores: opt: 2825, E(): 3.4e-172, (86.5% identity in 467 aa overlap). Also similar to many e.g. Q9L0Q6|SCD8A.08 from Streptomyces coelicolor (613 aa), FASTA scores: opt: 1834,E(): 4.7e-109, (57.5% identity in 461 aa overlap); Q9I2U7|CYSS|PA1795 from Pseudomonas aeruginosa (460 aa) FASTA scores: opt [...]

Probable phosphoserine phosphatase SerB2 (PSP) (O-phosphoserine phosphohydrolase) (pspase); Catalyzes the dephosphorylation of O-phospho-L-serine into L- serine, a step in the L-serine biosynthetic pathway. Exhibits high specificity for L-phosphoserine compared to substrates like L-phosphothreonine (5% relative activity) and L-phosphotyrosine (1.7% relative activity). Belongs to the HAD-like hydrolase superfamily. SerB family.

Serine hydroxymethyltransferase 1 GlyA1; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L- allo-threonine; Belongs to the SHMT family.

Serine hydroxymethyltransferase GlyA2 (serine methylase 2) (SHMT 2); Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L- allo-threonine; Belongs to the SHMT family.

S-sulfocysteine synthase; Catalyzes the synthesis of S-sulfocysteine, utilizing O- phosphoserine (OPS) and thiosulfate as substrates. To a lesser extent, can also use sulfide as donor substrate, producing L-cysteine. CysK2 thus provides a third metabolic route to cysteine, either directly using sulfide as donor or indirectly via S-sulfocysteine. S- sulfocysteine might also act as a signaling molecule triggering additional responses in redox defense in the pathogen upon exposure to reactive oxygen species during intracellular survival or dormancy. Cannot utilize thiocarboxylated CysO as [...]