Isochorismate synthase MbtI; Involved in the incorporation of salicylate into the virulence-conferring salicylate-based siderophore mycobactin. Catalyzes the initial conversion of chorismate to yield the intermediate isochorismate (isochorismate synthase activity), and the subsequent elimination of the enolpyruvyl side chain to give salicylate (isochorismate pyruvate-lyase activity). In the absence of magnesium, MbtI displays a chorismate mutase activity and converts chorismate to prephenate.

Rv2385, (MTCY253.36c), len: 306 aa. Putative mbtJ,acetyl hydrolase (see citations below), showing some similarity with various hydrolases including acetyl hydrolases e.g. Q9ZBM4|MLCB1450.08|ML0314 putative hydrolase/esterase from Mycobacterium leprae (335 aa),FASTA scores: opt: 449, E(): 6.7e-21, (33.85% identity in 313 aa overlap); AAK47950|MT3591 Esterase from M. tuberculosis strain CDC1551 (327 aa), FASTA scores: opt: 469, E(): 3.6e-22, (35% identity in 283 aa overlap); Q9X8J4|SCE9.22 putative esterase from Streptomyces coelicolor (266 aa), FASTA scores: opt: 430,E(): 8.5e-20,(38% i [...]

Polyketide synthetase MbtC (polyketide synthase); Rv2382c, (MTCY22H8.03), len: 444 aa. MbtC,polyketide synthase (see citations below), similar in part to several synthases e.g. Q9F7T9 avermectin polyketide synthase (fragment) from Streptomyces avermitilis (3626 aa), FASTA scores: opt: 1458, E(): 7e-82, (50.65% identity in 446 aa overlap); AAG23264|SPNA polyketide synthase loading and extender module 1 from Saccharopolyspora spinosa (2595 aa) FASTA scores: opt: 1441, E(): 6e-81,(49.1% identity in 446 aa overlap); O33954|TYLG tylactone synthase starter module and modules 1 & 2 from Strep [...]

Putative conserved protein MbtH; Could be involved in mycobactin synthesis.

Polyketide synthase Pks13; Rv3800c, (MTV026.05c), len: 1733 aa. Probable pks13,polyketide synthase, equivalent to Q9CDB1|PKS13|ML0101 polyketide synthase from Mycobacterium leprae (1784 aa),FASTA scores: opt: 7454, E(): 0, (83.6% identity in 1748 aa overlap); and similar to Q9Z5K6|ML2357|MLCB12.02c putative polyketide synthase from Mycobacterium leprae (1871 aa),FASTA scores: opt: 1682, E(): 1.2e-85, (38.3% identity in 1096 aa overlap). Also similar in part to many e.g. Q9ADL6|SORA soraphen polyketide synthase a from Polyangium cellulosum (6315 aa) FASTA scores: opt: 1422, E(): 1e-70,( [...]

Iron-regulated transporter IrtA; Part of the ABC transporter complex IrtAB involved in iron import. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation. Required for replication in human macrophages and in mouse lungs.

Lysine-N-oxygenase MbtG (L-lysine 6-monooxygenase) (lysine N6-hydroxylase); Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro.

Probable isochorismate synthase EntC (isochorismate hydroxymutase) (enterochelin biosynthesis); Catalyzes the conversion of chorismate to isochorismate.

Rv2380c, (MTCY22H8.05), len: 1682 aa. MbtE, peptide synthetase (see citations below), similar in part to several synthases e.g. O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 2635, E(): 1.9e-146, (36.8% identity in 1657 aa overlap); O05647|SNBDE virginiamycin S synthetase (fragment) from Streptomyces virginiae (1997 aa) FASTA scores: opt: 2580, E(): 1.6e-143, (40.65% identity in 1163 aa overlap); Q9R9I2|DHBF protein involved in siderophore production from Bacillus subtilis (2378 aa),FASTA scores: opt: 2388, E(): 3.6e-132, [...]