node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
Rv3521 | cyp142 | Rv3521 | Rv3518c | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Probable cytochrome P450 monooxygenase 142 Cyp142; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4- en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate. In vitro, Cyp142 catalyzes with equal preference the oxidation of both (25R)- and (25S)-26- hydroxycholest-4-en-3-one diastereomers to the corresponding carboxy [...] | 0.614 |
Rv3521 | echA19 | Rv3521 | Rv3516 | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv3516, (MTV023.23), len: 263 aa. Possible echA19,enoyl-CoA hydratase, similar to other e.g. Q9ZHG2|ECHA1 from Rhodococcus fascians (275 aa) FASTA scores: opt: 613,E(): 6.4e-32, (45.15% identity in 259 aa overlap); P76082|PAAF_ECOLI|B1393 from Escherichia coli strain K12 (255 aa), FASTA scores: opt: 523, E(): 3.3e-26, (33.6% identity in 256 aa overlap); Q9I393|PA1629 from Pseudomonas aeruginosa (261 aa), FASTA scores: opt: 475, E(): 3.8e-23,(36.85% identity in 247 aa overlap); etc. Also si [...] | 0.881 |
Rv3521 | fadA5 | Rv3521 | Rv3546 | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Probable acetyl-CoA acetyltransferase FadA5 (acetoacetyl-CoA thiolase); Involved in the beta-oxidation of the cholesterol side chain. It is important for utilization of cholesterol as a sole carbon source in vitro and for full virulence in the chronic stage of mouse lung infection. Catalyzes the thiolysis of 3,22-dioxochol-4-en-24-oyl-CoA to yield 3-oxo-4-pregnene-20-carboxyl- CoA (3-OPC-CoA) and acetyl-CoA. Also able to use acetoacetyl-CoA (AcAcCoA) as substrate. | 0.835 |
Rv3521 | fadD19 | Rv3521 | Rv3515c | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase); Catalyzes the activation of long-chain fatty acids as acyl- coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension. Also involved in the degradation of cholesterol via the degradation of the side chains of C-24 branched-chain sterols. Catalyzes the ATP-dependent CoA thioesterification of the sterol 3- oxocholest-4-en-26-oate to yield 3-oxocholest-4-en-26-oyl-CoA. It can also use 3beta-hydroxy-5-cholesten-26-oate. | 0.878 |
Rv3521 | fadE26 | Rv3521 | Rv3504 | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Probable acyl-CoA dehydrogenase FadE26; Involved in the first cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of acyl-CoA ester side chains of (25S)-3-oxo-cholest-4-en-26-oyl-CoA (3-OCS-CoA) to yield (24E)-3-oxo-cholest-4,24-dien-26-oyl-CoA. Also able to dehydrogenate steroyl-CoA such as 3-oxo- chol-4-en-24-oyl-CoA (3-OCO-CoA) as well as 3-oxo-4-pregnene-20- carboxyl-CoA (3-OPC-CoA). It dehydrogenates only (25S)-OCS-CoA diastereomer ( [...] | 0.754 |
Rv3521 | fadE27 | Rv3521 | Rv3505 | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Probable acyl-CoA dehydrogenase FadE27; Involved in the first cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of acyl-CoA ester side chains of (25S)-3-oxo-cholest-4-en-26-oyl-CoA (3-OCS-CoA) to yield (24E)-3-oxo-cholest-4,24-dien-26-oyl-CoA. Also able to dehydrogenate steroyl-CoA such as 3-oxo- chol-4-en-24-oyl-CoA (3-OCO-CoA) as well as 3-oxo-4-pregnene-20- carboxyl-CoA (3-OPC-CoA). It dehydrogenates only (25S)-OCS-CoA diastereomer. | 0.509 |
Rv3521 | fadE34 | Rv3521 | Rv3573c | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Probable acyl-CoA dehydrogenase FadE34; Involved in the second cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the highly specific dehydrogenation of acyl-CoA ester side chains of 3- oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA) to yield 3-oxochol-4,22-dien-24- oyl-CoA. | 0.732 |
Rv3521 | ltp3 | Rv3521 | Rv3523 | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Rv3523, (MTCY03C7.33c), len: 394 aa. Probable ltp3,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30037|AF0202 3-ketoacyl-CoA thiolase (ACAB-6) from Archaeoglobus fulgidus (380 aa) FASTA scores: opt: 782, E(): 1.7e-40, (38.35% identity in 386 aa overlap); Q9Y9A1|APE2384 long hypothetical non specific lipid-transfer protein (acethyl CoA synthetase) from Aeropyrum pernix (394 aa), FASTA scores: opt: 626, E(): 5.9e-31, (35.75% identity in 386 aa overlap); BAB59210|TVG0067506 lipid transfer protein from Thermoplasma volcanium (390 aa), FASTA scores: opt: 591,E(): [...] | 0.996 |
Rv3521 | ltp4 | Rv3521 | Rv3522 | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | Rv3522, (MTV023.29), len: 354 aa. Possible ltp4,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30103|AF0134 3-ketoacyl-CoA thiolase (ACAB-4) from Archaeoglobus fulgidus (398 aa) FASTA scores: opt: 352, E(): 5.3e-15, (30.45% identity in 381 aa overlap); O29295|AF0967 3-ketoacyl-CoA thiolase (ACAB-9) from Archaeoglobus fulgidus (400 aa) FASTA scores: opt: 312,E(): 1.8e-12, (28.05% identity in 367 aa overlap); O29294|AF0968 3-ketoacyl-CoA thiolase (ACAB-10) from Archaeoglobus fulgidus (388 aa), FASTA scores: opt: 293,E(): 2.9e-11, (25.9% identity in 309 aa overl [...] | 0.996 |
cyp125 | cyp142 | Rv3545c | Rv3518c | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Probable cytochrome P450 monooxygenase 142 Cyp142; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4- en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate. In vitro, Cyp142 catalyzes with equal preference the oxidation of both (25R)- and (25S)-26- hydroxycholest-4-en-3-one diastereomers to the corresponding carboxy [...] | 0.943 |
cyp125 | echA19 | Rv3545c | Rv3516 | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv3516, (MTV023.23), len: 263 aa. Possible echA19,enoyl-CoA hydratase, similar to other e.g. Q9ZHG2|ECHA1 from Rhodococcus fascians (275 aa) FASTA scores: opt: 613,E(): 6.4e-32, (45.15% identity in 259 aa overlap); P76082|PAAF_ECOLI|B1393 from Escherichia coli strain K12 (255 aa), FASTA scores: opt: 523, E(): 3.3e-26, (33.6% identity in 256 aa overlap); Q9I393|PA1629 from Pseudomonas aeruginosa (261 aa), FASTA scores: opt: 475, E(): 3.8e-23,(36.85% identity in 247 aa overlap); etc. Also si [...] | 0.454 |
cyp125 | fadA5 | Rv3545c | Rv3546 | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Probable acetyl-CoA acetyltransferase FadA5 (acetoacetyl-CoA thiolase); Involved in the beta-oxidation of the cholesterol side chain. It is important for utilization of cholesterol as a sole carbon source in vitro and for full virulence in the chronic stage of mouse lung infection. Catalyzes the thiolysis of 3,22-dioxochol-4-en-24-oyl-CoA to yield 3-oxo-4-pregnene-20-carboxyl- CoA (3-OPC-CoA) and acetyl-CoA. Also able to use acetoacetyl-CoA (AcAcCoA) as substrate. | 0.932 |
cyp125 | fadD19 | Rv3545c | Rv3515c | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase); Catalyzes the activation of long-chain fatty acids as acyl- coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension. Also involved in the degradation of cholesterol via the degradation of the side chains of C-24 branched-chain sterols. Catalyzes the ATP-dependent CoA thioesterification of the sterol 3- oxocholest-4-en-26-oate to yield 3-oxocholest-4-en-26-oyl-CoA. It can also use 3beta-hydroxy-5-cholesten-26-oate. | 0.908 |
cyp125 | fadE26 | Rv3545c | Rv3504 | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Probable acyl-CoA dehydrogenase FadE26; Involved in the first cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of acyl-CoA ester side chains of (25S)-3-oxo-cholest-4-en-26-oyl-CoA (3-OCS-CoA) to yield (24E)-3-oxo-cholest-4,24-dien-26-oyl-CoA. Also able to dehydrogenate steroyl-CoA such as 3-oxo- chol-4-en-24-oyl-CoA (3-OCO-CoA) as well as 3-oxo-4-pregnene-20- carboxyl-CoA (3-OPC-CoA). It dehydrogenates only (25S)-OCS-CoA diastereomer ( [...] | 0.696 |
cyp125 | fadE27 | Rv3545c | Rv3505 | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Probable acyl-CoA dehydrogenase FadE27; Involved in the first cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of acyl-CoA ester side chains of (25S)-3-oxo-cholest-4-en-26-oyl-CoA (3-OCS-CoA) to yield (24E)-3-oxo-cholest-4,24-dien-26-oyl-CoA. Also able to dehydrogenate steroyl-CoA such as 3-oxo- chol-4-en-24-oyl-CoA (3-OCO-CoA) as well as 3-oxo-4-pregnene-20- carboxyl-CoA (3-OPC-CoA). It dehydrogenates only (25S)-OCS-CoA diastereomer. | 0.558 |
cyp125 | fadE34 | Rv3545c | Rv3573c | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Probable acyl-CoA dehydrogenase FadE34; Involved in the second cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the highly specific dehydrogenation of acyl-CoA ester side chains of 3- oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA) to yield 3-oxochol-4,22-dien-24- oyl-CoA. | 0.604 |
cyp125 | ltp3 | Rv3545c | Rv3523 | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Rv3523, (MTCY03C7.33c), len: 394 aa. Probable ltp3,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30037|AF0202 3-ketoacyl-CoA thiolase (ACAB-6) from Archaeoglobus fulgidus (380 aa) FASTA scores: opt: 782, E(): 1.7e-40, (38.35% identity in 386 aa overlap); Q9Y9A1|APE2384 long hypothetical non specific lipid-transfer protein (acethyl CoA synthetase) from Aeropyrum pernix (394 aa), FASTA scores: opt: 626, E(): 5.9e-31, (35.75% identity in 386 aa overlap); BAB59210|TVG0067506 lipid transfer protein from Thermoplasma volcanium (390 aa), FASTA scores: opt: 591,E(): [...] | 0.631 |
cyp125 | ltp4 | Rv3545c | Rv3522 | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | Rv3522, (MTV023.29), len: 354 aa. Possible ltp4,lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30103|AF0134 3-ketoacyl-CoA thiolase (ACAB-4) from Archaeoglobus fulgidus (398 aa) FASTA scores: opt: 352, E(): 5.3e-15, (30.45% identity in 381 aa overlap); O29295|AF0967 3-ketoacyl-CoA thiolase (ACAB-9) from Archaeoglobus fulgidus (400 aa) FASTA scores: opt: 312,E(): 1.8e-12, (28.05% identity in 367 aa overlap); O29294|AF0968 3-ketoacyl-CoA thiolase (ACAB-10) from Archaeoglobus fulgidus (388 aa), FASTA scores: opt: 293,E(): 2.9e-11, (25.9% identity in 309 aa overl [...] | 0.740 |
cyp142 | Rv3521 | Rv3518c | Rv3521 | Probable cytochrome P450 monooxygenase 142 Cyp142; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4- en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate. In vitro, Cyp142 catalyzes with equal preference the oxidation of both (25R)- and (25S)-26- hydroxycholest-4-en-3-one diastereomers to the corresponding carboxy [...] | Rv3521, (MTV023.28), len: 303 aa. Conserved hypothetical protein, similar to (although longer than) other conserved hypothetical proteins e.g. O29296|AF0966 from Archaeoglobus fulgidus (176 aa), FASTA scores: opt: 286, E(): 5.4e-11, (31.15% identity in 170 aa overlap); O30036|AF0203 from Archaeoglobus fulgidus (149 aa) FASTA scores: opt: 259, E(): 2.3e-09, (33.8% identity in 142 aa overlap); O29297|AF0965 from Archaeoglobus fulgidus (154 aa), FASTA scores: opt: 241, E(): 3.2e-08, (31.4% identity in 137 aa overlap); Q9Y995|APE2390 from Aeropyrum pernix (157 aa), FASTA scores: opt: 204, [...] | 0.614 |
cyp142 | cyp125 | Rv3518c | Rv3545c | Probable cytochrome P450 monooxygenase 142 Cyp142; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4- en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate. In vitro, Cyp142 catalyzes with equal preference the oxidation of both (25R)- and (25S)-26- hydroxycholest-4-en-3-one diastereomers to the corresponding carboxy [...] | Probable cytochrome P450 125 Cyp125; Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26- hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4- en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en- 3-one. Belongs to the cytochrome P450 family. | 0.943 |