UDP-galactopyranose mutase; Catalyzes the interconversion through a 2-keto intermediate of uridine diphosphogalactopyranose (UDP-GalP) into uridine diphosphogalactofuranose (UDP-GalF) which is a key building block for cell wall construction in Mycobacterium tuberculosis.

Decaprenyl-phosphate phosphoribosyltransferase; Involved in the biosynthesis of decaprenylphosphoryl arabinose (DPA) a precursor for arabinan synthesis in mycobacterial cell wall biosynthesis. Catalyzes the transfer of a 5-phosphoribosyl residue from phosphoribose diphosphate (pRpp) to decaprenyl phosphate (DP) to form decaprenylphosphoryl-5-phosphoribose (DPPR). The enzyme favors polyprenyl phosphate with 50-60 carbon atoms uses C-75 polyprenyl phosphate less efficiently than C-50 or C-60. Belongs to the UbiA prenyltransferase family.

Rv3783, (MTCY13D12.17), len: 280 aa. Probable rfbD,polysaccharide-transport integral membrane protein ABC transporter (see Braibant et al., 2000), involved in O-antigen/lipopolysaccharides (LPS) transport, equivalent to Q9CDA2|ML0112 putative ABC transporter component from Mycobacterium leprae (276 aa), FASTA scores: opt: 1646,E(): 4e-102, (84.3% identity in 280 aa overlap). Also highly similar to Q9PAF1|XF2567 ABC transporter permease protein from Xylella fastidiosa (267 aa), FASTA scores: opt: 723, E(): 7.6e-41, (41.3% identity in 259 aa overlap); and similar to others e.g. Q56902|RF [...]

Possible conserved transmembrane protein; Involved in the biosynthesis of decaprenylphosphoryl arabinose (DPA) a precursor for arabinan synthesis in mycobacterial cell wall biosynthesis. Could be involved in the dephosphorylation of decaprenylphosphoryl-5-phosphoribose (DPPR) to decaprenyl-phospho- ribose (DPR) (By similarity).

Rv3781, (MTCY13D12.15), len: 273 aa. Probable rfbE,polysaccharide-transport ATP-binding protein ABC transporter, involved in O-antigen/lipopolysaccharides (LPS) transport (see Braibant et al., 2000), equivalent to Q9CDA0|ML0114 putative ABC transporter ATP-binding component from Mycobacterium leprae (272 aa), FASTA scores: opt: 1581, E(): 3e-83, (91.4% identity in 267 aa overlap). Also highly similar to AAK71283 LPS/O-antigen export permease from Coxiella burnetii (258 aa), FASTA scores: opt: 793, E(): 2.5e-38, (45.45% identity in 253 aa overlap); Q9PAF0|XF2568 ABC transporter ATP-bind [...]

Arabinofuranosyltransferase AftA; Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the addition of the first key arabinofuranosyl (Araf) residue from the sugar donor decaprenyl-phospho-arabinose (DPA) on the C-5 of a 6-linked galactofuranosyl (Galf) of the galactan domain, thus 'priming' the galactan for further elaboration by other arabinofuranosyltransferases. It is not able to add an Araf residue to a terminal Galf. Belongs to the glycosyltransf [...]

Possible arabinofuranosyltransferase AftB; Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of arabinofuranosyl (Araf) residues from the sugar donor decaprenyl-phospho-arabinose (DPA) to the arabinan domain to form terminal beta-(1->2)-linked Araf residues, which marks the end point for AG arabinan biosynthesis before decoration with mycolic acids.

dTDP-4-dehydrorhamnose reductase; Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage. Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L- rhamnose (By similarity).

Decaprenyl-phosphate N-acetylglucosaminephosphotransferase; Involved in the biosynthesis of the disaccharide D-N- acetylglucosamine-L-rhamnose which plays an important role in the mycobacterial cell wall as a linker connecting arabinogalactan and peptidoglycan via a phosphodiester linkage. Catalyzes the transfer of the N-acetylglucosamine-1-phosphate (GlcNAc-1P) moiety from UDP-GlcNAc onto the carrier lipid decaprenyl phosphate (C50-P), yielding GlcNAc- pyrophosphoryl-decaprenyl (GlcNAc-PP-C50).