Hypothetical alanine and proline rich protein; Has a dual function in uptake of nutrients and induction of host cell death. The N-terminal domain (NTD) forms an outer membrane channel and is used for uptake of nutrients across the outer membrane. The secreted C-terminal toxic domain (TNT) acts as a glycohydrolase, which hydrolyzes the essential cellular coenzyme NAD(+) in the cytosol of infected macrophages, leading to necrotic host cell death. Both functions are required for survival, replication and cytotoxicity of M.tuberculosis in macrophages.

Rv3906c, (MTCY15F10.05), len: 169 aa. Conserved hypothetical protein, strongly related to Q50578|AT9S (sod related in Escherichia coli) from Mycobacterium tuberculosis strain aoyama B (155 aa), but apparently different as flanking sequences differ and shorter 43 aa,FASTA scores: opt: 548, E(): 1.3e-26, (79.4% identity in 102 aa overlap). Selfmarch results suggest that Rv3906c is not related to any other hypothetical protein from Mycobacterium tuberculosis strain H37Rv except itself. Shows also similarity with Q9VFR2|CG9297 hypothetical protein from Drosophila melanogaster (Fruit fly) ( [...]

Hypothetical protein; Antitoxin for tuberculosis necrotizing toxin (TNT). Acts by binding directly to TNT, which inhibits NAD(+) glycohydrolase activity of TNT and protects M.tuberculosis from self-poisoning.

Mycosin-4; Rv3449, (MTCY13E12.02), len: 455 aa. Probable mycP4,membrane-anchored serine protease (mycosin) (see citation below), similar to hypothetical unknowns or proteases from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK48366|MT3998 subtilase family protein from Mycobacterium tuberculosis strain CDC1551 (411 aa), FASTA scores: opt: 747, E(): 3.5e-33, (45.65% identity in 416 aa overlap); O05461|Rv3883c|MTCY15F10.29 membrane-anchored mycosin MYCP1 (446 aa), FASTA scores: opt: 747, E(): 3.8e-33, (45.45% identity in 451 aa overlap); O53695|Rv0291|MTV035.19 probable mem [...]

Rv3894c, (MTCY15F10.18), len: 1396 aa. EccC2, esx conserved component, ESX-2 type VII secretion system protein, possible membrane protein (possible transmembrane segments from aa ~37-85), similar to Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa),FASTA scores: opt: 652, E(): 2.2e-30, (27.85% identity in 1425 aa overlap); Q9CDD7|ML0052 hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 537, E(): 6.6e-24, (27.5% identity in 585 aa overlap) (similarity only with C-terminal end); Q9Z5I2|ML1543|MLCB596.28 possible SPOIIIE-family membrane prote [...]

Poly(A) polymerase PcnA; Rv3907c, (MTCY15F10.04), len: 480 aa. Probable pcnA,polynucleotide polymerase, equivalent to Q9CCY1|PCNA|ML2697 PCNA protein from Mycobacterium leprae (486 aa), FASTA scores: opt: 2713, E(): 4.3e-160, (84.1% identity in 478 aa overlap); and Q59534|PCNB POLYA polymerase from Mycobacterium leprae (411 aa) FASTA scores: opt: 2077, E(): 7.1e-121, (82.55% identity in 373 aa overlap). Also highly similar to many e.g. Q9X8T2|SCH24.18 putative RNA nucleotidyltransferase from Streptomyces coelicolor (483 aa), FASTA scores: opt: 1856, E(): 3.7e-107, (61.55% identity in 4 [...]

29 KDa antigen CFP29; Rv0798c, (MTCI429B.02), len: 265 aa. Cfp29, 29 kDa antigen (see citations below). Highly similar to Q45296|BLLINM18P_1|CAA63787.1|X93588 linocin M18 from Brevibacterium linens (266 aa), FASTA scores: (58.5% identity in 265 aa overlap). Also shows similarity with NP_228594.1|NC_000853 bacteriocin from Thermotoga maritima (262 aa).

Rv0724, (MTCY210.43), len: 623 aa. Possible sppA,protease IV (endopeptidase IV), equivalent (but longer 23 aa) to MLCB2492_24|O33003 endopeptidase IV from Mycobacterium leprae (602 aa). Also similar to others e.g. NP_419743.1|NC_002696 signal peptide peptidase SppA from Caulobacter crescentus (594 aa); P08395|SPPA_ECOLI|B1766 protease IV (endopeptidase) from Escherichia coli strain K-12 (618 aa), FASTA scores: opt: 582, E(): 8.9e-27, (34.1% identity in 525 aa overlap); etc. Belongs to peptidase family S49. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; [...]

Rv3020c, (MTV012.34c), len: 97 aa. EsxS, ESAT-6 like protein. PE-family related protein; distant member of the Mycobacterium tuberculosis PE family, similar to AAK44524|MT0300 PE family protein from M. tuberculosis strain CDC1551 (97 aa), FASTA scores: opt: 564, E(): 5.9e-30, (91.75% identity in 97 aa overlap). Has potential helix-turn-helix motif at positions 14-35. Seems to belong to the ESAT6 family (see Betts et al., 2002). Note that previously known as PE28. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al.,2004).