node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
Rv3566A | hsaB | Rv3566A | Rv3567c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | 0.826 |
Rv3566A | hsaC | Rv3566A | Rv3568c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | 3,4-DHSA dioxygenase; Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA). | 0.475 |
Rv3566A | hsaD | Rv3566A | Rv3569c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | 4,9-DHSA hydrolase; Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9- DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5- methyl-6-oxoocta-2,4-dienoic acid (HOPODA). Belongs to the AB hydrolase superfamily. HsaD family. | 0.414 |
Rv3566A | nat | Rv3566A | Rv3566c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.938 |
Rv3572 | hsaA | Rv3572 | Rv3570c | Unknown protein; Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | 0.565 |
Rv3572 | hsaB | Rv3572 | Rv3567c | Unknown protein; Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | 0.749 |
Rv3572 | hsaC | Rv3572 | Rv3568c | Unknown protein; Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). | 3,4-DHSA dioxygenase; Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA). | 0.675 |
Rv3572 | hsaD | Rv3572 | Rv3569c | Unknown protein; Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). | 4,9-DHSA hydrolase; Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9- DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5- methyl-6-oxoocta-2,4-dienoic acid (HOPODA). Belongs to the AB hydrolase superfamily. HsaD family. | 0.733 |
Rv3572 | kshB | Rv3572 | Rv3571 | Unknown protein; Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). | Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB; Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost- 1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4- androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4- BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4 [...] | 0.857 |
hsaA | Rv3572 | Rv3570c | Rv3572 | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Unknown protein; Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). | 0.565 |
hsaA | hsaB | Rv3570c | Rv3567c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | 0.999 |
hsaA | hsaC | Rv3570c | Rv3568c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | 3,4-DHSA dioxygenase; Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA). | 0.999 |
hsaA | hsaD | Rv3570c | Rv3569c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | 4,9-DHSA hydrolase; Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9- DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5- methyl-6-oxoocta-2,4-dienoic acid (HOPODA). Belongs to the AB hydrolase superfamily. HsaD family. | 0.999 |
hsaA | hsaE | Rv3570c | Rv3536c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Probable hydratase; Rv3536c, (MTCY03C7.20), len: 261 aa. Probable hsaE,hydratase, 2-oxo-hepta-3-ene-1,7-dioate hydratase or 2-keto-4-pentenoate hydratase. Indeed, highly similar to many 2-oxo-hepta-3-ene-1,7-dioate hydratases e.g. Q9CKS2|HPAH|PM1534 from Pasteurella multocida (267 aa) FASTA scores: opt: 743, E(): 1.5e-39, (45.5% identity in 266 aa overlap) Q9RZ31|DRA0122 from Deinococcus radiodurans (268 aa), FASTA scores: opt: 709, E(): 2e-37, (45.5% identity in 266 aa overlap); Q9HWQ4|HPCG|PA4127 from Pseudomonas aeruginosa (267 aa), FASTA scores: opt: 703,E(): 4.8e-37, (45.1% identi [...] | 0.754 |
hsaA | hsaF | Rv3570c | Rv3534c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Probable 4-hydroxy-2-oxovalerate aldolase (HOA); Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta- cleavage pathway for the degradation of aromatic compounds. Belongs to the 4-hydroxy-2-oxovalerate aldolase family. | 0.872 |
hsaA | kshB | Rv3570c | Rv3571 | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB; Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost- 1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4- androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4- BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4 [...] | 0.945 |
hsaA | kstD | Rv3570c | Rv3537 | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Probable dehydrogenase; Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids. Is also involved in the formation of 3-keto-1,4-diene-steroid from 3-keto- 4-ene-steroid. | 0.938 |
hsaA | nat | Rv3570c | Rv3566c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.697 |
hsaB | Rv3566A | Rv3567c | Rv3566A | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | Rv3566A, len: 88 aa. Hypothetical unknown protein. | 0.826 |
hsaB | Rv3572 | Rv3567c | Rv3572 | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | Unknown protein; Rv3572, (MTCY06G11.19), len: 176 aa. Unknown protein. Predicted to be an outer membrane protein (See Song et al., 2008). | 0.749 |