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| | fusA | Translation elongation factor G; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 s [...] (701 aa) |
| | rpsG | SSU ribosomal protein S7p (S5e); One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | rpsL | SSU ribosomal protein S12p (S23e); Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit. (124 aa) |
| | TASI_1558 | D-3-phosphoglycerate dehydrogenase; Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. (409 aa) |
| | rpoC | DNA-directed RNA polymerase beta' subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1440 aa) |
| | rpoB | DNA-directed RNA polymerase beta subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1370 aa) |
| | TASI_0002 | DNA polymerase III beta subunit; Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of [...] (394 aa) |
| | TASI_0015 | Coproporphyrinogen III oxidase, oxygen-independent; Belongs to the anaerobic coproporphyrinogen-III oxidase family. (455 aa) |
| | TASI_0016 | SAM-dependent methyltransferase. (282 aa) |
| | TASI_0017 | glutamyl-Q-tRNA synthetase. (270 aa) |
| | argS | Arginyl-tRNA synthetase. (560 aa) |
| | prfC | Peptide chain release factor 3; Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP. Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily. (534 aa) |
| | aspS | Aspartyl-tRNA synthetase; Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn); Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (598 aa) |
| | coaX | Pantothenate kinase type III, CoaX-like protein; Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis. (292 aa) |
| | glmU | N-acetylglucosamine-1-phosphate uridyltransferase; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. (460 aa) |
| | rpoH | RNA polymerase sigma factor RpoH; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. (292 aa) |
| | fmt | Methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. (320 aa) |
| | TASI_0084 | Putative lipoprotein. (158 aa) |
| | TASI_0135 | Hypothetical protein. (391 aa) |
| | atpB | ATP synthase A chain; Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane. (294 aa) |
| | atpE | ATP synthase C chain; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. (80 aa) |
| | TASI_0151 | ATP synthase B chain; Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0). Belongs to the ATPase B chain family. (135 aa) |
| | atpH | ATP synthase delta chain; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation; Belongs to the ATPase delta chain family. (180 aa) |
| | atpA | ATP synthase alpha chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit. Belongs to the ATPase alpha/beta chains family. (513 aa) |
| | atpG | ATP synthase gamma chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. (302 aa) |
| | atpD | ATP synthase beta chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits; Belongs to the ATPase alpha/beta chains family. (467 aa) |
| | atpC | ATP synthase epsilon chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. (142 aa) |
| | gatB | Aspartyl-tRNA(Asn) amidotransferase subunit B; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln); Belongs to the GatB/GatE family. GatB subfamily. (484 aa) |
| | gatA | Aspartyl-tRNA(Asn) amidotransferase subunit A; Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). (503 aa) |
| | rplJ | LSU ribosomal protein L10p (P0); Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. Belongs to the universal ribosomal protein uL10 family. (174 aa) |
| | gatC | Aspartyl-tRNA(Asn) amidotransferase subunit C; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln); Belongs to the GatC family. (102 aa) |
| | panC | Pantoate--beta-alanine ligase; Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate. Belongs to the pantothenate synthetase family. (283 aa) |
| | coaE | Dephospho-CoA kinase; Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; Belongs to the CoaE family. (211 aa) |
| | hemE | Uroporphyrinogen III decarboxylase; Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III. (354 aa) |
| | priA | Helicase PriA essential for oriC/DnaA-independent DNA replication; Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA; Belongs to the helicase family. PriA subfamily. (681 aa) |
| | rpmA | LSU ribosomal protein L27p; Belongs to the bacterial ribosomal protein bL27 family. (88 aa) |
| | rplU | LSU ribosomal protein L21p; This protein binds to 23S rRNA in the presence of protein L20; Belongs to the bacterial ribosomal protein bL21 family. (102 aa) |
| | apt | Adenine phosphoribosyltransferase; Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. (178 aa) |
| | TASI_0254 | Phosphomethylpyrimidine kinase. (269 aa) |
| | queC | Queuosine Biosynthesis QueC ATPase; Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Belongs to the QueC family. (222 aa) |
| | TASI_0259 | Queuosine biosynthesis QueD, PTPS-I. (143 aa) |
| | queE | Queuosine Biosynthesis QueE Radical SAM; Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7- deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds. (224 aa) |
| | hemA | Glutamyl-tRNA reductase; Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). (396 aa) |
| | prfA | Peptide chain release factor 1; Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. (355 aa) |
| | TASI_0280 | Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. (438 aa) |
| | TASI_0281 | 8-amino-7-oxononanoate synthase. (374 aa) |
| | bioD | Dethiobiotin synthetase; Catalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8- diaminopelargonic acid (DAPA) to form an ureido ring. (215 aa) |
| | TASI_0291 | NADPH dependent preQ0 reductase; Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). (286 aa) |
| | bioB | Biotin synthase; Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism; Belongs to the radical SAM superfamily. Biotin synthase family. (313 aa) |
| | TASI_0302 | DNA polymerase III epsilon subunit. (186 aa) |
| | TASI_0303 | DNA polymerase III epsilon subunit. (238 aa) |
| | purU | Formyltetrahydrofolate deformylase; Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4). (281 aa) |
| | metG | Methionyl-tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (702 aa) |
| | rplL | LSU ribosomal protein L7/L12 (P1/P2); Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family. (128 aa) |
| | rpmH | LSU ribosomal protein L34p; Belongs to the bacterial ribosomal protein bL34 family. (44 aa) |
| | TASI_1568 | Translation elongation factor Tu. (396 aa) |
| | nusG | Transcription antitermination protein NusG; Participates in transcription elongation, termination and antitermination. (180 aa) |
| | rplK | LSU ribosomal protein L11p (L12e); Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. (144 aa) |
| | rplA | LSU ribosomal protein L1p (L10Ae); Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (234 aa) |
| | dcd | Deoxycytidine triphosphate deaminase; Catalyzes the deamination of dCTP to dUTP. (187 aa) |
| | TASI_0321 | NAD(FAD)-utilizing dehydrogenase. (413 aa) |
| | thyA | Thymidylate synthase; Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. (264 aa) |
| | TASI_0327 | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. (168 aa) |
| | prs | Ribose-phosphate pyrophosphokinase; Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P); Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily. (317 aa) |
| | rplY | LSU ribosomal protein L25p; This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance. Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily. (212 aa) |
| | TASI_0339 | Peptidyl-tRNA hydrolase. (148 aa) |
| | coaD | Phosphopantetheine adenylyltransferase; Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate. Belongs to the bacterial CoaD family. (167 aa) |
| | ribB | 3,4-dihydroxy-2-butanone 4-phosphate synthase / GTP cyclohydrolase II; Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate; Belongs to the DHBP synthase family. (389 aa) |
| | ribH | 6,7-dimethyl-8-ribityllumazine synthase; Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. (229 aa) |
| | nusB | Transcription termination protein NusB; Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. (144 aa) |
| | thiL | Thiamine-monophosphate kinase; Catalyzes the ATP-dependent phosphorylation of thiamine- monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1; Belongs to the thiamine-monophosphate kinase family. (371 aa) |
| | TASI_0353 | Competence/damage-inducible protein CinA domain protein; Belongs to the CinA family. (163 aa) |
| | pyrF | Orotidine 5'-phosphate decarboxylase; Belongs to the OMP decarboxylase family. Type 2 subfamily. (283 aa) |
| | proS | Prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (582 aa) |
| | pyrC | Dihydroorotase; Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate. (358 aa) |
| | TASI_0432 | Putative phage protein; Possible primase 2 protein. (230 aa) |
| | TASI_0512 | BolA-like protein; Belongs to the BolA/IbaG family. (90 aa) |
| | pncB | Nicotinate phosphoribosyltransferase; Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP; Belongs to the NAPRTase family. (414 aa) |
| | accD | Acetyl-coenzyme A carboxyl transferase beta chain; Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA; Belongs to the AccD/PCCB family. (291 aa) |
| | rpmF | LSU ribosomal protein L32p; Belongs to the bacterial ribosomal protein bL32 family. (60 aa) |
| | lepA | Translation elongation factor LepA; Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner. (597 aa) |
| | TASI_0548 | Hypothetical protein. (151 aa) |
| | rpmG | LSU ribosomal protein L33p; Belongs to the bacterial ribosomal protein bL33 family. (55 aa) |
| | TASI_0567 | LSU ribosomal protein L28p. (66 aa) |
| | TASI_0570 | LSU ribosomal protein L31p; Belongs to the bacterial ribosomal protein bL31 family. (86 aa) |
| | nadD | Nicotinate-nucleotide adenylyltransferase; Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD). (195 aa) |
| | purD | Phosphoribosylamine--glycine ligase; Belongs to the GARS family. (428 aa) |
| | TASI_0586 | Adenylosuccinate lyase; Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily. (462 aa) |
| | TASI_0600 | dTDP-4-dehydrorhamnose reductase; Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose; Belongs to the dTDP-4-dehydrorhamnose reductase family. (311 aa) |
| | serC | Phosphoserine aminotransferase; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. (376 aa) |
| | cmk | Cytidylate kinase. (219 aa) |
| | TASI_0609 | SSU ribosomal protein S1p; Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence. (569 aa) |
| | TASI_0627 | Acetyl-coenzyme A synthetase. (469 aa) |
| | TASI_0630 | Putative membrane protein; Possible involvement in cytochrome functioning/assembly. (141 aa) |
| | TASI_0631 | DNA polymerase III delta subunit. (349 aa) |
| | leuS | Leucyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (884 aa) |
| | cysS | Cysteinyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (483 aa) |
| | accA | Acetyl-coenzyme A carboxyl transferase alpha chain; Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA. (320 aa) |
| | efp | Translation elongation factor P; Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. (184 aa) |
| | trpS | Tryptophanyl-tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (450 aa) |
| | rho | Transcription termination factor Rho; Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. (418 aa) |
| | alaS | Alanyl-tRNA synthetase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain. (874 aa) |
| | rpsP | SSU ribosomal protein S16p; Belongs to the bacterial ribosomal protein bS16 family. (138 aa) |
| | lysS | Lysyl-tRNA synthetase (class II); Belongs to the class-II aminoacyl-tRNA synthetase family. (508 aa) |
| | prfB | Peptide chain release factor 2; Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (327 aa) |
| | greB | Transcription elongation factor GreB; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length. (188 aa) |
| | rpmI | LSU ribosomal protein L35p; Belongs to the bacterial ribosomal protein bL35 family. (65 aa) |
| | rplT | LSU ribosomal protein L20p; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit. (119 aa) |
| | pheS | Phenylalanyl-tRNA synthetase alpha chain; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (340 aa) |
| | pheT | Phenylalanyl-tRNA synthetase beta chain; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (808 aa) |
| | purL | Phosphoribosylformylglycinamidine synthase; Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. (1333 aa) |
| | guaB | Inosine-5'-monophosphate dehydrogenase; Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Belongs to the IMPDH/GMPR family. (486 aa) |
| | guaA | GMP synthase (glutamine-hydrolyzing); Catalyzes the synthesis of GMP from XMP. (532 aa) |
| | TASI_0743 | Gamma-glutamyltranspeptidase. (564 aa) |
| | hemC | Porphobilinogen deaminase; Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. Belongs to the HMBS family. (313 aa) |
| | TASI_0749 | Hypothetical protein; Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III. (230 aa) |
| | TASI_0751 | HemY protein. (444 aa) |
| | valS | Valyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (956 aa) |
| | ndk | Nucleoside diphosphate kinase; Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate; Belongs to the NDK family. (135 aa) |
| | hisS | Histidyl-tRNA synthetase. (444 aa) |
| | purA | Adenylosuccinate synthetase; Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP; Belongs to the adenylosuccinate synthetase family. (429 aa) |
| | TASI_0769 | Xanthine-guanine phosphoribosyltransferase. (183 aa) |
| | rpsU | SSU ribosomal protein S21p; Belongs to the bacterial ribosomal protein bS21 family. (70 aa) |
| | dnaG | DNA primase; RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. (593 aa) |
| | rpoD | RNA polymerase sigma factor RpoD; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. (865 aa) |
| | rplS | LSU ribosomal protein L19p; This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. (126 aa) |
| | TASI_0802 | Ribonucleotide reductase of class Ia (aerobic), beta subunit. (377 aa) |
| | TASI_0834 | NAD synthetase; Glutamine amidotransferase chain of NAD synthetase. (338 aa) |
| | TASI_0835 | NAD synthetase; Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source. (212 aa) |
| | TASI_0840 | Hypothetical protein. (435 aa) |
| | pdxJ | Pyridoxine 5'-phosphate synthase; Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino- 2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate. (244 aa) |
| | TASI_0853 | Hypothetical protein. (261 aa) |
| | TASI_0862 | DNA polymerase III delta prime subunit. (279 aa) |
| | tmk | Thymidylate kinase; Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis; Belongs to the thymidylate kinase family. (204 aa) |
| | smpB | tmRNA-binding protein SmpB; Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switc [...] (149 aa) |
| | frr | Ribosome recycling factor; Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another; Belongs to the RRF family. (186 aa) |
| | pyrH | Uridylate kinase; Catalyzes the reversible phosphorylation of UMP to UDP. (237 aa) |
| | tsf | Translation elongation factor Ts; Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Belongs to the EF-Ts family. (292 aa) |
| | rpsB | SSU ribosomal protein S2p (SAe); Belongs to the universal ribosomal protein uS2 family. (263 aa) |
| | purF | Amidophosphoribosyltransferase; Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine; In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. (485 aa) |
| | TASI_0899 | Folylpolyglutamate synthase; Belongs to the folylpolyglutamate synthase family. (426 aa) |
| | folD | Methylenetetrahydrofolate dehydrogenase (NADP+); Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. (291 aa) |
| | kdsB | 3-deoxy-manno-octulosonate cytidylyltransferase; Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria. (253 aa) |
| | adk | Adenylate kinase; Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism; Belongs to the adenylate kinase family. (216 aa) |
| | rpsT | SSU ribosomal protein S20p; Binds directly to 16S ribosomal RNA. (87 aa) |
| | TASI_0937 | GNAT family acetyltransferase YjcF. (133 aa) |
| | TASI_0953 | GTP cyclohydrolase I type 1. (226 aa) |
| | TASI_0957 | DNA polymerase III alpha subunit. (1178 aa) |
| | gmk | Guanylate kinase; Essential for recycling GMP and indirectly, cGMP. (210 aa) |
| | rpoZ | DNA-directed RNA polymerase omega subunit; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (68 aa) |
| | TASI_0992 | Nicotinamidase. (173 aa) |
| | pyrG | CTP synthase; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates. (548 aa) |
| | infB | Translation initiation factor 2; One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (918 aa) |
| | nusA | Transcription termination protein NusA; Participates in both transcription termination and antitermination. (490 aa) |
| | TASI_1085 | Error-prone, lesion bypass DNA polymerase V (UmuC). (428 aa) |
| | purN | Phosphoribosylglycinamide formyltransferase; Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. (212 aa) |
| | TASI_1090 | Riboflavin kinase; FMN adenylyltransferase; Belongs to the ribF family. (321 aa) |
| | ileS | Isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (956 aa) |
| | TASI_1093 | Phosphopantothenoylcysteine decarboxylase; Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine; In the C-terminal section; belongs to the PPC synthetase family. (389 aa) |
| | dut | Deoxyuridine 5'-triphosphate nucleotidohydrolase; This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. (151 aa) |
| | TASI_1109 | Heme chaperone HemW; Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. Belongs to the anaerobic coproporphyrinogen-III oxidase family. (400 aa) |
| | TASI_1113 | Replicative DNA helicase; Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins. Belongs to the helicase family. DnaB subfamily. (476 aa) |
| | rplI | LSU ribosomal protein L9p; Binds to the 23S rRNA. (150 aa) |
| | rpsR | SSU ribosomal protein S18p; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (91 aa) |
| | TASI_1116 | Hypothetical protein; Binds single-stranded DNA at the primosome assembly site (PAS). (96 aa) |
| | rpsF | SSU ribosomal protein S6p; Binds together with S18 to 16S ribosomal RNA. (161 aa) |
| | folE2 | GTP cyclohydrolase I type 2; Converts GTP to 7,8-dihydroneopterin triphosphate. (269 aa) |
| | dxs | 1-deoxy-D-xylulose 5-phosphate synthase; Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D- xylulose-5-phosphate (DXP); Belongs to the transketolase family. DXPS subfamily. (619 aa) |
| | polA | DNA polymerase I; In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity; Belongs to the DNA polymerase type-A family. (888 aa) |
| | infC | Translation initiation factor 3; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (157 aa) |
| | thrS | Threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). (646 aa) |
| | queH | Hypothetical protein; Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr). (225 aa) |
| | TASI_1159 | Hypothetical protein. (499 aa) |
| | TASI_1162 | Hypothetical protein. (486 aa) |
| | rpsO | SSU ribosomal protein S15p (S13e); Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome. (89 aa) |
| | serS | Seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (445 aa) |
| | hemH | Ferrochelatase, protoheme ferro-lyase; Catalyzes the ferrous insertion into protoporphyrin IX. Belongs to the ferrochelatase family. (337 aa) |
| | nadK | NAD kinase; Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. (300 aa) |
| | TASI_1215 | Dihydropteroate synthase; Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8- dihydropteroate (H2Pte), the immediate precursor of folate derivatives. (283 aa) |
| | carB | Carbamoyl-phosphate synthase large chain; Belongs to the CarB family. (1081 aa) |
| | carA | Carbamoyl-phosphate synthase small chain; Belongs to the CarA family. (383 aa) |
| | TASI_1226 | Hypothetical protein. (129 aa) |
| | dnaX | DNA polymerase III subunits gamma and tau; DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. (387 aa) |
| | purM | Phosphoribosylformylglycinamidine cyclo-ligase. (351 aa) |
| | TASI_1242 | 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase. (163 aa) |
| | pyrD | Dihydroorotate dehydrogenase; Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor; Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. (330 aa) |
| | purH | IMP cyclohydrolase/Phosphoribosylaminoimidazolecarboxamide formyltransferase. (530 aa) |
| | TASI_1265 | Hypothetical protein. (72 aa) |
| | thiG | Thiazole biosynthesis protein ThiG; Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S. (263 aa) |
| | murD | UDP-N-acetylmuramoylalanine--D-glutamate ligase; Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA). Belongs to the MurCDEF family. (481 aa) |
| | glyQ | Glycyl-tRNA synthetase alpha chain. (302 aa) |
| | glyS | Glycyl-tRNA synthetase beta chain. (709 aa) |
| | glnS | Glutaminyl-tRNA synthetase. (584 aa) |
| | TASI_1312 | Putative ABC transport ATP-binding subunit. (557 aa) |
| | TASI_1315 | 4-hydroxythreonine-4-phosphate dehydrogenase; Belongs to the PdxA family. (339 aa) |
| | pdxH | Pyridoxamine 5'-phosphate oxidase; Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). (210 aa) |
| | purK | Phosphoribosylaminoimidazole carboxylase ATPase subunit; Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR). (385 aa) |
| | purE | Phosphoribosylaminoimidazole carboxylase catalytic subunit; Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR). (163 aa) |
| | purC | Phosphoribosylaminoimidazole-succinocarboxamide synthase; Belongs to the SAICAR synthetase family. (296 aa) |
| | upp | Uracil phosphoribosyltransferase; Catalyzes the conversion of uracil and 5-phospho-alpha-D- ribose 1-diphosphate (PRPP) to UMP and diphosphate. (212 aa) |
| | queA | S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). (350 aa) |
| | tgt | tRNA-guanine transglycosylase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, - Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the [...] (381 aa) |
| | TASI_1345 | Magnesium and cobalt efflux protein CorC. (300 aa) |
| | TASI_1363 | Riboflavin synthase alpha chain. (212 aa) |
| | TASI_1364 | Diaminohydroxyphosphoribosylaminopyrimidine deaminase; Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'- phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'- phosphate; In the C-terminal section; belongs to the HTP reductase family. (390 aa) |
| | tyrS | Tyrosyl-tRNA synthetase cluster 1; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. (407 aa) |
| | rpsI | SSU ribosomal protein S9p (S16e); Belongs to the universal ribosomal protein uS9 family. (130 aa) |
| | rplM | LSU ribosomal protein L13p (L13Ae); This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (142 aa) |
| | gltX | Glutamyl-tRNA synthetase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu); Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. (470 aa) |
| | TASI_1384 | Biotin carboxylase of acetyl-CoA carboxylase; This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA. (448 aa) |
| | TASI_1391 | Dihydroorotase. (428 aa) |
| | pyrB | Aspartate carbamoyltransferase; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family. (316 aa) |
| | TASI_1394 | UPF0301 protein YqgE; Belongs to the UPF0301 (AlgH) family. (178 aa) |
| | thiE | Thiamin-phosphate pyrophosphorylase; Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Belongs to the thiamine-phosphate synthase family. (213 aa) |
| | hemL | Glutamate-1-semialdehyde aminotransferase. (431 aa) |
| | TASI_1401 | 5-formyltetrahydrofolate cyclo-ligase; Belongs to the 5-formyltetrahydrofolate cyclo-ligase family. (189 aa) |
| | pyrE | Orotate phosphoribosyltransferase; Catalyzes the transfer of a ribosyl phosphate group from 5- phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP). (219 aa) |
| | murA | UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine; Belongs to the EPSP synthase family. MurA subfamily. (417 aa) |
| | def | Peptide deformylase; Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. (170 aa) |
| | lysA | Diaminopimelate decarboxylase; Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine. (425 aa) |
| | TASI_1480 | Porphobilinogen synthase; Belongs to the ALAD family. (336 aa) |
| | rplQ | LSU ribosomal protein L17p. (129 aa) |
| | rpoA | DNA-directed RNA polymerase alpha subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (327 aa) |
| | rpsD | SSU ribosomal protein S4p (S9e); One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. (207 aa) |
| | rpsK | SSU ribosomal protein S11p (S14e); Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (132 aa) |
| | rpsM | SSU ribosomal protein S13p (S18e); Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. Belongs to the universal ribosomal protein uS13 family. (121 aa) |
| | rpmJ | LSU ribosomal protein L36p; Belongs to the bacterial ribosomal protein bL36 family. (37 aa) |
| | infA | Translation initiation factor 1; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (72 aa) |
| | rplO | LSU ribosomal protein L15p (L27Ae); Binds to the 23S rRNA; Belongs to the universal ribosomal protein uL15 family. (147 aa) |
| | rpmD | LSU ribosomal protein L30p (L7e). (61 aa) |
| | rpsE | SSU ribosomal protein S5p (S2e); Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body. Belongs to the universal ribosomal protein uS5 family. (173 aa) |
| | rplR | LSU ribosomal protein L18p (L5e); This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. (121 aa) |
| | rplF | LSU ribosomal protein L6p (L9e); This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center; Belongs to the universal ribosomal protein uL6 family. (177 aa) |
| | rpsH | SSU ribosomal protein S8p (S15Ae); One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; Belongs to the universal ribosomal protein uS8 family. (131 aa) |
| | rpsN | SSU ribosomal protein S14p (S29e); Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site; Belongs to the universal ribosomal protein uS14 family. (101 aa) |
| | rplE | LSU ribosomal protein L5p (L11e); This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. (179 aa) |
| | rplX | LSU ribosomal protein L24p (L26e); One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit. (106 aa) |
| | rplN | LSU ribosomal protein L14p (L23e); Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome; Belongs to the universal ribosomal protein uL14 family. (122 aa) |
| | rpsQ | SSU ribosomal protein S17p (S11e); One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. (104 aa) |
| | rpmC | LSU ribosomal protein L29p (L35e); Belongs to the universal ribosomal protein uL29 family. (63 aa) |
| | rplP | LSU ribosomal protein L16p (L10e); Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs; Belongs to the universal ribosomal protein uL16 family. (137 aa) |
| | rpsC | SSU ribosomal protein S3p (S3e); Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation; Belongs to the universal ribosomal protein uS3 family. (281 aa) |
| | rplV | LSU ribosomal protein L22p (L17e); The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome. (112 aa) |
| | rpsS | SSU ribosomal protein S19p (S15e); Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (91 aa) |
| | rplB | LSU ribosomal protein L2p (L8e); One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Belongs to the universal ribosomal protein uL2 family. (275 aa) |
| | rplW | LSU ribosomal protein L23p (L23Ae); One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome; Belongs to the universal ribosomal protein uL23 family. (109 aa) |
| | rplD | LSU ribosomal protein L4p (L1e); Forms part of the polypeptide exit tunnel. (206 aa) |
| | rplC | LSU ribosomal protein L3p (L3e); One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (233 aa) |
| | rpsJ | SSU ribosomal protein S10p (S20e); Involved in the binding of tRNA to the ribosomes. Belongs to the universal ribosomal protein uS10 family. (103 aa) |
| | tuf | GTP-binding protein TypA/BipA; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa) |
