(25 nodes) tRNA wobble uridine modification, and Synthesis of diphthamide-EEF2 network (Trichoderma asperellum)

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Your Input:
	A0A2T3YQV3	Uncharacterized protein. (315 aa)
	A0A2T3YTL3	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. (572 aa)
	A0A2T3YVY3	Uncharacterized protein. (367 aa)
	NCS2	Cytoplasmic tRNA 2-thiolation protein 2; Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. (385 aa)
	A0A2T3YYM7	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2; Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2; Belongs to the DPH1/DPH2 family. DPH2 subfamily. (533 aa)
	A0A2T3Z0G3	Uncharacterized protein. (360 aa)
	A0A2T3Z2Q0	DPBB_1 domain-containing protein. (154 aa)
	uba4	Adenylyltransferase and sulfurtransferase uba4; Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and MOCS2A. Its N-terminus first activates urm1 and MOCS2A as acyl- adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and MOCS2A to form thiocarboxylation (- COSH) of their C-terminus. The reaction probably involves hydrogen sulfide [...] (462 aa)
	A0A2T3Z4W9	Uncharacterized protein. (368 aa)
	A0A2T3Z521	Uncharacterized protein. (933 aa)
	A0A2T3Z7W9	Uncharacterized protein. (289 aa)
	A0A2T3Z903	Elongator complex protein 1; Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. Belongs to the ELP1/IKA1 family. (1312 aa)
	A0A2T3Z9B2	Uncharacterized protein. (331 aa)
	URM1	Ubiquitin-related modifier 1; Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog UBA4. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target pr [...] (112 aa)
	A0A2T3ZBA0	Uncharacterized protein. (826 aa)
	A0A2T3ZBR4	Uncharacterized protein. (340 aa)
	A0A2T3ZDS6	Uncharacterized protein. (399 aa)
	NCS6	Cytoplasmic tRNA 2-thiolation protein 1; Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. (395 aa)
	A0A2T3ZIU6	Uncharacterized protein. (81 aa)
	A0A2T3ZIW5	Uncharacterized protein. (325 aa)
	A0A2T3ZKK3	Uncharacterized protein. (268 aa)
	A0A2T3ZKW8	Tr-type G domain-containing protein. (827 aa)
	A0A2T3ZLP0	TP_methylase domain-containing protein. (286 aa)
	A0A2T3ZQ40	Diphthami_syn_2 domain-containing protein. (794 aa)
	A0A2T3ZQA6	DPH-type MB domain-containing protein. (85 aa)

Your Current Organism:

Trichoderma asperellum

NCBI taxonomy Id: 1042311
Other names: T. asperellum CBS 433.97, Trichoderma asperellum CBS 433.97

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Browse interactions in tabular form:

node1	node2	node1 accession	node2 accession	node1 annotation	node2 annotation	score
A0A2T3YQV3	A0A2T3YTL3	A0A2T3YQV3	A0A2T3YTL3	Uncharacterized protein.	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	0.842
A0A2T3YQV3	A0A2T3Z2Q0	A0A2T3YQV3	A0A2T3Z2Q0	Uncharacterized protein.	DPBB_1 domain-containing protein.	0.558
A0A2T3YQV3	A0A2T3Z4W9	A0A2T3YQV3	A0A2T3Z4W9	Uncharacterized protein.	Uncharacterized protein.	0.593
A0A2T3YQV3	A0A2T3Z7W9	A0A2T3YQV3	A0A2T3Z7W9	Uncharacterized protein.	Uncharacterized protein.	0.575
A0A2T3YQV3	A0A2T3Z903	A0A2T3YQV3	A0A2T3Z903	Uncharacterized protein.	Elongator complex protein 1; Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. Belongs to the ELP1/IKA1 family.	0.711
A0A2T3YQV3	A0A2T3ZBA0	A0A2T3YQV3	A0A2T3ZBA0	Uncharacterized protein.	Uncharacterized protein.	0.714
A0A2T3YQV3	A0A2T3ZBR4	A0A2T3YQV3	A0A2T3ZBR4	Uncharacterized protein.	Uncharacterized protein.	0.575
A0A2T3YQV3	A0A2T3ZDS6	A0A2T3YQV3	A0A2T3ZDS6	Uncharacterized protein.	Uncharacterized protein.	0.575
A0A2T3YQV3	NCS6	A0A2T3YQV3	A0A2T3ZHI7	Uncharacterized protein.	Cytoplasmic tRNA 2-thiolation protein 1; Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.	0.471
A0A2T3YTL3	A0A2T3YQV3	A0A2T3YTL3	A0A2T3YQV3	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.842
A0A2T3YTL3	A0A2T3YVY3	A0A2T3YTL3	A0A2T3YVY3	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.726
A0A2T3YTL3	A0A2T3YYM7	A0A2T3YTL3	A0A2T3YYM7	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	2-(3-amino-3-carboxypropyl)histidine synthase subunit 2; Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2; Belongs to the DPH1/DPH2 family. DPH2 subfamily.	0.559
A0A2T3YTL3	A0A2T3Z0G3	A0A2T3YTL3	A0A2T3Z0G3	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.533
A0A2T3YTL3	A0A2T3Z2Q0	A0A2T3YTL3	A0A2T3Z2Q0	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	DPBB_1 domain-containing protein.	0.856
A0A2T3YTL3	A0A2T3Z4W9	A0A2T3YTL3	A0A2T3Z4W9	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.983
A0A2T3YTL3	A0A2T3Z521	A0A2T3YTL3	A0A2T3Z521	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.646
A0A2T3YTL3	A0A2T3Z7W9	A0A2T3YTL3	A0A2T3Z7W9	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.872
A0A2T3YTL3	A0A2T3Z903	A0A2T3YTL3	A0A2T3Z903	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Elongator complex protein 1; Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. Belongs to the ELP1/IKA1 family.	0.995
A0A2T3YTL3	A0A2T3Z9B2	A0A2T3YTL3	A0A2T3Z9B2	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.842
A0A2T3YTL3	A0A2T3ZBA0	A0A2T3YTL3	A0A2T3ZBA0	Elongator complex protein 3; Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation.	Uncharacterized protein.	0.983