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ilvA | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. (416 aa) | ||||
AKG03821.1 | Acetolactate synthase; Belongs to the TPP enzyme family. (542 aa) | ||||
thrB | Homoserine kinase; Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate; Belongs to the GHMP kinase family. Homoserine kinase subfamily. (307 aa) | ||||
AKG03875.1 | Threonine synthase; Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. (352 aa) | ||||
AKG03876.1 | Homoserine dehydrogenase. (432 aa) | ||||
ilvD | Dihydroxy-acid dehydratase; Belongs to the IlvD/Edd family. (564 aa) | ||||
AKG04612.1 | L-serine dehydratase, iron-sulfur-dependent subunit alpha. (289 aa) | ||||
AKG04613.1 | L-serine dehydratase, iron-sulfur-dependent subunit beta. (220 aa) | ||||
eutB | Hydroxyectoine utilization dehydratase EutB; Catalyzes the formation of 2-oxobutanoate from L-threonine; involved in ectoine utilization. (311 aa) | ||||
leuD | 3-isopropylmalate dehydratase small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. (194 aa) | ||||
leuC | 3-isopropylmalate dehydratase large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (472 aa) | ||||
leuB | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. (368 aa) | ||||
leuA | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. (513 aa) | ||||
ilvC | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. (342 aa) | ||||
AKG04751.1 | Acetolactate synthase small subunit. (173 aa) | ||||
AKG04752.1 | Acetolactate synthase, large subunit, biosynthetic type. (576 aa) | ||||
ilvE | Branched-chain-amino-acid transaminase; Acts on leucine, isoleucine and valine. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. (301 aa) | ||||
AKG05742.1 | Branched chain amino acid aminotransferase. (343 aa) | ||||
AKG04926.1 | Pyruvate oxidase; Catalyzes the formation of acetyl phosphate from pyruvate; Belongs to the TPP enzyme family. (571 aa) | ||||
AKG05059.1 | Glyoxal reductase. (274 aa) | ||||
AKG05161.1 | Aldehyde dehydrogenase; Belongs to the aldehyde dehydrogenase family. (483 aa) | ||||
AKG05255.1 | 2-hydroxy-acid oxidase. (465 aa) |