(18 nodes) (2017) Improving acarbose production and eliminating the by-product component C with an efficient genetic manipulation system of Actinoplanes sp. SE50110. network (Actinoplanes sp. SE50110)

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	int	Integrase; Belongs to the 'phage' integrase family. (406 aa)
	acbZ	Alpha-amylase; Glycosidases; Belongs to the glycosyl hydrolase 13 family. (1075 aa)
	acbW	ABC transporter ATP-binding protein. (357 aa)
	acbV	dTDP-4-keto-6-deoxy-glucose 4-aminotransferase; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. (431 aa)
	acbR	1-epi-valienol-1, 7-bisphosphate-1-adenylyltransferase. (363 aa)
	acbK	Acarbose 7-kinase; Catalyzes the phosphorylation of the alpha-glucosidase inhibitor acarbose. Phosphorylation of acarbose could be a resistance- like self-protection mechanism; Belongs to the carbohydrate kinase PfkB family. (299 aa)
	acbM	2-epi-5-epi-valiolone 7-kinase; Catalyzes the conversion of 2-epi-5-epi-valiolone to 2-epi-5- epi-valiolone 7-phosphate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the ROK (NagC/XylR) family. (359 aa)
	acbO	2-epi-5-epi-valiolone-7-phosphate 2-epimerase; Involved in the biosynthesis of the alpha-glucosidase inhibitor acarbose. Catalyzes the 2-epimerisation of 2-epi-5- epivaliolone 7-phosphate to yield 5-epi-valiolone 7-phosphate. Belongs to the hyi family. (270 aa)
	acbC	2-epi-5-epi-valiolone synthase; Catalyzes the cyclization of D-sedoheptulose 7-phosphate to 2-epi-5-epi-valiolone. Does not use ido-heptulose 7-phosphate and 3- deoxy-arabino-heptulosonate 7-phosphate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the sugar phosphate cyclases superfamily. EEVS family. (398 aa)
	acbB	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily. (320 aa)
	acbA	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family. (278 aa)
	acbD	Acarviose transferase (ATase); Belongs to the glycosyl hydrolase 13 family. (724 aa)
	int-2	Integrase; Belongs to the 'phage' integrase family. (388 aa)
	treY	(1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase; Maltooligosyl trehalose synthase. (755 aa)
	aglA	Alpha amylase catalytic region; Glycosidases. (519 aa)
	aglC	Alpha amylase catalytic region; Glycosidases. (534 aa)
	hrdB	RNA polymerase principal sigma factor hrdB; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. (333 aa)
	ACPL_2547	Alpha amylase catalytic region; Glycosidases. (557 aa)

Your Current Organism:

Actinoplanes sp. SE50110

NCBI taxonomy Id: 134676
Other names: A. sp. SE50/110, Actinoplanes sp. (strain 50/110), Actinoplanes sp. 50/110, Actinoplanes sp. SE50/110

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Browse interactions in tabular form:

node1	node2	node1 accession	node2 accession	node1 annotation	node2 annotation	score
ACPL_2547	acbC	ACPL_2547	ACPL_3680	Alpha amylase catalytic region; Glycosidases.	2-epi-5-epi-valiolone synthase; Catalyzes the cyclization of D-sedoheptulose 7-phosphate to 2-epi-5-epi-valiolone. Does not use ido-heptulose 7-phosphate and 3- deoxy-arabino-heptulosonate 7-phosphate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the sugar phosphate cyclases superfamily. EEVS family.	0.400
ACPL_2547	acbZ	ACPL_2547	ACPL_3663	Alpha amylase catalytic region; Glycosidases.	Alpha-amylase; Glycosidases; Belongs to the glycosyl hydrolase 13 family.	0.465
ACPL_2547	aglA	ACPL_2547	ACPL_6838	Alpha amylase catalytic region; Glycosidases.	Alpha amylase catalytic region; Glycosidases.	0.905
ACPL_2547	aglC	ACPL_2547	ACPL_1259	Alpha amylase catalytic region; Glycosidases.	Alpha amylase catalytic region; Glycosidases.	0.903
ACPL_2547	treY	ACPL_2547	ACPL_6622	Alpha amylase catalytic region; Glycosidases.	(1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase; Maltooligosyl trehalose synthase.	0.409
acbA	acbB	ACPL_3682	ACPL_3681	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	0.998
acbA	acbC	ACPL_3682	ACPL_3680	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	2-epi-5-epi-valiolone synthase; Catalyzes the cyclization of D-sedoheptulose 7-phosphate to 2-epi-5-epi-valiolone. Does not use ido-heptulose 7-phosphate and 3- deoxy-arabino-heptulosonate 7-phosphate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the sugar phosphate cyclases superfamily. EEVS family.	0.546
acbA	acbD	ACPL_3682	ACPL_3684	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	Acarviose transferase (ATase); Belongs to the glycosyl hydrolase 13 family.	0.503
acbA	acbM	ACPL_3682	ACPL_3676	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	2-epi-5-epi-valiolone 7-kinase; Catalyzes the conversion of 2-epi-5-epi-valiolone to 2-epi-5- epi-valiolone 7-phosphate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the ROK (NagC/XylR) family.	0.404
acbA	acbO	ACPL_3682	ACPL_3679	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	2-epi-5-epi-valiolone-7-phosphate 2-epimerase; Involved in the biosynthesis of the alpha-glucosidase inhibitor acarbose. Catalyzes the 2-epimerisation of 2-epi-5- epivaliolone 7-phosphate to yield 5-epi-valiolone 7-phosphate. Belongs to the hyi family.	0.400
acbA	acbV	ACPL_3682	ACPL_3667	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	dTDP-4-keto-6-deoxy-glucose 4-aminotransferase; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.	0.589
acbA	acbW	ACPL_3682	ACPL_3666	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	ABC transporter ATP-binding protein.	0.443
acbA	acbZ	ACPL_3682	ACPL_3663	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	Alpha-amylase; Glycosidases; Belongs to the glycosyl hydrolase 13 family.	0.428
acbB	acbA	ACPL_3681	ACPL_3682	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	Glucose-1-phosphate thymidylyltransferase; Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis; Belongs to the glucose-1-phosphate thymidylyltransferase family.	0.998
acbB	acbC	ACPL_3681	ACPL_3680	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	2-epi-5-epi-valiolone synthase; Catalyzes the cyclization of D-sedoheptulose 7-phosphate to 2-epi-5-epi-valiolone. Does not use ido-heptulose 7-phosphate and 3- deoxy-arabino-heptulosonate 7-phosphate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the sugar phosphate cyclases superfamily. EEVS family.	0.487
acbB	acbD	ACPL_3681	ACPL_3684	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	Acarviose transferase (ATase); Belongs to the glycosyl hydrolase 13 family.	0.580
acbB	acbM	ACPL_3681	ACPL_3676	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	2-epi-5-epi-valiolone 7-kinase; Catalyzes the conversion of 2-epi-5-epi-valiolone to 2-epi-5- epi-valiolone 7-phosphate. Involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the ROK (NagC/XylR) family.	0.405
acbB	acbR	ACPL_3681	ACPL_3670	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	1-epi-valienol-1, 7-bisphosphate-1-adenylyltransferase.	0.412
acbB	acbV	ACPL_3681	ACPL_3667	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	dTDP-4-keto-6-deoxy-glucose 4-aminotransferase; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.	0.957
acbB	acbW	ACPL_3681	ACPL_3666	dTDP-glucose 4,6-dehydratase; Probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D- xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.	ABC transporter ATP-binding protein.	0.430