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| | AQM61163.1 | Nadp_idh_euk: isocitrate dehydrogenase, NADP-dependent; [C] COG0538 Isocitrate dehydrogenases; Belongs to the isocitrate and isopropylmalate dehydrogenases family. (402 aa) |
| | AQM59683.1 | Pyruvate kinase, barrel domain protein; [G] COG0469 Pyruvate kinase; Belongs to the pyruvate kinase family. (345 aa) |
| | dapD | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase; Catalyzes the transfer of an acetyl group from acetyl-CoA to tetrahydrodipicolinate. (236 aa) |
| | AQM60933.1 | hisJ_fam: histidinol phosphate phosphatase, HisJ family protein; [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family; Belongs to the PHP hydrolase family. HisK subfamily. (257 aa) |
| | aspD | Asp_4_decarbox: aspartate 4-decarboxylase; [E] COG0436 Aspartate/tyrosine/aromatic aminotransferase. (542 aa) |
| | hisE | histidine_hisI: phosphoribosyl-ATP diphosphatase; [E] COG0140 Phosphoribosyl-ATP pyrophosphohydrolase. (109 aa) |
| | AQM61372.1 | phosphoribosyl-AMP cyclohydrolase family protein; Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP; Belongs to the PRA-CH family. (79 aa) |
| | hisF | Imidazoleglycerol phosphate synthase, cyclase subunit; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit. (253 aa) |
| | hisA | [E] COG0106 Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase. (245 aa) |
| | hisH | Imidazole glycerol phosphate synthase, glutamine amidotransferase subunit; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF. (203 aa) |
| | hisD | Histidinol dehydrogenase; Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine. (431 aa) |
| | hisG | ATP phosphoribosyltransferase; Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity. Belongs to the ATP phosphoribosyltransferase family. Short subfamily. (210 aa) |
| | hisZ | ATP phosphoribosyltransferase, regulatory subunit; Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine. (377 aa) |
| | AQM61509.1 | [R] COG4492 ACT domain-containing protein; Belongs to the UPF0735 family. (145 aa) |
| | dapB | 4-hydroxy-tetrahydrodipicolinate reductase; Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate; Belongs to the DapB family. (251 aa) |
| | thrB | Homoserine kinase; Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate; Belongs to the GHMP kinase family. Homoserine kinase subfamily. (301 aa) |
| | dapA | 4-hydroxy-tetrahydrodipicolinate synthase; Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). (292 aa) |
| | asd | Aspartate-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. (329 aa) |
| | ilvA | Threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. (399 aa) |
| | pyk | Pyruv_kin: pyruvate kinase; [G] COG0469 Pyruvate kinase; Belongs to the pyruvate kinase family. (472 aa) |
| | metK | Methionine adenosyltransferase; Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. (391 aa) |
| | rpiB | rpiB: ribose 5-phosphate isomerase B; [G] COG0698 Ribose 5-phosphate isomerase RpiB. (149 aa) |
| | AQM58747.1 | Diaminopimelate dehydrogenase; Catalyzes the reversible NADPH-dependent reductive amination of L-2-amino-6-oxopimelate, the acyclic form of L- tetrahydrodipicolinate, to generate the meso compound, D,L-2,6- diaminopimelate. (326 aa) |
| | gltA | gltA: glutamate synthase (NADPH), homotetrameric; [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases. (464 aa) |
| | mtnN | MTA/SAH nucleosidase; Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'- methylthioribose and S-ribosylhomocysteine, respectively. Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. (263 aa) |
| | ilvC | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. (334 aa) |
| | ilvB | Acolac_lg: acetolactate synthase, large subunit, biosynthetic type; [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]. (556 aa) |
| | ilvD | ilvD: dihydroxy-acid dehydratase; [EG] COG0129 Dihydroxyacid dehydratase/phosphogluconate dehydratase; Belongs to the IlvD/Edd family. (552 aa) |
| | leuB | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. (361 aa) |
| | leuD | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 2 subfamily. (161 aa) |
| | leuC | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (419 aa) |
| | AQM60219.1 | HMGL-like family protein; [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases; Belongs to the alpha-IPM synthase/homocitrate synthase family. (530 aa) |
| | ilvN | Acolac_sm: acetolactate synthase, small subunit; [E] COG0440 Acetolactate synthase, small (regulatory) subunit. (166 aa) |
| | prs | Ribose-phosphate diphosphokinase family protein; Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P); Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily. (319 aa) |
| | AQM61128.1 | Aconitase C-terminal domain protein; [C] COG1048 Aconitase A. (637 aa) |
| | AQM61242.1 | [R] COG4492 ACT domain-containing protein; Belongs to the UPF0735 family. (146 aa) |
| | AQM61094.1 | NAD-dependent glycerol-3-phosphate dehydrogenase family protein; [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases. (411 aa) |
| | AQM60451.1 | Glutamine synthetase, catalytic domain protein; [E] COG0174 Glutamine synthetase. (633 aa) |
| | proA | Glutamate-5-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. Belongs to the gamma-glutamyl phosphate reductase family. (415 aa) |
| | proB | Glutamate 5-kinase; Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate. (272 aa) |
| | rpe | Rpe: ribulose-phosphate 3-epimerase; [G] COG0036 Pentose-5-phosphate-3-epimerase. (216 aa) |
| | proC | Pyrroline-5-carboxylate reductase; Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. (266 aa) |
| | AQM60497.1 | D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain protein; [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases; Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. (301 aa) |
| | AQM59539.1 | C_S_lyase_PatB: C-S lyase family protein; [E] COG1168 Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities. (396 aa) |
| | mtnN-2 | MTA/SAH nucleosidase; Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'- methylthioribose and S-ribosylhomocysteine, respectively. Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. (230 aa) |
| | AQM60595.1 | Hypothetical protein; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain protein; [G] COG3959 Transketolase, N-terminal subunit. (274 aa) |
| | AQM59421.1 | Transketolase, C-terminal domain protein; [G] COG3958 Transketolase, C-terminal subunit. (314 aa) |
| | hisC | hisC: histidinol-phosphate transaminase; [E] COG0079 Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily. (367 aa) |
| | pfkA | 6-phosphofructokinase; Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. (319 aa) |
| | pyk-2 | Pyruv_kin: pyruvate kinase; [G] COG0469 Pyruvate kinase; Belongs to the pyruvate kinase family. (475 aa) |
| | pyk-3 | Pyruv_kin: pyruvate kinase; [G] COG0469 Pyruvate kinase; Belongs to the pyruvate kinase family. (471 aa) |
| | lysA | Diaminopimelate decarboxylase; Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine. (431 aa) |
| | AQM59468.1 | Hypothetical protein; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain protein; [G] COG3959 Transketolase, N-terminal subunit. (272 aa) |
| | AQM60666.1 | Transketolase, C-terminal domain protein; [G] COG3958 Transketolase, C-terminal subunit. (307 aa) |
| | NPD11_249 | Asp_kinase: aspartate kinase domain protein; [E] COG0527 Aspartokinases; Belongs to the aspartokinase family. (399 aa) |
| | aroF | DAHP_synth_Bsub: 3-deoxy-7-phosphoheptulonate synthase; [E] COG2876 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase. (337 aa) |
| | aroB | 3-dehydroquinate synthase; Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ). (350 aa) |
| | aroC | Chorismate synthase; Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system. (360 aa) |
| | AQM61380.1 | CM_mono_grmpos: chorismate mutase; [E] COG0077 Prephenate dehydratase. (379 aa) |
| | aroK | ATPase associated with various cellular activities family protein; Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate; Belongs to the shikimate kinase family. (170 aa) |
| | aroQ | 3-dehydroquinate dehydratase, type II; Catalyzes a trans-dehydration via an enolate intermediate. Belongs to the type-II 3-dehydroquinase family. (141 aa) |
| | aroF-2 | DAHP_synth_Bsub: 3-deoxy-7-phosphoheptulonate synthase; [E] COG2876 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase. (337 aa) |
| | argD | argD: transaminase, acetylornithine/succinylornithine family protein; [E] COG4992 Ornithine/acetylornithine aminotransferase; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily. (385 aa) |
| | argB | Acetylglutamate kinase; Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate; Belongs to the acetylglutamate kinase family. ArgB subfamily. (286 aa) |
| | argJ | Glutamate N-acetyltransferase/amino-acid acetyltransferase; Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate. Belongs to the ArgJ family. (402 aa) |
| | argC | N-acetyl-gamma-glutamyl-phosphate reductase; Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. (345 aa) |
| | argH | argH: argininosuccinate lyase; [E] COG0165 Argininosuccinate lyase. (466 aa) |
| | argG | argG: argininosuccinate synthase; [E] COG0137 Argininosuccinate synthase; Belongs to the argininosuccinate synthase family. Type 1 subfamily. (406 aa) |
| | sdaAB | Sda_beta: L-serine dehydratase, iron-sulfur-dependent, beta subunit; [E] COG1760 L-serine deaminase. (227 aa) |
| | sdaAA | Sda_alpha: L-serine dehydratase, iron-sulfur-dependent, alpha subunit; [E] COG1760 L-serine deaminase. (292 aa) |
| | cysK | cysK: cysteine synthase A; [E] COG0031 Cysteine synthase; Belongs to the cysteine synthase/cystathionine beta- synthase family. (301 aa) |
| | cysE | cysE: serine O-acetyltransferase; [E] COG1045 Serine acetyltransferase. (193 aa) |
| | gap | GAPDH-I: glyceraldehyde-3-phosphate dehydrogenase, type I; [G] COG0057 Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase; Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. (333 aa) |
| | pgk | [G] COG0126 3-phosphoglycerate kinase; Belongs to the phosphoglycerate kinase family. (396 aa) |
| | tpiA | Triose-phosphate isomerase; Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P); Belongs to the triosephosphate isomerase family. (248 aa) |
| | gpmI | Phosphoglycerate mutase; Catalyzes the interconversion of 2-phosphoglycerate and 3- phosphoglycerate. (510 aa) |
| | eno | Phosphopyruvate hydratase; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis; Belongs to the enolase family. (431 aa) |
| | gltA-2 | gltA: glutamate synthase (NADPH), homotetrameric; [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases. (464 aa) |
| | ilvE | ilvE_II: branched-chain amino acid aminotransferase; [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase. (340 aa) |
| | prs-2 | ribP_PPkin: ribose-phosphate diphosphokinase family protein; [FE] COG0462 Phosphoribosylpyrophosphate synthetase; Belongs to the ribose-phosphate pyrophosphokinase family. (366 aa) |
| | fba | Fruc_bis_ald_: fructose-1,6-bisphosphate aldolase, class II; [G] COG0191 Fructose/tagatose bisphosphate aldolase. (287 aa) |
| | metA | Homoserine O-succinyltransferase; Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine; Belongs to the MetA family. (305 aa) |
| | pfp | Phosphofructokinase family protein; Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions. (405 aa) |
| | AQM60970.1 | Beta-eliminating lyase family protein; [E] COG0436 Aspartate/tyrosine/aromatic aminotransferase. (397 aa) |
| | leuA | leuA_bact: 2-isopropylmalate synthase; [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases; Belongs to the alpha-IPM synthase/homocitrate synthase family. (500 aa) |
| | NPD11_31 | ACT domain protein; [E] COG0460 Homoserine dehydrogenase. (432 aa) |
| | dapF | Diaminopimelate epimerase; Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan. (272 aa) |
| | trpB | Tryptophan synthase, beta subunit; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. (400 aa) |
| | NPD11_737 | hisJ_fam: histidinol phosphate phosphatase, HisJ family protein; [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family; Belongs to the PHP hydrolase family. HisK subfamily. (258 aa) |
| | glyA | Beta-eliminating lyase family protein; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (411 aa) |
| | asnA | asnA: aspartate--ammonia ligase; [E] COG2502 Asparagine synthetase A. (337 aa) |
| | pfkA1 | Phosphofructokinase family protein; Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis; Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily. (366 aa) |
