Your Input: | |
| | spr0253 | Conserved hypothetical protein. (92 aa) |
| | rlmH | Conserved hypothetical protein; Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA; Belongs to the RNA methyltransferase RlmH family. (159 aa) |
| | trpS | Tryptophanyl-tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (341 aa) |
| | recF | Recombination protein RecF; The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP (By similarity). Plays no role in chromosomal or plasmid transformation but is required for resolution of chromosome dimers occurring as intermediates in the formation of merodiploids by transformation. (365 aa) |
| | comFA | Involved in transformation; required for DNA uptake but not for binding; Related to ATP-dependent RNA/DNA helicases. (432 aa) |
| | dnaC | Replicative DNA helicase; Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. Belongs to the helicase family. DnaB subfamily. (450 aa) |
| | spr1994 | Conserved hypothetical protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines; Belongs to the dus family. (336 aa) |
| | spr1984 | Transposase, uncharacterized, truncation. (330 aa) |
| | hisS | Histidyl-tRNA synthetase. (429 aa) |
| | aspS | Aspartyl-tRNA synthetase (aspartate-tRNA ligase); Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (587 aa) |
| | tyrS | Tyrosyl-tRNA synthetase 1; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (418 aa) |
| | argS | Arginyl-tRNA synthetase(arginine--tRNA ligase) (ARGRS). (563 aa) |
| | hexA | DNA mismatch repair protein HexA; This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. (857 aa) |
| | spr1886 | Degenerate transposase. (104 aa) |
| | gltX | Glutamyl-tRNA synthetase (glutamate--tRNA ligase); Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu); Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. (486 aa) |
| | spr1876 | Hypothetical protein; Low similarity to phosphoglycolate phosphatase. (206 aa) |
| | tgt | tRNA-guanine transglycosylase (guanine insertion enzyme); Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' [...] (380 aa) |
| | spr1855 | Conserved hypothetical protein. (317 aa) |
| | rnpA | Ribonuclease P - protein component; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (123 aa) |
| | sga | Hexulose-6-phosphate isomerase. (287 aa) |
| | rluD-3 | Ribosomal large subunit pseudouridine synthase D. (291 aa) |
| | nusG | Transcription antitermination factor; Participates in transcription elongation, termination and antitermination. (183 aa) |
| | spr1805 | Conserved hypothetical protein. (257 aa) |
| | rnmV | Conserved hypothetical protein; Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step; Belongs to the ribonuclease M5 family. (186 aa) |
| | ksgA | Dimethyladenosine transferase; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (290 aa) |
| | cbf1 | Cmp-binding-factor 1. (334 aa) |
| | yjfA | Possible rRNA/tRNA methylase; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (252 aa) |
| | spr1784 | Conserved hypothetical protein; Probable methylase. (179 aa) |
| | endA | DNA-entry nuclease (competence-specific nuclease); By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed; Belongs to the DNA/RNA non-specific endonuclease family. (274 aa) |
| | rpoB | DNA-dependent RNA polymerase subunit beta; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1216 aa) |
| | rpoC | DNA-dependent RNA polymerase; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1225 aa) |
| | spr1761 | Conserved hypothetical protein. (147 aa) |
| | recA | DNA recombination/repair; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs (By similarity). It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity). Required for DNA transformation; protects transforming DNA from degradation, possibly in combination with DprA. Present at 15,000-30,000 monomers per competent cell. (388 aa) |
| | ssbB | Single-stranded DNA-binding protein; Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism. (131 aa) |
| | spr1717 | Conserved hypothetical protein; Similar to RNA methyltransferase; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. (451 aa) |
| | xerD-2 | Recombinase, site specific; Putative tyrosine recombinase. Not involved in the cutting and rejoining of the recombining DNA molecules on dif(SL) site. (246 aa) |
| | scpA | Conserved hypothetical protein; Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves. Belongs to the ScpA family. (242 aa) |
| | scpB | Conserved hypothetical protein; Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves. Belongs to the ScpB family. (189 aa) |
| | rluB | Ribosomal large subunit pseudouridine synthase B; Belongs to the pseudouridine synthase RsuA family. (240 aa) |
| | dpnC | Type II restriction enzyme DpnI (dpnC); Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2. (254 aa) |
| | dpnD | Restriction system of S. pneumoniae. (153 aa) |
| | exoA | Exodeoxyribonuclease; In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA; Belongs to the DNA repair enzymes AP/ExoA family. (275 aa) |
| | cshA | Chromosome segregation helicase. (423 aa) |
| | spr1613 | Hypothetical protein. (68 aa) |
| | spr1607 | Conserved hypothetical protein; Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit. (251 aa) |
| | tmcAL | Conserved hypothetical protein; Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of elongator tRNA(Met), using acetate and ATP as substrates. First activates an acetate ion to form acetyladenylate (Ac- AMP) and then transfers the acetyl group to tRNA to form ac(4)C34. (365 aa) |
| | spr1585 | Conserved hypothetical protein. (84 aa) |
| | rny | Conserved hypothetical protein; Endoribonuclease that initiates mRNA decay. Belongs to the RNase Y family. (537 aa) |
| | rpoZ | Hypothetical protein; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (104 aa) |
| | priA | Primosomal replication factor Y; Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA; Belongs to the helicase family. PriA subfamily. (798 aa) |
| | fmt | Methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. (311 aa) |
| | sunL | rRNA methylase; Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA. (438 aa) |
| | dnaI | Primosome component (helicase loader). (298 aa) |
| | recG | Branch migration of Holliday junctions, junction-specific DNA helicase; Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y- DNA); Belongs to the helicase family. RecG subfamily. (671 aa) |
| | recR | Recombination protein RecR; May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO (By similarity). Plays no role in chromosomal or plasmid transformation but is required for resolution of chromosome dimers occurring as intermediates in the formation of merodiploids by transformation ; Belongs to the RecR family. (198 aa) |
| | ileS | Isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (930 aa) |
| | dtd | Conserved hypothetical protein; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. Belongs to the DTD family. (147 aa) |
| | thrS | Threonyl-tRNA synthetase 1; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (660 aa) |
| | spr1463 | Conserved hypothetical protein. (225 aa) |
| | truA | tRNA pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (249 aa) |
| | spr1446 | Degenerate transposase (orf2). (162 aa) |
| | cshA-2 | Conserved hypothetical protein; DEAD-box RNA helicase possibly involved in RNA degradation. Unwinds dsRNA in both 5'- and 3'-directions, has RNA-dependent ATPase activity; Belongs to the DEAD box helicase family. CshA subfamily. (524 aa) |
| | cca | tRNA nucleotidyltransferase; Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. (399 aa) |
| | asnS | Asparaginyl-tRNA synthetase (asparagine--tRNA ligase). (447 aa) |
| | ssbA | Single stranded binding protein; Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism. (156 aa) |
| | greA | Transcription elongation factor GreA; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (163 aa) |
| | IS1239 | Transposase. (335 aa) |
| | spr1339 | Degenerate transposase (orf2). (162 aa) |
| | glyQ | Glycyl-tRNA synthetase alpha chain. (305 aa) |
| | glyS | Glycyl-tRNA synthetase beta chain. (678 aa) |
| | ogt | O6-methylguanine-DNA methyltransferase; Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. (176 aa) |
| | trmH | tRNA (guanosine-2'-O-)-methyltransferase, TrmH family; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (253 aa) |
| | spnIM | DNA modification methyltransferase; Belongs to the N(4)/N(6)-methyltransferase family. (392 aa) |
| | spr1286 | Type II restriction endonuclease, uncharacterized, truncation. (63 aa) |
| | spr1285 | Type II restriction endonuclease, uncharacterized, truncation. (60 aa) |
| | queA | S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). (346 aa) |
| | spr1259 | Conserved hypothetical protein. (267 aa) |
| | alaS | Alanyl-tRNA synthetase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain. (872 aa) |
| | gidB | Glucose inhibited division protein B; Specifically methylates the N7 position of a guanine in 16S rRNA; Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family. (245 aa) |
| | nth | Endonuclease III (DNA repair); DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'- phosphate. (209 aa) |
| | pyrR | Transcriptional attenuation of the pyrimidine operon / uracil phosphoribosyltransferase activity; Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes. Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily. (173 aa) |
| | topA | DNA topoisomerase I; Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supe [...] (701 aa) |
| | rncS | Ribonuclease III; Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. (232 aa) |
| | smc | Chromosome condensation and segregation SMC protein; Required for chromosome condensation and partitioning. Belongs to the SMC family. (1179 aa) |
| | uvrB | Exonuclease ABC - subunit B; The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate [...] (662 aa) |
| | mutY | Similar to A/G-specific adenine glycosylase; Adenine glycosylase active on G-A mispairs. (391 aa) |
| | spr1102 | Uncharacterized restriction enzyme, interrupted-N. (625 aa) |
| | spr1101 | Uncharacterized restriction enzyme, interrupted-C. (1084 aa) |
| | gyrA | DNA gyrase subunit A; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (822 aa) |
| | truB | tRNA pseudouridine 5S synthase; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (292 aa) |
| | xseA | Exodeoxyribonuclease large subunit; Bidirectionally degrades single-stranded DNA into large acid- insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides; Belongs to the XseA family. (448 aa) |
| | xseB | Exodeoxyribonuclease small subunit; Bidirectionally degrades single-stranded DNA into large acid- insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides; Belongs to the XseB family. (70 aa) |
| | recN | DNA repair and genetic recombination; May be involved in recombinational repair of damaged DNA. (555 aa) |
| | pphA | Serine/threonine protein phosphatase. (242 aa) |
| | spr1078 | Degenerate transposase (orf2). (132 aa) |
| | nrdF | Nonessential ribonucleoside-diP reductase 2, subunit beta, class I, function unknown; Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides; Belongs to the ribonucleoside diphosphate reductase small chain family. (320 aa) |
| | nrdE | Ribonucleoside-diphosphate reductase (major subunit); Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. (719 aa) |
| | ung | DNA-uracil glycosylase; Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. (222 aa) |
| | mutX | 8-oxodGTP nucleoside triphosphatase; Involved in the DNA repair system to avoid A.T to G.C transversions. Degrades 8-oxo-dGTP to the monophosphate, but is also active on all of the nucleoside triphosphates (By similarity). Belongs to the Nudix hydrolase family. (154 aa) |
| | xerC | Integrase/recombinase; Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. Required for resolution of chromosome dimers frequently formed by transformation ; Belongs to the 'phage' integrase family. XerS subfamily. (356 aa) |
| | rnh | Ribonuclease H; Endonuclease that specifically degrades the RNA of RNA-DNA hybrids; Belongs to the RNase HII family. (259 aa) |
| | rexA | First subunit of major exonuclease; The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities; Belongs to the helicase family. AddA subfamily. (1216 aa) |
| | rexB | Second subunit of major exonuclease; The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. This subunit has 5' -> 3' nuclease activity; Belongs to the helicase family. AddB/RexB type 2 subfamily. (1091 aa) |
| | ligA | DNA ligase; DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. (652 aa) |
| | rluD-2 | 23S rRNA pseudouridine synthase; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (298 aa) |
| | pcrA | ATP-dependent DNA helicase. (763 aa) |
| | IS1381-3 | Tranposase (orf2). (129 aa) |
| | rpoD | RNA polymerase sigmA FACTOR 70; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. (369 aa) |
| | dnaG | DNA primase; RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. (591 aa) |
| | spr0967 | Conserved hypothetical protein. (407 aa) |
| | ccrB | Low similarity to site-specific recombinase. (559 aa) |
| | spr0932 | Conserved hypothetical protein; Similar to RNA methyltransferase; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. (543 aa) |
| | tdk | Thymidine kinase. (199 aa) |
| | thdF | Thiophene and furan oxidation protein; Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. (479 aa) |
| | dnaQ | DNA polymerase III, epsilon chain. (208 aa) |
| | ebsC | Regulatory protein; Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily. (160 aa) |
| | spr0885 | Degenerate transposase (orf2). (80 aa) |
| | spr0883 | Conserved hypothetical protein; Probable methyltransferase. (237 aa) |
| | rnr | Exoribonuclease R; 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs. (784 aa) |
| | mutM | Formamidopyrimidine-DNA glycosylase; Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. (274 aa) |
| | ybeY | Conserved hypothetical protein; Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. (165 aa) |
| | celA | Competence induced; comE-like protein A also known as CilE; Putative DNA uptake by homology. (216 aa) |
| | gidA-2 | Glucose inhibited division protein; Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs; Belongs to the MnmG family. TrmFO subfamily. (444 aa) |
| | rsmI | Conserved hypothetical protein; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. (289 aa) |
| | spr0837 | Conserved hypothetical protein; Involved in initiation control of chromosome replication. Belongs to the YabA family. (114 aa) |
| | holB | DNA polymerase III, delta' subunit. (296 aa) |
| | rluD | 23S rRNA pseudouridine synthase (supresses ftsH(ts) mutants); Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (295 aa) |
| | spr0818 | Degenerate transposase. (175 aa) |
| | spr0815 | Degenerate transposase. (306 aa) |
| | dnaE | DNA-polymerase III alpha-chain. (1042 aa) |
| | hsdR-2 | Type 1 restriction modification system endonuclease R. (1116 aa) |
| | hsdS-4 | Type I restriction-modification enzyme 1, S subunit. (203 aa) |
| | hsdM-2 | Type I restriction modification enzyme methylase subunit. (497 aa) |
| | thiI | Thiamin biosynthesis protein; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. (404 aa) |
| | dnaX | DNA-directed DNA polymerase III chain; DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. (551 aa) |
| | parC | Topoisomerase IV subunit A; Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule; Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily. (826 aa) |
| | parE | Topoisomerase IV subunit B; Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule; Belongs to the type II topoisomerase family. ParE type 2 subfamily. (647 aa) |
| | spr0754 | Degenerate transposase (orf2). (162 aa) |
| | gyrB | DNA gyrase; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (648 aa) |
| | gph-2 | Phosphoglycolate phosphatase. (190 aa) |
| | dinG | ATP-dependent DNA helicase; 3'-5' exonuclease. (816 aa) |
| | metS | Methionyl-tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation; Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. (679 aa) |
| | trmD | tRNA (guanine-N1)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (239 aa) |
| | rimM | 16S rRNA processing protein RimM; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (172 aa) |
| | rlmN | Conserved hypothetical protein; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; Belongs to the radical SAM superfamily. RlmN family. (361 aa) |
| | holA | DNA polymerase III, delta subunit. (345 aa) |
| | rheB | ATP-dependent RNA helicase; Probable DEAD-box RNA helicase. May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures. (447 aa) |
| | lysS | Lysyl-tRNA synthetase (lysine--tRNA ligase) (LYSRS); Belongs to the class-II aminoacyl-tRNA synthetase family. (496 aa) |
| | spr0608 | Hypothetical protein. (337 aa) |
| | rsuA-2 | Ribosomal small subunit pseudouridine synthase A; Belongs to the pseudouridine synthase RsuA family. (241 aa) |
| | spr0595 | Conserved hypothetical protein. (126 aa) |
| | rnz | Conserved hypothetical protein; Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA; Belongs to the RNase Z family. (309 aa) |
| | miaA | tRNA isopentenylpyrophosphate transferase; Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A); Belongs to the IPP transferase family. (311 aa) |
| | rplA | 50S Ribosomal protein L1; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (229 aa) |
| | spr0550 | Conserved hypothetical protein. (44 aa) |
| | spr0549 | Hypothetical protein. (50 aa) |
| | uvrC | Exonuclease ABC - subunit C; The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision. (614 aa) |
| | rnj-2 | Conserved hypothetical protein; An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay. (553 aa) |
| | recJ | Single-stranded DNA-specific exonuclease, 5'-3'. (744 aa) |
| | mrnC | Conserved hypothetical protein; Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors; Belongs to the MrnC RNase family. (128 aa) |
| | cysS | Cysteinyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (447 aa) |
| | pnpA | Polyribonucleotide nucleotidyltransferase; Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction. (775 aa) |
| | pheT | Phenylalanyl-tRNA synthetase beta chain; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (800 aa) |
| | pheS | Phenylalanyl-tRNA synthetase alpha chain; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (375 aa) |
| | spr0496 | Degenerate transposase (orf2). (129 aa) |
| | spr0495 | Degenerate transposase (orf2). (35 aa) |
| | valS | Valyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (883 aa) |
| | rbfA | Ribosome binding factor A; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (125 aa) |
| | nusA | Transcription termination; Participates in both transcription termination and antitermination. (378 aa) |
| | trmB | Conserved hypothetical protein; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. (211 aa) |
| | dnaJ | Heat-shock protein (activation of DnaK); Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interaction [...] (372 aa) |
| | hsdR | EcoA type I restriction-modification enzyme R subunit. (777 aa) |
| | hsdM | EcoE type I restriction modification enzyme M subunit. (487 aa) |
| | hsdS-3 | Type I site-specific deoxyribonuclease chain S. (522 aa) |
| | xerD | Integrase/recombinase; Belongs to the 'phage' integrase family. (265 aa) |
| | hsdS-2 | Type I restriction enzyme EcoKI specificity protein (S protein). (199 aa) |
| | hsdS | Type I restriction enzyme. (426 aa) |
| | rpoE | RNA polymerase (delta subunit); Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling; Belongs to the RpoE family. (200 aa) |
| | cspR | rRNA methylase; Could methylate the ribose at the nucleotide 34 wobble position in tRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily. (180 aa) |
| | dinP | DNA-damage-inducible protein P; Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII. (356 aa) |
| | spr0412 | Degenerate transposase. (182 aa) |
| | gatC | Glutamyl tRNA-Gln amidotransferase, subunit C; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity); Belongs to the GatC family. (100 aa) |
| | gatB | Glutamyl tRNA-Gln amidotransferase subunit B; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln); Belongs to the GatB/GatE family. GatB subfamily. (480 aa) |
| | nusB | Transcription termination protein; Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. (146 aa) |
| | serS | Seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (445 aa) |
| | mutS2 | DNA mismatch repair protein; Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity; Belongs to the DNA mismatch repair MutS family. MutS2 subfamily. (778 aa) |
| | rnhB | Ribonuclease HII; Endonuclease that specifically degrades the RNA of RNA-DNA hybrids; Belongs to the RNase HII family. RnhC subfamily. (290 aa) |
| | recD | Exonuclease V; DNA-dependent ATPase and ATP-dependent 5'-3' DNA helicase. Has no activity on blunt DNA or DNA with 3'-overhangs, requires at least 10 bases of 5'-ssDNA for helicase activity; Belongs to the RecD family. RecD-like subfamily. (788 aa) |
| | spr0353 | Degenerate transposase (orf2). (117 aa) |
| | spr0352 | Degenerate transposase (orf2). (61 aa) |
| | spr0333 | Conserved hypothetical protein; Belongs to the methyltransferase superfamily. (390 aa) |
| | recU | Recombination protein U; Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation (By similarity). Belongs to the RecU family. (198 aa) |
| | cps2A | The type 2 capsule locus of Streptococcus pneumoniae. (320 aa) |
| | yllC | YllC protein; Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. (316 aa) |
| | rsuA | Ribosomal small subunit pseudouridine synthase A; Belongs to the pseudouridine synthase RsuA family. (240 aa) |
| | spr0252 | Hypothetical protein. (87 aa) |
| | polC | DNA polymerase III, alpha subunit; Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity. (1463 aa) |
| | proS | Prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (617 aa) |
| | ruvB | Branch migration of Holliday structures; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. (332 aa) |
| | leuS | Leucyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (833 aa) |
| | spr0217 | Conserved hypothetical protein; ACT domain containing protein. (119 aa) |
| | rpoA | RNA polymerase alpha subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (311 aa) |
| | rpsK | 30S Ribosomal protein S11; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (127 aa) |
| | nrdD | Ribonucleotide reductase, class III, anaerobic. (737 aa) |
| | spr0176 | Conserved hypothetical protein; Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA. (139 aa) |
| | uvrA | Excinuclease ABC - subunit A; The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate. (943 aa) |
| | tag | 3-Methyladenine DNA glycosylase I, constitutive. (187 aa) |
| | ruvA | Holliday junction DNA helicase; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. (197 aa) |
| | hexB | DNA mismatch repair protein; This protein is involved in the repair of mismatches in DNA. The hex system is nick-directed. (649 aa) |
| | gcp | Secreted metalloendopeptidase Gcp; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction; Belongs to the KAE1 / TsaD family. (336 aa) |
| | spr0129 | Conserved hypothetical protein. (227 aa) |
| | spr0126 | Conserved hypothetical protein; Belongs to the UPF0356 family. (77 aa) |
| | rnj | Conserved hypothetical protein; An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay. (610 aa) |
| | gidA | Glucose inhibited division protein A; NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34; Belongs to the MnmG family. (637 aa) |
| | trmU | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. (391 aa) |
| | spr0084 | Conserved hypothetical protein; Belongs to the UPF0176 family. (328 aa) |
| | recO | Conserved hypothetical protein; Involved in DNA repair and RecF pathway recombination (By similarity). Plays no role in chromosomal or plasmid transformation but is required for resolution of chromosome dimers occurring as intermediates in the formation of merodiploids by transformation ; Belongs to the RecO family. (256 aa) |
| | polA | DNA polymerase I; In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. (877 aa) |
| | radA | DNA repair: sensitivity to gamma and UV radiation; Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks (By similarity). Required for efficient transformation with chromosomal (linear) DNA, but not for replicative plasmid DNA. Its increased sensitivity to a DNA damaging agent suggests it may be required for DNA repair. (420 aa) |
| | tadA | Conserved hypothetical protein; Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2); Belongs to the cytidine and deoxycytidylate deaminase family. (155 aa) |
| | comX1 | Competence-specific global transcription modulator; Putative sigma factor. (159 aa) |
| | tilS | Conserved hypothetical protein; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Belongs to the tRNA(Ile)-lysidine synthase family. (425 aa) |
| | mfd | Transcription-repair coupling factor; Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site; In the C-terminal section; belongs to the helicase family. RecG subfamily. (1169 aa) |
| | dnaN | DNA biosynthesis; Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as [...] (378 aa) |
| | dnaA | DNA biosynthesis, initiation, binding protein; Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'- TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. (453 aa) |
