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leuA | 2-ISOPROPYLMALATE SYNTHASE; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. (616 aa) | ||||
metX | Homoserine O-Acetyltransferase; Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine; Belongs to the AB hydrolase superfamily. MetX family. (377 aa) | ||||
metY | O-Acetylhomoserine (Thiol)-Lyase; Catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway (Ref.4,. Can also use dimethyldisulfide and methanethiol as reduced sulfur sources, leading to the direct formation of methionine. Has weak cystathionine gamma-synthase activity. (437 aa) | ||||
sahH | Adenosylhomocysteinase; May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine. (478 aa) | ||||
serC | Phosphoserine Transaminase; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine. (376 aa) | ||||
Cgl0839 | Alpha/beta hydrolase fold; Belongs to the AB hydrolase superfamily. MetX family. (349 aa) | ||||
tdcB | THREONINE DEHYDRATASE. (310 aa) | ||||
glyA | Serine Hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (434 aa) | ||||
Cgl0998 | Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase. (241 aa) | ||||
metE | Homocysteine methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. (745 aa) | ||||
hom | Homoserine Dehydrogenase; Belongs to the homoserine dehydrogenase family. (445 aa) | ||||
thrB | Homoserine Kinase; Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate; Belongs to the GHMP kinase family. Homoserine kinase subfamily. (309 aa) | ||||
ilvD | DIHYDROXY-ACID DEHYDRATASE; Belongs to the IlvD/Edd family. (613 aa) | ||||
ilvB | ACETOLACTATE SYNTHASE. (626 aa) | ||||
ilvN | ACETOHYDROXYACID SYNTHASE SMALL SUBUNIT. (172 aa) | ||||
ilvC | KETOL-ACID REDUCTOISOMERASE; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. (338 aa) | ||||
leuB | 3-ISOPROPYLMALATE DEHYDROGENASE; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity); Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily. (340 aa) | ||||
leuC | 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily. (481 aa) | ||||
leuD | 3-ISOPROPYLMALATE DEHYDRATASE (SMALL SUBUNIT); Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (197 aa) | ||||
metH | Homocysteine Methyltransferase; Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. (1196 aa) | ||||
metK | S-Adenosylmethionine Synthetase; Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. (407 aa) | ||||
sdaA | PROBABLE L-SERINE DEHYDRATASE (L-SERINE DEAMINASE); Belongs to the iron-sulfur dependent L-serine dehydratase family. (449 aa) | ||||
ilvA | THREONINE DEHYDRATASE; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity). (436 aa) | ||||
Cgl2129 | Similar to methionine synthase II (cobalamin-independent). (405 aa) | ||||
metF | 5,10-Methylenetetrahydrofolate Reductase; Belongs to the methylenetetrahydrofolate reductase family. (326 aa) | ||||
ilvE | BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE. (379 aa) | ||||
thrC | THREONINE SYNTHASE; Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine. (481 aa) | ||||
aecD | Cystathionine beta-Lyase. (368 aa) | ||||
metB | Cystathionine gamma-Synthase. (386 aa) | ||||
serB | Phosphoserine Phosphatase. (446 aa) | ||||
cysK | O-Acetylserine (Thiol)-Lyase; Belongs to the cysteine synthase/cystathionine beta- synthase family. (311 aa) | ||||
cysE | Serine O-Acetyltransferase. (188 aa) | ||||
cysS | CYSTEINE TRNA SYNTHETASE; Belongs to the class-I aminoacyl-tRNA synthetase family. (460 aa) | ||||
Cgl2786 | Putative L,L-Cystathionine gamma-Lyase. (382 aa) |