Your Input: | |
| | ilvD | Dihydroxy-acid dehydratase; Escherichia coli K-12 ortholog: b3771; Escherichia coli O157:H7 ortholog: z5282. (616 aa) |
| | thrA | Bifunctional; Escherichia coli K-12 ortholog: b0002; Escherichia coli O157:H7 ortholog: z0002; bifunctional; Homoserine dehydrogenase I; In the C-terminal section; belongs to the homoserine dehydrogenase family. (841 aa) |
| | thrB | Homoserine kinase; Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate; Belongs to the GHMP kinase family. Homoserine kinase subfamily. (310 aa) |
| | thrC | Threonine synthase; Escherichia coli K-12 ortholog: b0004; Escherichia coli O157:H7 ortholog: z0004. (428 aa) |
| | dapB | Dihydrodipicolinate reductase; Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate; Belongs to the DapB family. (273 aa) |
| | carA | Carbamoyl-phosphate synthase small chain; Escherichia coli K-12 ortholog: b0032; Escherichia coli O157:H7 ortholog: z0037; Belongs to the CarA family. (391 aa) |
| | carB | Carbamoyl-phosphate synthase large chain; Escherichia coli K-12 ortholog: b0033; Escherichia coli O157:H7 ortholog: z0038. (1073 aa) |
| | folA | Dihydrofolate reductase; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity). (204 aa) |
| | leuD | 3-isopropylmalate dehydratase small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. (201 aa) |
| | leuC | 3-isopropylmalate dehydratase large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (466 aa) |
| | leuB | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. (364 aa) |
| | leuA | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. (537 aa) |
| | ilvI | Acetolactate synthase isozyme III large subunit; Escherichia coli K-12 ortholog: b0077; Escherichia coli O157:H7 ortholog: z0087. (604 aa) |
| | ilvH | Acetolactate synthase isozyme III small subunit; Escherichia coli K-12 ortholog: b0078; Escherichia coli O157:H7 ortholog: z0088. (173 aa) |
| | panD | Aspartate 1-decarboxylase precursor; Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine. (126 aa) |
| | pfs | MTA/SAH nucleosidase; Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'- methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation. Belongs to the PNP/UDP phosphorylas [...] (232 aa) |
| | dapD | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase; Escherichia coli K-12 ortholog: b0166; Escherichia coli O157:H7 ortholog: z0176; Belongs to the transferase hexapeptide repeat family. (274 aa) |
| | proB | Glutamate 5-kinase; Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate. (380 aa) |
| | proA | Gamma-glutamyl phosphate reductase; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. Belongs to the gamma-glutamyl phosphate reductase family. (417 aa) |
| | c0444 | Carbamate kinase-like protein yahI; Escherichia coli K-12 ortholog: b0323; Escherichia coli O157:H7 ortholog: z0412; Belongs to the carbamate kinase family. (316 aa) |
| | proC | Pyrroline-5-carboxylate reductase; Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. (305 aa) |
| | ybaS | Probable glutaminase ybaS; Escherichia coli K-12 ortholog: b0485; Escherichia coli O157:H7 ortholog: z0606; Belongs to the glutaminase family. (310 aa) |
| | arcC | Carbamate kinase; Escherichia coli K-12 ortholog: b0521; Escherichia coli O157:H7 ortholog: z0676; Belongs to the carbamate kinase family. (297 aa) |
| | folD | FolD bifunctional protein; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. (288 aa) |
| | asnB | Asparagine synthetase B (glutamine-hydrolyzing); Escherichia coli K-12 ortholog: b0674; Escherichia coli O157:H7 ortholog: z0821. (632 aa) |
| | sucB | Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2- oxoglutarate to succinyl-CoA and CO(2). (405 aa) |
| | ybiB | Hypothetical protein ybiB; Escherichia coli K-12 ortholog: b0800; Escherichia coli O157:H7 ortholog: z1021. (320 aa) |
| | serS | Seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (430 aa) |
| | serC | Phosphoserine aminotransferase; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. (362 aa) |
| | aspC | Aspartate aminotransferase; Escherichia coli K-12 ortholog: b0928; Escherichia coli O157:H7 ortholog: z1275. (396 aa) |
| | putA | PutA protein; Oxidizes proline to glutamate for use as a carbon and nitrogen source; Belongs to the aldehyde dehydrogenase family. In the N-terminal section; belongs to the proline dehydrogenase family. (1342 aa) |
| | dadA | D-amino acid dehydrogenase small subunit; Oxidative deamination of D-amino acids; Belongs to the DadA oxidoreductase family. (434 aa) |
| | dadX | Alanine racemase, catabolic; Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA. (356 aa) |
| | trpA | Tryptophan synthase alpha chain; The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. Belongs to the TrpA family. (268 aa) |
| | trpB | Tryptophan synthase beta chain; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. (397 aa) |
| | trpD | Anthranilate synthase component II; Catalyzes the transfer of the phosphoribosyl group of 5- phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'- phosphoribosyl)-anthranilate (PRA). (531 aa) |
| | trpE | Anthranilate synthase component I; Escherichia coli K-12 ortholog: b1264; Escherichia coli O157:H7 ortholog: z2546. (520 aa) |
| | cysB | Cys regulon transcriptional activator; This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for CysB is N-acetylserine (By similarity). (324 aa) |
| | ydcW | Putative betaine aldehyde dehydrogenase; Catalyzes the oxidation 4-aminobutanal (gamma- aminobutyraldehyde) to 4-aminobutanoate (gamma-aminobutyrate or GABA). This is the second step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate via 4- aminobutanal. Also functions as a 5-aminopentanal dehydrogenase in a a L-lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. (474 aa) |
| | gadB | Glutamate decarboxylase beta; Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria (By similarity). (489 aa) |
| | yneH | Probable glutaminase yneH; Escherichia coli K-12 ortholog: b1524; Escherichia coli O157:H7 ortholog: z2179; Belongs to the glutaminase family. (308 aa) |
| | sufS | Selenocysteine lyase; Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L- selenocysteine. Selenocysteine lyase activity is however unsure in vivo. (406 aa) |
| | ydjS | Succinylglutamate desuccinylase; Transforms N(2)-succinylglutamate into succinate and glutamate; Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily. (322 aa) |
| | astB | Succinylarginine dihydrolase; Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)- succinylornithine, ammonia and CO(2). (447 aa) |
| | astD | Succinylglutamic semialdehyde dehydrogenase; Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate. (492 aa) |
| | astA | Arginine N-succinyltransferase; Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine. (315 aa) |
| | cstC | Succinylornithine transaminase; Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. AstC subfamily. (406 aa) |
| | selD | Selenide,water dikinase; Synthesizes selenophosphate from selenide and ATP. (349 aa) |
| | yedO | Putative 1-aminocyclopropane-1-carboxylate deaminase; Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine; Belongs to the ACC deaminase/D-cysteine desulfhydrase family. (328 aa) |
| | hisH | Imidazole glycerol phosphate synthase subunit hisH; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF (By similarity). (196 aa) |
| | wcaB | Putative colanic acid biosynthesis acetyltransferase wcaB; Escherichia coli K-12 ortholog: b2058; Escherichia coli O157:H7 ortholog: z3222; Belongs to the transferase hexapeptide repeat family. (162 aa) |
| | alaA | Probable aminotransferase yfbQ; Involved in the biosynthesis of alanine. (405 aa) |
| | purF | Amidophosphoribosyltransferase; Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine; In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. (505 aa) |
| | dsdA | D-serine dehydratase; Escherichia coli K-12 ortholog: b2366; Escherichia coli O157:H7 ortholog: z3628. (442 aa) |
| | cysZ | CysZ protein; High affinity, high specificity proton-dependent sulfate transporter, which mediates sulfate uptake. Provides the sulfur source for the cysteine synthesis pathway; Belongs to the CysZ family. (253 aa) |
| | cysK | Cysteine synthase A; Escherichia coli K-12 ortholog: b2414; Escherichia coli O157:H7 ortholog: z3680; Belongs to the cysteine synthase/cystathionine beta- synthase family. (327 aa) |
| | cysM | Cysteine synthase B; Escherichia coli K-12 ortholog: b2421; Escherichia coli O157:H7 ortholog: z3686; Belongs to the cysteine synthase/cystathionine beta- synthase family. (303 aa) |
| | dapE | Succinyl-diaminopimelate desuccinylase; Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls; Belongs to the peptidase M20A family. DapE subfamily. (375 aa) |
| | dapA | Dihydrodipicolinate synthase; Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). (298 aa) |
| | guaA | GMP synthase (glutamine-hydrolyzing); Catalyzes the synthesis of GMP from XMP. (525 aa) |
| | glyA | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (419 aa) |
| | purL | Phosphoribosylformylglycinamidine synthase; Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. (1295 aa) |
| | pheA | P-protein; Escherichia coli K-12 ortholog: b2599; Escherichia coli O157:H7 ortholog: z3891; bifunctional; Chorismate mutase; Prephenate dehydratase. (386 aa) |
| | tyrA | T-protein; Escherichia coli K-12 ortholog: b2600; Escherichia coli O157:H7 ortholog: z3892; bifunctional; Chorismate mutase; Prephenate dehydrogenase. (373 aa) |
| | ygaT | Hypothetical protein ygaT; Acts as an alpha-ketoglutarate-dependent dioxygenase catalyzing hydroxylation of glutarate (GA) to L-2-hydroxyglutarate (L2HG). Functions in a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. (325 aa) |
| | ygaF | Hypothetical protein ygaF; Catalyzes the dehydrogenation of L-2-hydroxyglutarate (L2HG) to alpha-ketoglutarate and couples to the respiratory chain by feeding electrons from the reaction into the membrane quinone pool. Functions in a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. Reaction=(S)-2-hydroxyglutarate + a quinone = 2-oxoglutarate + a quinol; Xref=Rhea:RHEA:58664, ChEBI:CHEBI:16782, ChEBI:CHEBI:16810, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:58665; Belongs to the L2HGDH family. (444 aa) |
| | cysI | Sulfite reductase [NADPH] hemoprotein beta-component; Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. (570 aa) |
| | cysJ | Sulfite reductase [NADPH] flavoprotein alpha-component; Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component; Belongs to the NADPH-dependent sulphite reductase flavoprotein subunit CysJ family. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. (599 aa) |
| | pyrG | CTP synthase; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates. (545 aa) |
| | c3381 | Cysteine sulfinate desulfinase; Escherichia coli K-12 ortholog: b2810; Escherichia coli O157:H7 ortholog: z4127. (413 aa) |
| | argA | Amino-acid acetyltransferase; Escherichia coli K-12 ortholog: b2818; Escherichia coli O157:H7 ortholog: z4135; Belongs to the acetyltransferase family. ArgA subfamily. (465 aa) |
| | lysA | Diaminopimelate decarboxylase; Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine. (420 aa) |
| | yqeA | Carbamate kinase-like protein yqeA; Escherichia coli K-12 ortholog: b2874; Escherichia coli O157:H7 ortholog: z4213; Belongs to the carbamate kinase family. (310 aa) |
| | gcvP | Glycine dehydrogenase (decarboxylating); The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein; Belongs to the GcvP family. (957 aa) |
| | gcvH | Glycine cleavage system H protein; The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein. (130 aa) |
| | gcvT | Aminomethyltransferase; The glycine cleavage system catalyzes the degradation of glycine. (364 aa) |
| | serA | D-3-phosphoglycerate dehydrogenase; Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L- serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate; Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. (410 aa) |
| | speB | Agmatinase; Catalyzes the formation of putrescine from agmatine. Belongs to the arginase family. Agmatinase subfamily. (306 aa) |
| | speA | Biosynthetic arginine decarboxylase; Catalyzes the biosynthesis of agmatine from arginine. (662 aa) |
| | ansB | L-asparaginase II precursor; Escherichia coli K-12 ortholog: b2957; Escherichia coli O157:H7 ortholog: z4302; Belongs to the asparaginase 1 family. (348 aa) |
| | metC | Cystathionine beta-lyase; Escherichia coli K-12 ortholog: b3008; Escherichia coli O157:H7 ortholog: z4361. (440 aa) |
| | ygjG | Probable ornithine aminotransferase; Catalyzes the aminotransferase reaction from putrescine to 2- oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. This is the first step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate (gamma-aminobutyrate or GABA) via 4- aminobutanal. Also functions as a cadaverine transaminase in a a L- lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. (496 aa) |
| | yhaR | TdcF protein; May be a post-translational regulator that controls the metabolic fate of L-threonine or the potentially toxic intermediate 2- ketobutyrate. (150 aa) |
| | tdcD-2 | Putative conserved protein; Catalyzes the conversion of propionyl phosphate and ADP to propionate and ATP. (50 aa) |
| | tdcB | Threonine dehydratase catabolic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. TdcB also dehydrates serine to y [...] (329 aa) |
| | argG | Argininosuccinate synthase; Escherichia coli K-12 ortholog: b3172; Escherichia coli O157:H7 ortholog: z4534; Belongs to the argininosuccinate synthase family. Type 2 subfamily. (447 aa) |
| | gltB | Glutamate synthase [NADPH] large chain precursor; Escherichia coli K-12 ortholog: b3212; Escherichia coli O157:H7 ortholog: z4576. (1522 aa) |
| | argR | Arginine repressor; Regulates arginine biosynthesis genes; Belongs to the ArgR family. (156 aa) |
| | argD | Acetylornithine aminotransferase; Involved in both the arginine and lysine biosynthetic pathways. (406 aa) |
| | pabA | Para-aminobenzoate synthase glutamine amidotransferase component II; Escherichia coli K-12 ortholog: b3360; Escherichia coli O157:H7 ortholog: z4721. (187 aa) |
| | asd | Aspartate-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. (387 aa) |
| | gadA | Glutamate decarboxylase alpha; Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria (By similarity). (487 aa) |
| | cysE | Serine acetyltransferase; Escherichia coli K-12 ortholog: b3607; Escherichia coli O157:H7 ortholog: z5034; Belongs to the transferase hexapeptide repeat family. (273 aa) |
| | tdh | Threonine 3-dehydrogenase; Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2- amino-3-ketobutyrate; Belongs to the zinc-containing alcohol dehydrogenase family. (341 aa) |
| | kbl | 2-amino-3-ketobutyrate coenzyme A ligase; Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA. (398 aa) |
| | ilvN | Acetolactate synthase isozyme I small subunit; Escherichia coli K-12 ortholog: b3670; Escherichia coli O157:H7 ortholog: z5164; Belongs to the acetolactate synthase small subunit family. (96 aa) |
| | ilvB | Acetolactate synthase isozyme I large subunit; Escherichia coli K-12 ortholog: b3671; Escherichia coli O157:H7 ortholog: z5165. (562 aa) |
| | tnaA | Tryptophanase; Escherichia coli K-12 ortholog: b3708; Escherichia coli O157:H7 ortholog: z5203; Belongs to the beta-eliminating lyase family. (476 aa) |
| | glmS | Glucosamine--fructose-6-phosphate aminotransferase (isomerizing); Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source. (609 aa) |
| | asnA | Aspartate--ammonia ligase; Escherichia coli K-12 ortholog: b3744; Escherichia coli O157:H7 ortholog: z5245; Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. (330 aa) |
| | ilvG | Acetohydroxy acid synthase II; Escherichia coli O157:H7 ortholog: z5279. (548 aa) |
| | ilvM | Acetolactate synthase isozyme II small subunit; Escherichia coli K-12 ortholog: b3769; Escherichia coli O157:H7 ortholog: z5280. (87 aa) |
| | ilvE | Branched-chain amino acid aminotransferase; Acts on leucine, isoleucine and valine. (309 aa) |
| | ilvA | Threonine dehydratase biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. (515 aa) |
| | ilvC | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. (541 aa) |
| | dapF | Diaminopimelate epimerase; Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan. (275 aa) |
| | metR | Transcriptional activator protein metR; Escherichia coli K-12 ortholog: b3828; Escherichia coli O157:H7 ortholog: z5349; Belongs to the LysR transcriptional regulatory family. (317 aa) |
| | metE | 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. (753 aa) |
| | c4764 | Carbamate kinase-like protein yahI; Escherichia coli K-12 ortholog: b0323; Escherichia coli O157:H7 ortholog: z0412; Belongs to the carbamate kinase family. (318 aa) |
| | glnA | Glutamine synthetase; Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia; Belongs to the glutamine synthetase family. (469 aa) |
| | yihZ | D-tyrosyl-tRNA(Tyr) deacylase; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. Belongs to the DTD family. (145 aa) |
| | metJ | Met repressor; This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis; Belongs to the MetJ family. (151 aa) |
| | metB | Cystathionine gamma-synthase; Escherichia coli K-12 ortholog: b3939; Escherichia coli O157:H7 ortholog: z5494. (424 aa) |
| | metL | AKII-HDII protein; Bifunctional; Escherichia coli K-12 ortholog: b3940; Escherichia coli O157:H7 ortholog: z5495; bifunctional; Aspartokinase II; Homoserine dehydrogenase II; In the C-terminal section; belongs to the homoserine dehydrogenase family. (810 aa) |
| | metF | 5,10-methylenetetrahydrofolate reductase; Escherichia coli K-12 ortholog: b3941; Escherichia coli O157:H7 ortholog: z5496; Belongs to the methylenetetrahydrofolate reductase family. (296 aa) |
| | argE | Acetylornithine deacetylase; Displays a broad specificity and can also deacylate substrates such as acetylarginine, acetylhistidine or acetylglutamate semialdehyde. (396 aa) |
| | argC | N-acetyl-gamma-glutamyl-phosphate reductase; Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. (334 aa) |
| | argB | Acetylglutamate kinase; Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate. (269 aa) |
| | argH | Argininosuccinate lyase; Escherichia coli K-12 ortholog: b3960; Escherichia coli O157:H7 ortholog: z5518. (457 aa) |
| | metA | Homoserine O-succinyltransferase; Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine; Belongs to the MetA family. (309 aa) |
| | metH | 5-methyltetrahydrofolate--homocysteine methyltransferase; Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. (1227 aa) |
| | lysC | Lysine-sensitive aspartokinase III; Escherichia coli K-12 ortholog: b4024; Escherichia coli O157:H7 ortholog: z5622; Belongs to the aspartokinase family. (449 aa) |
| | alr | Alanine racemase, biosynthetic; Catalyzes the interconversion of L-alanine and D-alanine. Provides the D-alanine required for cell wall biosynthesis (By similarity). (359 aa) |
| | tyrB | Aromatic-amino-acid aminotransferase; Escherichia coli K-12 ortholog: b4054; Escherichia coli O157:H7 ortholog: z5652. (411 aa) |
| | c5034 | Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; Escherichia coli K-12 ortholog: b0727; Escherichia coli O157:H7 ortholog: z0881. (351 aa) |
| | aspA | Aspartate ammonia-lyase; Escherichia coli K-12 ortholog: b4139; Escherichia coli O157:H7 ortholog: z5744; Belongs to the class-II fumarase/aspartase family. Aspartase subfamily. (493 aa) |
| | yjgF | Protein yjgF; Accelerates the release of ammonia from reactive enamine/imine intermediates of the PLP-dependent threonine dehydratase (IlvA) in the low water environment of the cell. It catalyzes the deamination of enamine/imine intermediates to yield 2-ketobutyrate and ammonia. It is required for the detoxification of reactive intermediates of IlvA due to their highly nucleophilic abilities. Involved in the isoleucine biosynthesis (By similarity); Belongs to the RutC family. (152 aa) |
| | argR-2 | Hypothetical protein; Regulates arginine biosynthesis genes. (184 aa) |
| | arcB-2 | Ornithine carbamoyltransferase chain I; Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. (343 aa) |
| | c5349 | Carbamate kinase; Escherichia coli K-12 ortholog: b0521; Escherichia coli O157:H7 ortholog: z0676; Belongs to the carbamate kinase family. (310 aa) |
| | arcA-2 | Putative arginine deiminase; Residues 7 to 407 of 407 are 47.79 pct identical to residues 10 to 416 of 418 from SwissProt.40 : >sp|P13981|ARCA_PSEAE Arginine deiminase (ADI) (Arginine dihydrolase) (AD). (407 aa) |
| | argI | Ornithine carbamoyltransferase chain I; Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. (334 aa) |
| | serB | Phosphoserine phosphatase; Escherichia coli K-12 ortholog: b4388; Escherichia coli O157:H7 ortholog: z5989. (368 aa) |
| | thrL | Thr operon leader peptide; This protein is involved in control of the biosynthesis of threonine. (21 aa) |
| | leuL | Leu operon leader peptide; Involved in control of the biosynthesis of leucine. (28 aa) |
| | trpL | Trp operon leader peptide; This protein is involved in control of the biosynthesis of tryptophan. (14 aa) |
