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PA0122 PA0122 impA impA toxR toxR cbpD cbpD pqsA pqsA pqsB pqsB pqsC pqsC pqsD pqsD pqsE pqsE phnA phnA mvfR mvfR rhlC rhlC PA1131 PA1131 toxA toxA pcpS pcpS lasR lasR rsaL rsaL lasI lasI PA1469 PA1469 acpP2 acpP2 lasA lasA qscR qscR phzA2 phzA2 phzB2 phzB2 phzC2 phzC2 phzD2 phzD2 phzE2 phzE2 phzF2 phzF2 phzG2 phzG2 pvcA pvcA pvcB pvcB pvcC pvcC pvcD pvcD ptxR ptxR chiC chiC PA2301 PA2301 ambE ambE ambD ambD ambC ambC ambB ambB ambA ambA lecA lecA pqsH pqsH qteE qteE lap lap apeB apeB PA3327 PA3327 PA3328 PA3328 PA3329 PA3329 PA3330 PA3330 PA3331 PA3331 PA3332 PA3332 PA3334 PA3334 PA3335 PA3335 PA3336 PA3336 lecB lecB rhlI rhlI rhlR rhlR rhlB rhlB rhlA rhlA lasB lasB rhl rhl PA4078 PA4078 PA4079 PA4079 piv piv pqsL pqsL phzM phzM phzA1 phzA1 phzB1 phzB1 phzC1 phzC1 phzD1 phzD1 phzF1 phzF1 phzS phzS PA4907 PA4907 PA5220 PA5220
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splice isoforms or post-translational modifications are collapsed, i.e. each node represents all the proteins produced by a single, protein-coding gene locus.
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query proteins and first shell of interactors
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second shell of interactors
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proteins of unknown 3D structure
filled nodes:
a 3D structure is known or predicted
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Known Interactions
from curated databases
experimentally determined
Predicted Interactions
gene neighborhood
gene fusions
gene co-occurrence
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textmining
co-expression
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PA0122Conserved hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (136 aa)
impAHypothetical protein; Protease that degrades several proteins of the host immune system. Cleaves P-selectin glycoprotein ligand-1 (PSGL-1), leading to its functional inhibition; PSGL-1 is a leukocyte cell-surface receptor essential for leukocyte recruitment to the site of infection. Next to PSGL-1, targets host CD43 and CD44 that are also involved in leukocyte homing. Thus, prevents neutrophil extravasation and thereby protects P.aeruginosa from neutrophil attack. Is also able to inhibit the decay accelerating factor (CD55), but not the cell-surface receptors CD46 and CD31; Belongs to [...] (923 aa)
toxRTranscriptional regulator ToxR; Positive regulation of toxA gene transcription. (259 aa)
cbpDChitin-binding protein CbpD precursor; Binds chitin but does not hydrolyze it, has no detectable protease or staphylolytic activity. (389 aa)
pqsAProbable coenzyme A ligase; Catalyzes the formation of anthraniloyl-CoA, which is the priming step for entry into the Pseudomonas quinolone signal (PQS) biosynthetic pathway. Also active on a variety of aromatic substrates, including benzoate and chloro and fluoro derivatives of anthranilate. (517 aa)
pqsBPqsB; Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)- quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. The PqsC/PqsB complex catalyzes the condensation of 2-aminobenzoylacetate (2-ABA) and octanoyl-CoA to form HHQ. PqsB, together with PqsC, catalyzes the coupling of 2-ABA with the octanoate group, leading to decarboxylation and dehydration, and resulting in closure of the quinoline ring. PqsB is probably required for the proper folding of PqsC ra [...] (283 aa)
pqsCPqsC; Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)- quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. The PqsC/PqsB complex catalyzes the condensation of 2-aminobenzoylacetate (2-ABA) and octanoyl-CoA to form HHQ. First, PqsC acquires an octanoyl group from octanoyl-CoA and forms an octanoyl-PqsC intermediate. Then, together with PqsB, it catalyzes the coupling of 2-ABA with the octanoate group, leading to decarboxylation and dehydration, and re [...] (348 aa)
pqsD3-oxoacyl-[acyl-carrier-protein] synthase III; Required for the biosynthesis of a number of signaling molecules, such as the quinolone signal 2-heptyl-3-hydroxy-4(1H)- quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ) and 2,4- dihydroxyquinoline (DHQ). These molecules are required for normal biofilm formation. Catalyzes the transfer of the anthraniloyl moiety from anthraniloyl-CoA to malonyl-CoA to form 2-aminobenzoylacetyl-CoA. The first step of the reaction is the formation of a covalent anthraniloyl-PqsD intermediate. Next, the short-lived intermediate 3-(2-aminophenyl)- 3-oxopropa [...] (337 aa)
pqsEQuinolone signal response protein; Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)- quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. Catalyzes the hydrolysis of the intermediate 2-aminobenzoylacetyl-CoA (2-ABA-CoA) to form 2- aminobenzoylacetate (2-ABA), the precursor of HHQ. In vitro, can also hydrolyze other substrates such as S-ethyl-acetothioacetate and acetoacetyl-CoA, but is inactive against anthraniloyl-CoA, malonyl-CoA and octanoyl-CoA. Be [...] (301 aa)
phnAAnthranilate synthase component I; Part of a heterotetrameric complex that catalyzes the two- step biosynthesis of anthranilate, a precursor for Pseudomonas quinolone signal (2-heptyl-3-hydroxy-4-quinolone; PQS) production which is required to induce the genes for the biosynthesis of the virulence factor pyocyanine (PCN), a characteristic blue-green phenazine pigment produced by P.aeruginosa. In the first step, the glutamine-binding beta subunit (PhnB) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with [...] (530 aa)
mvfRTranscriptional regulator MvfR; Transcription regulator that plays a critical role in virulence by positively regulating the expression of multiple quorum sensing (QS)-regulated virulence factors, genes involved in protein secretion, translation, response to oxidative stress and the phnAB operon. At the stationary phase, negatively autoregulates its function through cleavage and translocation to the extracellular space ; Belongs to the LysR transcriptional regulatory family. (332 aa)
rhlCRhamnosyltransferase 2; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (325 aa)
PA1131Probable major facilitator superfamily (MFS) transporter; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (422 aa)
toxAExotoxin A precursor; An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38. (638 aa)
pcpSPcpS; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa); Belongs to the P-Pant transferase superfamily. (242 aa)
lasRTranscriptional regulator LasR; Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer; Belongs to the autoinducer-regulated transcriptional regulatory protein family. (239 aa)
rsaLRegulatory protein RsaL; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (80 aa)
lasIAutoinducer synthesis protein LasI; Required for the synthesis of PAI consisting of 3-oxo-N- (tetrahydro-2-oxo-3-furanyl)-dodecanamide also known as N-(3- oxododecanoyl)homoserine lactone, an autoinducer molecule which binds to LasR and thus acts in elastase biosynthesis regulation. (201 aa)
PA1469Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (240 aa)
acpP2Probable acyl carrier protein; Carrier of the growing fatty acid chain in fatty acid biosynthesis; Belongs to the acyl carrier protein (ACP) family. (79 aa)
lasALasA protease precursor; Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly- Gly-|-X substrates where X is Ala or Gly. Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases. Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a [...] (418 aa)
qscRQuorum-sensing control repressor; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (237 aa)
phzA2Probable phenazine biosynthesis protein; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule having important roles in virulence, competition and biological control. PhzA2 (operon phzA2B2C2E2F2G2) has a role in the biosynthesis of the phenazine during both planktonic growth and biofilm development, and in host infection during biofilm development. (162 aa)
phzB2Probable phenazine biosynthesis protein; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule having important roles in virulence, competition and biological control. PhzB2 (operon phzA2B2C2E2F2G2) has a role in the biosynthesis of the phenazine during both planktonic growth and biofilm development, and in host infection during biofilm development; Belongs to the PhzA/PhzB family. (162 aa)
phzC2Phenazine biosynthesis protein PhzC; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (405 aa)
phzD2Phenazine biosynthesis protein PhzD; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule. PhzD1 (operon phzA1B1C1E1F1G1) has a role in the biosynthesis of the phenazine during planktonic growth. Catalyzes the hydrolysis of the vinyl ether functional group of 2-amino-2-deoxyisochorismate (ADIC), yielding pyruvate and trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA). Also able to act on isochorismate, chorismate and 4-amino-4-deoxychorismate (ADC) as substrates ; Belongs to the isochorismatase family. (207 aa)
phzE2Phenazine biosynthesis protein PhzE; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (627 aa)
phzF2Probable phenazine biosynthesis protein; Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known. (278 aa)
phzG2Probable pyridoxamine 5'-phosphate oxidase; Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). (215 aa)
pvcAPaerucumarin biosynthesis protein PvcA; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (328 aa)
pvcBPaerucumarin biosynthesis protein PvcB; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (291 aa)
pvcCPaerucumarin biosynthesis protein PvcC; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (500 aa)
pvcDPaerucumarin biosynthesis protein PvcD; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (215 aa)
ptxRTranscriptional regulator PtxR; Transcriptional activator of the toxA (exotoxin) and regA genes. (312 aa)
chiCChitinase; Product name confidence: Class 2 (High similarity to functionally studied protein); Belongs to the glycosyl hydrolase 18 family. (483 aa)
PA2301Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (180 aa)
ambEAmbE; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (2124 aa)
ambDAmbD; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (339 aa)
ambCAmbC; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (362 aa)
ambBAmbB; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa; Belongs to the ATP-dependent AMP-binding enzyme family. (1249 aa)
ambAAmbA; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (205 aa)
lecALecA; D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl- beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity; Belongs to the LecA/PllA lectin family. (122 aa)
pqsHProbable FAD-dependent monooxygenase; Involved in the terminal step of the biosynthesis of quinolone which in addition to serve as a potent signal for quorum sensing, chelates iron and promotes the formation of membrane vesicles (MVs). Catalyzes the hydroxylation of 2-heptyl-4-quinolone (C7-HHQ) to yield 2-heptyl-3-hydroxy-4-quinolone (PQS). Belongs to the 3-hydroxybenzoate 6-hydroxylase family. (382 aa)
qteEQuorum threshold expression element, QteE; Product name confidence: Class 1: Function experimentally demonstrated in P. aeruginosa. (190 aa)
lapProbable aminopeptidase; A secreted aminopeptidase. Acts on free N-terminal amino groups with a very strong preference for Leu- followed by Met- and Ala-, and no activity on Glu- or Gly- peptides (an exhaustive analysis was not performed). Both the AP56 and AP28 forms have activity. (536 aa)
apeBHypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (429 aa)
PA3327Probable non-ribosomal peptide synthetase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (2352 aa)
PA3328Probable FAD-dependent monooxygenase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (388 aa)
PA3329Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (442 aa)
PA3330Probable short chain dehydrogenase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (304 aa)
PA3331Cytochrome P450; Product name confidence: Class 2 (High similarity to functionally studied protein); Belongs to the cytochrome P450 family. (418 aa)
PA3332Conserved hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences); Belongs to the PhzA/PhzB family. (141 aa)
PA3334Probable acyl carrier protein; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (79 aa)
PA3335Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (250 aa)
PA3336Probable major facilitator superfamily (MFS) transporter; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (388 aa)
lecBFucose-binding lectin PA-IIL; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (115 aa)
rhlIAutoinducer synthesis protein RhlI; Required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR and thus acts in elastase biosynthesis regulation. (201 aa)
rhlRTranscriptional regulator RhlR; Necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). Binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone). (241 aa)
rhlBRhamnosyltransferase chain B; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (426 aa)
rhlARhamnosyltransferase chain A; Required for rhamnolipid surfactant production. Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta- hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. In vitro results establish that RhlA is highly selective for 10-carbon acyl-ACP intermediates [...] (295 aa)
lasBElastase LasB; Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase. Autocatalyses processing of its pro-peptide. Processes the pro-peptide of pro-chitin-binding protein (cbpD). Involved in the pathogenesis of P.aeruginosa infections. (498 aa)
rhlATP-dependent RNA helicase RhlB; DEAD-box RNA helicase involved in RNA degradation. Has RNA- dependent ATPase activity and unwinds double-stranded RNA. Belongs to the DEAD box helicase family. RhlB subfamily. (397 aa)
PA4078Probable nonribosomal peptide synthetase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (991 aa)
PA4079Probable dehydrogenase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (229 aa)
pivProtease IV; Lysine-specific endoprotease. Involved in corneal virulence; Belongs to the peptidase S1 family. (462 aa)
pqsLProbable FAD-dependent monooxygenase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (398 aa)
phzMProbable phenazine-specific methyltransferase; Involved in the biosynthesis of pyocyanine, a blue-pigmented phenazine derivative, which plays a role in virulence. Converts phenazine-1-carboxylate (PCA) to 5-methylphenazine-1-carboxylate (5- methyl-PCA); Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. (334 aa)
phzA1Probable phenazine biosynthesis protein; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule. PhzA1 (operon phzA1B1C1E1F1G1) has a role in the biosynthesis of the phenazine during planktonic growth. (162 aa)
phzB1Probable phenazine biosynthesis protein; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule. PhzB1 (operon phzA1B1C1E1F1G1) has a role in the biosynthesis of the phenazine during planktonic growth; Belongs to the PhzA/PhzB family. (162 aa)
phzC1Phenazine biosynthesis protein PhzC; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (405 aa)
phzD1Phenazine biosynthesis protein PhzD; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (207 aa)
phzF1Probable phenazine biosynthesis protein; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (278 aa)
phzSFlavin-containing monooxygenase; Involved in the biosynthesis of pyocyanine, a blue-pigmented phenazine derivative, which plays a role in virulence. Catalyzes the oxidative decarboxylation of 5-methylphenazine-1-carboxylate (5-methyl- PCA) to pyocyanine. Can also act on phenazine-1-carboxylate (PCA), converting it into 1-hydroxyphenazine (1-HP). However, PCA is a poor substrate. (402 aa)
PA4907Probable short-chain dehydrogenase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (253 aa)
PA5220Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (273 aa)
Your Current Organism:
Pseudomonas aeruginosa
NCBI taxonomy Id: 208964
Other names: P. aeruginosa PAO1, Pseudomonas aeruginosa PAO1, Pseudomonas sp. PAO1
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