Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Alginate biosynthesis protein Alg8; Processive enzyme that polymerizes GDP-mannuronic acid. Belongs to the glycosyltransferase 2 family.

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Phosphomannomutase AlgC; Highly reversible phosphoryltransferase. The phosphomannomutase activity produces a precursor for alginate polymerization, the alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics. Also involved in core lipopolysaccaride (LPS) biosynthesis due to its phosphoglucomutase activity. Essential for rhamnolipid production, an exoproduct correlated with pathogenicity. Required for biofilm production. The reaction proceeds via 2 processive phosphoryl transferase reactions; first from enzyme-phospho-Ser-1 [...]

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

GDP-mannose 6-dehydrogenase AlgD; Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics.

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Alginate production outer membrane protein AlgE precursor; Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Alginate-c5-mannuronan-epimerase AlgG; Catalyzes the epimerization of beta-D-mannuronate to alpha-L- guluronate during the synthesis of the linear polysaccharide alginate. In addition, is part of a periplasmic protein complex that protects alginate from degradation by AlgL by channeling the newly formed alginate polymer through a scaffold that transfers the alginate polymer through the periplasmic space to the outer membrane secretin AlgE.

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Alginate biosynthetic protein AlgK precursor; May be involved in the polymerization of mannuronate to alginate.

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Poly(beta-d-mannuronate) lyase precursor AlgL; Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. May serve to degrade mislocalized alginate that is trapped in the periplasmic space. Acts preferentially on non-acetylated alginate or its precursor mannuronan. Is able to catalyze cleavage adjacent to either mannuronate or guluronate residues in alginate. Exhaustive digestion of alginate by AlgL generates dimeric and trimeric products. In addition to its enzymatic function, AlgL appears [...]

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Alginate biosynthesis protein AlgX; Plays two roles in the biosynthesis of the exopolysaccharide alginate: protects alginate from degradation as the polymer traverses the periplasm, and also plays a role in its O-acetylation. Acetylation of alginate causes the cells in the biofilm to adhere better to lung epithelium, form microcolonies, and resist the effects of the host immune system and/or antibiotics. Displays a low acetylesterase activity in vitro using a pseudosubstrate, 3-carboxyumbelliferyl acetate. Probably has acetyltransferase activity in vivo. Belongs to the AlgX family.

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

Hypothetical protein; Involved in the final reduction of the elongation cycle of fatty acid synthesis (FAS II). Catalyzes the reduction of a carbon- carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). It can use both crotonyl-CoA and trans-2- decenoyl-ACP. It is able to convert trans-2-enoyl-ACP of different length (C2 to C16) to the corresponding acyl-ACP. Belongs to the TER reductase family.

Probable epimerase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene).

O-antigen translocase; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa).

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Alginate biosynthesis protein Alg8; Processive enzyme that polymerizes GDP-mannuronic acid. Belongs to the glycosyltransferase 2 family.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Two-component response regulator AlgB; Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. Positive regulator of the alginate biosynthetic gene AlgD.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Phosphomannomutase AlgC; Highly reversible phosphoryltransferase. The phosphomannomutase activity produces a precursor for alginate polymerization, the alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics. Also involved in core lipopolysaccaride (LPS) biosynthesis due to its phosphoglucomutase activity. Essential for rhamnolipid production, an exoproduct correlated with pathogenicity. Required for biofilm production. The reaction proceeds via 2 processive phosphoryl transferase reactions; first from enzyme-phospho-Ser-1 [...]

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

GDP-mannose 6-dehydrogenase AlgD; Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Alginate production outer membrane protein AlgE precursor; Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Alginate-c5-mannuronan-epimerase AlgG; Catalyzes the epimerization of beta-D-mannuronate to alpha-L- guluronate during the synthesis of the linear polysaccharide alginate. In addition, is part of a periplasmic protein complex that protects alginate from degradation by AlgL by channeling the newly formed alginate polymer through a scaffold that transfers the alginate polymer through the periplasmic space to the outer membrane secretin AlgE.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Alginate biosynthetic protein AlgK precursor; May be involved in the polymerization of mannuronate to alginate.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Poly(beta-d-mannuronate) lyase precursor AlgL; Catalyzes the depolymerization of alginate by cleaving the beta-1,4 glycosidic bond between two adjacent sugar residues via a beta-elimination mechanism. May serve to degrade mislocalized alginate that is trapped in the periplasmic space. Acts preferentially on non-acetylated alginate or its precursor mannuronan. Is able to catalyze cleavage adjacent to either mannuronate or guluronate residues in alginate. Exhaustive digestion of alginate by AlgL generates dimeric and trimeric products. In addition to its enzymatic function, AlgL appears [...]

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Alginate biosynthesis regulatory protein AlgR; Positive regulator of the algD gene, which codes for a GDP- mannose dehydrogenase, a key step enzyme in the alginate biosynthesis pathway.

Alginate biosynthesis protein Alg44; Required for alginate biosynthesis. Belongs to the Alg44 family.

Sigma factor AlgU; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis.