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phzA2 phzA2 phzD2 phzD2 phzF2 phzF2 PA2274 PA2274 pqsD pqsD exoS exoS rhlA rhlA rhlR rhlR nosR nosR lecB lecB gapA gapA pqsH pqsH lecA lecA piv piv pqsL pqsL mexG mexG opmD opmD phzM phzM phzA1 phzA1 phzS phzS pchR pchR sodB sodB sodM sodM catB catB pvdS pvdS fumC1 fumC1 rhl rhl pqsC pqsC pqsB pqsB pqsA pqsA fumC2 fumC2 hcpC hcpC phnA phnA phnB phnB pqsE pqsE mvfR mvfR lasR lasR pcrV pcrV pcrH pcrH popB popB chiC chiC popD popD exsC exsC exsE exsE exsB exsB pscE pscE qscR qscR
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query proteins and first shell of interactors
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second shell of interactors
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proteins of unknown 3D structure
filled nodes:
a 3D structure is known or predicted
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experimentally determined
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phzA2Probable phenazine biosynthesis protein; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule having important roles in virulence, competition and biological control. PhzA2 (operon phzA2B2C2E2F2G2) has a role in the biosynthesis of the phenazine during both planktonic growth and biofilm development, and in host infection during biofilm development. (162 aa)
phzD2Phenazine biosynthesis protein PhzD; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule. PhzD1 (operon phzA1B1C1E1F1G1) has a role in the biosynthesis of the phenazine during planktonic growth. Catalyzes the hydrolysis of the vinyl ether functional group of 2-amino-2-deoxyisochorismate (ADIC), yielding pyruvate and trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA). Also able to act on isochorismate, chorismate and 4-amino-4-deoxychorismate (ADC) as substrates ; Belongs to the isochorismatase family. (207 aa)
phzF2Probable phenazine biosynthesis protein; Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known. (278 aa)
PA2274Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). (125 aa)
pqsD3-oxoacyl-[acyl-carrier-protein] synthase III; Required for the biosynthesis of a number of signaling molecules, such as the quinolone signal 2-heptyl-3-hydroxy-4(1H)- quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ) and 2,4- dihydroxyquinoline (DHQ). These molecules are required for normal biofilm formation. Catalyzes the transfer of the anthraniloyl moiety from anthraniloyl-CoA to malonyl-CoA to form 2-aminobenzoylacetyl-CoA. The first step of the reaction is the formation of a covalent anthraniloyl-PqsD intermediate. Next, the short-lived intermediate 3-(2-aminophenyl)- 3-oxopropa [...] (337 aa)
exoSExoenzyme S; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (453 aa)
rhlARhamnosyltransferase chain A; Required for rhamnolipid surfactant production. Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta- hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. In vitro results establish that RhlA is highly selective for 10-carbon acyl-ACP intermediates [...] (295 aa)
rhlRTranscriptional regulator RhlR; Necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). Binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone). (241 aa)
nosRRegulatory protein NosR; Transcriptional activator of the nitrous-oxide reductase gene NosZ. (715 aa)
lecBFucose-binding lectin PA-IIL; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (115 aa)
gapAGlyceraldehyde 3-phosphate dehydrogenase; Catalyzes the oxidative phosphorylation of glyceraldehyde 3- phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG. (334 aa)
pqsHProbable FAD-dependent monooxygenase; Involved in the terminal step of the biosynthesis of quinolone which in addition to serve as a potent signal for quorum sensing, chelates iron and promotes the formation of membrane vesicles (MVs). Catalyzes the hydroxylation of 2-heptyl-4-quinolone (C7-HHQ) to yield 2-heptyl-3-hydroxy-4-quinolone (PQS). Belongs to the 3-hydroxybenzoate 6-hydroxylase family. (382 aa)
lecALecA; D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl- beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity; Belongs to the LecA/PllA lectin family. (122 aa)
pivProtease IV; Lysine-specific endoprotease. Involved in corneal virulence; Belongs to the peptidase S1 family. (462 aa)
pqsLProbable FAD-dependent monooxygenase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene). (398 aa)
mexGHypothetical protein; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (148 aa)
opmDProbable outer membrane protein precursor; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (487 aa)
phzMProbable phenazine-specific methyltransferase; Involved in the biosynthesis of pyocyanine, a blue-pigmented phenazine derivative, which plays a role in virulence. Converts phenazine-1-carboxylate (PCA) to 5-methylphenazine-1-carboxylate (5- methyl-PCA); Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. (334 aa)
phzA1Probable phenazine biosynthesis protein; Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule. PhzA1 (operon phzA1B1C1E1F1G1) has a role in the biosynthesis of the phenazine during planktonic growth. (162 aa)
phzSFlavin-containing monooxygenase; Involved in the biosynthesis of pyocyanine, a blue-pigmented phenazine derivative, which plays a role in virulence. Catalyzes the oxidative decarboxylation of 5-methylphenazine-1-carboxylate (5-methyl- PCA) to pyocyanine. Can also act on phenazine-1-carboxylate (PCA), converting it into 1-hydroxyphenazine (1-HP). However, PCA is a poor substrate. (402 aa)
pchRTranscriptional regulator PchR; Positive activator of the genes for pyochelin and ferripyochelin receptors. (296 aa)
sodBSuperoxide dismutase; Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems; Belongs to the iron/manganese superoxide dismutase family. (193 aa)
sodMSuperoxide dismutase; Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems; Belongs to the iron/manganese superoxide dismutase family. (203 aa)
catBMuconate cycloisomerase I; Product name confidence: Class 2 (High similarity to functionally studied protein); Belongs to the mandelate racemase/muconate lactonizing enzyme family. (373 aa)
pvdSSigma factor PvdS; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa); Belongs to the sigma-70 factor family. ECF subfamily. (187 aa)
fumC1Fumarate hydratase; Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate; Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. (458 aa)
rhlATP-dependent RNA helicase RhlB; DEAD-box RNA helicase involved in RNA degradation. Has RNA- dependent ATPase activity and unwinds double-stranded RNA. Belongs to the DEAD box helicase family. RhlB subfamily. (397 aa)
pqsCPqsC; Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)- quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. The PqsC/PqsB complex catalyzes the condensation of 2-aminobenzoylacetate (2-ABA) and octanoyl-CoA to form HHQ. First, PqsC acquires an octanoyl group from octanoyl-CoA and forms an octanoyl-PqsC intermediate. Then, together with PqsB, it catalyzes the coupling of 2-ABA with the octanoate group, leading to decarboxylation and dehydration, and re [...] (348 aa)
pqsBPqsB; Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)- quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. The PqsC/PqsB complex catalyzes the condensation of 2-aminobenzoylacetate (2-ABA) and octanoyl-CoA to form HHQ. PqsB, together with PqsC, catalyzes the coupling of 2-ABA with the octanoate group, leading to decarboxylation and dehydration, and resulting in closure of the quinoline ring. PqsB is probably required for the proper folding of PqsC ra [...] (283 aa)
pqsAProbable coenzyme A ligase; Catalyzes the formation of anthraniloyl-CoA, which is the priming step for entry into the Pseudomonas quinolone signal (PQS) biosynthetic pathway. Also active on a variety of aromatic substrates, including benzoate and chloro and fluoro derivatives of anthranilate. (517 aa)
fumC2Fumarate hydratase; Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate; Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. (464 aa)
hcpCSecreted protein Hcp; Product name confidence: Class 2 (High similarity to functionally studied protein). (172 aa)
phnAAnthranilate synthase component I; Part of a heterotetrameric complex that catalyzes the two- step biosynthesis of anthranilate, a precursor for Pseudomonas quinolone signal (2-heptyl-3-hydroxy-4-quinolone; PQS) production which is required to induce the genes for the biosynthesis of the virulence factor pyocyanine (PCN), a characteristic blue-green phenazine pigment produced by P.aeruginosa. In the first step, the glutamine-binding beta subunit (PhnB) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with [...] (530 aa)
phnBAnthranilate synthase component II; Part of a heterotetrameric complex that catalyzes the two- step biosynthesis of anthranilate, a precursor for Pseudomonas quinolone signal (2-heptyl-3-hydroxy-4-quinolone; PQS) production which is required to induce the genes for the biosynthesis of the virulence factor pyocyanine (PCN), a characteristic blue-green phenazine pigment produced by P.aeruginosa. In the first step, the glutamine-binding beta subunit (PhnB) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with [...] (200 aa)
pqsEQuinolone signal response protein; Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)- quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. Catalyzes the hydrolysis of the intermediate 2-aminobenzoylacetyl-CoA (2-ABA-CoA) to form 2- aminobenzoylacetate (2-ABA), the precursor of HHQ. In vitro, can also hydrolyze other substrates such as S-ethyl-acetothioacetate and acetoacetyl-CoA, but is inactive against anthraniloyl-CoA, malonyl-CoA and octanoyl-CoA. Be [...] (301 aa)
mvfRTranscriptional regulator MvfR; Transcription regulator that plays a critical role in virulence by positively regulating the expression of multiple quorum sensing (QS)-regulated virulence factors, genes involved in protein secretion, translation, response to oxidative stress and the phnAB operon. At the stationary phase, negatively autoregulates its function through cleavage and translocation to the extracellular space ; Belongs to the LysR transcriptional regulatory family. (332 aa)
lasRTranscriptional regulator LasR; Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer; Belongs to the autoinducer-regulated transcriptional regulatory protein family. (239 aa)
pcrVType III secretion protein PcrV; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (294 aa)
pcrHRegulatory protein PcrH; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (167 aa)
popBTranslocator protein PopB; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (390 aa)
chiCChitinase; Product name confidence: Class 2 (High similarity to functionally studied protein); Belongs to the glycosyl hydrolase 18 family. (483 aa)
popDTranslocator outer membrane protein PopD precursor; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (295 aa)
exsCExsC, exoenzyme S synthesis protein C precursor; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (145 aa)
exsEExsE; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (81 aa)
exsBExoenzyme S synthesis protein B; Involved in the synthesis of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. Pilot protein that is required for the proper localization of the secretin PscC in the outer membrane. Necessary for full in vivo virulence ; Belongs to the ExsB/YscW family. (137 aa)
pscEType III export protein PscE; Prevents premature polymerization of PscF, along with PscG, within the cytoplasm. Required for type III secretion needle assembly. Also required for cytotoxicity by influencing PscF levels. Belongs to the YscE family. (67 aa)
qscRQuorum-sensing control repressor; Product name confidence: Class 1 (Function experimentally demonstrated in P. aeruginosa). (237 aa)
Your Current Organism:
Pseudomonas aeruginosa
NCBI taxonomy Id: 208964
Other names: P. aeruginosa PAO1, Pseudomonas aeruginosa PAO1, Pseudomonas sp. PAO1
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