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| | STY3653 | Dihydroxyacid dehydratase; Similar to Escherichia coli dihydroxy-acid dehydratase SW:ILVD_ECOLI () (605 aa) fasta scores: E(): 0, 93.9% id in 602 aa and to Haemophilus influenzae dihydroxy-acid dehydratase IlvD SW:ILVD_HAEIN (P44851) (612 aa) fasta scores: E(): 0, 76.9% id in 614 aa. Note that the N-terminus of this CDS is more similar to the H. influenzae IlvD; Fasta hit to YAGF_ECOLI (655 aa), 30% identity in 543 aa overlap; Orthologue of E. coli ilvD (ILVD_ECOLI); Fasta hit to ILVD_ECOLI (605 aa), 94% identity in 602 aa overlap; Belongs to the IlvD/Edd family. (616 aa) |
| | STY0001 | Thr operon leader peptide; This protein is involved in control of the biosynthesis of threonine. (21 aa) |
| | STY0002 | Aspartokinase I/homoserine dehydrogenase I; Orthologue of E. coli thrA (AK1H_ECOLI); Fasta hit to AK1H_ECOLI (820 aa), 94% identity in 820 aa overlap; In the C-terminal section; belongs to the homoserine dehydrogenase family. (820 aa) |
| | STY0003 | Homoserine kinase; Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate; Belongs to the GHMP kinase family. Homoserine kinase subfamily. (309 aa) |
| | STY0004 | Threonine synthase; Orthologue of E. coli thrC (THRC_ECOLI); Fasta hit to THRC_ECOLI (428 aa), 93% identity in 428 aa overlap. (428 aa) |
| | STY0055 | isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (944 aa) |
| | STY0073 | Dihydrodipicolinate reductase; Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate; Belongs to the DapB family. (273 aa) |
| | STY0076 | Carbamoyl-phosphate synthase small chain; Orthologue of E. coli carA (CARA_ECOLI); Fasta hit to CARA_ECOLI (382 aa), 93% identity in 382 aa overlap; Belongs to the CarA family. (382 aa) |
| | STY0077 | Carbamoyl-phosphate synthase large chain; Orthologue of E. coli carB (CARB_ECOLI); Fasta hit to CARB_ECOLI (1072 aa), 98% identity in 1072 aa overlap. (1075 aa) |
| | STY0102 | Dihydrofolate reductase type I; Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. (159 aa) |
| | STY0129 | 3-isopropylmalate dehydratase; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. (201 aa) |
| | STY0130 | 3-isopropylmalate dehydratase; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (466 aa) |
| | STY0131 | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. (363 aa) |
| | STY0132 | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. (523 aa) |
| | STY0133 | Leu operon leader peptide; Involved in control of the biosynthesis of leucine. (28 aa) |
| | ilvI | Similar to Escherichia coli acetolactate synthase isozyme iii large subunit ILVI SW:ILVI_ECOLI (P00893; P78045) fasta scores: E(): 0, 92.2% id in 574 aa, and to Buchnera aphidicola acetolactate synthase large subunit ILVI SW:ILVI_BUCAP (O85293) fasta scores: E(): 0, 70.5% id in 572 aa. (574 aa) |
| | STY0136 | Acetolactate synthase isozyme III small subunit; Orthologue of E. coli ilvH (ILVH_ECOLI); Fasta hit to ILVH_ECOLI (163 aa), 96% identity in 163 aa overlap. (164 aa) |
| | STY0198 | Aspartate 1-decarboxylase; Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine. (126 aa) |
| | STY0210 | glutamyl-tRNA synthetase-related protein; Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon; Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily. (298 aa) |
| | mtn | MTA/SAH nucleosidase; Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'- methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation. Belongs to the PNP/UDP phosphorylas [...] (232 aa) |
| | STY0236 | 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase; Orthologue of E. coli dapD (DAPD_ECOLI); Fasta hit to DAPD_ECOLI (274 aa), 96% identity in 274 aa overlap; Belongs to the transferase hexapeptide repeat family. (274 aa) |
| | STY0259 | Lysine decarboxylase; Fasta hit to ADI_ECOLI (755 aa), 35% identity in 720 aa overlap; Fasta hit to DCOS_ECOLI (732 aa), 32% identity in 694 aa overlap; Fasta hit to DCOR_ECOLI (731 aa), 35% identity in 626 aa overlap; Fasta hit to DCLY_ECOLI (715 aa), 71% identity in 710 aa overlap; Orthologue of E. coli ldcC (DCLZ_ECOLI); Fasta hit to DCLZ_ECOLI (713 aa), 91% identity in 712 aa overlap. (713 aa) |
| | STY0269 | prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (572 aa) |
| | STY0356 | Conserved hypothetical protein; Orthologue of E. coli yafJ (YAFJ_ECOLI); Fasta hit to YAFJ_ECOLI (255 aa), 93% identity in 255 aa overlap. (255 aa) |
| | STY0366 | Glutamate 5-kinase; Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate. (367 aa) |
| | STY0367 | Gamma-glutamyl phosphate reductase; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. Belongs to the gamma-glutamyl phosphate reductase family. (416 aa) |
| | STY0419 | Pyrroline-5-carboxylate reductase; Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. (269 aa) |
| | STY0421 | Shikimate kinase II; Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate. (181 aa) |
| | arcC | Carbamate kinase; Similar to Escherichia coli carbamate kinase arcC SW:ARCC_ECOLI (P37306; P77419) (297 aa) fasta scores: E(): 0, 86.9% id in 297 aa, and to Pseudomonas aeruginosa carbamate kinase arcC SW:ARCC_PSEAE (P13982) (310 aa) fasta scores: E(): 0, 52.7% id in 296 aa; Fasta hit to ARCM_ECOLI (316 aa), 40% identity in 317 aa overlap; Fasta hit to ARCL_ECOLI (310 aa), 40% identity in 315 aa overlap; Orthologue of E. coli ybcF (ARCC_ECOLI); Fasta hit to ARCC_ECOLI (297 aa), 87% identity in 297 aa overlap. (297 aa) |
| | STY0585 | cysteinyl-tRNA synthetase; Orthologue of E. coli cysS (SYC_ECOLI); Fasta hit to SYC_ECOLI (461 aa), 94% identity in 461 aa overlap; Belongs to the class-I aminoacyl-tRNA synthetase family. (461 aa) |
| | STY0588 | FolD bifunctional protein [includes: methylenetetrahydrofolate dehydrogenase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. (288 aa) |
| | cobD | Putative aminotransferase CobD; Decarboxylates L-threonine-O-3-phosphate to yield (R)-1- amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin. (364 aa) |
| | STY0699 | leucyl-tRNA synthetase; Orthologue of E. coli leuS (SYL_ECOLI); Fasta hit to SYL_ECOLI (860 aa), 95% identity in 860 aa overlap; Belongs to the class-I aminoacyl-tRNA synthetase family. (860 aa) |
| | STY0718 | Asparagine synthetase B; Orthologue of E. coli asnB (ASNB_ECOLI); Fasta hit to ASNB_ECOLI (553 aa), 95% identity in 553 aa overlap. (554 aa) |
| | STY0724 | glutaminyl-tRNA synthetase; Orthologue of E. coli glnS (SYQ_ECOLI); Fasta hit to SYQ_ECOLI (553 aa), 96% identity in 554 aa overlap. (555 aa) |
| | STY0780 | Dihydrolipoamide succinyltransferase component (E2); E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2- oxoglutarate to succinyl-CoA and CO(2). (402 aa) |
| | STY0801 | Phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthetase) phenylalanine repressible; Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino- heptulosonate-7-phosphate (DAHP). (350 aa) |
| | hutI | Imidazolonepropionase; Similar to Yersinia pestis imidazolonepropionase hutI SW:HUTI_YERPE (Q9ZC74) (406 aa) fasta scores: E(): 0, 50.0% id in 400 aa, and to Rhizobium meliloti imidazolonepropionase hutI SW:HUTI_RHIME (O31196) (369 aa) fasta scores: E(): 0, 51.8% id in 340 aa; Belongs to the metallo-dependent hydrolases superfamily. HutI family. (407 aa) |
| | hutG | Formiminoglutamase; Catalyzes the conversion of N-formimidoyl-L-glutamate to L- glutamate and formamide; Belongs to the arginase family. (313 aa) |
| | hutC | Similar to Klebsiella aerogenes histidine utilization repressor hutC SW:HUTC_KLEAE (P12380) (241 aa) fasta scores: E(): 0, 78.8% id in 241 aa, and to Pseudomonas putida histidine utilization repressor hutC SW:HUTC_PSEPU (P22773) (248 aa) fasta scores: E(): 0, 63.5% id in 230 aa. (241 aa) |
| | hutU | Urocanate hydratase; Catalyzes the conversion of urocanate to 4-imidazolone-5- propionate. (561 aa) |
| | hutH | Similar to Pseudomonas putida histidine ammonia-lyase SW:HUTH_PSEPU (P21310) (509 aa) fasta scores: E(): 0, 77.4% id in 500 aa, and to Rhizobium meliloti histidine ammonia-lyase SW:HUTH_RHIME () (511 aa) fasta scores: E(): 0, 52.6% id in 498 aa; Belongs to the PAL/histidase family. (506 aa) |
| | ybjU | Similar to Escherichia coli L-allo-threonine aldolase ybjU SW:LTAA_ECOLI (P75823) (333 aa) fasta scores: E(): 0, 89.2% id in 333 aa, and to Aeromonas jandaei L-allo-threonine aldolase ltaA SW:LTAA_AERJA (O07051) (338 aa) fasta scores: E(): 0, 53.5% id in 333 aa; Orthologue of E. coli LTAA_ECOLI; Fasta hit to LTAA_ECOLI (333 aa), 89% identity in 333 aa overlap. (333 aa) |
| | STY0961 | seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (430 aa) |
| | STY0977 | Phosphoserine aminotransferase; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. (362 aa) |
| | STY0978 | 3-phosphoshikimate 1-carboxyvinyltransferase; Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. (427 aa) |
| | STY1000 | Aspartate aminotransferase; Fasta hit to TYRB_ECOLI (397 aa), 43% identity in 397 aa overlap; Orthologue of E. coli aspC (AAT_ECOLI); Fasta hit to AAT_ECOLI (396 aa), 96% identity in 396 aa overlap; Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. (396 aa) |
| | asnS | Similar to Escherichia coli asparaginyl-tRNA synthetase SW:SYN_ECOLI (P17242) fasta scores: E(): 0, 94.6% id in 465 aa. (466 aa) |
| | STY1159 | Proline dehydrogenase (proline oxidase); Oxidizes proline to glutamate for use as a carbon and nitrogen source; Belongs to the aldehyde dehydrogenase family. In the N-terminal section; belongs to the proline dehydrogenase family. (1320 aa) |
| | STY1324 | Tryptophan synthase alpha chain; The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. Belongs to the TrpA family. (268 aa) |
| | STY1325 | Tryptophan synthase beta chain; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. (397 aa) |
| | STY1326 | Indole-3-glycerol phosphate synthase; Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the TrpF domain; the second reaction is catalyzed by the synthase, coded by the TrpC domain (By similarity). (452 aa) |
| | STY1327 | Anthranilate synthase component II; Catalyzes the transfer of the phosphoribosyl group of 5- phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'- phosphoribosyl)-anthranilate (PRA). (531 aa) |
| | STY1328 | Anthranilate synthase component I; Orthologue of E. coli trpE (TRPE_ECOLI); Fasta hit to TRPE_ECOLI (520 aa), 90% identity in 520 aa overlap. (520 aa) |
| | STY1382 | Putative mandelate racemase / muconate lactonizing enzyme family protein; Similar to Pseudomonas sp chloromuconate cycloisomerase TcbD SW:TCBD_PSESQ (P27099) (370 aa) fasta scores: E(): 3.3e-10, 26.5% id in 336 aa; Orthologue of E. coli YCJG_ECOLI; Fasta hit to YCJG_ECOLI (321 aa), 85% identity in 321 aa overlap. (321 aa) |
| | STY1467 | Putative aldehyde dehydrogenase; Catalyzes the oxidation 4-aminobutanal (gamma- aminobutyraldehyde) to 4-aminobutanoate (gamma-aminobutyrate or GABA). This is the second step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate via 4- aminobutanal. Also functions as a 5-aminopentanal dehydrogenase in a a L-lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. (481 aa) |
| | STY1556 | Starvation sensing protein RspA; Orthologue of E. coli rspA (RSPA_ECOLI); Fasta hit to RSPA_ECOLI (404 aa), 95% identity in 404 aa overlap. (404 aa) |
| | STY1673 | tyrosyl-tRNA synthetase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (424 aa) |
| | STY1741 | Orf 245 protein; Similar to Salmonella typhimurium Orf 245 protein orf 245 TR:Q9Z4S4 (EMBL:AJ224978) (245 aa) fasta scores: E(): 0, 99.6% id in 245 aa; putative pathogenicity island protein. (245 aa) |
| | STY1750 | Putative amintransferase; Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L- selenocysteine. Selenocysteine lyase activity is however unsure in vivo. (406 aa) |
| | aroD | 3-dehydroquinate dehydratase; Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis- dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate. The reaction involves the formation of an imine intermediate between the keto group of 3-dehydroquinate and the epsylon-amino group of Lys-170 at the active site; Belongs to the type-I 3-dehydroquinase family. (252 aa) |
| | STY1763 | 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase; Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino- heptulosonate-7-phosphate (DAHP). (348 aa) |
| | STY1772 | phenylalanyl-tRNA synthetase beta chain; Orthologue of E. coli pheT (SYFB_ECOLI); Fasta hit to SYFB_ECOLI (795 aa), 92% identity in 795 aa overlap; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (795 aa) |
| | STY1773 | phenylalanyl-tRNA synthetase alpha chain; Orthologue of E. coli pheS (SYFA_ECOLI); Fasta hit to SYFA_ECOLI (327 aa), 98% identity in 327 aa overlap; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (327 aa) |
| | STY1778 | threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (642 aa) |
| | astE | Succinylglutamate desuccinylase; Transforms N(2)-succinylglutamate into succinate and glutamate; Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily. (322 aa) |
| | astB | Succinylarginine dihydrolase; Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)- succinylornithine, ammonia and CO(2). (447 aa) |
| | astD | Succinylglutamic semialdehyde dehydrogenase; Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate. (428 aa) |
| | astA | Arginine N-succinyltransferase; Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine. (344 aa) |
| | astC | Succinylornithine transaminase; Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. AstC subfamily. (408 aa) |
| | STY1815 | NADP-specific glutamate dehydrogenase; Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. (447 aa) |
| | STY1817 | Selenophosphate synthase; Synthesizes selenophosphate from selenide and ATP. (347 aa) |
| | STY1820 | Orthologue of E. coli ansA (ASG1_ECOLI); Fasta hit to ASG1_ECOLI (338 aa), 94% identity in 338 aa overlap. (338 aa) |
| | gdhA | Similar to Thermotoga maritima glutamate dehydrogenase gdha or gdh or tm1015 SW:DHE3_THEMA (P96110) (415 aa) fasta scores: E(): 0, 55.9% id in 388 aa, and to Clostridium difficile NAD-specific glutamate dehydrogenase gluD SW:DHE2_CLODI (P27346) (421 aa) fasta scores: E(): 0, 47.8% id in 391 aa; Paralogue of E. coli gdhA (DHE4_ECOLI); Fasta hit to DHE4_ECOLI (447 aa), 34% identity in 363 aa overlap; Belongs to the Glu/Leu/Phe/Val dehydrogenases family. (389 aa) |
| | STY1930 | Alanine racemase; Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA. (356 aa) |
| | STY1931 | D-amino acid dehydrogenase; Oxidative deamination of D-amino acids. (432 aa) |
| | STY2109 | aspartyl-tRNA synthetase; Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (590 aa) |
| | STY2117 | arginyl-tRNA synthetase; Orthologue of E. coli argS (SYR_ECOLI); Fasta hit to SYR_ECOLI (577 aa), 95% identity in 577 aa overlap. (577 aa) |
| | STY2161 | Putative deaminase; Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine; Belongs to the ACC deaminase/D-cysteine desulfhydrase family. (328 aa) |
| | cbiP | Putative cobyric acid synthase; Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. (506 aa) |
| | cbiA | Cobyrinic acid A,C-diamide synthase; Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source; Belongs to the CobB/CbiA family. (474 aa) |
| | hisL | His operon leader peptide; Similar to Escherichia coli, and Salmonella typhimurium his operon leader peptide hisL SW:LPHI_ECOLI (P03058) (16 aa) fasta scores: E(): 0.00021, 81.3% id in 16 aa, and to Klebsiella pneumoniae hiso leader peptide TR:Q48439 (EMBL:K01997) (16 aa) fasta scores: E(): 0.0079, 68.8% id in 16 aa. (13 aa) |
| | STY2280 | ATP phosphoribosyltransferase; Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity. (299 aa) |
| | STY2281 | Histidinol dehydrogenase; Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine. (434 aa) |
| | STY2282 | Similar to Salmonella typhimurium histidinol-phosphate aminotransferase hisC SW:HIS8_SALTY (P10369) (359 aa) fasta scores: E(): 0, 98.6% id in 359 aa; Orthologue of E. coli hisC (HIS8_ECOLI); Fasta hit to HIS8_ECOLI (356 aa), 89% identity in 355 aa overlap; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily. (359 aa) |
| | STY2283 | Imidazoleglycerol-phosphate dehydratase; Similar to Salmonella typhimurium histidine biosynthesis bifunctional protein HisB [includes: histidinol-phosphatase hisB SW:HIS7_SALTY (P10368) (354 aa) fasta scores: E(): 0, 97.5% id in 355 aa; Orthologue of E. coli hisB (HIS7_ECOLI); Fasta hit to HIS7_ECOLI (355 aa), 96% identity in 355 aa overlap; In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family. (355 aa) |
| | STY2284 | Amidotransferase; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF (By similarity). (196 aa) |
| | STY2285 | Similar to Salmonella typhimurium phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase hisA SW:HIS4_SALTY (P10372) (246 aa) fasta scores: E(): 0, 96.3% id in 246 aa; Orthologue of E. coli hisA (HIS4_ECOLI); Fasta hit to HIS4_ECOLI (245 aa), 94% identity in 244 aa overlap. (245 aa) |
| | STY2286 | Cyclase HisF; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit (By similarity). (258 aa) |
| | STY2287 | phosphoribosyl-AMP cyclohydrolase / phosphoribosyl-ATP pyrophosphohydrolase; Similar to Salmonella typhimurium histidine biosynthesis bifunctional protein HisI [includes: phosphoribosyl-AMP cyclohydrolase hisI SW:HIS2_SALTY (P10367) (203 aa) fasta scores: E(): 0, 97.5% id in 203 aa; Orthologue of E. coli hisI (HIS2_ECOLI); Fasta hit to HIS2_ECOLI (203 aa), 91% identity in 203 aa overlap; In the C-terminal section; belongs to the PRA-PH family. (203 aa) |
| | STY2327 | Similar to acetyltransferases e.g. Staphylococcus xylosus serine acetyltransferase cysE SW:CYSE_STAXY (P77985) (216 aa) fasta scores: E(): 8.2e-12, 39.7% id in 141 aa and to part of Arabidopsis thaliana serine acetyltransferase TR:Q43297 (EMBL:Z34888) (314 aa) fasta scores: E(): 9.4e-12, 39.3% id in 140 aa; Orthologue of E. coli wcaB (WCAB_ECOLI); Fasta hit to WCAB_ECOLI (162 aa), 91% identity in 160 aa overlap; Belongs to the transferase hexapeptide repeat family. (162 aa) |
| | metG | methionyl-tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (677 aa) |
| | STY2406 | glutathione-S-transferase-family protein; Similar to e.g. Pseudomonas sp. U2 glutathione-S-transferase homolog from plasmid pWWU2 nagL TR:O86043 (EMBL:AF081362) (212 aa) fasta scores: E(): 2.4e-28, 42.1% id in 214 aa and Homo sapiens maleylacetoacetate isomerase gstz1 or maaI SW:MAAI_HUMAN (O43708; O15308; O75430) (216 aa) fasta scores: E(): 1.7e-24, 40.8% id in 211 aa. May be maleylpyruvate isomerase, given the presence of a putative gentisate 1,2-dioxygenase upstream. (214 aa) |
| | STY2431 | Putative hydrolase; Similar, except at N-terminus, to phosphoserine phosphatases e.g. Escherichia coli phosphoserine phosphatase serB SW:SERB_ECOLI (P06862) (322 aa) fasta scores: E(): 4.2e-22, 41.0% id in 188 aa. (295 aa) |
| | STY2521 | Putative MR-MLE-family protein; Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy- L-rhamnonate (KDR). (405 aa) |
| | STY2592 | Amidophosphoribosyltransferase; Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine; In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. (505 aa) |
| | STY2600 | Putative semialdehyde dehydrogenase; Orthologue of E. coli usg (USG_ECOLI); Fasta hit to USG_ECOLI (337 aa), 89% identity in 337 aa overlap; Belongs to the aspartate-semialdehyde dehydrogenase family. (337 aa) |
| | STY2616 | Chorismate synthase; Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system. (361 aa) |
| | STY2654 | glutamyl-tRNA synthetase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). (474 aa) |
| | STY2665 | Putative sulfate transport protein CysZ; High affinity, high specificity proton-dependent sulfate transporter, which mediates sulfate uptake. Provides the sulfur source for the cysteine synthesis pathway; Belongs to the CysZ family. (253 aa) |
| | STY2666 | Cysteine synthase A; Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product (By similarity). (323 aa) |
| | STY2677 | Cysteine synthase B; Similar to Salmonella typhimurium LT2 cysteine synthase B cysM SW:CYSM_SALTY (P29848) (303 aa) fasta scores: E(): 0, 98.7% id in 303 aa; Fasta hit to CYSK_ECOLI (322 aa), 44% identity in 307 aa overlap; Orthologue of E. coli cysM (CYSM_ECOLI); Fasta hit to CYSM_ECOLI (303 aa), 93% identity in 303 aa overlap; Belongs to the cysteine synthase/cystathionine beta- synthase family. (303 aa) |
| | eutC | Similar to Salmonella typhimurium ethanolamine ammonia-lyase light chain eutC SW:EUTC_SALTY (P19265) (298 aa) fasta scores: E(): 0, 99.3% id in 298 aa; Orthologue of E. coli eutC (EUTC_ECOLI); Fasta hit to EUTC_ECOLI (295 aa), 91% identity in 298 aa overlap; Belongs to the EutC family. (298 aa) |
| | eutB | Similar to Salmonella typhimurium ethanolamine ammonia-lyase heavy chain SW:EUTB_SALTY (P19264) (453 aa) fasta scores: E(): 0, 99.8% id in 453 aa; Orthologue of E. coli eutB (EUTB_ECOLI); Fasta hit to EUTB_ECOLI (453 aa), 98% identity in 453 aa overlap. (453 aa) |
| | STY2721 | Succinyl-diaminopimelate desuccinylase; Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls; Belongs to the peptidase M20A family. DapE subfamily. (375 aa) |
| | STY2727 | Dihydrodipicolinate synthase; Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). (292 aa) |
| | STY2751 | GMP synthase (glutamine-hydrolyzing); Catalyzes the synthesis of GMP from XMP. (525 aa) |
| | STY2767 | Similar to Salmonella typhimurium histidyl-tRNA synthetase SW:SYH_SALTY (O52765) (423 aa) fasta scores: E(): 0, 99.3% id in 423 aa; Orthologue of E. coli hisS (SYH_ECOLI); Fasta hit to SYH_ECOLI (423 aa), 95% identity in 423 aa overlap. (424 aa) |
| | STY2802 | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (417 aa) |
| | STY2806 | Similar to Salmonella typhimurium lysine decarboxylase, inducible SW:DCLY_SALTY (Q60002) (715 aa) fasta scores: E(): 0, 97.5% id in 715 aa; Fasta hit to DCOS_ECOLI (732 aa), 33% identity in 623 aa overlap; Fasta hit to ADI_ECOLI (755 aa), 36% identity in 740 aa overlap; Fasta hit to DCLZ_ECOLI (713 aa), 70% identity in 710 aa overlap; Fasta hit to DCOR_ECOLI (731 aa), 34% identity in 616 aa overlap; Orthologue of E. coli cadA (DCLY_ECOLI); Fasta hit to DCLY_ECOLI (715 aa), 92% identity in 714 aa overlap; Belongs to the Orn/Lys/Arg decarboxylase class-I family. (714 aa) |
| | STY2812 | Phosphoribosylformylglycineamide synthetase; Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. (1295 aa) |
| | STY2854 | Chorismate mutase-P/prephenate dehydratase; Orthologue of E. coli pheA (PHEA_ECOLI); Fasta hit to PHEA_ECOLI (386 aa), 91% identity in 385 aa overlap. (386 aa) |
| | STY2856 | Chorismate mutase/prephenate dehydrogenase; Orthologue of E. coli tyrA (TYRA_ECOLI); Fasta hit to TYRA_ECOLI (373 aa), 95% identity in 372 aa overlap. (373 aa) |
| | STY2857 | Phospho-2-dehydro-3-deoxyheptonate aldolase, tyr-sensitive; Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino- heptulosonate-7-phosphate (DAHP); Belongs to the class-I DAHP synthase family. (356 aa) |
| | STY2909 | Gab protein homolog; Acts as an alpha-ketoglutarate-dependent dioxygenase catalyzing hydroxylation of glutarate (GA) to L-2-hydroxyglutarate (L2HG). Functions in a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. (328 aa) |
| | STY2910 | Putative GAB DTP gene cluster repressor; Catalyzes the dehydrogenation of L-2-hydroxyglutarate (L2HG) to alpha-ketoglutarate and couples to the respiratory chain by feeding electrons from the reaction into the membrane quinone pool. Functions in a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. Reaction=(S)-2-hydroxyglutarate + a quinone = 2-oxoglutarate + a quinol; Xref=Rhea:RHEA:58664, ChEBI:CHEBI:16782, ChEBI:CHEBI:16810, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:58665; Belongs to the L2 [...] (422 aa) |
| | STY2911 | Succinate-semialdehyde dehydrogenase; Fasta hit to ALDA_ECOLI (478 aa), 39% identity in 464 aa overlap; Fasta hit to FEAB_ECOLI (499 aa), 36% identity in 480 aa overlap; Fasta hit to YNEI_ECOLI (462 aa), 32% identity in 445 aa overlap; Fasta hit to ALDB_ECOLI (512 aa), 33% identity in 490 aa overlap; Fasta hit to DHAB_ECOLI (489 aa), 37% identity in 481 aa overlap; Fasta hit to DHAL_ECOLI (495 aa), 34% identity in 485 aa overlap; Fasta hit to ASTD_ECOLI (492 aa), 32% identity in 470 aa overlap; Fasta hit to YDCW_ECOLI (474 aa), 37% identity in 477 aa overlap; Orthologue of E. coli gabD [...] (482 aa) |
| | STY2912 | 4-aminobutyrate aminotransferase; Fasta hit to GSA_ECOLI (426 aa), 32% identity in 421 aa overlap; Fasta hit to ARGM_ECOLI (406 aa), 34% identity in 404 aa overlap; Fasta hit to ARGD_ECOLI (405 aa), 36% identity in 411 aa overlap; Fasta hit to BIOA_ECOLI (429 aa), 32% identity in 353 aa overlap; Fasta hit to GOAG_ECOLI (421 aa), 53% identity in 421 aa overlap; Orthologue of E. coli gabT (GABT_ECOLI); Fasta hit to GABT_ECOLI (426 aa), 89% identity in 426 aa overlap; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. (427 aa) |
| | STY2948 | alanyl-tRNA synthetase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain; Belongs to the class-II aminoacyl-tRNA synthetase family. (876 aa) |
| | STY3075 | Sulfite reductase (NADPH) hemoprotein alpha subunit; Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. (570 aa) |
| | STY3076 | Sulfite reductase (NADPH) flavoprotein beta subunit; Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component; Belongs to the NADPH-dependent sulphite reductase flavoprotein subunit CysJ family. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. (599 aa) |
| | STY3082 | CTP synthetase; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates. (545 aa) |
| | ygcY | Similar to Escherichia coli probable glucarate dehydratase 2 ygcY SW:GUD2_ECOLI (Q46915) (446 aa) fasta scores: E(): 0, 95.1% id in 446 aa; Fasta hit to GUD1_ECOLI (446 aa), 65% identity in 448 aa overlap; Orthologue of E. coli GUD2_ECOLI; Fasta hit to GUD2_ECOLI (446 aa), 95% identity in 446 aa overlap. (446 aa) |
| | STY3124 | Similar to many NifS-like aminotransferases e.g. Synechocystis sp NifS TR:Q55793 (EMBL:D64004) (420 aa) fasta scores: E(): 0, 40.4% id in 403 aa; Fasta hit to P77444 (406 aa), 44% identity in 404 aa overlap; Orthologue of E. coli Q46925; Fasta hit to Q46925 (401 aa), 89% identity in 401 aa overlap. (401 aa) |
| | STY3130 | N-acetylglutamate synthase; Orthologue of E. coli argA (ARGA_ECOLI); Fasta hit to ARGA_ECOLI (443 aa), 94% identity in 443 aa overlap; Belongs to the acetyltransferase family. ArgA subfamily. (443 aa) |
| | STY3157 | Diaminopimelate decarboxylase; Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine. (420 aa) |
| | STY3196 | Lysyl tRNA synthetase (LysRS); Fasta hit to SYK2_ECOLI (504 aa), 90% identity in 504 aa overlap; Orthologue of E. coli lysS (SYK1_ECOLI); Fasta hit to SYK1_ECOLI (504 aa), 95% identity in 504 aa overlap; Belongs to the class-II aminoacyl-tRNA synthetase family. (505 aa) |
| | STY3209 | Glycine dehydrogenase (decarboxylating); The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein; Belongs to the GcvP family. (957 aa) |
| | STY3210 | Glycine cleavage system H protein; The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein. (130 aa) |
| | STY3211 | Aminomethyltransferase; The glycine cleavage system catalyzes the degradation of glycine. (364 aa) |
| | STY3218 | D-3-phosphoglycerate dehydrogenase; Orthologue of E. coli serA (SERA_ECOLI); Fasta hit to SERA_ECOLI (409 aa), 96% identity in 409 aa overlap; Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. (410 aa) |
| | STY3238 | Agmatine ureohydrolase; Catalyzes the formation of putrescine from agmatine. Belongs to the arginase family. Agmatinase subfamily. (306 aa) |
| | speA | Biosynthetic arginine decarboxylase; Catalyzes the biosynthesis of agmatine from arginine. (658 aa) |
| | STY3259 | L-asparaginase; Highly similar to Escherichia coli L-asparaginase II precursor AnsB SW:ASG2_ECOLI (P00805) (348 aa) fasta scores: E(): 0, 92.8% id in 348 aa. Contains a possible N-terminal signal sequence; Belongs to the asparaginase 1 family. (348 aa) |
| | STY3333 | Beta-cystathionase; Fasta hit to METB_ECOLI (386 aa), 32% identity in 398 aa overlap; Orthologue of E. coli metC (METC_ECOLI); Fasta hit to METC_ECOLI (395 aa), 87% identity in 395 aa overlap. (395 aa) |
| | STY3396 | Probable aminotransferase; Catalyzes the aminotransferase reaction from putrescine to 2- oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. This is the first step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate (gamma-aminobutyrate or GABA) via 4- aminobutanal. Also functions as a cadaverine transaminase in a a L- lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. (429 aa) |
| | tdcD | Propionate kinase; Catalyzes the conversion of propionyl phosphate and ADP to propionate and ATP. (402 aa) |
| | STY3427 | Catabolic threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. (329 aa) |
| | STY3452 | Conserved hypothetical protein; Similar to several including: Bacillus subtilis general stress protein 18 yfkM SW:GS18_BACSU (P80876) (171 aa) fasta scores: E(): 0, 64.9% id in 168 aa, and to Pyrococcus furiosus protease I pfpI SW:PFPI_PYRFU (Q51732) (166 aa) fasta scores: E(): 6.5e-24, 47.9% id in 167 aa; Orthologue of E. coli yhbO (YHBO_ECOLI); Fasta hit to YHBO_ECOLI (172 aa), 95% identity in 172 aa overlap. (172 aa) |
| | STY3470 | Argininosuccinate synthetase; Orthologue of E. coli argG (ASSY_ECOLI); Fasta hit to ASSY_ECOLI (446 aa), 96% identity in 446 aa overlap; Belongs to the argininosuccinate synthase family. Type 2 subfamily. (469 aa) |
| | STY3510 | Similar to Escherichia coli glutamate synthase [NADPH] large chain precursor GltB SW:GLTB_ECOLI (P09831) (1517 aa) fasta scores: E(): 0, 95.4% id in 1486 aa and to Pseudomonas aeruginosa glutamate synthase large subunit GltB TR:P95456 (EMBL:U81261) (1482 aa) fasta scores: E(): 0, 60.9% id in 1476 aa. (1486 aa) |
| | argR | Arginine repressor; Regulates arginine biosynthesis genes. (156 aa) |
| | STY3594 | 5-methyltetrahydropteroyltriglutamate- homocysteine methyltransferase; Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. (754 aa) |
| | STY3595 | Trans-activator of metE and metH; Control of the last step in methionine biosynthesis; MetR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression (By similarity). Belongs to the LysR transcriptional regulatory family. (317 aa) |
| | STY3612 | Diaminopimelate epimerase; Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan. (274 aa) |
| | STY3648 | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. (491 aa) |
| | STY3649 | LysR-family regulatory protein for ilvC expression; This protein activates the transcription of the IlvC gene in the presence of acetolactate or acetohydroxybutyrate. IlvY is also a negative regulator of its own expression (By similarity). Belongs to the LysR transcriptional regulatory family. (295 aa) |
| | STY3652 | Threonine deaminase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. (514 aa) |
| | STY3654 | Branched-chain amino-acid aminotransferase; Acts on leucine, isoleucine and valine. (309 aa) |
| | STY3656 | Acetolactate synthase large subunit; Fasta hit to ILVB_ECOLI (562 aa), 47% identity in 554 aa overlap; Fasta hit to ILVI_ECOLI (574 aa), 43% identity in 564 aa overlap; Orthologue of E. coli ilvG_2 (ILVG_ECOLI); Fasta hit to ILVG_ECOLI (548 aa), 92% identity in 548 aa overlap. (548 aa) |
| | ilvL | ilvGMEDA operon attenuator peptide; Similar to Escherichia coli, Serratia marcescens, Salmonella typhimurium, and ilvgmeda operon leader peptide IlvL SW:LPID_ECOLI (P03060) (32 aa) fasta scores: E(): 7.4e-12, 100.0% id in 32 aa. (32 aa) |
| | STY3750 | Argininosuccinate lyase; Orthologue of E. coli argH (ARLY_ECOLI); Fasta hit to ARLY_ECOLI (457 aa), 95% identity in 456 aa overlap. (458 aa) |
| | STY3751 | Acetylglutamate kinase; Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate. (257 aa) |
| | STY3752 | N-acetyl-gamma-glutamyl-phosphate reductase; Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. (334 aa) |
| | STY3753 | Acetylornithine deacetylase; Displays a broad specificity and can also deacylate substrates such as acetylarginine, acetylhistidine or acetylglutamate semialdehyde. (383 aa) |
| | STY3761 | 5,10 methylenetetrahydrofolate reductase; Orthologue of E. coli metF (METF_ECOLI); Fasta hit to METF_ECOLI (296 aa), 96% identity in 296 aa overlap; Belongs to the methylenetetrahydrofolate reductase family. (296 aa) |
| | STY3763 | Alanine racemase; Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids; Belongs to the alanine racemase family. (368 aa) |
| | STY3764 | Putative serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (419 aa) |
| | STY3768 | Bifunctional aspartokinase II/homoserine dehydrogenase IIcan I write; Fasta hit to AK1H_ECOLI (820 aa), 30% identity in 823 aa overlap; Orthologue of E. coli metL (AK2H_ECOLI); Fasta hit to AK2H_ECOLI (809 aa), 94% identity in 809 aa overlap; In the C-terminal section; belongs to the homoserine dehydrogenase family. (810 aa) |
| | STY3769 | Cystathionine gamma-synthase; Fasta hit to METC_ECOLI (395 aa), 31% identity in 394 aa overlap; Orthologue of E. coli metB (METB_ECOLI); Fasta hit to METB_ECOLI (386 aa), 96% identity in 386 aa overlap. (386 aa) |
| | STY3770 | Repressor of the methionine regulon; This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis. It is also autoregulated (By similarity); Belongs to the MetJ family. (105 aa) |
| | STY3850 | Conserved hypothetical protein; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. Belongs to the DTD family. (145 aa) |
| | STY3874 | Glutamine synthetase; Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia; Belongs to the glutamine synthetase family. (469 aa) |
| | STY3901 | Asparagine synthetase A; Orthologue of E. coli asnA (ASNA_ECOLI); Fasta hit to ASNA_ECOLI (330 aa), 95% identity in 330 aa overlap; Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. (330 aa) |
| | STY3917 | Glucosamine--fructose-6-phosphate aminotransferase; Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source. (609 aa) |
| | STY3977 | D-serine dehydratase; Orthologue of E. coli dsdA (SDHD_ECOLI); Fasta hit to SDHD_ECOLI (442 aa), 89% identity in 442 aa overlap. (440 aa) |
| | STY3986 | Acetohydroxy acid synthase I, small subunit; Fasta hit to ILVG_ECOLI (548 aa), 46% identity in 550 aa overlap; Fasta hit to GCL_ECOLI (592 aa), 35% identity in 507 aa overlap; Fasta hit to ILVI_ECOLI (574 aa), 40% identity in 566 aa overlap; Orthologue of E. coli ilvB (ILVB_ECOLI); Fasta hit to ILVB_ECOLI (562 aa), 92% identity in 562 aa overlap. (562 aa) |
| | STY3987 | Acetohydroxy acid synthase I, small subunit; Orthologue of E. coli ilvN (ILVN_ECOLI); Fasta hit to ILVN_ECOLI (96 aa), 90% identity in 96 aa overlap. (96 aa) |
| | STY4086 | 2-amino-3-ketobutyrate coenzyme A ligase; Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA. (398 aa) |
| | tdh | Threonine 3-dehydrogenase; Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2- amino-3-ketobutyrate; Belongs to the zinc-containing alcohol dehydrogenase family. (341 aa) |
| | cysE | Similar to Escherichia coli serine acetyltransferase cysE SW:CYSE_ECOLI (P05796) (273 aa) fasta scores: E(): 0, 95.6% id in 273 aa; Belongs to the transferase hexapeptide repeat family. (273 aa) |
| | STY4099 | Putative racemase; Similar to Streptomyces coelicolor putative racemase SCE65.16C TR:Q9RKF7 (EMBL:AL133236) (361 aa) fasta scores: E(): 0, 34.0% id in 356 aa, and to Pseudomonas putida mandelate racemase mdlA SW:MANR_PSEPU (P11444) (359 aa) fasta scores: E(): 1.9e-25, 29.5% id in 353 aa; Belongs to the mandelate racemase/muconate lactonizing enzyme family. (398 aa) |
| | STY4143 | glycine-tRNA synthetase, alpha subunit; Orthologue of E. coli glyQ (SYGA_ECOLI); Fasta hit to SYGA_ECOLI (303 aa), 99% identity in 303 aa overlap. (303 aa) |
| | STY4144 | glycine-tRNA synthetase, beta subunit; Orthologue of E. coli glyS (SYGB_ECOLI); Fasta hit to SYGB_ECOLI (688 aa), 94% identity in 688 aa overlap. (689 aa) |
| | STY4263 | Hypothetical protein; Similar to Salmonella typhimurium ORF 245 protein TR:Q9Z4S4 (EMBL:AJ224978) (245 aa) fasta scores: E(): 0, 64.1% id in 245 aa. (253 aa) |
| | STY4271 | Aspartate-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. (368 aa) |
| | aroK | Shikimate kinase I; Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate; Belongs to the shikimate kinase family. (173 aa) |
| | aroB | 3-dehydroquinate synthase; Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ); Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family. (362 aa) |
| | trpS | tryptophanyl-tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (334 aa) |
| | pabA | Similar to Salmonella typhimurium para-aminobenzoate synthase glutamine amidotransferase component ii pabA SW:PABA_SALTY (P06193) (187 aa) fasta scores: E(): 0, 99.5% id in 187 aa, and to Escherichia coli para-aminobenzoate synthase glutamine amidotransferase component ii pabA SW:PABA_ECOLI (P00903) (187 aa) fasta scores: E(): 0, 85.0% id in 187 aa. (187 aa) |
| | argD | Acetylornithine aminotransferase; Involved in both the arginine and lysine biosynthetic pathways. (405 aa) |
| | aroE | Shikimate dehydrogenase; Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA). (272 aa) |
| | metA | Homoserine O-succinyltransferase; Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. (309 aa) |
| | STY4405 | B12-dependent homocysteine-N5-methyltetrahydrofolate transmethylase; Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. (1227 aa) |
| | STY4416 | Similar to Escherichia coli lysine-sensitive aspartokinase III LysC SW:AK3_ECOLI (P08660) (449 aa) fasta scores: E(): 0, 93.3% id in 449 aa; Belongs to the aspartokinase family. (449 aa) |
| | alr | Alanine racemase; Catalyzes the interconversion of L-alanine and D-alanine. Provides the D-alanine required for cell wall biosynthesis (By similarity). (359 aa) |
| | STY4444 | Similar to Salmonella typhimurium aromatic-amino-acid aminotransferase SW:TYRB_SALTY (P74861) (397 aa) fasta scores: E(): 0, 98.0% id in 397 aa, and to Escherichia coli aromatic-amino-acid aminotransferase tyrB SW:TYRB_ECOLI (P04693) (397 aa) fasta scores: E(): 0, 88.9% id in 397 aa. (397 aa) |
| | adi | Arginine decarboxylase; Similar to Escherichia coli biodegradative arginine decarboxylase SW:ADI_ECOLI (P28629) (755 aa) fasta scores: E(): 0, 93.1% id in 755 aa, and to Escherichia coli lysine decarboxylase, constitutive SW:DCLZ_ECOLI (P52095) (713 aa) fasta scores: E(): 0, 36.2% id in 737 aa. (756 aa) |
| | aspA | Similar to Escherichia coli Aspartate ammonia-lyase aspA SW:ASPA_ECOLI (P04422) (478 aa) fasta scores: E(): 0, 97.1% id in 478 aa, and to Serratia marcescens Aspartate ammonia-lyase aspA SW:ASPA_SERMA (P33109) (478 aa) fasta scores: E(): 0, 89.7% id in 478 aa. (478 aa) |
| | STY4704 | lysyl-tRNA synthetase; With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta- lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF- P 'Lys-34'; Belongs to the class-II aminoacyl-tRNA synthetase family. EpmA subfamily. (325 aa) |
| | pyrB | Similar to Salmonella typhimurium aspartate carbamoyltransferase catalytic chain PyrB SW:PYRB_SALTY (P08420) (309 aa) fasta scores: E(): 0, 98.4% id in 310 aa, and to Escherichia coli aspartate carbamoyltransferase catalytic chain PyrB SW:PYRB_ECOLI (P00479) (310 aa) fasta scores: E(): 0, 95.2% id in 310 aa; Fasta hit to OTC2_ECOLI (333 aa), 32% identity in 298 aa overlap; Fasta hit to OTC1_ECOLI (333 aa), 32% identity in 295 aa overlap. (311 aa) |
| | STY4801 | Putative transcriptional regulator; Regulates arginine biosynthesis genes. (162 aa) |
| | STY4803 | Ornithine carbamoyltransferase; Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. (334 aa) |
| | STY4804 | Carbamate kinase; Similar to Pseudomonas aeruginosa carbamate kinase SW:ARCC_PSEAE (P13982) (310 aa) fasta scores: E(): 0, 54.6% id in 306 aa, and to Synechocystis sp carbamate kinase SW:ARCC_SYNY3 (P74733) (308 aa) fasta scores: E(): 0, 53.5% id in 301 aa. (310 aa) |
| | STY4805 | Arginine deiminase; Similar to Pseudomonas aeruginosa arginine deiminase SW:ARCA_PSEAE (P13981) (417 aa) fasta scores: E(): 0, 46.8% id in 408 aa, and to Rhizobium etli arginine deiminase TR:O31017 (EMBL:AF025543) (409 aa) fasta scores: E(): 0, 47.3% id in 402 aa. (406 aa) |
| | STY4807 | Ornithine carbamoyltransferase; Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. (334 aa) |
| | valS | valyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (951 aa) |
| | trpS2 | Similar to Clostridium longisporum tryptophanyl-tRNA synthetase TrpS or TrsA SW:SYW_CLOLO (Q46127) (341 aa) fasta scores: E(): 0, 54.8% id in 330 aa, and to Thermotoga maritima tryptophanyl-tRNA synthetase TrpS SW:SYW_THEMA (Q9WYW2) (328 aa) fasta scores: E(): 0, 36.5% id in 329 aa. Note that the E.coli trpS ortholog is STY4315; Belongs to the class-I aminoacyl-tRNA synthetase family. (337 aa) |
| | serB | Similar to Escherichia coli phosphoserine phosphatase SerB SW:SERB_ECOLI (P06862) (322 aa) fasta scores: E(): 0, 93.5% id in 322 aa, and to Haemophilus influenzae phosphoserine phosphatase SerB SW:SERB_HAEIN (P44997) (314 aa) fasta scores: E(): 0, 51.5% id in 303 aa. (322 aa) |
