STRINGSTRING
STY0443 STY0443 STY4902 STY4902 trpS2 trpS2 valS valS miaE miaE miaA miaA STY4704 STY4704 STY4439 STY4439 fmt fmt trpS trpS STY4144 STY4144 STY4143 STY4143 STY4113 STY4113 STY4101 STY4101 STY4060 STY4060 trmH trmH STY3939 STY3939 STY3850 STY3850 STY3745 STY3745 STY3564 STY3564 STY3465 STY3465 STY3383 STY3383 STY3264 STY3264 STY3196 STY3196 yqcB yqcB STY3053 STY3053 STY2948 STY2948 STY2861 STY2861 STY2843 STY2843 STY2835 STY2835 STY2770 STY2770 STY2767 STY2767 STY2654 STY2654 STY2610 STY2610 STY2599 STY2599 STY2404 STY2404 metG metG STY2117 STY2117 STY2109 STY2109 STY1909 STY1909 STY1902 STY1902 STY1835 STY1835 STY1778 STY1778 STY1773 STY1773 STY1772 STY1772 STY1673 STY1673 STY1399 STY1399 asnS asnS smtA smtA STY0961 STY0961 STY0724 STY0724 STY0699 STY0699 STY0585 STY0585 STY0561 STY0561 STY0541 STY0541 STY0464 STY0464 STY0442 STY0442 STY0269 STY0269 STY0265 STY0265 STY0210 STY0210 rluA rluA STY0055 STY0055
Nodes:
Network nodes represent proteins
splice isoforms or post-translational modifications are collapsed, i.e. each node represents all the proteins produced by a single, protein-coding gene locus.
Node Color
colored nodes:
query proteins and first shell of interactors
white nodes:
second shell of interactors
Node Content
empty nodes:
proteins of unknown 3D structure
filled nodes:
a 3D structure is known or predicted
Edges:
Edges represent protein-protein associations
associations are meant to be specific and meaningful, i.e. proteins jointly contribute to a shared function; this does not necessarily mean they are physically binding to each other.
Known Interactions
from curated databases
experimentally determined
Predicted Interactions
gene neighborhood
gene fusions
gene co-occurrence
Others
textmining
co-expression
protein homology
Your Input:
STY0443Queuine tRNA-ribosyltransferase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form th [...] (375 aa)
STY4902Similar to Pseudomonas aeruginosa conserved hypothetical protein PA1357 TR:AAG04746 (EMBL:AE004565) (158 aa) fasta scores: E(): 0, 69.7% id in 152 aa, and to Bacillus subtilis hypothetical 17.5 kDa protein YwhH TR:P71000 (EMBL:Z80360) (157 aa) fasta scores: E(): 2e-27, 48.7% id in 154 aa. (152 aa)
trpS2Similar to Clostridium longisporum tryptophanyl-tRNA synthetase TrpS or TrsA SW:SYW_CLOLO (Q46127) (341 aa) fasta scores: E(): 0, 54.8% id in 330 aa, and to Thermotoga maritima tryptophanyl-tRNA synthetase TrpS SW:SYW_THEMA (Q9WYW2) (328 aa) fasta scores: E(): 0, 36.5% id in 329 aa. Note that the E.coli trpS ortholog is STY4315; Belongs to the class-I aminoacyl-tRNA synthetase family. (337 aa)
valSvalyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (951 aa)
miaEtRNA hydroxylase; Similar to Salmonella typhimurium tRNA-hydroxylase MiaE SW:MIAE_SALTY (Q08015) (270 aa) fasta scores: E(): 0, 98.9% id in 270 aa, and to Vibrio cholerae tRNA-hydroxylase VC1910 TR:Q9KQT8 (EMBL:AE004266) (258 aa) fasta scores: E(): 0, 60.7% id in 247 aa. Start is set at equivalent of codon 17 in S. typhimurium gene, as upstream start id out of frame. This start is closer to taht of the V. cholerae gene, and has a good RBS. (254 aa)
miaAtRNA delta-2-isopentenylpyrophosphate (IPP) transferase; Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A); Belongs to the IPP transferase family. (316 aa)
STY4704lysyl-tRNA synthetase; With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta- lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF- P 'Lys-34'; Belongs to the class-II aminoacyl-tRNA synthetase family. EpmA subfamily. (325 aa)
STY4439Conserved hypothetical protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. (332 aa)
fmtmethionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus. (315 aa)
trpStryptophanyl-tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (334 aa)
STY4144glycine-tRNA synthetase, beta subunit; Orthologue of E. coli glyS (SYGB_ECOLI); Fasta hit to SYGB_ECOLI (688 aa), 94% identity in 688 aa overlap. (689 aa)
STY4143glycine-tRNA synthetase, alpha subunit; Orthologue of E. coli glyQ (SYGA_ECOLI); Fasta hit to SYGA_ECOLI (303 aa), 99% identity in 303 aa overlap. (303 aa)
STY4113L-seryl-tRNA(Ser) selenium transferase; Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis; Belongs to the SelA family. (463 aa)
STY4101Putative RNA-methyltransferase; Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. (157 aa)
STY4060RNase PH; Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. (238 aa)
trmHtRNA (guanosine-2'-O)-methyltransferase; Catalyzes the 2'-O methylation of guanosine at position 18 in tRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (229 aa)
STY3939RNase P, protein component; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (119 aa)
STY3850Conserved hypothetical protein; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. Belongs to the DTD family. (145 aa)
STY3745tRNA (uracil-5)-methyltransferase; Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA). (366 aa)
STY3564Conserved hypothetical protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines; Belongs to the Dus family. DusB subfamily. (281 aa)
STY3465tRNA pseudouridine 55 synthase (psi55 synthase) (p35 protein); Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (314 aa)
STY3383tRNA nucleotidyltransferase; Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases. (413 aa)
STY3264Conserved hypothetical protein; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. (239 aa)
STY3196Lysyl tRNA synthetase (LysRS); Fasta hit to SYK2_ECOLI (504 aa), 90% identity in 504 aa overlap; Orthologue of E. coli lysS (SYK1_ECOLI); Fasta hit to SYK1_ECOLI (504 aa), 95% identity in 504 aa overlap; Belongs to the class-II aminoacyl-tRNA synthetase family. (505 aa)
yqcBPutative RNA pseudouridylate synthase; Responsible for synthesis of pseudouridine from uracil-65 in transfer RNAs; Belongs to the pseudouridine synthase RluA family. (260 aa)
STY3053Conserved hypothetical protein; Responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs; Belongs to the pseudouridine synthase TruD family. (349 aa)
STY2948alanyl-tRNA synthetase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain; Belongs to the class-II aminoacyl-tRNA synthetase family. (876 aa)
STY2861tRNA(guanine-N1)methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (255 aa)
STY2843Conserved hypothetical protein; Orthologue of E. coli YFIP_ECOLI; Fasta hit to YFIP_ECOLI (232 aa), 88% identity in 226 aa overlap. (226 aa)
STY2835Conserved hypothetical protein; Specifically methylates the adenine in position 37 of tRNA(1)(Val) (anticodon cmo5UAC). (245 aa)
STY2770Conserved hypothetical protein; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity; Belongs to the radical SAM superfamily. RlmN family. (388 aa)
STY2767Similar to Salmonella typhimurium histidyl-tRNA synthetase SW:SYH_SALTY (O52765) (423 aa) fasta scores: E(): 0, 99.3% id in 423 aa; Orthologue of E. coli hisS (SYH_ECOLI); Fasta hit to SYH_ECOLI (423 aa), 95% identity in 423 aa overlap. (424 aa)
STY2654glutamyl-tRNA synthetase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). (474 aa)
STY2610Conserved hypothetical protein; Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34; In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family. (686 aa)
STY2599tRNA pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (270 aa)
STY2404Conserved hypothetical protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs. Belongs to the Dus family. DusC subfamily. (312 aa)
metGmethionyl-tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (677 aa)
STY2117arginyl-tRNA synthetase; Orthologue of E. coli argS (SYR_ECOLI); Fasta hit to SYR_ECOLI (577 aa), 95% identity in 577 aa overlap. (577 aa)
STY2109aspartyl-tRNA synthetase; Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (590 aa)
STY1909peptidyl-tRNA hydrolase; The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death. (202 aa)
STY1902glutamyl-tRNA reductase; Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). (418 aa)
STY1835Conserved hypothetical protein; Orthologue of E. coli YEAK_ECOLI; Fasta hit to YEAK_ECOLI (167 aa), 87% identity in 165 aa overlap. (175 aa)
STY1778threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (642 aa)
STY1773phenylalanyl-tRNA synthetase alpha chain; Orthologue of E. coli pheS (SYFA_ECOLI); Fasta hit to SYFA_ECOLI (327 aa), 98% identity in 327 aa overlap; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (327 aa)
STY1772phenylalanyl-tRNA synthetase beta chain; Orthologue of E. coli pheT (SYFB_ECOLI); Fasta hit to SYFB_ECOLI (795 aa), 92% identity in 795 aa overlap; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (795 aa)
STY1673tyrosyl-tRNA synthetase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (424 aa)
STY1399Conserved hypothetical protein; Similar to Sulfolobus solfataricus hypothetical 25.3 kDa protein orf-c09_009 TR:Q9UX48 (EMBL:Y18930) (224 aa) fasta scores: E(): 2.1e-06, 28.7% id in 167 aa. (228 aa)
asnSSimilar to Escherichia coli asparaginyl-tRNA synthetase SW:SYN_ECOLI (P17242) fasta scores: E(): 0, 94.6% id in 465 aa. (466 aa)
smtASmtA protein; Catalyzes the methylation of 5-carboxymethoxyuridine (cmo5U) to form 5-methoxycarbonylmethoxyuridine (mcmo5U) at position 34 in tRNAs; Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoM family. (267 aa)
STY0961seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (430 aa)
STY0724glutaminyl-tRNA synthetase; Orthologue of E. coli glnS (SYQ_ECOLI); Fasta hit to SYQ_ECOLI (553 aa), 96% identity in 554 aa overlap. (555 aa)
STY0699leucyl-tRNA synthetase; Orthologue of E. coli leuS (SYL_ECOLI); Fasta hit to SYL_ECOLI (860 aa), 95% identity in 860 aa overlap; Belongs to the class-I aminoacyl-tRNA synthetase family. (860 aa)
STY0585cysteinyl-tRNA synthetase; Orthologue of E. coli cysS (SYC_ECOLI); Fasta hit to SYC_ECOLI (461 aa), 94% identity in 461 aa overlap; Belongs to the class-I aminoacyl-tRNA synthetase family. (461 aa)
STY0561Hypothetical ATP-binding protein; Involved in the post-transcriptional modification of the uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Catalyzes the conversion of 2-thiouridine (S2U-RNA) to 2- selenouridine (Se2U-RNA). Acts in a two-step process involving geranylation of 2-thiouridine (S2U) to S-geranyl-2-thiouridine (geS2U) and subsequent selenation of the latter derivative to 2-selenouridine (Se2U) in the tRNA chain. (364 aa)
STY0541Conserved hypothetical protein; Orthologue of E. coli YBAK_ECOLI; Fasta hit to YBAK_ECOLI (159 aa), 91% identity in 159 aa overlap; Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily. (159 aa)
STY0464Thiamine biosynthesis protein ThiI; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. (482 aa)
STY0442S-adenosylmethionine:tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). (354 aa)
STY0269prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (572 aa)
STY0265Putative release factor; Similar to Escherichia coli hypothetical 15.6 kDa protein in mesj-cutf intergenic region yaeJ SW:YAEJ_ECOLI (P40711) (140 aa) fasta scores: E(): 0, 84.6% id in 136 aa, and to Pseudomonas putida hypothetical 15.2 kDa protein in pcaj 3'region SW:YAEJ_PSEPU (P45388) (137 aa) fasta scores: E(): 9.5e-27, 57.0% id in 135 aa and to Salmonella typhimurium peptide chain release factor 2 prfB SW:RF2_SALTY (P28353) (365 aa) fasta scores: E(): 0.45, 29.6% id in 108 aa; Orthologue of E. coli yaeJ (YAEJ_ECOLI); Fasta hit to YAEJ_ECOLI (140 aa), 85% identity in 136 aa overlap. (140 aa)
STY0210glutamyl-tRNA synthetase-related protein; Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon; Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily. (298 aa)
rluARibosomal large subunit pseudouridine synthase A; Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs. (219 aa)
STY0055isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (944 aa)
Your Current Organism:
Salmonella enterica Typhi
NCBI taxonomy Id: 220341
Other names: S. enterica subsp. enterica serovar Typhi str. CT18, Salmonella enterica subsp. enterica serovar Typhi CT18, Salmonella enterica subsp. enterica serovar Typhi str. CT18, Salmonella enterica subsp. enterica serovar Typhi strain CT18, Salmonella typhi CT18
Server load: low (14%) [HD]
STRING is a Core Data Resource as designated by Global Biodata Coalition and ELIXIR.