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| | fliS | Flagellar assembly protein FliS; Essential for filament assembly. May act as a facilitator of flagellin (hag) secretion. Antagonizes translational repressor CsrA indirectly. Belongs to the FliS family. (133 aa) |
| | recG | Branch migrating ATP-dependent DNA helicase involved in DNA recombination and repair; Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y- DNA) (By similarity); Belongs to the helicase family. RecG subfamily. (682 aa) |
| | smc | Chromosome condensation and segregation SMC ATPase; Required for chromosome condensation and partitioning. Belongs to the SMC family. (1186 aa) |
| | ylqC | Putative RNA binding protein; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pf: putative factor; Belongs to the UPF0109 family. (81 aa) |
| | topA | DNA topoisomerase I; Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supe [...] (691 aa) |
| | flgB | Flagellar component of cell-proximal portion of basal-body rod; Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body. (129 aa) |
| | flgC | Flagellar component of cell-proximal portion of basal-body rod; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type s: structure; Belongs to the flagella basal body rod proteins family. (150 aa) |
| | fliE | Flagellar basal body protein; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type s: structure. (106 aa) |
| | fliF | Flagellar basal-body M-ring protein; The M ring may be actively involved in energy transduction. (536 aa) |
| | fliG | Flagellar motor switching and energizing component; One of the proteins that forms a switch complex that is proposed to be located at the base of the basal body. This complex interacts with chemotaxis proteins (such as CheY) in addition to contacting components of the motor that determine the direction of flagellar rotation; Belongs to the FliG family. (338 aa) |
| | fliH | Flagellar export apparatus component; Needed for flagellar regrowth and assembly. (208 aa) |
| | fliI | Flagellar-specific ATPase; Probable catalytic subunit of a protein translocase for flagellum-specific export, or a proton translocase involved in local circuits at the flagellum. (438 aa) |
| | fliJ | Flagellar synthesis chaperone; Flagellar protein that affects chemotactic events. (147 aa) |
| | ylxF | Putative kinesin-like protein; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type ps: putative structure. (204 aa) |
| | fliK | Flagellar hook-length control protein; Controls the length of the flagellar hook. (487 aa) |
| | flgD | Flagellar hook assembly protein; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type pf: putative factor; Belongs to the FlgD family. (140 aa) |
| | flgE | Flagellar hook protein; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type f: factor; Belongs to the flagella basal body rod proteins family. (264 aa) |
| | fliM | Flagellar motor switching and energizing component; One of the proteins that forms a switch complex that is proposed to be located at the base of the basal body. This complex interacts with chemotaxis proteins (such as CheY) in addition to contacting components of the motor that determine the direction of flagellar rotation; Belongs to the FliM family. (332 aa) |
| | fliY | Flagellar motor switching and energizing phosphatase; Component of the flagellar switch. Binds CheY-P and increases its hydrolysis rate in vitro. May function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P whereas CheC may function after addition of an attractant to cope with increased levels of CheY-P; Belongs to the FliN/MopA/SpaO family. (378 aa) |
| | fliZ | Flagellar regulatory protein; May be a structural component of the flagellum that anchors the rod to the membrane. (219 aa) |
| | fliP | Component of the flagellar export machinery; Plays a role in the flagellum-specific transport system. Belongs to the FliP/MopC/SpaP family. (221 aa) |
| | fliQ | Component of the flagellar export machinery; Role in flagellar biosynthesis; Belongs to the FliQ/MopD/SpaQ family. (89 aa) |
| | fliR | Component of the flagellar export machinery; Role in flagellar biosynthesis; Belongs to the FliR/MopE/SpaR family. (259 aa) |
| | flhB | Component of the flagellar export machinery; May be involved in the export of flagellum proteins; Belongs to the type III secretion exporter family. (360 aa) |
| | flhA | Component of the flagellar export machinery; Involved in the export of flagellum proteins. (677 aa) |
| | flhF | GTPase involved in the export of flagella; Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. (366 aa) |
| | ylxH | Essential component of the flagellar assembly machinery; Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF. (298 aa) |
| | frr | Ribosome recycling factor; Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another; Belongs to the RRF family. (185 aa) |
| | rimP | Ribosome maturation factor; Required for maturation of 30S ribosomal subunits. Belongs to the RimP family. (156 aa) |
| | spoVS | Stage V sporulation protein S; Induced early in sporulation under the control of sigma-H. Interferes with sporulation at an early stage. Seems to play a positive role in allowing cells to progress beyond stage V of sporulation. (86 aa) |
| | pksJ | Polyketide synthase of type I; Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. (5043 aa) |
| | pksL | Polyketide synthase of type I; Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. (4538 aa) |
| | pksM | Polyketide synthase; Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. (4262 aa) |
| | pksN | Polyketide synthase of type I; Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. (5488 aa) |
| | pksR | Polyketide synthase; Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. (2543 aa) |
| | cwlC | N-acetylmuramoyl-L-alanine amidase; Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze type A cell walls such as B.subtilis. Its main function is to lyze the mother cell wall peptidoglycan, playing a role during sporulation. Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family. (255 aa) |
| | yneA | Cell division inhibitor; Inhibits cell division during the SOS response. Affects a later stage of the cell division protein assembly, after the assembly of the Z ring, by probably suppressing recruitment of FtsL and/or DivIC to the division machinery (By similarity). (105 aa) |
| | ccdA | Cytochrome c-type biogenesis protein CcdA; Required for cytochrome c synthesis and stage V of sporulation. Might transfer reducing equivalents across the cytoplasmic membrane, promoting efficient disulfide bond isomerization of proteins localized on the outer surface of the membrane or in the spore coat. (235 aa) |
| | parE | Subunit B of DNA topoisomerase IV; Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule; Belongs to the type II topoisomerase family. ParE type 2 subfamily. (655 aa) |
| | parC | Subunit A of DNA topoisomerase IV; Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule; Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily. (806 aa) |
| | dacC | D-alanyl-D-alanine carboxypeptidase; Catalyzes DD-carboxypeptidase and transpeptidation reactions. (491 aa) |
| | ftsR | Transcriptional regulator (LysR family); Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase. (285 aa) |
| | rtbI | Ribonuclease toxin of toxin-antitoxin systems RttI-RttJ; Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with RecS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks; Belongs to the helicase family. RecQ subfamily. (591 aa) |
| | cwlS | Peptidoglycan hydrolase (cell wall-binding d,l-endopeptidase); Probably functions as a cell separation enzyme in addition to LytE and LytF. (414 aa) |
| | sspC | Small acid-soluble spore protein (alpha/beta-type SASP); SASP are bound to spore DNA. They are double-stranded DNA- binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light. (72 aa) |
| | yorI | Putative replicative DNA helicase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme. (504 aa) |
| | yonN | Putative HU-related DNA-binding protein; Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions; Belongs to the bacterial histone-like protein family. (92 aa) |
| | blyA | Bacteriophage SPbeta N-acetylmuramoyl-L-alanine amidase; Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Involved in prophage SP-beta-mediated cell lysis. (367 aa) |
| | dynA | Dynamin-like GTPase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme. (1193 aa) |
| | ypvA | Putative ATP-dependent helicase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative enzyme. (641 aa) |
| | yprA | Putative ATP-dependent helicase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme; Belongs to the helicase family. (749 aa) |
| | ponA | Peptidoglycan glycosyltransferase (penicillin-binding proteins 1A and 1B); Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross- linking of the peptide subunits). (914 aa) |
| | dinG | Damage inducible ATP-dependent 3'->5' nuclease; 3'-5' exonuclease. (931 aa) |
| | hbs | Non-specific DNA-binding protein HBsu; Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. Binds evenly across chromosome, does not display a preference for AT content. (92 aa) |
| | spoIVA | Morphogenetic stage IV sporulation protein; ATPase. Has a role at an early stage in the morphogenesis of the spore coat outer layers. Its ATP hydrolysis is required for proper assembly of the spore coat. Forms a basement layer around the outside surface of the forespore and self-assembles irreversibly into higher order structures by binding and hydrolyzing ATP thus creating a durable and stable platform upon which thereafter morphogenesis of the coat can take place. Required for proper localization of spore coat protein CotE and sporulation-specific proteins including SpoVM. (492 aa) |
| | engA | GTPase essential for ribosome 50S subunit assembly; GTPase that plays an essential role in the late steps of ribosome biogenesis; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family. (436 aa) |
| | sleB | Spore cortex-lytic enzyme; Could be a lytic transglycosylase. Required for spore cortex hydrolysis during germination. Interacts strongly but noncovalently with spore components. (305 aa) |
| | recQ | ATP-dependent DNA helicase; Probable DNA helicase. Required in synaptic and/or post- synaptic stages of recombination. Probably has overlapping function with RecQ (AC O34748). It probably acts to help generate ss-DNA from ds-DNA breaks. (496 aa) |
| | resC | Factor required for cytochrome c synthesis; Required for the biogenesis of c-type cytochromes. (391 aa) |
| | resB | Factor required for cytochrome c synthesis; Required for the biogenesis of c-type cytochromes. (542 aa) |
| | resA | Extracytoplasmic thioredoxin involved in cytochrome c maturation (lipoprotein); Thiol-disulfide oxidoreductase which is required in disulfide reduction during c-type cytochrome synthesis. May accept reducing equivalents from CcdA, leading to breakage of disulfide bonds in apocytochrome c; following this reduction heme can be covalently attached. Does not play a role in sporulation. Belongs to the thioredoxin family. ResA subfamily. (179 aa) |
| | dacB | D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5*); Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex). (382 aa) |
| | dacF | D-alanyl-D-alanine carboxypeptidase (penicilin binding protein); Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. (389 aa) |
| | oxaAB | Sec-independent factor for membrane protein insertion (YidC/SpoIIIJ family); Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins (By similarity). Also involved in protein secretion processes. It has an overlapping, although partly distinct, function compared to SpoIIIJ(MisCB). (275 aa) |
| | yqjB | Conserved hypothetical protein; Evidence 4: Homologs of previously reported genes of unknown function. (176 aa) |
| | spo0A | Response regulator; May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process. Repressor of abrB, activator of the spoIIa operon. Binds the DNA sequence 5'-TGNCGAA-3' (0A box). (267 aa) |
| | ahrC | Transcriptional regulator; Represses the synthesis of biosynthetic enzymes and activates the arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF. (149 aa) |
| | yqhH | Putative RNA polymerase-associated helicase protein; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme; Belongs to the SNF2/RAD54 helicase family. (557 aa) |
| | pbpA | Transpeptidase (penicillin-binding protein 2A); Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme. (716 aa) |
| | cshB | ATP-dependent RNA helicase; DEAD-box RNA helicase that plays a role in 70S ribosome assembly. May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures. (438 aa) |
| | dnaG | DNA primase; RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. (603 aa) |
| | era | GTP-binding protein; An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism (By similarity). Binds both GDP and GTP. Complements an E.coli era disruption mutant; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family. (301 aa) |
| | rsfS | Ribosomal silencing factor; Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation. (118 aa) |
| | cwlH | N-acetylmuramoyl-L-alanine amidase; Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC; Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. (250 aa) |
| | cwlA | N-acetylmuramoyl-L-alanine amidase; Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation; Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. (272 aa) |
| | yrpC | Glutamate racemase; Provides the (R)-glutamate required for cell wall biosynthesis. (265 aa) |
| | pbpI | Penicillin-binding protein PBP4B; Penicillin-binding protein with an unknown catalytic activity. Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active site of these proteins, thereby interrupting normal cell wall synthesis; Belongs to the transpeptidase family. (584 aa) |
| | yrrL | Conserved hypothetical protein; Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation. Belongs to the transglycosylase MltG family. (360 aa) |
| | recD | Exodeoxyribonuclease V alpha chain; DNA-dependent ATPase and ATP-dependent 5'-3' DNA helicase. Has no activity on blunt DNA or DNA with 3'-overhangs, requires at least 10 bases of 5'-ssDNA for helicase activity; Belongs to the RecD family. RecD-like subfamily. (798 aa) |
| | iscSA | Cysteine desulfurase involved in tRNA thiolation; Catalyzes the removal of elemental sulfur from cysteine to produce alanine. (379 aa) |
| | cymR | Transcriptional regulator of cysteine biosynthesis; Master repressor of cysteine metabolism in B.subtilis. Controls the expression of genes involved either in cysteine synthesis from sulfide (cysK), sulfonates (ssu), or methionine (mccAB) or in cystine uptake (tcyP). Activity of CymR is positively regulated by CysK in response to cysteine availability. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR-CysK complex and transcriptional repression of the CymR regulon occurs. In the absence of cysteine, the OAS pool is high and the Cy [...] (138 aa) |
| | rarA | DNA-dependent ATPase; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily. (421 aa) |
| | yrvJ | Putative N-acetylmuramoyl-L-alanine amidase, family 3; Probably involved in cell-wall metabolism. (518 aa) |
| | ruvB | Holliday junction DNA helicase, ATP-dependent component; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing (By similarity). Stimulates the resolution of Holliday junctions by RecU. (334 aa) |
| | ruvA | Holliday junction DNA helicase; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. (201 aa) |
| | minD | ATPase activator of MinC; ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings (By similarity); Belongs to the ParA family. MinD subfamily. (268 aa) |
| | minC | Cell-division regulator (septum placement); Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization. The MinCD complex plays an important role in asymmetric septum formation during sporulation of B.subtilis cells. (226 aa) |
| | hemX | Negative effector of the concentration of glutamyl-tRNA reductase HemA; Required for HemL synthesis; To M.leprae U1620K. (276 aa) |
| | ysxC | GTPase involved in ribosome 50S subunit assembly; Necessary for normal cell division and for the maintenance of normal septation; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family. (195 aa) |
| | racE | Glutamate racemase; Provides the (R)-glutamate required for cell wall biosynthesis. (272 aa) |
| | yshA | Conserved hypothetical protein; Activator of cell division through the inhibition of FtsZ GTPase activity, therefore promoting FtsZ assembly into bundles of protofilaments necessary for the formation of the division Z ring. It is recruited early at mid-cell but it is not essential for cell division (By similarity); Belongs to the ZapA family. Type 2 subfamily. (85 aa) |
| | rplT | Ribosomal protein L20; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity). (119 aa) |
| | infC | Initiation factor IF-3; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (173 aa) |
| | lytS | Two-component sensor histidine kinase [LytT]; Member of the two-component regulatory system LytS/LytT that probably regulates genes involved in cell wall metabolism. (593 aa) |
| | sspA | Small acid-soluble spore protein (alpha-type SASP); SASP are bound to spore DNA. They are double-stranded DNA- binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light. (69 aa) |
| | ezrA | Negative regulator of FtsZ ring formation; Negative regulator of FtsZ ring formation; modulates the frequency and position of FtsZ ring formation. Inhibits FtsZ ring formation at polar sites. Interacts either with FtsZ or with one of its binding partners to promote depolymerization. (562 aa) |
| | murC | UDP-N-acetyl muramate-alanine ligase; Cell wall formation; Belongs to the MurCDEF family. (432 aa) |
| | ytgP | Putative enzyme involved in polysaccharide biosynthesis; Involved in peptidoglycan biosynthesis. Transports lipid- linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane. Not essential for growth. (544 aa) |
| | ytcA | Putative UDP-glucose dehydrogenase; Catalyzes the conversion of UDP-glucose into UDP-glucuronate, one of the precursors of teichuronic acid. (428 aa) |
| | lytG | Exoglucosaminidase; Is the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Catalyzes the hydrolysis of 1,4-beta-linkages between N-acetyl-D-glucosamine and N- acetylmuramic acid residues in peptidoglycan. Acts processively from the ends of the glycan strands. Also plays a role in motility, chemotaxis and cell division. (282 aa) |
| | uppP | Undecaprenyl-diphosphatase; Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin; Belongs to the UppP family. (276 aa) |
| | pbpD | Penicillin-binding protein 4; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; In the C-terminal section; belongs to the transpeptidase family. (624 aa) |
| | sufA | Chaperone involved in Fe-S cluster assembly; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; factor. (120 aa) |
| | yutI | Putative iron-sulfur scaffold protein; May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins. (111 aa) |
| | lytH | Sporulation-specific L-Ala-D-Glu endopeptidase; L-Ala--D-Glu endopeptidase involved in production of single L-alanine side chains from tetrapeptides in the spore cortex peptidoglycan. Therefore, is required for the endospore cortex maturation. (326 aa) |
| | sufB | FeS cluster formation protein; The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation (By similarity). Belongs to the UPF0051 (ycf24) family. (465 aa) |
| | sufU | Iron-sulfur cluster assembly scaffold protein; Its function is controversial. Has been generally assumed to be an iron-sulfur cluster assembly scaffold protein , but more recent evidence suggest it is a sulfurtransferase rather than a scaffold assembly protein. Has been shown to bind low levels of a labile, air- sensitive Fe-S cluster; this can be assembled under anaerobic conditions from FeCl(3) and Li(2)S. Has been shown to be able to transfer this Fe-S cluster to an acceptor protein. Stimulates the cysteine desulfurase activity of SufS, for which it acts as a second substrate. Alkyl [...] (147 aa) |
| | sufD | FeS assembly protein SufD; The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation (By similarity). Belongs to the UPF0051 (ycf24) family. (437 aa) |
| | helD | DNA 3'-5' helicase IV; Catalyzes the unwinding of duplex DNA in the 3' to 5' direction; this reaction is dependent on the hydrolysis of ATP. (774 aa) |
| | pbpE | Penicillin-binding protein 4*; Probably involved in peptidoglycan modification during cortex synthesis. (451 aa) |
| | cwlO | Secreted cell wall DL-endopeptidase; The C-terminal part of CwlO shows a cell wall hydrolytic DL- endopeptidase activity; Belongs to the peptidase C40 family. (473 aa) |
| | minJ | Topological determinant of cell division; The main function of the Min system is to promote the disassembly of the cytokinetic ring after cell division, thereby ensuring that division occurs only once per cell cycle. MinJ acts as a bridge between DivIVA and MinD. May modulate activity and localization of MinD and MinC through direct interaction with MinD. (397 aa) |
| | ftsX | Cell-division ABC transporter; Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation. May act as an importer, possibly at the top of a hierarchical cascade leading to the correct temporal initiation of sporulation. Acts upstream of the histidine kinases KinA, KinB and KinC, the RapA phosphatase and the Spo0A sporulation protein; Belongs to the ABC-4 integral membrane protein family. FtsX subfamily. (296 aa) |
| | ftsE | Cell-division ABC transporter (ATP-binding protein); Part of the ABC transporter FtsEX involved in sporulation. May act as an importer, possibly at the top of a hierarchical cascade leading to the correct temporal initiation of sporulation. Acts upstream of the histidine kinases KinA, KinB and KinC, the RapA phosphatase and the Spo0A sporulation protein. (228 aa) |
| | prfB | Peptide chain release factor 2; Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (366 aa) |
| | fliT | Flagellar assembly protein FliT involved in control of flagella expression; May act as an export chaperone for the filament capping protein FliD; Belongs to the bacillales FliT family. (113 aa) |
| | priA | Primosomal replication factor Y (primosomal protein N'); Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA; Belongs to the helicase family. PriA subfamily. (805 aa) |
| | fliW | Assembly factor of the flagellum; Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin. Binds to flagellin (hag), which is implicated in polymerization, and participates in the assembly of the flagellum. An antagonist to translational regulator CsrA, it binds CsrA at an allosteric site and non-competitively inhibits CsrA binding to hag RNA. Partner switching by flagellin between FliW and CsrA provides a flagellar assembly checkpoint to tightly control the timing of flagellin synthesis. Flagellin binds to ass [...] (143 aa) |
| | flgK | Flagellar hook-filament junction; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; structure. (507 aa) |
| | yvyG | Putative flagellar protein; May be involved in the assembly, structure, or function of the flagellum. May polymerize to form a filamentous structure that is part of the flagellum. (160 aa) |
| | oxaAA | Sec-independent factor for membrane protein insertion (YidC/SpoIIIJ family); Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins (By similarity). Also involved in protein secretion processes. Essential for sporulation by activating sigma factor SpoIIIG/SigG after engulfment is completed in the prespore, maybe by acting on SpoIIIAE. It has an overlapping, al [...] (261 aa) |
| | jag | SpoIIIJ-associated RNA/ssDNA-binding protein; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type f: factor. (208 aa) |
| | noc | DNA-binding protein Spo0J-like; Effects nucleoid occlusion by binding relatively nonspecifically to DNA and preventing the assembly of the division machinery in the vicinity of the nucleoid, especially under conditions that disturb the cell cycle. It helps to coordinate cell division and chromosome segregation by preventing the formation of the Z ring through the nucleoid, which would cause chromosome breakage. (283 aa) |
| | dnaC | Replicative DNA helicase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the helicase family. DnaB subfamily. (454 aa) |
| | deaD | ATP-dependent RNA helicase; DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes (Probable). (479 aa) |
| | dltC | D-alanyl carrier protein; Carrier protein involved in the D-alanylation of lipoteichoic acid (LTA). The loading of thioester-linked D-alanine onto DltC is catalyzed by D-alanine--D-alanyl carrier protein ligase DltA. The DltC- carried D-alanyl group is further transferred to cell membrane phosphatidylglycerol (PG) by forming an ester bond, probably catalyzed by DltD. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall. (78 aa) |
| | rodA | Factor involved in extension of the lateral walls of the cell; Peptidoglycan polymerase that is essential for cell wall elongation. Also required for the maintenance of the rod cell shape. Belongs to the SEDS family. MrdB/RodA subfamily. (393 aa) |
| | pbpG | Sporulation specific penicillin-binding protein; Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane; In the C-terminal section; belongs to the transpeptidase family. (691 aa) |
| | narJ | Nitrate reductase molybdenum cofactor assembly chaperone NarJ; Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase. Belongs to the NarJ/NarW family. (184 aa) |
| | murAB | UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine; Belongs to the EPSP synthase family. MurA subfamily. (429 aa) |
| | racA | Chromosome-anchoring protein RacA; Required for the formation of axial filaments and for anchoring the origin regions at the cell poles in sporulating cells, thus ensuring proper chromosome segregation in the prespore. Binds in a dispersed manner throughout the chromosome but preferentially to sites clustered in the origin portion of the chromosome, causing condensation of the chromosome and its remodeling into an elongated, anchored structure; Belongs to the RacA family. (184 aa) |
| | prfA | Peptide chain release factor 1; Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. (356 aa) |
| | murAA | UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine. Essential for cell growth; Belongs to the EPSP synthase family. MurA subfamily. (436 aa) |
| | bcrC | Undecaprenyl pyrophosphate phosphatase; Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin; Belongs to the BcrC/YbjG family. (193 aa) |
| | flhO | Putative flagellar basal-body rod protein; Not required for motility. (270 aa) |
| | flhP | Putative flagellar hook-basal body protein; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type ps: putative structure; Belongs to the flagella basal body rod proteins family. (269 aa) |
| | mscL | Large conductance mechanosensitive channel protein; Channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. May participate in the regulation of osmotic pressure changes within the cell. (130 aa) |
| | hepA | ATPase involved in RNA remodelling DNA recombination and repair; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type f: factor; Belongs to the SNF2/RAD54 helicase family. (922 aa) |
| | ywqC | Modulator of YwqD protein tyrosine kinase activity; Required for YwqD kinase activity. May bring YwqD and its substrates into contact. Probably involved in the regulation of capsular polysaccharide biosynthesis. (248 aa) |
| | ywqF | UDP-glucose dehydrogenase; Catalyzes the conversion of UDP-glucose into UDP-glucuronate, one of the precursors of teichuronic acid. Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. (440 aa) |
| | capB | Capsular polyglutamate synthetase (ATP-dependent amide ligase); Catalyzes the biosynthesis of PGA (gamma-polyglutamic acid) from L-glutamate. Both the 44-kDa and the 33-kDa forms are required for PGA synthesis. (393 aa) |
| | capC | Capsular polyglutamate amide ligase/translocase subunit; Required for PGA (gamma-polyglutamic acid) biosynthesis. (149 aa) |
| | ywtF | Putative transcriptional regulator; May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG). (322 aa) |
| | lytD | Exported N-acetylglucosaminidase (major autolysin) (CWBP90); Cell wall hydrolase not involved in cell autolysis, competence, sporulation or germination. It hydrolyzes the beta-1,4 glycan bond between the N-acetylglucosaminyl and the N-acetylmuramoyl residues in the glycan chain. (880 aa) |
| | tagC | Putative polyglycerol phosphate assembly and export protein (teichoic acid biosynthesis); Unknown. Might be involved in poly(glycerol phosphate) teichoic acid biosynthesis. (442 aa) |
| | tagB | Teichoic acid glycerol-phosphate primase; Catalyzes the addition of a single glycerol phosphate residue to the prenoldiphosphate-linked disaccharide, as a primer for polymerisation by TagF; Belongs to the CDP-glycerol glycerophosphotransferase family. (381 aa) |
| | tagA | N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase; Catalyzes the conversion of GlcNAc-PP-undecaprenol into ManNAc-GlcNAc-PP-undecaprenol, the first committed lipid intermediate in the de novo synthesis of teichoic acid. Belongs to the glycosyltransferase 26 family. TagA/TarA subfamily. (256 aa) |
| | tagD | Glycerol-3-phosphate cytidylyltransferase; Catalyzes the transfer of the cytidylyl group of CTP to sn- glycerol 3-phosphate so the activated glycerol 3-phosphate can be used for teichoic acid synthesis, via incorporation into both the linkage unit and the teichoic acid polymer by TagB and TagF. Belongs to the cytidylyltransferase family. (129 aa) |
| | tagE | UDP-glucose:polyglycerol phosphate alpha-glucosyltransferase; Catalyzes the addition of glucose to the C-2 hydroxy group of the glycerol units in teichoic acid. (673 aa) |
| | tagF | Teichoic acid poly(glycerol phosphate) polymerase; Responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the disaccharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in length. (746 aa) |
| | ggaA | Poly(glucosyl N-acetylgalactosamine 1-phosphate) glucosyltransferase; Involved in the biosynthesis of galactosamine-containing minor teichoic acid, a non-essential cell wall polymer in B.subtilis 168; Belongs to the glycosyltransferase 2 family. (446 aa) |
| | ggaB | Putative teichoic acid translocation permease protein tagG (fragment); Involved in the biosynthesis of galactosamine-containing minor teichoic acid, a non-essential cell wall polymer in B.subtilis 168; Belongs to the glycosyltransferase 2 family. (900 aa) |
| | mnaA | UDP-N-acetylmannosamine 2-epimerase; Catalyzes the conversion of UDP-N-acetylglucosamine into UDP- N-acetylmannosamine, a precursor of the teichoic acid linkage unit. (380 aa) |
| | lytR | Membrane-bound transcriptional regulator; May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG). (306 aa) |
| | lytB | Modifier protein of major autolysin LytC; Possibly involved in cell wall metabolism during spore formation. Enhances the amidase activity approximately threefold. (705 aa) |
| | lytC | Putative undecaprenyl-phosphate N-acetylgalactosaminyl-1-phosphate transferase; Autolysins are cell wall hydrolases involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teichoic acid-endowed peptidoglycan. LytC is required for efficient swarming motility but not at the level of cell separation or flagellum biosynthesis. Rather, LytC appears to be important for proper flagellar function. (496 aa) |
| | tuaB | Putative exporter involved in biosynthesis of teichuronic acid; Might be involved in the translocation of teichuronic acid repeating units from the inner to the outer surface of the membrane. (483 aa) |
| | tuaC | Putative glycosyltransferase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme. (389 aa) |
| | tuaD | UDP-glucose 6-dehydrogenase; Catalyzes the conversion of UDP-glucose into UDP-glucuronate, one of the precursors of teichuronic acid; Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. (461 aa) |
| | tuaE | Putative polymerase of teichuronic acid repeating units; Might be involved in the polymerization of teichuronic acid repeating units after their translocation to the outer surface of the membrane. (488 aa) |
| | tuaF | Putative hydrolase involved in teichuronic acid synthesis; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme. (226 aa) |
| | tuaG | Putative glycosyltransferase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme. (252 aa) |
| | tuaH | Putative glycosyltransferase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme. (397 aa) |
| | tagO | UDP-N-acetylglucosamine:undecaprenyl-P N-acetylglucosaminyl-1-P transferase; Catalyzes the formation of undecaprenyl-PP-N- acetylglucosamine. Involved in the synthesis of anionic cell-wall polymers as it mediates the initiation of the linkage unit formation that appears to be common to the two types of teichoic acids attached to the peptidoglycan of B.subtilis; may also be involved in teichuronic acid biosynthesis (Probable); Belongs to the glycosyltransferase 4 family. (358 aa) |
| | yvhJ | Putative membrane bound transcriptional regulator; May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG). Belongs to the LytR/CpsA/Psr (LCP) family. (391 aa) |
| | comFA | Helicase competence protein; Involved in transformation (competence for DNA uptake). Required for DNA uptake but not for binding. May provide the driving force for transport of DNA through an aqueous channel. Belongs to the helicase family. (463 aa) |
| | yvyF | Putative regulator of flagella formation; May be involved in the assembly, structure, or function of the flagellum. May polymerize to form a filamentous structure that is part of the flagellum. (139 aa) |
| | flgM | Anti-sigma factor repressor of sigma(D)-dependent transcription; Allows the coupling of early and late flagellar synthesis through the repression of RNA polymerase sigma-D factor-dependent transcription. (88 aa) |
| | gyrB | DNA gyrase (subunit B); A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (638 aa) |
| | gyrA | DNA gyrase (subunit A); A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (821 aa) |
| | dacA | D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5); Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors; Belongs to the peptidase S11 family. (443 aa) |
| | sspF | Small acid-soluble spore protein (alpha/beta-type SASP); May play some important role in either sporulation or the dormant spore. (61 aa) |
| | spoVG | Regulator required for spore cortex synthesis (stage V sporulation); Essential for sporulation. Interferes with or is a negative regulator of the pathway leading to asymmetric septation. Belongs to the SpoVG family. (97 aa) |
| | gcaD | Bifunctional glucosamine-1-phosphate N-acetyltransferase/UDP-N-acetylglucosamine pyrophosphorylase; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. In the C-terminal section; belon [...] (456 aa) |
| | mfd | Transcription-repair coupling factor; Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site; In the N-terminal section; belongs to the UvrB family. (1177 aa) |
| | rpsG | Ribosomal protein S7 (BS7); One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | fusA | Elongation factor G; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity). (692 aa) |
| | rplW | Ribosomal protein L23; One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome; Belongs to the universal ribosomal protein uL23 family. (95 aa) |
| | rpsS | Ribosomal protein S19 (BS19); Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (92 aa) |
| | rplV | Ribosomal protein L22 (BL17); This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (113 aa) |
| | rpsK | Ribosomal protein S11 (BS11); Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (131 aa) |
| | cwlD | N-acetylmuramoyl-L-alanine amidase; Cleaves the peptide side chain from the N-acetylmuramic acid residues in peptidoglycan. This is a step in the formation of muramic delta-lactam residues in spore cortex. (237 aa) |
| | salA | Mrp family regulator; Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP; Belongs to the Mrp/NBP35 ATP-binding proteins family. (352 aa) |
| | nagZ | N-acetylglucosaminidase lipoprotein; Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide- linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N- acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides. Cleaves muropeptides, but not peptidoglycan. Belongs to the glycosyl hydrolase 3 family. (642 aa) |
| | amiE | Amidase hydrolyzing N-acetylmuramyl-L-Ala bond of MurNAc peptides; Involved in muropeptide recycling. Hydrolyzes the amide bond between N-acetylmuramic acid (MurNAc) and the L-alanine residue of the stem peptide. Cannot hydrolyze muropeptides containing N- acetylglucosamine (GlcNAc) at the non-reducing end. (441 aa) |
| | ybxI | Exported beta-lactamase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme. (267 aa) |
| | cwlJ | Cell wall hydrolase; Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination. (142 aa) |
| | cwlK | Murein L,D:-endopeptidase; Cleaves the linkage of the L-alanine-D-glutamic acid of B.subtilis cell wall; Belongs to the peptidase M15C family. (167 aa) |
| | yciB | Putative metal uptake system lipoprotein; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pt: putative transporter. (194 aa) |
| | pbpC | Penicillin-binding lipoprotein 3; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type lp: lipoprotein. (668 aa) |
| | ydaH | Putative integral inner membrane protein; Involved in peptidoglycan biosynthesis. Transports lipid- linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane. May serve as a defense mechanism against naturally occurring MurJ antagonists. (269 aa) |
| | topB | DNA topoisomerase III; Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA su [...] (727 aa) |
| | ddl | D-alanyl-D-alanine ligase A; Cell wall formation. (354 aa) |
| | murF | UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanine ligase; Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein; Belongs to the MurCDEF family. MurF subfamily. (457 aa) |
| | cshA | ATP-dependent RNA helicase; The most abundant DEAD-box RNA helicase. An ATP-dependent RNA helicase with RNA-dependent ATPase activity. May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures. In vitro, unwinds dsRNA in both 5'- and 3'- directions. Plays a role in ribosomal 50S subunit assembly. Its deletion leads to changes in mRNA levels for over 200 transcripts. (494 aa) |
| | pcrA | ATP-dependent DNA helicase; DNA helicase used for plasmid rolling-circle replication and also involved in UV repair. (739 aa) |
| | rhgW | Rhamnogalacturonan endolyase; Pectinolytic enzyme that degrades type I rhamnogalacturonan from plant cell walls and releases oligosaccharide products. Degrades rhamnogalacturonan, polygalacturonic acid, pectic acid and pectin ; Belongs to the polysaccharide lyase 11 family. (620 aa) |
| | rhgX | Rhamnogalacturonan exolyase YesX; Pectinolytic enzyme that degrades type I rhamnogalacturonan from plant cell walls and releases disaccharide products. Degrades rhamnogalacturonan, polygalacturonic acid and pectic acid. Has very low activity on pectin ; Belongs to the polysaccharide lyase 11 family. (612 aa) |
| | ltaSA | Exported glycerol phosphate lipoteichoic acid synthetase and anion-binding protein; Catalyzes the polymerization of lipoteichoic acid (LTA) polyglycerol phosphate, a reaction that presumably uses phosphatidylglycerol (PG) as substrate. (639 aa) |
| | yfmL | Putative ATP-dependent RNA helicase; A probable DEAD-box RNA helicase that plays a role in ribosomal 50S subunit assembly. May be a non-specific RNA helicase. Belongs to the DEAD box helicase family. (376 aa) |
| | ltaSB | Enzyme responsible for polyglycerolphosphate LTA synthesis; Catalyzes the polymerization of lipoteichoic acid (LTA) polyglycerol phosphate, a reaction that presumably uses phosphatidylglycerol (PG) as substrate; Belongs to the LTA synthase family. (649 aa) |
| | pdaA | Exported N-acetylmuramic acid deacetylase; Catalyzes the deacetylation of N-acetylmuramic acid (MurNAc) residues in glycan strands of peptidoglycan, leading to the formation of muramic delta-lactam residues in spore cortex, after transpeptidation of deacetylated muramic acid residues. PdaA probably carries out both deacetylation and lactam ring formation and requires the product of CwlD activity on peptidoglycan as a substrate. Is required for germination. Cannot use chitin oligomer (hexa-N- acetylchitohexaose) as a substrate; Belongs to the polysaccharide deacetylase family. (263 aa) |
| | yhcK | Putative diguanylate cyclase or phosphodiesterase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme. (359 aa) |
| | lytF | gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin); Cell wall hydrolase that cleaves gamma-D-glutamate-meso- diaminopimelate bonds in peptidoglycan. LytF is necessary and sufficient for vegetative daughter cell separation, and also seems to play a role in cell autolysis. (488 aa) |
| | lytE | Cell wall hydrolase; Cell wall hydrolase that cleaves gamma-D-glutamate-meso- diaminopimelate bonds in peptidoglycan (By similarity). Seems to play a role in cell separation during vegetative growth. (334 aa) |
| | sspB | Small acid-soluble spore protein (beta-type SASP); SASP are bound to spore DNA. They are double-stranded DNA- binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light. (67 aa) |
| | pbpF | Penicillin-binding protein 2C required for spore germination; Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall; In the N-terminal section; belongs to the glycosyltransferase 51 family. (714 aa) |
| | addB | ATP-dependent deoxyribonuclease (subunit B); The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent single-stranded exonuclease, acting in both directions. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' to 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddB nuclease domain is not required for chi fragment generation; this s [...] (1166 aa) |
| | addA | ATP-dependent deoxyribonuclease (subunit A); An essential component of the DNA double-stranded break repair machinery, the heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' -> 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddA nucl [...] (1232 aa) |
| | wprA | Cell wall-associated protease; CWBP52 is a serine-type protease that could be involved in proteoglycan peptide bridges; Belongs to the peptidase S8 family. (894 aa) |
| | yjcD | Putative ATP-dependent DNA helicase; May be involved in the generation of recombinogenic substrates for the subsequent action of RecA. (759 aa) |
| | xlyB | N-acetylmuramoyl-L-alanine amidase; Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. (317 aa) |
| | xtmA | PBSX defective prophage terminase (small subunit); Functions as a terminase; To B.subtilis YqaS and B.subtilis phage SPP1 terminase small subunit. (265 aa) |
| | xlyA | Bacteriophage PBSX N-acetylmuramoyl-L-alanine amidase; Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. (297 aa) |
| | ldcA | Muropeptide L,D-carboxypeptidase; May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides; Belongs to the peptidase S66 family. (319 aa) |
| | ykfB | L-Ala-D/L-Glu epimerase; Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L-Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipeptides L-Ala-L- Asp, L-Ala-L-Leu, L-Ala-L-Met, and L-Ala-L-Ser. Is not able to epimerize other L-Ala-X dipeptides. Is also active with L-Ser-L-Glu and, oddly, L-Pro-L-Glu, but not with L-Glu-L-Glu, L-Lys-L-Glu, L-Lys- L-Ala, or D-Ala-D-Ala. (366 aa) |
| | ykfC | gamma-D-glutamyl-L-diaminoacid endopeptidase; Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu. (296 aa) |
| | sspD | Small acid-soluble spore protein (alpha/beta-type SASP); SASP are bound to spore DNA. They are double-stranded DNA- binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light. (64 aa) |
| | pbpH | Penicillin-binding enzyme for formation of rod-shaped peptidoglycan cell wall; Involved in the polymerization of peptidoglycan. Plays a redundant role with PBP2a in determining the rod shape of the cell during vegetative growth and spore outgrowth. Belongs to the transpeptidase family. (704 aa) |
| | ykuD | Murein transglycosylase; Probable enzyme that may play an important role in cell wall biology; Belongs to the YkuD family. (164 aa) |
| | bipA | GTPase; A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily. (612 aa) |
| | ftsW | Cell-division protein; Peptidoglycan polymerase that is essential for cell division. (403 aa) |
| | pbpB | Penicillin-binding protein 2B; Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active site of these proteins, thereby interrupting normal cell wall synthesis; Belongs to the transpeptidase family. (716 aa) |
| | spoVD | Penicillin-binding protein; Penicillin-binding protein with an unknown catalytic activity. May have a specialized role in the morphogenesis of spore cortex, which is a modified form of peptidoglycan. Pore cortex formation is determined primarily by the mother cell. (646 aa) |
| | murE | UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase; Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. Belongs to the MurCDEF family. MurE subfamily. (494 aa) |
| | mraY | phospho-N-acetylmuramoyl-pentapeptide transferase; First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan; Belongs to the glycosyltransferase 4 family. MraY subfamily. (324 aa) |
| | murD | UDP-N-acetylmuramoylalanyl-D-glutamate ligase; Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA). Belongs to the MurCDEF family. (451 aa) |
| | murG | Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase; Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II); Belongs to the glycosyltransferase 28 family. MurG subfamily. (363 aa) |
| | murB | UDP-N-acetylenolpyruvoylglucosamine reductase; Cell wall formation. (303 aa) |
| | ftsZ | Cell-division initiation protein; Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity. (382 aa) |
| | sepF | Cell division machinery factor; Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA; Belongs to the SepF family. (151 aa) |
| | divIVA | Cell-division initiation protein; May act as a pilot protein, directing MinCD to the polar septation sites or by inhibiting MinCD at the midcell site of division. Required for polar localization of the chromosome during sporulation. (164 aa) |
