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| | gntZ | NAD+-6-phosphogluconate dehydrogenase; Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NAD to NADH. Does not contribute to oxidative pentose phosphate (PP) pathway fluxes during growth on glucose. The functional role of GntZ remains obscure. (468 aa) |
| | pdxT | Glutamine amidotransferase for pyridoxal phosphate synthesis; Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS. (196 aa) |
| | cysK | Cysteine synthase; Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR- CysK complex and transcriptional repression of the CymR regulon occurs. In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster dissociation of CymR from its DNA targets and the lifting of CymR-dependent repression. (308 aa) |
| | cysE | Serine acetyltransferase; Catalyzes the acetylation of serine by acetyl-CoA to produce O-acetylserine (OAS). (217 aa) |
| | murQ | D-lactyl ether N-acetylmuramic-6-phosphate acid etherase; Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D- lactate. (304 aa) |
| | nagBB | Glucosamine-6-phosphate isomerase; Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion; Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily. (249 aa) |
| | glsA | Glutaminase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme. (327 aa) |
| | ycbC | 5-dehydro-4-deoxyglucarate dehydratase; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; Belongs to the DapA family. (308 aa) |
| | gudD | Glucarate dehydratase; Catalyzes the dehydration of glucarate to 5-keto-4-deoxy-D- glucarate (5-kdGluc); Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily. (455 aa) |
| | garD | D-galactarate dehydratase; Catalyzes the dehydration of galactarate to form 5-dehydro-4- deoxy-D-glucarate. (510 aa) |
| | ansZ | L-asparaginase 2 (putative lipoprotein); Catalyzes the conversion of L-asparagine to L-aspartate and ammonium. (375 aa) |
| | putB | Proline oxidase; Converts proline to delta-1-pyrroline-5-carboxylate. Important for the use of proline as a sole carbon and energy source or a sole nitrogen source. (303 aa) |
| | putC | 1-pyrroline-5-carboxylate dehydrogenase; Important for the use of proline as a sole carbon and energy source or a sole nitrogen source. (515 aa) |
| | gabT | 4-aminobutyrate aminotransferase; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. (436 aa) |
| | gabD | Succinate-semialdehyde dehydrogenase; Catalyzes the NADP(+) dependent oxidation of succinate semialdehyde to succinate. (462 aa) |
| | gabP | Gamma-aminobutyrate (GABA) permease; High-affinity uptake system for GABA. Functions also as a low-affinity proline importer; Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family. (469 aa) |
| | dat | D-alanine aminotransferase; Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha-keto acid in the first half-reaction. The second half-reaction is the reverse of the first, transferring the amino group from the pyridoxamine to a second alpha-keto acid to form the product D-amino acid via a ping-pong mechanism. This is an important process in the formation of D-alanine and D-glutamate, which are essen [...] (282 aa) |
| | lcfB | Long-chain fatty-acid-CoA ligase (degradative); Involved in the degradation of long-chain fatty acids; Belongs to the ATP-dependent AMP-binding enzyme family. (513 aa) |
| | yhfS | Putative acetyl-CoA C-acetyltransferase; May be involved in fatty acid metabolism; Belongs to the thiolase-like superfamily. Thiolase family. (364 aa) |
| | uxaC | Galacturonate isomerase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme. (473 aa) |
| | uxuA | D-mannonate dehydratase; Catalyzes the dehydration of D-mannonate; Belongs to the mannonate dehydratase family. (359 aa) |
| | uxaB | Tagaturonate reductase (altronate oxidoreductase); Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; Belongs to the mannitol dehydrogenase family. UxaB subfamily. (480 aa) |
| | uxaA | Altronate hydrolase; Catalyzes the dehydration of D-altronate; Belongs to the UxaA family. (497 aa) |
| | ykgA | Putative aminohydrolase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme; Belongs to the DDAH family. (286 aa) |
| | fadH | Putative 2,4-dienoyl-CoA reductase; Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA (By similarity); Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily. (254 aa) |
| | glsB | Glutaminase; Evidence 2b: Function of strongly homologous gene; Product type e: enzyme. (309 aa) |
| | coaBC | Coenzyme A biosynthesis bifunctional protein CoaBC; Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'- phosphopantotheine; In the C-terminal section; belongs to the PPC synthetase family. (406 aa) |
| | tdh | Threonine 3-dehydrogenase; Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2- amino-3-ketobutyrate; Belongs to the zinc-containing alcohol dehydrogenase family. (347 aa) |
| | pksI | Decarboxylase involved in polyketide synthesis; Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3- methylglutaryl group attached to PksL. (249 aa) |
| | yngE | Putative methylcrotonoyl-CoA carboxylase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative enzyme; Belongs to the AccD/PCCB family. (511 aa) |
| | yngF | Putative Methylglutaconyl-CoA hydratase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative enzyme; Belongs to the enoyl-CoA hydratase/isomerase family. (260 aa) |
| | yngG | Putative hydroxymethylglutaryl-CoA lyase; Involved in the catabolism of branched amino acids such as leucine; Belongs to the HMG-CoA lyase family. (299 aa) |
| | yngJ | acyl-CoA dehydrogenase, short-chain specific; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; Belongs to the acyl-CoA dehydrogenase family. (380 aa) |
| | yoaI | Putative 4-hydroxyphenylacetate-3-hydroxylase; Catalyzes the hydroxylation of 4-hydroxyphenylacetic acid (4HPA), leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA). (483 aa) |
| | yobN | Putative amine oxidase; Evidence 3: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; Product type pe: putative enzyme; Belongs to the flavin monoamine oxidase family. FIG1 subfamily. (478 aa) |
| | odhB | 2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit); E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2- oxoglutarate to succinyl-CoA and CO(2). (417 aa) |
| | kamA | Lysine 2,3-aminomutase; Catalyzes the interconversion of L-alpha-lysine and L-beta- lysine; Belongs to the radical SAM superfamily. KamA family. (471 aa) |
| | ilvA | Threonine dehydratase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity). (422 aa) |
| | kduI | 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase, 5-keto-4-deoxyuronate isomerase; Catalyzes the isomerization of 5-dehydro-4-deoxy-D- glucuronate to 3-deoxy-D-glycero-2,5-hexodiulosonate. (275 aa) |
| | gudB | Cryptic glutamate dehydrogenase; GudB seems to be intrinsically inactive, however spontaneous mutations removing a 9-bp direct repeat within the wild-type gudB sequence activated the GudB protein and allowed more-efficient utilization of amino acids of the glutamate family. This insertion presumably causes severe destabilization of the fold of the protein, leading to an inactive enzyme that is very quickly degraded. The cryptic GudB serves as a buffer that may compensate for mutations in the rocG gene and that can also be decryptified for the utilization of glutamate as a single carbon [...] (427 aa) |
| | dsdA | D-serine ammonia-lyase; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; Belongs to the serine/threonine dehydratase family. DsdA subfamily. (448 aa) |
| | gndA | NADP+-dependent 6-P-gluconate dehydrogenase; Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH. Is the predominant 6-P-gluconate dehydrogenase isoenzyme in B.subtilis during growth on glucose and gluconate. (469 aa) |
| | bkdAB | Branched-chain alpha-keto acid dehydrogenase E1 subunit; The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). (327 aa) |
| | bkdAA | Branched-chain alpha-keto acid dehydrogenase E1 subunit; The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3); Belongs to the BCKDHA family. (330 aa) |
| | bcd | Branched-chain amino acid dehydrogenase; Catalyzes the reversible deamination of L-leucine to 4- methyl-2-oxopentanoate. (364 aa) |
| | mmgF | 2-methylisocitrate lyase; Involved in the methylcitric acid cycle. Catalyzes the cleavage of 2-methylisocitrate to yield pyruvate and succinate. (301 aa) |
| | mmgC | Short chain acyl-CoA dehydrogenase; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme; Belongs to the acyl-CoA dehydrogenase family. (379 aa) |
| | mmgA | Degradative acetoacetyl-CoA thiolase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme; Belongs to the thiolase-like superfamily. Thiolase family. (393 aa) |
| | ahrC | Transcriptional regulator; Represses the synthesis of biosynthetic enzymes and activates the arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF. (149 aa) |
| | aroQ | 3-dehydroquinate dehydratase, type II; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme. (148 aa) |
| | gcvPB | Glycine decarboxylase (subunit 2) (glycine cleavage system protein P); The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (By similarity); Belongs to the GcvP family. C-terminal subunit subfamily. (488 aa) |
| | gcvPA | Glycine decarboxylase (subunit 1) (glycine cleavage system protein P); The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (By similarity). (448 aa) |
| | gcvT | Aminomethyltransferase (glycine cleavage system protein T); The glycine cleavage system catalyzes the degradation of glycine. (362 aa) |
| | mccB | Cystathionine gamma-lyase and homocysteine gamma-lyase for reverse transsulfuration pathway; Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide. (379 aa) |
| | argI | Arginase; Involved in the catabolism of arginine. Belongs to the arginase family. (296 aa) |
| | dtd | D-Tyr-tRNATyr deacylase; A non-functional D-aminoacyl-tRNA deacylase. (132 aa) |
| | nadC | Nicotinate-nucleotide pyrophosphorylase; Involved in the catabolism of quinolinic acid (QA). (289 aa) |
| | etfA | Electron transfer flavoprotein (alpha subunit); The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (By similarity). (325 aa) |
| | fadB | enoyl-CoA hydratase; Involved in the degradation of long-chain fatty acids. (258 aa) |
| | ald | L-alanine dehydrogenase; Catalyzes the reversible oxidative deamination of L-alanine to pyruvate. This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation. (378 aa) |
| | gcvH | Glycine cleavage system protein H; The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein. (127 aa) |
| | fadE | acyl-CoA dehydrogenase (FAD dependent); Involved in the degradation of long-chain fatty acids. (594 aa) |
| | fadA | acetyl-CoA C-acyltransferase; Involved in the degradation of long-chain fatty acids; Belongs to the thiolase-like superfamily. Thiolase family. (391 aa) |
| | fadN | enoyl-CoA hydratase / 3-hydroxyacyl-CoA dehydrogenase; Involved in the degradation of long-chain fatty acids; Belongs to the 3-hydroxyacyl-CoA dehydrogenase family. (789 aa) |
| | putM | Proline dehydrogenase 1; Converts proline to delta-1-pyrroline-5-carboxylate. (302 aa) |
| | nagA | N-acetylglucosamine-6-phosphate deacetylase; Involved in the first committed step in the biosynthesis of amino-sugar-nucleotides. Catalyzes the hydrolysis of the N-acetyl group of N-acetylglucosamine-6-phosphate (GlcNAc-6-P) to yield glucosamine 6- phosphate and acetate; Belongs to the metallo-dependent hydrolases superfamily. NagA family. (396 aa) |
| | nagBA | N-acetylglucosamine-6-phosphate isomerase; Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion; Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily. (242 aa) |
| | glyA | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism (By similarity); Belongs to the SHMT family. (415 aa) |
| | acdA | acyl-CoA dehydrogenase; Involved in the degradation of long-chain fatty acids. (379 aa) |
| | rocA | Delta-1-pyrroline-5 carboxylate dehydrogenase; Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme. (515 aa) |
| | rocG | Glutamate dehydrogenase; Devoted to catabolic function of glutamate (and other amino acids of the glutamate family) utilization as sole nitrogen source. It is not involved in anabolic function of glutamate biosynthesis since B.subtilis possesses only one route of glutamate biosynthesis from ammonia, catalyzed by glutamate synthase. RocG is unable to utilize glutamate or glutamine as sole carbon source and to synthesize glutamate, but it is involved in the utilization of arginine, and proline as carbon or nitrogen source. The catabolic RocG is essential for controlling gltAB expression [...] (424 aa) |
| | hutH | Histidine ammonia-lyase (histidase); Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; Product type e: enzyme. (508 aa) |
| | hutU | Urocanase; Catalyzes the conversion of urocanate to 4-imidazolone-5- propionate; Belongs to the urocanase family. (552 aa) |
| | hutI | Imidazolone-5-propionate hydrolase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme. (421 aa) |
| | hutG | Formiminoglutamate hydrolase; Catalyzes the conversion of N-formimidoyl-L-glutamate to L- glutamate and formamide. (319 aa) |
| | mmsA | Methylmalonate-semialdehyde dehydrogenase; Catalyzes the oxidation of malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively. (487 aa) |
| | gntK | Gluconate kinase; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type e: enzyme. (513 aa) |
| | gntP | Gluconate permease; Evidence 1a: Function experimentally demonstrated in the studied strain; Product type t: transporter; Belongs to the GntP permease family. (448 aa) |
