(8 nodes) (2010) Direct visualization by cryo-EM of the mycobacterial capsular layer: a labile structure containing ESX-1-secreted proteins. network (Mycolicibacterium smegmatis)

Nodes:

Network nodes represent proteins

splice isoforms or post-translational modifications are collapsed, i.e. each node represents all the proteins produced by a single, protein-coding gene locus.

Node Color

colored nodes:
query proteins and first shell of interactors

white nodes:
second shell of interactors

Node Content

empty nodes:
proteins of unknown 3D structure

filled nodes:
a 3D structure is known or predicted

Edges:

Edges represent protein-protein associations

associations are meant to be specific and meaningful, i.e. proteins jointly contribute to a shared function; this does not necessarily mean they are physically binding to each other.

Known Interactions

from curated databases

experimentally determined

Predicted Interactions

gene neighborhood

gene fusions

gene co-occurrence

Others

textmining

co-expression

protein homology

Your Input:
	espE	Hypothetical protein; Identified by Glimmer2; putative. (525 aa)
	groL-2	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. (540 aa)
	tuf	Translation elongation factor Tu; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa)
	groL	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. (541 aa)
	dnaK	Chaperone protein DnaK; Acts as a chaperone; Belongs to the heat shock protein 70 family. (622 aa)
	esxA	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart. (95 aa)
	eccCb1	Ftsk/spoiiie family protein; Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB. Plays a role in DNA conjugation, in both donor and recipient strains. (593 aa)
	pimE	Conserved transmembrane protein; Catalyzes the alpha-1,2 addition of a mannose residue from polyprenol-phosphate-mannose (PPM) to a monoacyl phosphatidylinositol pentamannoside (AcPIM5) to generate a monoacyl phosphatidylinositol hexamannoside (AcPIM6). (377 aa)

Your Current Organism:

Mycolicibacterium smegmatis

NCBI taxonomy Id: 246196
Other names: M. smegmatis MC2 155, Mycobacterium smegmatis MC2 155, Mycolicibacterium smegmatis MC2 155

Send network to Cytoscape

Export your current network:

... as a bitmap image:

download

file format is 'PNG': portable network graphic

... as a high-resolution bitmap:

download

same PNG format, but at higher resolution

... as a vector graphic:

download

SVG: scalable vector graphic - can be opened and edited in Illustrator, CorelDraw, Dia, etc

... as short tabular text output:

download

TSV: tab separated values - can be opened in Excel and Cytoscape (lists only one-way edges: A-B)

... as tabular text output:

download

TSV: tab separated values - can be opened in Excel (lists reciprocal edges: A-B,B-A)

... as an XML summary:

download

structured XML interaction data, according to the 'PSI-MI' data standard

... protein node degrees:

download

node degree of proteins in your network (given the current score cut-off)

... network coordinates:

download

a flat-file format describing the coordinates and colors of nodes in the network

... protein sequences:

download

MFA: multi-fasta format - containing the aminoacid sequences in the network

... protein annotations:

download

a tab-delimited file describing the names, domains and descriptions of proteins in your network

... functional annotations:

download

a tab-delimited file containing all known functional terms of proteins in your network

Browse interactions in tabular form:

node1	node2	node1 accession	node2 accession	node1 annotation	node2 annotation	score
dnaK	groL	MSMEG_0709	MSMEG_0880	Chaperone protein DnaK; Acts as a chaperone; Belongs to the heat shock protein 70 family.	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	0.992
dnaK	groL-2	MSMEG_0709	MSMEG_1583	Chaperone protein DnaK; Acts as a chaperone; Belongs to the heat shock protein 70 family.	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	0.991
dnaK	tuf	MSMEG_0709	MSMEG_1401	Chaperone protein DnaK; Acts as a chaperone; Belongs to the heat shock protein 70 family.	Translation elongation factor Tu; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.	0.850
eccCb1	espE	MSMEG_0062	MSMEG_0055	Ftsk/spoiiie family protein; Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB. Plays a role in DNA conjugation, in both donor and recipient strains.	Hypothetical protein; Identified by Glimmer2; putative.	0.953
eccCb1	esxA	MSMEG_0062	MSMEG_0066	Ftsk/spoiiie family protein; Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB. Plays a role in DNA conjugation, in both donor and recipient strains.	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	0.951
eccCb1	groL-2	MSMEG_0062	MSMEG_1583	Ftsk/spoiiie family protein; Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB. Plays a role in DNA conjugation, in both donor and recipient strains.	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	0.416
espE	eccCb1	MSMEG_0055	MSMEG_0062	Hypothetical protein; Identified by Glimmer2; putative.	Ftsk/spoiiie family protein; Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB. Plays a role in DNA conjugation, in both donor and recipient strains.	0.953
espE	esxA	MSMEG_0055	MSMEG_0066	Hypothetical protein; Identified by Glimmer2; putative.	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	0.899
esxA	eccCb1	MSMEG_0066	MSMEG_0062	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	Ftsk/spoiiie family protein; Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB. Plays a role in DNA conjugation, in both donor and recipient strains.	0.951
esxA	espE	MSMEG_0066	MSMEG_0055	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	Hypothetical protein; Identified by Glimmer2; putative.	0.899
esxA	groL	MSMEG_0066	MSMEG_0880	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	0.864
esxA	groL-2	MSMEG_0066	MSMEG_1583	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	0.580
groL	dnaK	MSMEG_0880	MSMEG_0709	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Chaperone protein DnaK; Acts as a chaperone; Belongs to the heat shock protein 70 family.	0.992
groL	esxA	MSMEG_0880	MSMEG_0066	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	0.864
groL	groL-2	MSMEG_0880	MSMEG_1583	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	0.982
groL	tuf	MSMEG_0880	MSMEG_1401	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Translation elongation factor Tu; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.	0.845
groL-2	dnaK	MSMEG_1583	MSMEG_0709	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Chaperone protein DnaK; Acts as a chaperone; Belongs to the heat shock protein 70 family.	0.991
groL-2	eccCb1	MSMEG_1583	MSMEG_0062	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Ftsk/spoiiie family protein; Part of the ESX-1 / type VII specialized secretion system (T7SS), which exports several proteins including EsxA and EsxB. Plays a role in DNA conjugation, in both donor and recipient strains.	0.416
groL-2	esxA	MSMEG_1583	MSMEG_0066	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Early secretory antigenic target, 6 kDa; An exported protein. Unlike its M.tuberculosis counterpart has poor pore forming ability in artificial liposomes, does not undergo conformational change at acidic pH. Mutation of 2 residues to those found in M.tuberculosis (25-TA-26 to IH) alters the properties of this protein so that it inserts into liposomes at acidic pH, forming pores, like its M.tuberculosis counterpart.	0.580
groL-2	groL	MSMEG_1583	MSMEG_0880	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.	0.982