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| | pafA | Proteasome component; Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction likely involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine. Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily. (452 aa) |
| | ecf | Extra cytoplasmic sigma factor; Identified by match to protein family HMM PF04542; match to protein family HMM TIGR02937; Belongs to the sigma-70 factor family. (391 aa) |
| | dnaK | Chaperone protein DnaK; Acts as a chaperone; Belongs to the heat shock protein 70 family. (622 aa) |
| | grpE | Co-chaperone GrpE; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-depend [...] (216 aa) |
| | dnaJ | Chaperone protein DnaJ; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, D [...] (390 aa) |
| | clpB | Chaperone ClpB; Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE; Belongs to the ClpA/ClpB family. (848 aa) |
| | groL | Chaperonin GroL; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. (541 aa) |
| | aceA | Isocitrate lyase; Identified by match to protein family HMM PF00463; match to protein family HMM TIGR01346. (428 aa) |
| | senX3 | Sensor histidine kinase SenX3; Part of the two-component regulatory system SenX3/RegX3. Phosphorylates RegX3 under conditions of phosphate limitation. Probably does not itself sense phosphate concentrations, which may be relayed to SenX3 by the PstSCAB phosphate transporter system. (384 aa) |
| | regX3 | DNA-binding response regulator RegX3; Part of the two-component regulatory system SenX3/RegX3. Once phosphorylated by SenX3, activates the expression of several operons/genes involved in phosphate assimilation and metabolism, such as pstSCAB, phnDCE, and phoA. (225 aa) |
| | ABK73512.1 | Phosphate/sulphate permease; Identified by match to protein family HMM PF01384. (418 aa) |
| | menG | Menaquinone biosynthesis methyltransferase UbiE; Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2). (229 aa) |
| | ABK73445.1 | Identified by match to protein family HMM PF00582. (293 aa) |
| | alr | Alanine racemase; Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids; Belongs to the alanine racemase family. (389 aa) |
| | whiB3 | Transcription factor WhiB; A redox-sensitive transcriptional regulator. Maintains intracellular redox homeostasis by regulating catabolic metabolism and polyketide biosynthesis. Regulates expression of the redox buffer ergothioneine (ERG). In concert with myothiol (MSH), another redox buffer, responds to low pH leading to acid resistance. The apo- but not holo-form probably binds DNA (By similarity). (96 aa) |
| | sigH | RNA polymerase sigma-70 factor, family protein; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released. This sigma factor is involved in heat shock and oxidative stress responses. (219 aa) |
| | pyc | Pyruvate carboxylase; Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. (1127 aa) |
| | ald | Alanine dehydrogenase; Catalyzes the reversible reductive amination of pyruvate to L-alanine. May be required for the adaptation from aerobic growth to anaerobic dormancy. I could be involved in the maintenance of the NAD pool during the shift to an anaerobic dormant state in which oxygen as a terminal electron acceptor becomes limited. (371 aa) |
| | ABK73518.1 | Polyphosphate glucokinase; Identified by match to protein family HMM PF00480. (272 aa) |
| | relA | GTP pyrophosphokinase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. (797 aa) |
| | ripA | Secreted cell wall-associated hydrolase; Peptidoglycan endopeptidase that cleaves the bond between D- glutamate and meso-diaminopimelate. Binds and degrades high-molecular weight peptidoglycan. Required for normal separation of daughter cells after cell division and for cell wall integrity (By similarity). (497 aa) |
| | bioA | Adenosylmethionine-8-amino-7-oxononanoate transaminase; Catalyzes the transfer of the alpha-amino group from S- adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily. (432 aa) |
| | bioF | 8-amino-7-oxononanoate synthase; Catalyzes the decarboxylative condensation of pimeloyl-[acyl- carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. (382 aa) |
| | bioD | Dethiobiotin synthase; Catalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8- diaminopelargonic acid (DAPA) to form an ureido ring. (225 aa) |
| | bioB | Biotin synthase; Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism; Belongs to the radical SAM superfamily. Biotin synthase family. (353 aa) |
| | ABK70785.1 | Universal stress protein family protein, putative; Identified by match to protein family HMM PF00582. (162 aa) |
| | katG | Catalase/peroxidase HPI; Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria. (748 aa) |
| | lon | ATP-dependent protease La; ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short- lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. (779 aa) |
| | aceA-2 | Isocitrate lyase; Identified by match to protein family HMM PF00463; match to protein family HMM TIGR01346. (769 aa) |
| | katG-2 | Catalase/peroxidase HPI; Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity; Belongs to the peroxidase family. Peroxidase/catalase subfamily. (688 aa) |
| | ABK75086.1 | Universal stress protein family protein, putative; Identified by match to protein family HMM PF00582. (147 aa) |
| | prcA | Proteasome alpha subunit; Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.smegmatis proteasome is able to cleave oligopeptides after hydrophobic residues, thus displaying chymotrypsin-like activity. In complex with the ATPase Mpa, degrades protein targets conjugated to a prokaryotic ubiquitin- like protein (Pup). Identified substrates of the M.smegmatis proteasome are the pupylated SodA and Ino1 proteins. The Pup- proteasome system (PPS) is essential for survival under starvation; PPS likely functions to recycle a [...] (246 aa) |
| | prcB | Proteasome beta subunit; Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.smegmatis proteasome is able to cleave oligopeptides after hydrophobic residues, thus displaying chymotrypsin-like activity. In complex with the ATPase Mpa, degrades protein targets conjugated to a prokaryotic ubiquitin- like protein (Pup). Among the identified substrates of the M.smegmatis proteasome are the pupylated SodA and Ino1 proteins. The Pup-proteasome system (PPS) is essential for survival under starvation; PPS likely functions to r [...] (280 aa) |
| | ABK72096.1 | Identified by match to protein family HMM PF00582. (263 aa) |
| | ABK71390.1 | Identified by match to protein family HMM PF00582. (293 aa) |
| | ABK69884.1 | Identified by match to protein family HMM PF00582. (273 aa) |
| | ABK73928.1 | Identified by match to protein family HMM PF00582; Belongs to the universal stress protein A family. (294 aa) |
| | ABK72172.1 | Identified by match to protein family HMM PF00582. (130 aa) |
| | ABK71201.1 | Identified by match to protein family HMM PF00877. (381 aa) |
| | ABK73898.1 | Identified by match to protein family HMM PF00582. (272 aa) |
| | dnaJ-2 | Chaperone protein DnaJ; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, D [...] (381 aa) |
| | nadD | Nicotinate (nicotinamide) nucleotide adenylyltransferase; Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD). (222 aa) |
| | nadE | Glutamine-dependent NAD+ synthetase; Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source. (680 aa) |
| | ABK76085.1 | Secreted protein. (433 aa) |
| | glgM | Glycogen synthase; Involved in the biosynthesis of the maltose-1-phosphate (M1P) building block required for alpha-glucan production by the key enzyme GlgE. Catalyzes the formation of an alpha-1,4 linkage between glucose from ADP-glucose and glucose 1-phosphate (G1P) to yield maltose-1- phosphate (M1P). (387 aa) |
| | ABK71731.1 | Identified by match to protein family HMM PF00582. (280 aa) |
| | rpfB | Resuscitation-promoting factor RpfB; Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity (By similarity). Belongs to the transglycosylase family. Rpf subfamily. (375 aa) |
| | ABK70087.1 | Identified by match to protein family HMM PF00582. (298 aa) |
| | ABK75323.1 | Identified by match to protein family HMM PF00582. (292 aa) |
| | kshA | Rieske [2Fe-2S] domain protein; Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost- 1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. (383 aa) |
| | kshB | Oxidoreductase, electron transfer component; Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost- 1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. (353 aa) |
| | kstR | Transcriptional regulator, TetR family protein; Controls the expression of genes used for utilizing diverse lipids as energy sources. (204 aa) |
| | carD | Transcriptional regulator, CarD family protein; Controls rRNA transcription by binding to the RNA polymerase (RNAP). Required for the stringent response to starvation, oxidative and genotoxic stresses as well as control of rRNA under normal growth conditions. (162 aa) |
| | katG-3 | Catalase/peroxidase HPI; Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria. (739 aa) |
| | treS | Trehalose synthase; Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation. Maltose is the preferred substrate. To a lesser extent, also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D- glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose. TreS plays a key role in the utilization of trehalose for the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P). Might also functio [...] (593 aa) |
| | pyc-2 | Pyruvate carboxylase; Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. (1131 aa) |
| | ponA1 | Penicillin-binding protein 1; Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross- linking of the peptide subunits) (By similarity). (785 aa) |
