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| | dapB | Dihydrodipicolinate reductase; Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate. (278 aa) |
| | tpiA | Triosephosphate isomerase; Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P); Belongs to the triosephosphate isomerase family. (269 aa) |
| | Psyc_0085 | Fructose-bisphosphate aldolase. (296 aa) |
| | argH | Argininosuccinate lyase. (457 aa) |
| | gltA | Citrate synthase; Belongs to the citrate synthase family. (426 aa) |
| | aroE | Shikimate dehydrogenase; Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA). (288 aa) |
| | dapA | Dihydrodipicolinate synthase; Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). (301 aa) |
| | proC | Pyrroline-5-carboxylate reductase; Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. (277 aa) |
| | prsA | Putative ribose-phosphate pyrophosphokinase; Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P); Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily. (315 aa) |
| | lysA | Diaminopimelate decarboxylase; Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine. (443 aa) |
| | dapF | Diaminopimelate epimerase; Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan. (295 aa) |
| | hom | Homoserine dehydrogenase; Monofunctional enzyme (hom). Contains no aspartokinase domain. (448 aa) |
| | icd2 | Isocitrate dehydrogenase; Belongs to the monomeric-type IDH family. (739 aa) |
| | glyA | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (418 aa) |
| | proA | Glutamate-5-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. Belongs to the gamma-glutamyl phosphate reductase family. (433 aa) |
| | cysM | Cysteine Synthase B (CysM) can use thiosulfate to produce cysteine thiosulfonate in addition to use of hydrogen sulfide; Belongs to the cysteine synthase/cystathionine beta- synthase family. (318 aa) |
| | serA | D-3-phosphoglycerate dehydrogenase; ACT feedback inhibition domain is present; Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. (408 aa) |
| | metX | Homoserine O-acetyltransferase; Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. (445 aa) |
| | Psyc_0393 | Succinyldiaminopimelate aminotransferase apoenzyme; Class not determined. (408 aa) |
| | metH | Methionine synthase (B12-dependent); Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. (1271 aa) |
| | trpF | Phosphoribosylanthranilate isomerase; Belongs to the TrpF family. (227 aa) |
| | trpB | Tryptophan synthase, beta chain; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. (424 aa) |
| | trpA | Tryptophan synthase, alpha chain; The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. Belongs to the TrpA family. (278 aa) |
| | ilvB | Acetolactate synthase, large subunit. (608 aa) |
| | ilvH | Acetolactate synthase, small subunit; ACT domain is present in this orf. (165 aa) |
| | ilvC | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. (338 aa) |
| | dapE | Succinyldiaminopimelate desuccinylase; Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls; Belongs to the peptidase M20A family. DapE subfamily. (407 aa) |
| | argB | N-acetylglutamate kinase; Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate; Belongs to the acetylglutamate kinase family. ArgB subfamily. (303 aa) |
| | asnB | Probable asparagine synthase, glutamine-hydrolyzing; There are two asparagine synthase genes present in the Psychrobacter 273-4 genome. (623 aa) |
| | wbpS | Probable asparagine synthase, glutamine-hydrolyzing; There are two asparagine synthases in the Psychrobacter 273-4 genome. (651 aa) |
| | hisZ | Possible ATP phosphoribosyltransferase regulatory subunit; Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine. (434 aa) |
| | pgm | Phosphoglycerate mutase; Catalyzes the interconversion of 2-phosphoglycerate and 3- phosphoglycerate. (552 aa) |
| | pgk | Phosphoglycerate kinase; Based on BlastP, PFAM and COGS alignments; Belongs to the phosphoglycerate kinase family. (400 aa) |
| | fba | Fructose-bisphosphate aldolase; Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. (345 aa) |
| | Psyc_0717 | Aminotransferase; Specific substrate unknown. Contains a moderately strong hit to a helix-turn-helix motif. (546 aa) |
| | dapD | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase; Belongs to the transferase hexapeptide repeat family. (273 aa) |
| | acnB | Aconitase; Belongs to the aconitase/IPM isomerase family. (867 aa) |
| | metK | Methionine adenosyltransferase; Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. (388 aa) |
| | metE | Methionine synthase (B12-independent). (345 aa) |
| | aroF | 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase; Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino- heptulosonate-7-phosphate (DAHP). (360 aa) |
| | aroQ | 3-dehydroquinate dehydratase; Catalyzes a trans-dehydration via an enolate intermediate. Belongs to the type-II 3-dehydroquinase family. (178 aa) |
| | ilvA | L-threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. (517 aa) |
| | rpiA | Ribose-5-phosphate isomerase; Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate. (219 aa) |
| | argF | Ornithine carbamoyltransferase; Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. (307 aa) |
| | trpC | Indole-3-glycerol phosphate synthase; Located adjacent to trpG and trpD previously described, In fungi, IGPS is the central domain of a trifunctional enzyme that contains a PRAI C-terminal domain and a glutamine amidotransferase at N- terminus InterPro:(); Belongs to the TrpC family. (295 aa) |
| | trpD | Anthranilate phosphoribosyltransferase; Catalyzes the transfer of the phosphoribosyl group of 5- phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'- phosphoribosyl)-anthranilate (PRA). (379 aa) |
| | glnA | L-glutamine synthetase; Contains adenylation site. (469 aa) |
| | serC | Phosphoserine aminotransferase apoenzyme; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. (373 aa) |
| | argD | Acetylornithine aminotransferase apoenzyme; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily. (393 aa) |
| | cysE | Serine O-acetyltransferase; Contains four hexapeptide repeat motifs. (303 aa) |
| | aroA | 3-phosphoshikimate 1-carboxyvinyltransferase / prephenate dehydrogenase; Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. (778 aa) |
| | hisC | Aminotransferase; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily. (380 aa) |
| | pheA | Prephenate dehydratase; Contains 3 domains: chorismate mutase, prephenate dehydratase, and an ACT (amino acid synthesis regulatory domain). This enzyme is bifunctional in the phenylalanine synthesis pathway. (393 aa) |
| | tal | Transaldolase; Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway; Belongs to the transaldolase family. Type 1 subfamily. (313 aa) |
| | sdaA | L-serine ammonia-lyase; Beta chain domain at N-terminus followed closely by alpha chain to C-terminus; Belongs to the iron-sulfur dependent L-serine dehydratase family. (477 aa) |
| | argJ | Glutamate N-acetyltransferase; Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate. Belongs to the ArgJ family. (407 aa) |
| | ilvE | Branched chain amino acid aminotransferase apoenzyme; Acts on leucine, isoleucine and valine. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. (309 aa) |
| | leuA | Probable 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. (594 aa) |
| | ilvD | Dihydroxyacid dehydratase; Belongs to the IlvD/Edd family. (628 aa) |
| | tyrB | Aromatic amino acid aminotransferase apoenzyme. (398 aa) |
| | leuB | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. (367 aa) |
| | leuD | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. (216 aa) |
| | leuC | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (472 aa) |
| | aroC | Chorismate synthase; Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system. (367 aa) |
| | lysC | Aspartate kinase; Monofunctional enzyme, there is no fused homoserine dehydydrogenase domain. Contains two ACT domains; Belongs to the aspartokinase family. (428 aa) |
| | argC | N-acetyl-gamma-glutamyl-phosphate reductase; Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. (355 aa) |
| | proB | Glutamate 5-kinase; Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate. (390 aa) |
| | gapA | d-Glyceraldehyde 3-phosphate dehydrogenase; Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. (481 aa) |
| | asd | Aspartate semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. (377 aa) |
| | gpdh | Probable Glyceraldehyde 3-phosphate dehydrogenase family; gapA domain; Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. (370 aa) |
| | tktA | Transketolase; Belongs to the transketolase family. (665 aa) |
| | argG | Argininosuccinate synthase; Citrulline-aspartate ligase; Belongs to the argininosuccinate synthase family. Type 1 subfamily. (412 aa) |
| | eno | Enolase; Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. (438 aa) |
| | trpE | Anthranilate synthase, component I. (472 aa) |
| | Psyc_1668 | Anthranilate synthase, component II. (215 aa) |
| | rpe | Ribulose-5-phosphate 3-epimerase; Belongs to the ribulose-phosphate 3-epimerase family. (229 aa) |
| | argE | Acetylornithine deacetylase. (412 aa) |
| | serB | Phosphoserine phosphatase; This orf contains no alignments to the ACT domain. (400 aa) |
| | gltD | Glutamate synthase (NADPH) small subunit. (473 aa) |
| | gltB | Glutamate synthase (NADPH) large subunit; This orf aligns best with NADPH-dependent glutamate synthase proteins as opposed to proteins that use other cofactors. (1487 aa) |
| | aroB | 3-dehydroquinate synthase; Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ); Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family. (388 aa) |
| | aroK | Shikimate kinase; Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate; Belongs to the shikimate kinase family. (186 aa) |
| | hisC2 | Histidinol phosphate aminotransferase apoenzyme; One of two hisC genes in the Psychrobacter 273-4 genome; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily. (377 aa) |
| | hisD | Histidinol dehydrogenase; Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine. (431 aa) |
| | hisG | ATP phosphoribosyltransferase (homohexameric); Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity. Belongs to the ATP phosphoribosyltransferase family. Short subfamily. (231 aa) |
| | hisI | phosphoribosyl-AMP cyclohydrolase; This orf aligns to domains of both pyrophosphohydrolase and cyclohydrolase functions, suggesting a bifunctional enzyme; In the N-terminal section; belongs to the PRA-CH family. (282 aa) |
| | acnA | Aconitase; Catalyzes the isomerization of citrate to isocitrate via cis- aconitate. (939 aa) |
| | thrB | Homoserine kinase; Belongs to the pseudomonas-type ThrB family. (391 aa) |
| | hisF | Imidazole glycerol phosphate synthase subunit hisF; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit. (260 aa) |
| | argA | N-acetylglutamate synthase; Belongs to the acetyltransferase family. ArgA subfamily. (440 aa) |
| | hisA | 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase. (246 aa) |
| | hisH | Imidazole glycerol phosphate synthase subunit hisH; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF. (214 aa) |
| | hisB | Imidazoleglycerol-phosphate dehydratase; This orf is probably the monofunctional variety of hisB. (221 aa) |
| | thrC | L-threonine synthase. (473 aa) |
