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| | aroQ | 3-dehydroquinate dehydratase; Catalyzes a trans-dehydration via an enolate intermediate. Belongs to the type-II 3-dehydroquinase family. (152 aa) |
| | Mfla_0031 | Oxidoreductase FAD/NAD(P)-binding protein. (342 aa) |
| | argH | TIGRFAM: argininosuccinate lyase; PFAM: fumarate lyase; KEGG: tbd:Tbd_0229 argininosuccinate lyase; COGs: COG0165 Argininosuccinate lyase category=E. (468 aa) |
| | gmk | Guanylate kinase; Essential for recycling GMP and indirectly, cGMP. (202 aa) |
| | argC | N-acetyl-gamma-glutamyl-phosphate reductase; Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. (350 aa) |
| | folD | 5,10-methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase; Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. (289 aa) |
| | ilvA | L-threonine ammonia-lyase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. (503 aa) |
| | Mfla_0191 | 5,10-methylenetetrahydrofolate reductase; Belongs to the methylenetetrahydrofolate reductase family. (280 aa) |
| | ahcY | Adenosylhomocysteinase; May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine. (470 aa) |
| | metK | Methionine adenosyltransferase; Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. (387 aa) |
| | dapF | Diaminopimelate epimerase; Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan. (290 aa) |
| | Mfla_0204 | Regulatory inactivation of DnaA Hda protein; Belongs to the DnaA family. (205 aa) |
| | purM | TIGRFAM: phosphoribosylformylglycinamidine cyclo-ligase; PRIAM: Phosphoribosylformylglycinamidine cyclo-ligase; PFAM: AIR synthase related protein; AIR synthase related protein domain protein; KEGG: rso:RSc2623 phosphoribosylaminoimidazole synthetase; COGs: COG0150 Phosphoribosylaminoimidazole (AIR) synthetase category=F. (346 aa) |
| | hisG | ATP phosphoribosyltransferase (homohexameric); Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity. Belongs to the ATP phosphoribosyltransferase family. Short subfamily. (214 aa) |
| | hisD | Histidinol dehydrogenase; Catalyzes the sequential NAD-dependent oxidations of L- histidinol to L-histidinaldehyde and then to L-histidine. (432 aa) |
| | hisC | TIGRFAM: histidinol-phosphate aminotransferase; PFAM: aminotransferase, class I and II; KEGG: tbd:Tbd_1712 histidinol-phosphate aminotransferase; COGs: COG0079 Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase category=E; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily. (354 aa) |
| | hisB | PRIAM: Imidazoleglycerol-phosphate dehydratase; PFAM: imidazoleglycerol-phosphate dehydratase; KEGG: tbd:Tbd_1711 imidazoleglycerol-phosphate dehydratase; COGs: COG0131 Imidazoleglycerol-phosphate dehydratase category=E. (198 aa) |
| | hisH | Imidazole glycerol phosphate synthase subunit hisH; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF. (214 aa) |
| | hisA | 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; TIGRFAM: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; PFAM: histidine biosynthesis; KEGG: tbd:Tbd_1709 phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; COGs: COG0106 Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase category=E. (247 aa) |
| | hisF | Imidazole glycerol phosphate synthase subunit hisF; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit. (252 aa) |
| | hisI | phosphoribosyl-AMP cyclohydrolase; Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP. (130 aa) |
| | hisE | PFAM: phosphoribosyl-ATP pyrophosphohydrolase; KEGG: tbd:Tbd_1706 phosphoribosyl-ATP pyrophosphohydrolase; COGs: COG0140 Phosphoribosyl-ATP pyrophosphohydrolase category=E. (106 aa) |
| | Mfla_0257 | Histidine triad (HIT) protein. (114 aa) |
| | dut | Deoxyuridine 5'-triphosphate nucleotidohydrolase; This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA; Belongs to the dUTPase family. (150 aa) |
| | Mfla_0314 | Phosphopantothenate-cysteine ligase / Phosphopantothenoylcysteine decarboxylase; Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine; In the C-terminal section; belongs to the PPC synthetase family. (411 aa) |
| | ilvD | TIGRFAM: dihydroxy-acid dehydratase; PRIAM: Dihydroxy-acid dehydratase; PFAM: dihydroxy-acid and 6-phosphogluconate dehydratase; KEGG: cvi:CV1277 dihydroxy-acid dehydratase; COGs: COG0129 Dihydroxyacid dehydratase/phosphogluconate dehydratase category=G; Belongs to the IlvD/Edd family. (616 aa) |
| | purD | TIGRFAM: phosphoribosylamine--glycine ligase; PRIAM: Phosphoribosylamine--glycine ligase; PFAM: phosphoribosylglycinamide synthetase; ATP-dependent carboxylate-amine ligase domain protein, ATP-grasp; KEGG: dar:Daro_3668 phosphoribosylglycinamide synthetase; COGs: COG0151 Phosphoribosylamine-glycine ligase category=F; Belongs to the GARS family. (422 aa) |
| | purH | TIGRFAM: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; PRIAM: Phosphoribosylaminoimidazolecarboxamide formyltransferase; PFAM: AICARFT/IMPCHase bienzyme; MGS domain protein; KEGG: bma:BMA2356 phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; COGs: COG0138 AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful) category=F. (528 aa) |
| | Mfla_0471 | PfkB. (320 aa) |
| | ribH | 6,7-dimethyl-8-ribityllumazine synthase; Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. (149 aa) |
| | cysS | cysteinyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (455 aa) |
| | pyrD | Dihydroorotate oxidase A; Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor; Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. (337 aa) |
| | leuA | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily. (515 aa) |
| | Mfla_0520 | HAD-superfamily subfamily IB, PSPase-like protein. (220 aa) |
| | Mfla_0532 | Conserved hypothetical protein. (294 aa) |
| | Mfla_0567 | TIGRFAM: aspartate kinase; aspartate kinase, monofunctional class; PRIAM: Aspartate kinase; PFAM: aspartate/glutamate/uridylate kinase; amino acid-binding ACT domain protein; KEGG: neu:NE2132 aspartate kinase; COGs: COG0527 Aspartokinases category=E; Belongs to the aspartokinase family. (408 aa) |
| | Mfla_0599 | 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase. (163 aa) |
| | Mfla_0615 | PRIAM: Serine O-acetyltransferase; KEGG: neu:NE0575 bacterial transferase hexapeptide repeat; COGs: COG1045 Serine acetyltransferase category=E. (317 aa) |
| | ilvE | Branched chain amino acid aminotransferase apoenzyme; Acts on leucine, isoleucine and valine. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. (306 aa) |
| | Mfla_0724 | D-3-phosphoglycerate dehydrogenase; Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. (410 aa) |
| | leuA-2 | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. (562 aa) |
| | nadK | NAD(+) kinase; Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. (290 aa) |
| | dapB | Dihydrodipicolinate reductase; Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate; Belongs to the DapB family. (267 aa) |
| | carA | TIGRFAM: carbamoyl-phosphate synthase, small subunit; PFAM: glutamine amidotransferase class-I; Carbamoyl-phosphate synthase, small chain; KEGG: tbd:Tbd_1127 carbamoyl-phosphate synthase, small subunit; COGs: COG0505 Carbamoylphosphate synthase small subunit category=F; Belongs to the CarA family. (376 aa) |
| | carB | TIGRFAM: carbamoyl-phosphate synthase, large subunit; PFAM: Carbamoyl-phosphate synthase L chain, ATP-binding; Carbamoyl-phosphate synthetase large chain, oligomerisation; Carbamoyl-phosphate synthetase large chain domain protein; MGS domain protein; KEGG: tbd:Tbd_1128 carbamoyl-phosphate synthase, large subunit, glutamine-dependent; COGs: COG0458 Carbamoylphosphate synthase large subunit (split gene in MJ) category=F; Belongs to the CarB family. (1068 aa) |
| | Mfla_0784 | Dihydropteroate synthase; Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8- dihydropteroate (H2Pte), the immediate precursor of folate derivatives. (279 aa) |
| | Mfla_0811 | TIGRFAM: serine O-acetyltransferase; PRIAM: Serine O-acetyltransferase; PFAM: transferase hexapeptide repeat containing protein; KEGG: tbd:Tbd_1162 serine O-acetyltransferase; COGs: COG1045 Serine acetyltransferase category=E. (250 aa) |
| | dapA | Dihydrodipicolinate synthase; Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). (290 aa) |
| | Mfla_0855 | L-aspartate oxidase; Catalyzes the oxidation of L-aspartate to iminoaspartate. (538 aa) |
| | purL | Phosphoribosylformylglycinamidine synthase; Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. (1297 aa) |
| | Mfla_0903 | Aminotransferase. (429 aa) |
| | Mfla_0904 | PRIAM: Homoserine dehydrogenase; PFAM: homoserine dehydrogenase; amino acid-binding ACT domain protein; homoserine dehydrogenase, NAD-binding; KEGG: eba:ebA4952 homoserine dehydrogenase; COGs: COG0460 Homoserine dehydrogenase category=E. (437 aa) |
| | Mfla_0905 | TIGRFAM: threonine synthase; PRIAM: Threonine synthase; PFAM: Pyridoxal-5'-phosphate-dependent enzyme, beta subunit; KEGG: dar:Daro_2382 threonine synthase; COGs: COG0498 Threonine synthase category=E. (475 aa) |
| | dcd | Deoxycytidine triphosphate deaminase; Catalyzes the deamination of dCTP to dUTP. (188 aa) |
| | pyrF | Orotidine 5'-phosphate decarboxylase; Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP); Belongs to the OMP decarboxylase family. Type 1 subfamily. (232 aa) |
| | aroA | 3-phosphoshikimate 1-carboxyvinyltransferase; Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. (429 aa) |
| | Mfla_0930 | PRIAM: Prephenate dehydrogenase; PFAM: Prephenate dehydrogenase; 6-phosphogluconate dehydrogenase, NAD-binding; NAD-dependent glycerol-3-phosphate dehydrogenase domain protein; Ketopantoate reductase ApbA/PanE, N-terminal domain protein; KEGG: dar:Daro_1234 prephenate dehydrogenase:ketopantoate reductase ApbA/PanE:NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal; COGs: COG0287 Prephenate dehydrogenase category=E. (293 aa) |
| | Mfla_0999 | TIGRFAM: L-aspartate oxidase; PRIAM: L-aspartate oxidase; PFAM: fumarate reductase/succinate dehydrogenase flavoprotein domain protein; KEGG: eba:ebA5461 L-aspartate oxidase; COGs: COG0029 Aspartate oxidase category=H. (538 aa) |
| | Mfla_1031 | TIGRFAM: phosphoribosylformylglycinamidine synthase; PRIAM: Phosphoribosylformylglycinamidine synthase; PFAM: AIR synthase related protein; AIR synthase related protein domain protein; KEGG: purL; phosphoribosylformylglycinamidine synthase; COGs: COG0046 Phosphoribosylformylglycinamidine (FGAM) synthase synthetase domain category=F. (1297 aa) |
| | Mfla_1073 | TIGRFAM: 3-phosphoshikimate 1-carboxyvinyltransferase; PRIAM: 3-phosphoshikimate 1-carboxyvinyltransferase; PFAM: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase); KEGG: cvi:CV3048 3-phosphoshikimate 1-carboxyvinyltransferase; COGs: COG0128 5-enolpyruvylshikimate-3-phosphate synthase category=E. (429 aa) |
| | Mfla_1074 | PRIAM: Prephenate dehydrogenase; PFAM: Prephenate dehydrogenase; 6-phosphogluconate dehydrogenase, NAD-binding; NAD-dependent glycerol-3-phosphate dehydrogenase domain protein; Ketopantoate reductase ApbA/PanE, N-terminal domain protein; KEGG: dar:Daro_1234 prephenate dehydrogenase:ketopantoate reductase ApbA/PanE:NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal; COGs: COG0287 Prephenate dehydrogenase category=E. (293 aa) |
| | guaA | GMP synthase (glutamine-hydrolyzing); Catalyzes the synthesis of GMP from XMP. (526 aa) |
| | guaB | Inosine-5'-monophosphate dehydrogenase; Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Belongs to the IMPDH/GMPR family. (486 aa) |
| | purT | Formate-dependent phosphoribosylglycinamide formyltransferase; Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate; Belongs to the PurK/PurT family. (400 aa) |
| | Mfla_1280 | Phosphoribosyltransferase. (185 aa) |
| | Mfla_1319 | Aminotransferase. (398 aa) |
| | ribA | GTP cyclohydrolase II; Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate; Belongs to the GTP cyclohydrolase II family. (209 aa) |
| | Mfla_1386 | Succinyldiaminopimelate aminotransferase apoenzyme. (396 aa) |
| | purA | Adenylosuccinate synthetase; Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP; Belongs to the adenylosuccinate synthetase family. (440 aa) |
| | hisZ | tRNA synthetase, class II (G, H, P and S); Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine. (387 aa) |
| | ndk | Nucleoside diphosphate kinase; Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate; Belongs to the NDK family. (141 aa) |
| | Mfla_1648 | Aminodeoxychorismate synthase, subunit I. (460 aa) |
| | hisC-2 | Aminotransferase; TIGRFAM: histidinol-phosphate aminotransferase; PFAM: aminotransferase, class I and II; KEGG: tbd:Tbd_0952 histidinol-phosphate aminotransferase; COGs: COG0079 Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase category=E; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily. (369 aa) |
| | Mfla_1686 | TIGRFAM: chorismate mutase; PFAM: prephenate dehydratase; Chorismate mutase; amino acid-binding ACT domain protein; KEGG: cvi:CV2355 chorismate mutase/prephenate dehydratase; COGs: COG0077 Prephenate dehydratase category=E. (355 aa) |
| | serC | Phosphoserine aminotransferase apoenzyme; Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. (360 aa) |
| | metZ | O-succinylhomoserine sulfhydrylase; Catalyzes the formation of L-homocysteine from O-succinyl-L- homoserine (OSHS) and hydrogen sulfide. (392 aa) |
| | purF | Amidophosphoribosyltransferase; Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine; In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. (511 aa) |
| | Mfla_1695 | FolC bifunctional protein; Belongs to the folylpolyglutamate synthase family. (432 aa) |
| | trpA | Tryptophan synthase, alpha chain; The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. Belongs to the TrpA family. (268 aa) |
| | trpB | Tryptophan synthase, beta chain; The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine. (400 aa) |
| | trpF | PRIAM: Phosphoribosylanthranilate isomerase; PFAM: N-(5'phosphoribosyl)anthranilate isomerase (PRAI); KEGG: noc:Noc_1019 phosphoribosylanthranilate isomerase; COGs: COG0135 Phosphoribosylanthranilate isomerase category=E; Belongs to the TrpF family. (192 aa) |
| | asd | Aspartate semialdehyde dehydrogenase; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family. (370 aa) |
| | leuB | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. (351 aa) |
| | leuD | 3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily. (214 aa) |
| | leuC | 3-isopropylmalate dehydratase, large subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. (468 aa) |
| | ppnP | Protein of unknown function DUF1255; Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases. Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. Also catalyzes the reverse reactions. (103 aa) |
| | argG | PRIAM: Argininosuccinate synthase; PFAM: argininosuccinate synthase; ExsB family protein; KEGG: eba:ebA5097 argininosuccinate synthase; COGs: COG0137 Argininosuccinate synthase category=E; Belongs to the argininosuccinate synthase family. Type 1 subfamily. (406 aa) |
| | argF | Ornithine carbamoyltransferase; Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. (306 aa) |
| | argD | TIGRFAM: acetylornithine and succinylornithine aminotransferases; PFAM: aminotransferase class-III; KEGG: tbd:Tbd_1851 acetylornithine and succinylornithine aminotransferase; COGs: COG4992 Ornithine/acetylornithine aminotransferase category=E; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily. (392 aa) |
| | Mfla_1819 | TIGRFAM: adenylosuccinate lyase; PRIAM: Adenylosuccinate lyase; PFAM: fumarate lyase; KEGG: tbd:Tbd_0518 adenylosuccinate lyase; COGs: COG0015 Adenylosuccinate lyase category=F; Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily. (456 aa) |
| | Mfla_1861 | TIGRFAM: cysteine synthases; cysteine synthase B; PFAM: Pyridoxal-5'-phosphate-dependent enzyme, beta subunit; KEGG: dar:Daro_1287 cysteine synthase K/M:cysteine synthase B; COGs: COG0031 Cysteine synthase category=E; Belongs to the cysteine synthase/cystathionine beta- synthase family. (295 aa) |
| | dapD | TIGRFAM: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase; PRIAM: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase; PFAM: transferase hexapeptide repeat containing protein; KEGG: tbd:Tbd_1220 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase; COGs: COG2171 Tetrahydrodipicolinate N-succinyltransferase category=E; Belongs to the transferase hexapeptide repeat family. (274 aa) |
| | nadE | NH(3)-dependent NAD(+) synthetase; Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source. (535 aa) |
| | Mfla_1890 | TIGRFAM: phosphoserine phosphatase SerB; HAD-superfamily hydrolase, subfamily IB (PSPase-like); PRIAM: Phosphoserine phosphatase; PFAM: Haloacid dehalogenase domain protein hydrolase; Haloacid dehalogenase domain protein hydrolase, type 3; KEGG: neu:NE0439 phosphoserine phosphatase protein; COGs: COG0560 Phosphoserine phosphatase category=E. (302 aa) |
| | Mfla_1891 | Peptidyl-prolyl cis-trans isomerase, cyclophilin type; PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides; Belongs to the cyclophilin-type PPIase family. (183 aa) |
| | pyrG | CTP synthase; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates. (542 aa) |
| | Mfla_1915 | Diaminohydroxyphosphoribosylaminopyrimidine deaminase; Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'- phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'- phosphate; In the C-terminal section; belongs to the HTP reductase family. (377 aa) |
| | glyA | Serine hydroxymethyltransferase; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. (430 aa) |
| | Mfla_2097 | Riboflavin synthase, alpha subunit. (199 aa) |
| | ribB | 3,4-dihydroxy-2-butanone 4-phosphate synthase / GTP cyclohydrolase II; Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate; Belongs to the DHBP synthase family. (361 aa) |
| | Mfla_2101 | Phosphoribosyltransferase. (174 aa) |
| | pyrB | TIGRFAM: aspartate carbamoyltransferase; PFAM: aspartate/ornithine carbamoyltransferase, Asp/Orn-binding region; aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain; KEGG: tbd:Tbd_2582 aspartate carbamoyltransferase; COGs: COG0540 Aspartate carbamoyltransferase catalytic chain category=F; Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family. (317 aa) |
| | Mfla_2103 | TIGRFAM: dihydroorotase, multifunctional complex type; PFAM: amidohydrolase; KEGG: neu:NE1664 dihydroorotase; COGs: COG0044 Dihydroorotase and related cyclic amidohydrolases category=F. (424 aa) |
| | Mfla_2108 | Protein of unknown function UPF0001; Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis; Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family. (241 aa) |
| | proC | Pyrroline-5-carboxylate reductase; Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. (269 aa) |
| | Mfla_2110 | Protein of unknown function YGGT. (191 aa) |
| | Mfla_2117 | TIGRFAM: nicotinate-nucleotide pyrophosphorylase; PRIAM: Nicotinate-nucleotide diphosphorylase (carboxylating); PFAM: Quinolinate phosphoribosyl transferase; thiamine monophosphate synthase; KEGG: bma:BMA2235 nicotinate-nucleotide pyrophosphorylase; COGs: COG0157 Nicotinate-nucleotide pyrophosphorylase category=H; Belongs to the NadC/ModD family. (289 aa) |
| | Mfla_2119 | TIGRFAM: acetolactate synthase, large subunit, biosynthetic type; PFAM: thiamine pyrophosphate enzyme domain protein TPP-binding; thiamine pyrophosphate enzyme, central region; thiamine pyrophosphate enzyme TPP binding domain protein; KEGG: tbd:Tbd_1991 acetolactate synthase, large subunit; COGs: COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase pyruvate dehydrogenase (cytochrome) glyoxylate carboligase phosphonopyruvate decarboxylase] category=H. (568 aa) |
| | Mfla_2120 | TIGRFAM: acetolactate synthase, small subunit; PFAM: amino acid-binding ACT domain protein; KEGG: tbd:Tbd_1990 acetolactate synthase, small subunit; COGs: COG0440 Acetolactate synthase small (regulatory) subunit category=E. (163 aa) |
| | ilvC | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. (338 aa) |
| | aroC | Chorismate synthase; Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system. (361 aa) |
| | adk | Adenylate kinase; Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism; Belongs to the adenylate kinase family. (218 aa) |
| | proA | Glutamate-5-semialdehyde dehydrogenase; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. Belongs to the gamma-glutamyl phosphate reductase family. (419 aa) |
| | nadD | Nicotinate-nucleotide adenylyltransferase; Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD). (237 aa) |
| | purK | 5-(carboxyamino)imidazole ribonucleotide synthase; Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR). (380 aa) |
| | purE | 5-(carboxyamino)imidazole ribonucleotide mutase; Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR). (164 aa) |
| | proB | Glutamate 5-kinase; Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate. (372 aa) |
| | obg | Small GTP-binding protein domain; An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control. Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. (353 aa) |
| | argJ | Glutamate N-acetyltransferase; Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate. Belongs to the ArgJ family. (410 aa) |
| | purC | TIGRFAM: phosphoribosylaminoimidazole-succinocarboxamide synthase; PRIAM: Phosphoribosylaminoimidazole-succinocarboxamide synthase; PFAM: SAICAR synthetase; KEGG: tbd:Tbd_2668 phosphoribosylaminoimidazole-succinocarboxamide (SAICAR) synthetase; COGs: COG0152 Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase category=F. (297 aa) |
| | argA | N-acetylglutamate synthase / N-acetylglutamate kinase; TIGRFAM: amino-acid N-acetyltransferase; PFAM: GCN5-related N-acetyltransferase; aspartate/glutamate/uridylate kinase; helix-turn-helix domain protein; KEGG: tbd:Tbd_0154 amino-acid N-acetyltransferase (ArgA); COGs: COG0548 Acetylglutamate kinase category=E; Belongs to the acetyltransferase family. ArgA subfamily. (521 aa) |
| | fhs | PRIAM: Formate--tetrahydrofolate ligase; PFAM: formate-tetrahydrofolate ligase, FTHFS; KEGG: mca:MCA2219 formate--tetrahydrofolate ligase; COGs: COG2759 Formyltetrahydrofolate synthetase category=F. (557 aa) |
| | argB | N-acetylglutamate kinase; Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate; Belongs to the acetylglutamate kinase family. ArgB subfamily. (294 aa) |
| | Mfla_2328 | Dihydroneopterin aldolase; Catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8-dihydropterin. (124 aa) |
| | nadA | Quinolinate synthetase A; Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate. (379 aa) |
| | apt | Adenine phosphoribosyltransferase; Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. (176 aa) |
| | lysA | Diaminopimelate decarboxylase; Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine. (445 aa) |
| | aroE | Shikimate dehydrogenase; Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA). (270 aa) |
| | aroB | 3-dehydroquinate synthase; Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ). (361 aa) |
| | aroK | Shikimate kinase; Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate; Belongs to the shikimate kinase family. (170 aa) |
| | trpC | PRIAM: Indole-3-glycerol-phosphate synthase; PFAM: Indole-3-glycerol phosphate synthase; KEGG: dar:Daro_3475 indole-3-glycerol phosphate synthase; COGs: COG0134 Indole-3-glycerol phosphate synthase category=E; Belongs to the TrpC family. (271 aa) |
| | trpD | Anthranilate phosphoribosyltransferase; Catalyzes the transfer of the phosphoribosyl group of 5- phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'- phosphoribosyl)-anthranilate (PRA). (341 aa) |
| | Mfla_2468 | Anthranilate synthase, component II; TIGRFAM: glutamine amidotransferase of anthranilate synthase; PFAM: glutamine amidotransferase class-I; KEGG: tbd:Tbd_2224 glutamine amidotransferase of anthranilate synthase; COGs: COG0512 Anthranilate/para-aminobenzoate synthases component II category=H. (188 aa) |
| | trpE | Anthranilate synthase, component I; Part of a heterotetrameric complex that catalyzes the two- step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concent [...] (494 aa) |
| | pyrE | Orotate phosphoribosyltransferase; Catalyzes the transfer of a ribosyl phosphate group from 5- phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP). (196 aa) |
| | Mfla_2509 | Methionine synthase (B12-dependent); Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. (1262 aa) |
| | metE | Methionine synthase (B12-independent); Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation; Belongs to the vitamin-B12 independent methionine synthase family. (763 aa) |
