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| | MYPU_0090 | 50S RIBOSOMAL PROTEIN L11; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. (217 aa) |
| | MYPU_0100 | 50S RIBOSOMAL PROTEIN L1; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (229 aa) |
| | MYPU_0230 | NADH OXIDASE (NOXASE); Identified by sequence similarity; probable; similar to SWISS-PROT:P75389 (NAOX_MYCPN) Blastp2 P=0 C=62%. (478 aa) |
| | MYPU_0680 | Conserved hypothetical protein; Identified by sequence similarity; similar to trEMBL:AAF09169 Blastp2 P=2e-55 score=215 Pfscan: pos.13-202 PF01145 | BAND_7 Band 7 family (PDOC00977). (309 aa) |
| | MYPU_0690 | ATP-DEPENDENT RNA HELICASE; Identified by sequence similarity; possible; similar to SWISS-PROT:P23304 (DEAD_ECOLI) Blastp2 P=2e-67 C=42%. (480 aa) |
| | MYPU_0930 | LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE); Identified by sequence similarity; probable; similar to SWISS-PROT:P36430 (SYL_BACSU) Blastp2 P=0 C=70%; Belongs to the class-I aminoacyl-tRNA synthetase family. (807 aa) |
| | MYPU_1040 | PREPROTEIN TRANSLOCASE SECA SUBUNIT; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane. (867 aa) |
| | MYPU_1050 | PEPTIDE CHAIN RELEASE FACTOR 1 (RF-1); Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. (359 aa) |
| | MYPU_1060 | PROTOPORPHIROGEN OXIDASE HEMK; Identified by sequence similarity; possible; similar to SWISS-PROT:P45873 (HEMK_BACSU) Blastp2 P=1e-16 C=48%. (230 aa) |
| | MYPU_1400 | 50S RIBOSOMAL PROTEIN L31; Binds the 23S rRNA; Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily. (69 aa) |
| | MYPU_1420 | HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR; Negative regulator of class I heat shock genes (grpE-dnaK- dnaJ and groELS operons). Prevents heat-shock induction of these operons. (347 aa) |
| | MYPU_1430 | HEAT SHOCK PROTEIN GRPE (activation of DNAK); Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. S [...] (298 aa) |
| | MYPU_1520 | Conserved hypothetical protein; Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins (By similarity). (608 aa) |
| | MYPU_1540 | 50S RIBOSOMAL PROTEIN L34; Identified by sequence similarity; probable; similar to SWISS-PROT:P45647 (RL34_COXBU) Blastp2 P=2e-13 C=76%; Belongs to the bacterial ribosomal protein bL34 family. (48 aa) |
| | MYPU_1780 | TRNA/RRNA METHYLTRANSFERASE; Identified by sequence similarity; possible; similar to SWISS-PROT:Q06753 (YACO_BACSU) Blastp2 P=7e-33 C=56%; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (229 aa) |
| | MYPU_1800 | RIBOSOMAL PROTEIN L33; Identified by sequence similarity; similar to SWISSPROT:P35870 (RL33_BACLI) Blast2p P=3e-4 score=39.5; Belongs to the bacterial ribosomal protein bL33 family. (46 aa) |
| | MYPU_1810 | Unknown; Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. (89 aa) |
| | MYPU_1820 | TRANSCRIPTION ANTITERMINATION PROTEIN; Participates in transcription elongation, termination and antitermination. (196 aa) |
| | MYPU_1830 | PROLYL-TRNA SYNTHETASE (PROLINE--TRNA LIGASE); Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). (501 aa) |
| | MYPU_2000 | GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A (GLU-ADT SUBUNIT A); Identified by sequence similarity; possible; similar to SWISS-PROT:P47345 (GATA_MYCGE) Blastp2 P=4e-68 C=54%. (448 aa) |
| | MYPU_2010 | Conserved hypothetical protein; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity); Belongs to the GatB/GatE family. GatB subfamily. (494 aa) |
| | MYPU_2030 | N-UTILIZATION SUBSTANCE PROTEIN A; Identified by sequence similarity; putative; similar to SWISS-PROT:P75591 (NUSA_MYCPN) Blastp2 P=2e-27 C=32%. (550 aa) |
| | MYPU_2040 | Conserved hypothetical protein; Identified by sequence similarity; putative; similar to SWISS-PROT:P32728 (YLXR_BACSU) Blastp2 P=5e-06 C=43%. (103 aa) |
| | MYPU_2050 | TRANSLATION INITIATION FACTOR IF-2; One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (603 aa) |
| | MYPU_2070 | LIPOPROTEIN; No similarity found Pfscan: pos. 6-16 PS00013 | PROKAR_LIPOPROTEIN Prokaryotic membrane lipoprotein lipid attachment site. (567 aa) |
| | MYPU_2090 | TRIGGER FACTOR (PROLYL ISOMERASE); Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase; Belongs to the FKBP-type PPIase family. Tig subfamily. (459 aa) |
| | MYPU_2190 | RIBOSOME RECYCLING FACTOR (RIBOSOME RELEASING FACTOR); Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another; Belongs to the RRF family. (187 aa) |
| | MYPU_2200 | URIDYLATE KINASE (UK) (URIDINE MONOPHOSPHATE KINASE) (UMP KINASE); Catalyzes the reversible phosphorylation of UMP to UDP. (243 aa) |
| | MYPU_2230 | HEAT SHOCK PROTEIN DNAK; Acts as a chaperone; Belongs to the heat shock protein 70 family. (599 aa) |
| | MYPU_2350 | ATP SYNTHASE ALPHA CHAIN; Identified by sequence similarity; possible; similar to SWISS-PROT:P33252 (ATPA_MYCGA) Blastp2 P=2e-89 C=50%. (529 aa) |
| | MYPU_2360 | ATP SYNTHASE BETA CHAIN; Identified by sequence similarity; possible; similar to SWISS-PROT:O50292 (ATPB_AQUPY) Blastp2 P=4e-91 C=37%. (698 aa) |
| | MYPU_2390 | GTP-BINDING PROTEIN; Necessary for normal cell division and for the maintenance of normal septation; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family. (186 aa) |
| | MYPU_2430 | ARGINYL-TRNA SYNTHETASE (ARGININE--TRNA LIGASE) (ARGRS); Identified by sequence similarity; possible; similar to SWISS-PROT:P46906 (SYR_BACSU) Blastp2 P=3e-97 C=52%; Belongs to the class-I aminoacyl-tRNA synthetase family. (534 aa) |
| | MYPU_2470 | GTP-BINDING PROTEIN LEPA; Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner. (611 aa) |
| | MYPU_2570 | VALYL-TRNA SYNTHETASE (VALINE--TRNA LIGASE) (VALRS); Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (827 aa) |
| | MYPU_2590 | TRANSLATION INITIATION FACTOR IF-3; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (236 aa) |
| | MYPU_2600 | 50S RIBOSOMAL PROTEIN L35; Identified by sequence similarity; probable; similar to SWISS-PROT:Q05428 (RL35_MYCFE) Blastp2 P=9e-14 C=63%; Belongs to the bacterial ribosomal protein bL35 family. (64 aa) |
| | MYPU_2610 | 50S RIBOSOMAL PROTEIN L20; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit. (116 aa) |
| | MYPU_2650 | ATP SYNTHASE EPSILON CHAIN; Produces ATP from ADP in the presence of a proton gradient across the membrane. (141 aa) |
| | MYPU_2660 | ATP SYNTHASE BETA CHAIN; Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits; Belongs to the ATPase alpha/beta chains family. (475 aa) |
| | MYPU_2670 | ATP SYNTHASE GAMMA CHAIN; Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. (279 aa) |
| | MYPU_2680 | ATP SYNTHASE ALPHA CHAIN; Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit. Belongs to the ATPase alpha/beta chains family. (528 aa) |
| | MYPU_2690 | ATP SYNTHASE DELTA CHAIN; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation; Belongs to the ATPase delta chain family. (179 aa) |
| | MYPU_2700 | ATP SYNTHASE B CHAIN; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. (180 aa) |
| | MYPU_2710 | ATP SYNTHASE C CHAIN; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. (92 aa) |
| | MYPU_2720 | ATP SYNTHASE A CHAIN; Identified by sequence similarity; putative; similar to SWISS-PROT:P33251 (ATP6_MYCGA) Blastp2 P=3e-18 C=36%. (270 aa) |
| | MYPU_2890 | 50S RIBOSOMAL PROTEIN L7/L12; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family. (122 aa) |
| | MYPU_2900 | 50S RIBOSOMAL PROTEIN L10; Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. Belongs to the universal ribosomal protein uL10 family. (186 aa) |
| | MYPU_2980 | RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE D (PSEUDOURIDYLATE SYNTHASE) (URACIL HYDROLYASE); Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (296 aa) |
| | MYPU_3040 | TRNA/RRNA METHYLTRANSFERASE; Identified by sequence similarity; possible; similar to SWISS-PROT:P74261 (YG73_SYNY3) Blastp2 P=2e-14 C=46%. (250 aa) |
| | MYPU_3060 | GTP-BINDING PROTEIN; Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity; Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily. (291 aa) |
| | MYPU_3240 | 50S RIBOSOMAL PROTEIN L32; Identified by sequence similarity; putative; similar to TREMBL:O84816 (O84816) Blastp2 P=6e-06 C=37%; Belongs to the bacterial ribosomal protein bL32 family. (61 aa) |
| | MYPU_3280 | 30S RIBOSOMAL PROTEIN S15; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA. (88 aa) |
| | MYPU_3290 | 50S RIBOSOMAL PROTEIN L27; Identified by sequence similarity; probable; similar to SWISS-PROT:P05657 (RL27_BACSU) Blastp2 P=1e-24 C=73%; Belongs to the bacterial ribosomal protein bL27 family. (85 aa) |
| | MYPU_3300 | 50S RIBOSOMAL PROTEIN L21; This protein binds to 23S rRNA in the presence of protein L20; Belongs to the bacterial ribosomal protein bL21 family. (131 aa) |
| | MYPU_3320 | ASPARTYL-TRNA SYNTHETASE (ASPARTATE--TRNA LIGASE) (ASPRS); Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (578 aa) |
| | MYPU_3330 | HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)h); Identified by sequence similarity; possible; similar to SWISS-PROT:P51348 (SYH_PORPU) Blastp2 P=1e-54 C=38%. (420 aa) |
| | MYPU_3420 | SIGNAL RECOGNITION PARTICLE PROTEIN (FIFTY-FOUR HOMOLOG); Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Belongs to the GTP-binding SRP family. SRP54 subfamily. (449 aa) |
| | MYPU_3470 | PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN (PHENYLALANINE--TRNA LIGASE BETA CHAIN) (PHERS); Identified by sequence similarity; possible; similar to SWISS-PROT:P75563 (SYFB_MYCPN) Blastp2 P=3e-43 C=46%; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (718 aa) |
| | MYPU_3480 | Phenylalanine--tRNA ligase alpha subunit; Identified by sequence similarity; possible; similar to SWISS-PROT:P17921 (SYFA_BACSU) Blastp2 P=9e-58 C=57%; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (314 aa) |
| | MYPU_3490 | Unknown; No similarity found. (237 aa) |
| | MYPU_3500 | Unknown; No similarity found. (90 aa) |
| | MYPU_3510 | VACB-LIKE (Shigella flexneri) RIBONUCLEASE II; 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs. (725 aa) |
| | MYPU_3520 | SMALL PROTEIN B; Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to tran [...] (145 aa) |
| | MYPU_3530 | GTP-BINDING PROTEIN; An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control. Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. (418 aa) |
| | MYPU_3540 | Unknown; No similarity found. (41 aa) |
| | MYPU_3580 | ACYL CARRIER PROTEIN (ACP); Carrier of the growing fatty acid chain in fatty acid biosynthesis. (73 aa) |
| | MYPU_3890 | DNA LIGASE (POLYDEOXYRIBONUCLEOTIDE SYNTHASE [NAD+]); DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. (679 aa) |
| | MYPU_3900 | LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS); Identified by sequence similarity; probable; similar to SWISS-PROT:Q49158 (SYK_MYCFE) Blastp2 P=0 C=70%; Belongs to the class-II aminoacyl-tRNA synthetase family. (510 aa) |
| | MYPU_4010 | DNA LIGASE (POLYDEOXYRIBONUCLEOTIDE SYNTHASE [NAD+]) (FRAGMENT); Identified by sequence similarity; possible; similar to SWISS-PROT:P78021 (DNLJ_MYCPN) Blastp2 P=2e-48 C=52%. (367 aa) |
| | MYPU_4020 | LYSYL-TRNA SYNTHETASE (LYSINE--TRNA LIGASE) (LYSRS); Identified by sequence similarity; probable; similar to SWISS-PROT:Q49158 (SYK_MYCFE) Blastp2 P=0 C=73%; Belongs to the class-II aminoacyl-tRNA synthetase family. (491 aa) |
| | MYPU_4050 | ELONGATION FACTOR TU (EF-TU); This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa) |
| | MYPU_4280 | ELONGATION FACTOR G (EF-G); Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity); Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. [...] (695 aa) |
| | MYPU_4290 | 30S RIBOSOMAL PROTEIN S7; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | MYPU_4300 | 30S RIBOSOMAL PROTEIN S12; With S4 and S5 plays an important role in translational accuracy. (137 aa) |
| | MYPU_4490 | ATP SYNTHASE ALPHA CHAIN; Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit. Belongs to the ATPase alpha/beta chains family. (513 aa) |
| | MYPU_4500 | ATP SYNTHASE BETA CHAIN; Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits; Belongs to the ATPase alpha/beta chains family. (468 aa) |
| | MYPU_4670 | 50S RIBOSOMAL PROTEIN L19; This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. (117 aa) |
| | MYPU_4680 | TRNA (GUANINE-N1)-METHYLTRANSFERASE (M1G-METHYLTRANSFERASE) (TRNA [GM37] METHYLTRANSFERASE); Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (225 aa) |
| | MYPU_4690 | 30S RIBOSOMAL PROTEIN S16; Identified by sequence similarity; probable; similar to SWISS-PROT:P21474 (RS16_BACSU) Blastp2 P=3e-20 C=68%; Belongs to the bacterial ribosomal protein bS16 family. (88 aa) |
| | MYPU_4700 | INORGANIC PYROPHOSPHATASE (PYROPHOSPHATE PHOSPHO-HYDROLASE) (PPASE); Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions. (183 aa) |
| | MYPU_4710 | DNA TOPOISOMERASE I (OMEGA-PROTEIN) (RELAXING ENZYME) (UNTWISTING ENZYME) (SWIVELASE); Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then un [...] (625 aa) |
| | MYPU_4850 | TYROSYL-TRNA SYNTHETASE 1 (TYROSINE--TRNA LIGASE) (TYRRS 1); Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (414 aa) |
| | MYPU_4860 | PHENYLALANYL-TRNA SYNTHETASE, BETA CHAIN (PHENYLALANINE--TRNA LIGASE BETA CHAIN) (PHERS); Identified by sequence similarity; putative; similar to TREMBL:O34943 (O34943) Blastp2 P=1e-06 C=41%. (196 aa) |
| | MYPU_4890 | 50S RIBOSOMAL PROTEIN L33; Identified by sequence similarity; probable; similar to SWISS-PROT:O67756 (RL33_AQUAE) Blastp2 P=4e-09 C=60%; Belongs to the bacterial ribosomal protein bL33 family. (50 aa) |
| | MYPU_4900 | METHIONYL-TRNA SYNTHETASE (METHIONINE--TRNA LIGASE) (METRS); Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation; Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. (509 aa) |
| | MYPU_4950 | 50S RIBOSOMAL PROTEIN L13; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (144 aa) |
| | MYPU_4960 | 30S RIBOSOMAL PROTEIN S9; Identified by sequence similarity; probable; similar to SWISS-PROT:P21470 (RS9_BACSU) Blastp2 P=4e-34 C=66%; Belongs to the universal ribosomal protein uS9 family. (136 aa) |
| | MYPU_5020 | Unknown; No similarity found. (326 aa) |
| | MYPU_5060 | PEPTIDE METHIONINE SULFOXIDE REDUCTASE (PEPTIDE MET(O) REDUCTASE); Identified by sequence similarity; probable; similar to SWISS-PROT:O25011 (PMSR_HELPY) Blastp2 P=1e-44 C=67%. (145 aa) |
| | MYPU_5130 | ELONGATION FACTOR P (EF-P); Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. (187 aa) |
| | MYPU_5160 | 50S RIBOSOMAL PROTEIN L9; Binds to the 23S rRNA. (156 aa) |
| | MYPU_5190 | HEAT SHOCK ATP-DEPENDENT PROTEASE; Identified by sequence similarity; possible; similar to SWISS-PROT:P36772 (LON_BACBR) Blastp2 P=0 C=56%. (842 aa) |
| | MYPU_5320 | ELONGATION FACTOR TS (EF-TS); Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity); Belongs to the EF-Ts family. (294 aa) |
| | MYPU_5330 | 30S RIBOSOMAL PROTEIN S2; Identified by sequence similarity; possible; similar to SWISS-PROT:P21464 (RS2_BACSU) Blastp2 P=1e-63 C=50%; Belongs to the universal ribosomal protein uS2 family. (330 aa) |
| | MYPU_5450 | DNA-DIRECTED RNA POLYMERASE BETA' CHAIN (TRANSCRIPTASE BETA' CHAIN) (RNA POLYMERASE BETA' SUBUNIT); DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1426 aa) |
| | MYPU_5460 | DNA-DIRECTED RNA POLYMERASE BETA CHAIN (TRANSCRIPTASE BETA CHAIN) (RNA POLYMERASE BETA SUBUNIT); DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1216 aa) |
| | MYPU_5500 | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (282 aa) |
| | MYPU_5520 | DEOXYGUANOSINE KINASE (DGUO KINASE) (DGK) (DEOXYNUCLEOSIDE KINASE COMPLEX I F-COMPONENT); Identified by sequence similarity; possible; similar to SWISS-PROT:P75396 (Y268_MYCPN) Blastp2 P=9e-21 C=50% Pfscan: pos. 7-14 PS00017 | ATP_GTP_A ATP/GTP-binding site motif A (P-loop). (216 aa) |
| | MYPU_5530 | TRANSCRIPTION ELONGATION FACTOR GREA (TRANSCRIPT CLEAVAGE FACTOR GREA); Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (164 aa) |
| | MYPU_5540 | Unknown; No similarity found. (226 aa) |
| | MYPU_5550 | Unknown; No similarity found Pfscan: pos. 29-84 GC0265 | CARBOXYLESTERASE_B Carboxylesterases type-B active site. (112 aa) |
| | MYPU_5560 | Conserved hypothetical protein; Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA; Belongs to the YqgF nuclease family. (146 aa) |
| | MYPU_5570 | ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE) (ALARS); Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain. (873 aa) |
| | MYPU_5580 | TRNA (5-METHYLAMINOMETHYL-2-THIOURIDYLATE)-METHYLTRANSFERASE; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. (371 aa) |
| | MYPU_5590 | Unknown; No similarity found. (1228 aa) |
| | MYPU_5600 | 50S RIBOSOMAL PROTEIN L17; Identified by sequence similarity; probable; similar to SWISS-PROT:P07843 (RL17_BACST) Blastp2 P=2e-23 C=62%. (120 aa) |
| | MYPU_5610 | DNA-directed RNA polymerase subunit alpha; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (333 aa) |
| | MYPU_5620 | 30S RIBOSOMAL PROTEIN S11; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (136 aa) |
| | MYPU_5630 | 30S RIBOSOMAL PROTEIN S13; Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. Belongs to the universal ribosomal protein uS13 family. (132 aa) |
| | MYPU_5640 | RIBOSOMAL PROTEIN L36; Identified by sequence similarity; similar to SWISSPROT:P46361 (RL36_HAEIN) Blast2p P=3e-4 score=41; Belongs to the bacterial ribosomal protein bL36 family. (37 aa) |
| | MYPU_5650 | TRANSLATION INITIATION FACTOR IF-1; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (72 aa) |
| | MYPU_5660 | METHIONINE AMINOPEPTIDASE (MAP) (PEPTIDASE M); Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed; Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. (249 aa) |
| | MYPU_5670 | ADENYLATE KINASE (ATP-AMP TRANSPHOSPHORYLASE); Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism; Belongs to the adenylate kinase family. (214 aa) |
| | MYPU_5680 | PREPROTEIN TRANSLOCASE SECY SUBUNIT; The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. (474 aa) |
| | MYPU_5690 | 50S RIBOSOMAL PROTEIN L15; Binds to the 23S rRNA; Belongs to the universal ribosomal protein uL15 family. (145 aa) |
| | MYPU_5700 | 30S RIBOSOMAL PROTEIN S5; With S4 and S12 plays an important role in translational accuracy; Belongs to the universal ribosomal protein uS5 family. (229 aa) |
| | MYPU_5710 | 50S RIBOSOMAL PROTEIN L18; This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. (118 aa) |
| | MYPU_5720 | 50S RIBOSOMAL PROTEIN L6; This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center; Belongs to the universal ribosomal protein uL6 family. (179 aa) |
| | MYPU_5730 | 30S RIBOSOMAL PROTEIN S8; One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; Belongs to the universal ribosomal protein uS8 family. (132 aa) |
| | MYPU_5740 | RIBOSOMAL PROTEIN S14; Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. (61 aa) |
| | MYPU_5750 | 50S RIBOSOMAL PROTEIN L5; This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. (181 aa) |
| | MYPU_5760 | 50S RIBOSOMAL PROTEIN L24; One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (108 aa) |
| | MYPU_5770 | 50S RIBOSOMAL PROTEIN L14; Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome; Belongs to the universal ribosomal protein uL14 family. (131 aa) |
| | MYPU_5780 | 30S RIBOSOMAL PROTEIN S17; One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. (88 aa) |
| | MYPU_5790 | 50S RIBOSOMAL PROTEIN L29; Identified by sequence similarity; probable; similar to SWISS-PROT:P04457 (RL29_BACST) Blastp2 P=3e-10 C=64%; Belongs to the universal ribosomal protein uL29 family. (65 aa) |
| | MYPU_5800 | 50S RIBOSOMAL PROTEIN L16; Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs; Belongs to the universal ribosomal protein uL16 family. (142 aa) |
| | MYPU_5810 | 30S RIBOSOMAL PROTEIN S3; Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation; Belongs to the universal ribosomal protein uS3 family. (217 aa) |
| | MYPU_5830 | 50S RIBOSOMAL PROTEIN L22; This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (110 aa) |
| | MYPU_5840 | 30S RIBOSOMAL PROTEIN S19; Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (91 aa) |
| | MYPU_5850 | 50S RIBOSOMAL PROTEIN L2; One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Belongs to the universal ribosomal protein uL2 family. (281 aa) |
| | MYPU_5860 | 50S RIBOSOMAL PROTEIN L23; One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome; Belongs to the universal ribosomal protein uL23 family. (154 aa) |
| | MYPU_5870 | 50S RIBOSOMAL PROTEIN L4; One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. (292 aa) |
| | MYPU_5880 | Unknown; No similarity found. (316 aa) |
| | MYPU_5890 | 50S RIBOSOMAL PROTEIN L3; One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit; Belongs to the universal ribosomal protein uL3 family. (266 aa) |
| | MYPU_5900 | 30S RIBOSOMAL PROTEIN S10; Involved in the binding of tRNA to the ribosomes. Belongs to the universal ribosomal protein uS10 family. (103 aa) |
| | MYPU_6090 | 30S RIBOSOMAL PROTEIN S18; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (105 aa) |
| | MYPU_6110 | 30S RIBOSOMAL PROTEIN S6; Binds together with S18 to 16S ribosomal RNA. (150 aa) |
| | MYPU_6560 | 30S RIBOSOMAL PROTEIN S20; Binds directly to 16S ribosomal RNA. (110 aa) |
| | MYPU_6590 | PROTEIN-EXPORT MEMBRANE PROTEIN SECD; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. (873 aa) |
| | MYPU_6670 | ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS); Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (888 aa) |
| | MYPU_6720 | RIBOSOME-BINDING FACTOR A; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (112 aa) |
| | MYPU_6740 | CELL DIVISION PROTEIN FTSY; Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). (373 aa) |
| | MYPU_6820 | GLUTAMYL-TRNA SYNTHETASE (GLUTAMATE--TRNA LIGASE) (GLURS); Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu); Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. (468 aa) |
| | MYPU_6890 | POLYPEPTIDE DEFORMYLASE 2 (PDF 2) (FORMYLMETHIONINE DEFORMYLASE 2); Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. (198 aa) |
| | MYPU_6900 | ABC TRANSPORTER ATP-BINDING PROTEIN; Identified by sequence similarity; possible; similar to SWISS-PROT:P75790 (YBIT_ECOLI) Blastp2 P=0 C=63% Pfscan: pos. 29-54 PS50101 | ATP_GTP_A2 P-loop nucleotide binding motif (does not find all). pos. 156-224 PS50100 | DA_BOX 2nd half motif for nucleotide binding, associated with P-loop. pos. 447-515 PS50100 | DA_BOX 2nd half motif for nucleotide binding, associated with P-loop. pos. 27-228 PF00005 | ABC_TRAN ABC transporter (PDOC00185) pos. 346-519 PF00005 | ABC_TRAN ABC transporter (PDOC00185) pos. 425-478 GC0425 | ATP_BIND_TRANSPORT ATP-binding [...] (537 aa) |
| | MYPU_6950 | ESTERASE/LIPASE; Identified by sequence similarity; similar to TREMBL:P75268 Blas2p P=0.11 score=38 Pfscan: pos. 24-112 PS50187 | ESTERASE Esterase/lipase/thioesterase active site serine. (272 aa) |
| | MYPU_6960 | 50S RIBOSOMAL PROTEIN L28; Identified by sequence similarity; probable; similar to SWISS-PROT:P47665 (RL28_MYCGE) Blastp2 P=2e-11 C=70%; Belongs to the bacterial ribosomal protein bL28 family. (63 aa) |
| | MYPU_6990 | ATP SYNTHASE BETA CHAIN; Identified by sequence similarity; probable; similar to SWISS-PROT:P43451 (ATPB_ENTHR) Blastp2 P=1e-99 C=59%. (468 aa) |
| | MYPU_7000 | ATP SYNTHASE ALPHA CHAIN; Identified by sequence similarity; possible; similar to SWISS-PROT:P29706 (ATPA_PROMO) Blastp2 P=2e-62 C=45%. (509 aa) |
| | MYPU_7160 | 30S RIBOSOMAL PROTEIN S4; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. (199 aa) |
| | MYPU_7330 | HEAT SHOCK PROTEIN DNAJ (activation of DNAK); Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent intera [...] (383 aa) |
| | MYPU_7460 | THREONYL-TRNA SYNTHETASE 1 (THREONINE--TRNA LIGASE) (THRRS); Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (589 aa) |
| | MYPU_7710 | ASPARAGINYL-TRNA SYNTHETASE (ASPARAGINE--TRNA LIGASE) (ASNRS); Identified by sequence similarity; probable; similar to SWISS-PROT:O51128 (SYN_BORBU) Blastp2 P=0 C=70%. (450 aa) |
| | MYPU_7780 | PEPTIDE METHIONINE SULFOXIDE REDUCTASE (PEPTIDE MET(O) REDUCTASE); Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. (177 aa) |
| | MYPU_7790 | CELL DIVISION PROTEIN FTSH; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins; In the central section; belongs to the AAA ATPase family. (725 aa) |
