Your Input: | |
| | SerS | Seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (425 aa) |
| | MetG | Methionyl-tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (638 aa) |
| | Rho | Transcription termination factor; Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. (488 aa) |
| | RpmE | Ribosomal protein L31; Binds the 23S rRNA. (69 aa) |
| | LeuS | Leucyl-tRNA synthetase; Best Blastp hit = gi|6919980|sp|Q9WY15|SYL_THEMA LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS) gi|7437543|pir||F72408 leucine--tRNA ligase (EC 6.1.1.4) - Thermotoga maritima (strain MSB8) gi|4980663|gb|AAD35261.1|AE001702_1 (AE001702) leucyl-tRNA synthetase [Thermotoga maritima], score 840, E-value 0; Belongs to the class-I aminoacyl-tRNA synthetase family. (819 aa) |
| | hpf | Ribosome-associated protein Y (PSrp-1); Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth. (184 aa) |
| | SecA | Preprotein translocase subunit SecA (ATPase, RNA helicase); Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane; Belongs to the SecA family. (901 aa) |
| | PrfB | Protein chain release factor B; Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (347 aa) |
| | Uup | ATPase components of ABC transporters with duplicated ATPase domains; Best Blastp hit = gi|3025120|sp|O05519|YDIF_BACSU HYPOTHETICAL ABC TRANSPORTER ATP-BINDING PROTEIN YDIF gi|7445765|pir||G69786 ABC transporter (ATP-binding protein) homolog ydiF - Bacillus subtilis gi|1945111|dbj|BAA19719.1| (D88802) H. influenzae hypothetical ABC transporter; P44808 (974) [Bacillus subtilis] gi|2632908|emb|CAB12414.1| (Z99107) similar to ABC transporter (ATP-binding protein) [Bacillus subtilis], score 467, E-value 1.00E-130. (630 aa) |
| | AtpB | F0F1-type ATP synthase a subunit; Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane. (217 aa) |
| | AtpE | F0F1-type ATP synthase c subunit/Archaeal/vacuolar-type H+-ATPase subunit K; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. (73 aa) |
| | AtpF | F0F1-type ATP synthase b subunit; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. (168 aa) |
| | AtpH | F0F1-type ATP synthase delta subunit; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation; Belongs to the ATPase delta chain family. (177 aa) |
| | AtpA | F0F1-type ATP synthase alpha subunit; Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit. Belongs to the ATPase alpha/beta chains family. (506 aa) |
| | AtpG | F0F1-type ATP synthase gamma subunit; Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. (295 aa) |
| | AtpD | F0F1-type ATP synthase beta subunit; Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits; Belongs to the ATPase alpha/beta chains family. (461 aa) |
| | AtpC | F0F1-type ATP synthase epsilon subunit; Produces ATP from ADP in the presence of a proton gradient across the membrane. (138 aa) |
| | NusG | Transcription antiterminator; Participates in transcription elongation, termination and antitermination. (171 aa) |
| | Ndk | Nucleoside diphosphate kinase; Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate; Belongs to the NDK family. (139 aa) |
| | ValS | Valyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (879 aa) |
| | TypA | Predicted membrane GTPase involved in stress response; Best Blastp hit = gi|10175253|dbj|BAB06351.1| (AP001516) GTP-binding protein TypA/BipA (tyrosine phosphorylated protein A) [Bacillus halodurans], score 721, E-value 0. (606 aa) |
| | RplU | Ribosomal protein L21; This protein binds to 23S rRNA in the presence of protein L20; Belongs to the bacterial ribosomal protein bL21 family. (104 aa) |
| | RpmA | Ribosomal protein L27; Best Blastp hit = gi|13701443|dbj|BAB42737.1| (AP003134) 50S ribosomal protei L27 (BL30) [Staphylococcus aureus], score 134, E-value 4.00E-31; Belongs to the bacterial ribosomal protein bL27 family. (95 aa) |
| | GlnS | Glutamyl- and glutaminyl-tRNA synthetases; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu); Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. (481 aa) |
| | RpsT | Ribosomal protein S20; Binds directly to 16S ribosomal RNA. (105 aa) |
| | RpsU | Ribosomal protein S21; Best Blastp hit = gi|13959513|sp|Q9CIW6|RS21_LACLA 30S RIBOSOMAL PROTEIN S21 gi|12723098|gb|AAK04338.1|AE006261_9 (AE006261) 30S ribosomal protein S21 [Lactococcus lactis subsp. lactis], score 53.9, E-value 3.00E-07; Belongs to the bacterial ribosomal protein bS21 family. (73 aa) |
| | SmpB | tmRNA-binding protein; Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches t [...] (158 aa) |
| | YajC | Preprotein translocase subunit YajC; Best Blastp hit = gi|13634024|sp|Q9WZW3|Y859_THEMA HYPOTHETICAL PROTEIN TM0859 gi|7462565|pir||D72325 hypothetical protein - Thermotoga maritima (strain MSB8) gi|4981393|gb|AAD35941.1|AE001752_8 (AE001752) hypothetical protein [Thermotoga maritima], score 53.5, E-value 6.00E-07. (101 aa) |
| | HisS | Histidyl-tRNA synthetase; Best Blastp hit = gi|7674351|sp|Q9X0H5|SYH_THEMA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS) gi|7437817|pir||A72298 histidine--tRNA ligase (EC 6.1.1.21) - Thermotoga maritima (strain MSB8) gi|4981634|gb|AAD36166.1|AE001768_15 (AE001768) histidyl-tRNA synthetase [Thermotoga maritima], score 400, E-value 1.00E-110. (417 aa) |
| | AspS | Aspartyl-tRNA synthetase; Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn); Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (592 aa) |
| | RpsA | Ribosomal protein S1; Best Blastp hit = gi|7473661|pir||B75330 probable ribosomal protein S1 - Deinococcus radiodurans (strain R1) gi|6459772|gb|AAF11535.1|AE002036_6 '(AE002036) ribosomal protein S1, putative [Deinococcus radiodurans]', score 185, E-value 9.00E-46. (367 aa) |
| | RpsO | Ribosomal protein S15P/S13E; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA. (88 aa) |
| | RibF | FAD synthase; Best Blastp hit = gi|7444639|pir||D70313 riboflavin kinase - Aquifex aeolicus gi|2982868|gb|AAC06488.1| (AE000675) riboflavin kinase [Aquifex aeolicus], score 193, E-value 3.00E-48; Belongs to the ribF family. (316 aa) |
| | TruB | Pseudouridine synthase; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (285 aa) |
| | TTE1391 | Exopolyphosphatase-related proteins; Best Blastp hit = gi|7445472|pir||F70440 conserved hypothetical protein aq_1630 - Aquifex aeolicus gi|2983973|gb|AAC07520.1| (AE000748) hypothetical protein [Aquifex aeolicus], score 186, E-value 3.00E-46. (317 aa) |
| | RbfA | Ribosome-binding factor A; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (122 aa) |
| | InfB | Translation initiation factor 2 (GTPase); One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (707 aa) |
| | RPL8A | Best Blastp hit = gi|418462|sp|P32729|YLXQ_BACSU PROBABLE RIBOSOMAL PROTEIN IN NUSA-INFB INTERGENIC REGION (ORF4) gi|541415|pir||E36905 ribosomal protein L7AE family homolog ylxQ - Bacillus subtilis gi|49318|emb|CAA79233.1| (Z18631) ORF4 [Bacillus subtilis] gi|2634034|emb|CAB13535.1| (Z99112) alternate gene name: ymxC; similar to ribosomal protein L7AE family [Bacillus subtilis], score 65.5, E-value 2.00E-10. (107 aa) |
| | TTE1395 | Predicted nucleic-acid-binding protein implicated in transcription termination; Best Blastp hit = gi|10175035|dbj|BAB06134.1| (AP001515) BH2415; unknown conserved protein [Bacillus halodurans], score 80.5, E-value 4.00E-15. (90 aa) |
| | NusA | Transcription terminator; Participates in both transcription termination and antitermination. (346 aa) |
| | rimP | Conserved hypothetical protein; Required for maturation of 30S ribosomal subunits. Belongs to the RimP family. (151 aa) |
| | TTE1401 | Predicted membrane-associated Zn-dependent protease 1; Best Blastp hit = gi|7474650|pir||C69881 conserved hypothetical protein yluC - Bacillus subtilis gi|2634028|emb|CAB13529.1| (Z99112) similar to hypothetical proteins [Bacillus subtilis], score 158, E-value 1.00E-37. (332 aa) |
| | CdsA | CDP-diglyceride synthetase; Best Blastp hit = gi|10175042|dbj|BAB06141.1| (AP001515) phosphatidate cytidylyltransferase [Bacillus halodurans], score 135, E-value 5.00E-31; Belongs to the CDS family. (261 aa) |
| | UppS | Undecaprenyl pyrophosphate synthase; Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids. (247 aa) |
| | TTE1405 | Hypothetical protein. (58 aa) |
| | Frr | Ribosome recycling factor; Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another; Belongs to the RRF family. (184 aa) |
| | PyrH | Uridylate kinase; Catalyzes the reversible phosphorylation of UMP to UDP. (237 aa) |
| | Tsf | Translation elongation factor Ts; Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Belongs to the EF-Ts family. (200 aa) |
| | RpsB | Ribosomal protein S2; Best Blastp hit = gi|10175047|dbj|BAB06146.1| (AP001515) 30S ribosomal protein S2 [Bacillus halodurans], score 367, E-value 1.00E-100; Belongs to the universal ribosomal protein uS2 family. (235 aa) |
| | TrpS | Tryptophanyl-tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (327 aa) |
| | TTE1454 | Conserved hypothetical protein; Best Blastp hit = gi|7450571|pir||A70102 conserved hypothetical integral membrane protein BB0017 - Lyme disease spirochete gi|2687904|gb|AAC66414.1| (AE001116) conserved hypothetical integral membrane protein [Borrelia burgdorferi], score 209, E-value 5.00E-53. (294 aa) |
| | TTE1456 | Predicted GTPases; Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity; Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily. (277 aa) |
| | RplS | Ribosomal protein L19; This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. (115 aa) |
| | TrmD | tRNA-(guanine-N1)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (241 aa) |
| | RimM | RimM protein, required for 16S rRNA processing; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (168 aa) |
| | TTE1460 | Predicted RNA-binding protein (KH domain); Best Blastp hit = gi|7388372|sp|O66524|Y124_AQUAE HYPOTHETICAL PROTEIN AQ_124A gi|7430171|pir||A70312 conserved hypothetical protein aq_124a - Aquifex aeolicus gi|2982883|gb|AAC06503.1| (AE000675) hypothetical protein [Aquifex aeolicus], score 81.6, E-value 1.00E-15; Belongs to the UPF0109 family. (75 aa) |
| | RpsP | Ribosomal protein S16; Best Blastp hit = gi|585937|sp|P21474|RS16_BACSU 30S RIBOSOMAL PROTEIN S16 (BS17) gi|477949|pir||C47154 ribosomal protein S16 (BS17) rpsP - Bacillus subtilis gi|2309081|dbj|BAA21692.1| (D14356) 30S ribosomal protein S16 [Bacillus subtilis] gi|2633971|emb|CAB13472.1| (Z99112) ribosomal protein S16 (BS17) [Bacillus subtilis], score 122, E-value 6.00E-28; Belongs to the bacterial ribosomal protein bS16 family. (82 aa) |
| | Ffh | Signal recognition particle GTPase; Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Belongs to the GTP-binding SRP family. SRP54 subfamily. (447 aa) |
| | TTE1463 | Conserved hypothetical protein; Might take part in the signal recognition particle (SRP) pathway. This is inferred from the conservation of its genetic proximity to ftsY/ffh. May be a regulatory protein (By similarity). (117 aa) |
| | FtsY | Signal recognition particle GTPase; Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). (295 aa) |
| | TTE1466 | Hypothetical protein. (77 aa) |
| | SpoVS3 | Conserved hypothetical protein; Best Blastp hit = gi|10174995|dbj|BAB06094.1| (AP001515) stage V sporulation protein S [Bacillus halodurans], score 83.2, E-value 5.00E-16. (86 aa) |
| | RpmB | Ribosomal protein L28; Best Blastp hit = gi|8928328|sp|O50656|RL28_SELRU 50S RIBOSOMAL PROTEIN L28 gi|2897823|dbj|BAA24922.1| (AB011029) similar to ribosomal protein L28 [Selenomonas ruminantium], score 77.4, E-value 2.00E-14; Belongs to the bacterial ribosomal protein bL28 family. (62 aa) |
| | RpoZ | DNA-directed RNA polymerase subunit K/omega; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (70 aa) |
| | IleS | Isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (932 aa) |
| | ychF | Predicted GTPase; ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner. (363 aa) |
| | PheT | Best Blastp hit = gi|3123292|sp|P17922|SYFB_BACSU PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN (PHENYLALANINE--TRNA LIGASE BETA CHAIN) (PHERS) gi|7428487|pir||YFBSB phenylalanine--tRNA ligase (EC 6.1.1.20) beta chain - Bacillus subtilis gi|1770031|emb|CAA99564.1| (Z75208) phenylalanyl-tRNA synthetase beta subunit [Bacillus subtilis] gi|2635328|emb|CAB14823.1| (Z99118) phenylalanyl-tRNA synthetase (beta subunit) [Bacillus subtilis], score 591, E-value 1.00E-167. (794 aa) |
| | PheS | Best Blastp hit = gi|2507429|sp|P17921|SYFA_BACSU PHENYLALANYL-TRNA SYNTHETASE ALPHA CHAIN (PHENYLALANINE--TRNA LIGASE ALPHA CHAIN) (PHERS) gi|7428485|pir||YFBSA phenylalanine--tRNA ligase (EC 6.1.1.20) alpha chain - Bacillus subtilis gi|1770030|emb|CAA99603.1| (Z75208) phenylalany-tRNA synthetase beta subunit [Bacillus subtilis] gi|2635329|emb|CAB14824.1| (Z99118) phenylalanyl-tRNA synthetase (alpha subunit) [Bacillus subtilis], score 421, E-value 1.00E-117; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (339 aa) |
| | RplT | Ribosomal protein L20; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit. (119 aa) |
| | RpmI | Ribosomal protein L35; Best Blastp hit = gi|6226000|sp|O66982|RL35_AQUAE 50S RIBOSOMAL PROTEIN L35 gi|7441200|pir||C70369 ribosomal protein L35 - Aquifex aeolicus gi|2983363|gb|AAC06950.1| (AE000708) ribosomal protein L35 [Aquifex aeolicus], score 52, E-value 1.00E-06; Belongs to the bacterial ribosomal protein bL35 family. (67 aa) |
| | InfC | Translation initiation factor IF3; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (180 aa) |
| | ThrS | Threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (644 aa) |
| | TTE1758 | Hypothetical protein; Involved in protein export. Participates in an early event of protein translocation; Belongs to the SecG family. (79 aa) |
| | SelB | Best Blastp hit = gi|5763687|emb|CAB53235.1| (AJ245960) elongation factor SelB [Eubacterium acidaminophilum], score 573, E-value 1.00E-162. (626 aa) |
| | RplY | Ribosomal protein L25 (general stress protein Ctc); This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance. Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily. (199 aa) |
| | HflB2 | ATP-dependent Zn proteases; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins; Belongs to the AAA ATPase family. In the central section; belongs to the AAA ATPase family. (510 aa) |
| | RpsA2 | Ribosomal protein S1; Best Blastp hit = gi|12723763|gb|AAK04936.1|AE006317_5 (AE006317) 30S ribosomal protein S1 [Lactococcus lactis subsp. lactis], score 85.1, E-value 9.00E-16. (257 aa) |
| | Gph | Predicted phosphatase; Best Blastp hit = gi|10176211|dbj|BAB07306.1| (AP001519) phosphoglycolate phosphatase [Bacillus halodurans], score 180, E-value 1.00E-44; Belongs to the HAD-like hydrolase superfamily. PpaX family. (245 aa) |
| | RpsI | Ribosomal protein S9; Best Blastp hit = gi|10172781|dbj|BAB03888.1| (AP001507) 30S ribosomal protein S9 [Bacillus halodurans], score 189, E-value 2.00E-47; Belongs to the universal ribosomal protein uS9 family. (130 aa) |
| | RplM | Ribosomal protein L13; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (143 aa) |
| | RplQ | Ribosomal protein L17; Best Blastp hit = gi|132718|sp|P20277|RL17_BACSU 50S RIBOSOMAL PROTEIN L17 (BL15) (BL21) gi|80370|pir||F32307 ribosomal protein L17 - Bacillus subtilis gi|142464|gb|AAA22218.1| (M26414) ribosomal protein L17 [Bacillus subtilis] gi|1044992|gb|AAB06827.1| (L47971) ribosomal protein L17 [Bacillus subtilis] gi|2632411|emb|CAB11920.1| (Z99104) ribosomal protein L17 (BL15) [Bacillus subtilis], score 160, E-value 6.00E-39. (112 aa) |
| | RpoA | DNA-directed RNA polymerase alpha subunit/40 kD subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (315 aa) |
| | RpsD | Ribosomal protein S4 and related proteins; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. (206 aa) |
| | RpsK | Ribosomal protein S11; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (130 aa) |
| | RpsM | Ribosomal protein S13; Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. Belongs to the universal ribosomal protein uS13 family. (122 aa) |
| | RpmJ | Ribosomal protein L36; Best Blastp hit = gi|132921|sp|P20278|RL36_BACSU 50S RIBOSOMAL PROTEIN L36 (RIBOSOMAL PROTEIN II) (RIBOSOMAL PROTEIN B) (BL38) gi|80372|pir||B32307 ribosomal protein L36 - Bacillus subtilis gi|142460|gb|AAA22214.1| (M26414) ribosomal protein B [Bacillus subtilis] gi|1044988|gb|AAB06823.1| (L47971) ribosomal protein B [Bacillus subtilis] gi|2632407|emb|CAB11916.1| (Z99104) ribosomal protein L36 (ribosomal protein B) [Bacillus subtilis], score 74.7, E-value 2.00E-13; Belongs to the bacterial ribosomal protein bL36 family. (37 aa) |
| | InfA | Translation initiation factor IF-1; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (72 aa) |
| | TTE2269 | Conserved hypothetical protein; Best Blastp hit = gi|2760180|dbj|BAA24189.1| (AB010082) unnamed protein product [Bacillus halodurans], score 50.1, E-value 5.00E-06. (88 aa) |
| | Map | Methionine aminopeptidase; Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed; Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. (248 aa) |
| | Adk | Adenylate kinase and related kinases; Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism; Belongs to the adenylate kinase family. (221 aa) |
| | SecY | Preprotein translocase subunit SecY; The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. (418 aa) |
| | RplO | Ribosomal protein L15; Binds to the 23S rRNA; Belongs to the universal ribosomal protein uL15 family. (147 aa) |
| | RpsE | Ribosomal protein S5; With S4 and S12 plays an important role in translational accuracy; Belongs to the universal ribosomal protein uS5 family. (167 aa) |
| | RplR | Ribosomal protein L18; This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. (121 aa) |
| | RplF | Ribosomal protein L6; This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center; Belongs to the universal ribosomal protein uL6 family. (180 aa) |
| | RpsH | Ribosomal protein S8; One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; Belongs to the universal ribosomal protein uS8 family. (132 aa) |
| | RpsN | Ribosomal protein S14; Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. (61 aa) |
| | RplE | Ribosomal protein L5; This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. (179 aa) |
| | RplX | Ribosomal protein L24; One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (107 aa) |
| | RplN | Ribosomal protein L14; Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome; Belongs to the universal ribosomal protein uL14 family. (122 aa) |
| | RpsQ | Ribosomal protein S17; One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. (84 aa) |
| | RpmC | Ribosomal protein L29; Best Blastp hit = gi|12643297|sp|Q9Z9K6|RL29_BACHD 50S RIBOSOMAL PROTEIN L29 gi|10172754|dbj|BAB03861.1| (AP001507) 50S ribosomal protein L29 [Bacillus halodurans], score 94, E-value 2.00E-19; Belongs to the universal ribosomal protein uL29 family. (66 aa) |
| | RplP | Ribosomal protein L16/L10E; Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs; Belongs to the universal ribosomal protein uL16 family. (144 aa) |
| | RpsC | Ribosomal protein S3; Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation; Belongs to the universal ribosomal protein uS3 family. (221 aa) |
| | RplV | Ribosomal protein L22; This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (112 aa) |
| | RpsS | Ribosomal protein S19; Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (93 aa) |
| | RplB | Ribosomal protein L2; One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Belongs to the universal ribosomal protein uL2 family. (275 aa) |
| | RplW | Ribosomal protein L23; One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome; Belongs to the universal ribosomal protein uL23 family. (96 aa) |
| | RplD | Ribosomal protein L4; One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. (206 aa) |
| | RplC | Ribosomal protein L3; One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit; Belongs to the universal ribosomal protein uL3 family. (210 aa) |
| | RpsJ | Ribosomal protein S10; Involved in the binding of tRNA to the ribosomes. Belongs to the universal ribosomal protein uS10 family. (102 aa) |
| | TufB | GTPases - translation elongation factors; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (400 aa) |
| | FusA | Translation elongation and release factors (GTPases); Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPas [...] (690 aa) |
| | RpsG | Ribosomal protein S7; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | RpsL | Ribosomal protein S12; With S4 and S5 plays an important role in translational accuracy. (124 aa) |
| | RPL8A.2 | Ribosomal protein HS6-type (S12/L30/L7a); Best Blastp hit = gi|11276720|pir||T44377 hypothetical protein ybxF [imported] - Bacillus halodurans gi|4512398|dbj|BAA75265.1| (AB017508) ybxF homologue (identity of 59% to B. subtilis) [Bacillus halodurans] gi|10172740|dbj|BAB03847.1| (AP001507) ribosomal protein L7AE family [Bacillus halodurans], score 77, E-value 4.00E-14. (82 aa) |
| | RpoC | DNA-directed RNA polymerase beta subunit/160 kD subunit (split gene in archaea and Syn); DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1183 aa) |
| | RpoB | DNA-directed RNA polymerase beta subunit/140 kD subunit (split gene in Mjan, Mthe, Aful); DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1234 aa) |
| | RplL | Ribosomal protein L7/L12; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family. (125 aa) |
| | RplJ | Ribosomal protein L10; Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. Belongs to the universal ribosomal protein uL10 family. (177 aa) |
| | RplA | Ribosomal protein L1; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (231 aa) |
| | RplK | Ribosomal protein L11; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. (140 aa) |
| | NusG3 | Transcription antiterminator; Participates in transcription elongation, termination and antitermination. (174 aa) |
| | SecE | Preprotein translocase subunit SecE; Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. (66 aa) |
| | RpmG | Ribosomal protein L33; Best Blastp hit = gi|3914760|sp|O67756|RL33_AQUAE 50S RIBOSOMAL PROTEIN L33 gi|7441150|pir||H70465 ribosomal protein L33 - Aquifex aeolicus gi|2984181|gb|AAC07713.1| (AE000763) ribosomal protein L33 [Aquifex aeolicus], score 77.8, E-value 2.00E-14; Belongs to the bacterial ribosomal protein bL33 family. (49 aa) |
| | TufB2 | GTPases - translation elongation factors; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (400 aa) |
| | ProS | Prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (573 aa) |
| | FusA2 | Translation elongation and release factors (GTPases); Best Blastp hit = gi|2494254|sp|P74228|EFG2_SYNY3 ELONGATION FACTOR G 2 (EF-G 2) gi|7443433|pir||S75863 translation elongation factor EF-G.sll1098 - Synechocystis sp. (strain PCC 6803) gi|1653408|dbj|BAA18322.1| (D90913) elongation factor EF-G [Synechocystis sp.], score 543, E-value 1.00E-153. (700 aa) |
| | GreA | Transcription elongation factor; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (157 aa) |
| | Ppx1 | Best Blastp hit = gi|8134646|sp|Q9WZ56|PPAC_THEMA PROBABLE MANGANESE-DEPENDENT INORGANIC PYROPHOSPHATASE (PYROPHOSPHATE PHOSPHO-HYDROLASE) (PPASE), score 344, E-value 2.00E-93. (539 aa) |
| | ArgS | Arginyl-tRNA synthetase; Best Blastp hit = gi|10176433|dbj|BAB07527.1| (AP001520) arginyl-tRNA synthetase [Bacillus halodurans], score 575, E-value 1.00E-163. (562 aa) |
| | PyrG | CTP synthase (UTP-ammonia lyase); Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates. (537 aa) |
| | RplI | Ribosomal protein L9; Binds to the 23S rRNA. (147 aa) |
| | RpsR | Ribosomal protein S18; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (89 aa) |
| | RpsF | Ribosomal protein S6; Binds together with S18 to 16S ribosomal RNA. (95 aa) |
| | Jag | Predicted RNA-binding protein; Best Blastp hit = gi|5672643|dbj|BAA82681.1| (AB013492) 43%-identity [Bacillus halodurans] gi|10176688|dbj|BAB07782.1| (AP001520) spoIIIJ-associated protein [Bacillus halodurans], score 160, E-value 9.00E-39. (206 aa) |
