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| | rluA_2 | [J] COG0564 Pseudouridylate synthases, 23S RNA-specific. (237 aa) |
| | rlmL | Methyltransferase small domain protein; Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA. Belongs to the methyltransferase superfamily. RlmKL family. (245 aa) |
| | sigK | sigma70-ECF: RNA polymerase sigma factor, sigma-70 family protein; [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog. (184 aa) |
| | rlmF | Hypothetical protein; Specifically methylates the adenine in position 1618 of 23S rRNA. (320 aa) |
| | dus_2 | Dihydrouridine synthase family protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs. Belongs to the Dus family. DusC subfamily. (310 aa) |
| | trmU | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s(2)U34, the first step of tRNA-mnm(5)s(2)U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs; Belongs to the MnmA/TRMU family. (373 aa) |
| | ymfC | Pseudouridine synthase family protein; [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases; Belongs to the pseudouridine synthase RsuA family. (208 aa) |
| | glnS | glnS: glutamine--tRNA ligase; [J] COG0008 Glutamyl- and glutaminyl-tRNA synthetases; Belongs to the class-I aminoacyl-tRNA synthetase family. (555 aa) |
| | miaB | tRNA-i(6)A37 thiotransferase enzyme MiaB; Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6- (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. (474 aa) |
| | ybeY | Putative rRNA maturation factor YbeY; Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. (157 aa) |
| | leuS | leuS_bact: leucine--tRNA ligase; [J] COG0495 Leucyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (860 aa) |
| | rlmH | rRNA large subunit m3Psi methyltransferase RlmH; Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA; Belongs to the RNA methyltransferase RlmH family. (156 aa) |
| | gltX | glutamate--tRNA ligase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). (471 aa) |
| | tmcA | Acetyltransferase family protein; Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). (678 aa) |
| | CH54_1373 | Hypothetical protein. (112 aa) |
| | hisS | hisS: histidine--tRNA ligase; [J] COG0124 Histidyl-tRNA synthetase. (424 aa) |
| | pilF | type_IV_pilW: type IV pilus biogenesis/stability protein PilW; [NU] COG3063 Tfp pilus assembly protein PilF. (250 aa) |
| | rlmN | 23S rRNA (adenine(2503)-C(2))-methyltransferase; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity; Belongs to the radical SAM superfamily. RlmN family. (398 aa) |
| | trmJ | RNA methyltransferase, TrmH, group 1 family protein; Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA. (257 aa) |
| | tadA_2 | Cytidine and deoxycytidylate deaminase zinc-binding region family protein; Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2); Belongs to the cytidine and deoxycytidylate deaminase family. (199 aa) |
| | fdx_1 | 4Fe-4S dicluster domain protein; [C] COG1145 Ferredoxin. (86 aa) |
| | rnc | RNaseIII: ribonuclease III; [K] COG0571 dsRNA-specific ribonuclease. (226 aa) |
| | rpoE | rpoE_Sigma70: RNA polymerase sigma factor RpoE; [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog; Belongs to the sigma-70 factor family. ECF subfamily. (191 aa) |
| | yfiC | Methyltransferase small domain protein; Specifically methylates the adenine in position 37 of tRNA(1)(Val) (anticodon cmo5UAC). (219 aa) |
| | CH54_1528 | Hypothetical protein. (518 aa) |
| | rnhA | RNase H family protein; Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. (154 aa) |
| | rluD | Pseudouridine synthase, RluA family protein; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (325 aa) |
| | trmD | tRNA (guanine(37)-N(1))-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (246 aa) |
| | rimM | 16S rRNA processing protein RimM; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (182 aa) |
| | csrA | Carbon storage regulator; A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems. Usually binds in the 5'-UTR; binding at or near the Shine-Dalgarno sequence prevents ribosome-binding, repressing translation, binding elsewhere in the 5'-UTR can activate translation and/or stabilize the mRNA. Its function is antagonized by small RNA(s). (61 aa) |
| | alaS | alanine--tRNA ligase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain; Belongs to the class-II aminoacyl-tRNA synthetase family. (875 aa) |
| | rpoS | RNA polymerase sigma factor RpoS; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. (332 aa) |
| | truD | tRNA pseudouridine synthase, TruD family protein; Responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs; Belongs to the pseudouridine synthase TruD family. (349 aa) |
| | hrpB | DEAH_box_HrpB: ATP-dependent helicase HrpB; [L] COG1643 HrpA-like helicases. (841 aa) |
| | gluQ | Glutamyl-queuosine tRNA(Asp) synthetase; Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon; Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily. (302 aa) |
| | pcnB | poly(A) polymerase family protein; Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. (478 aa) |
| | rsmH | 16S rRNA (cytosine(1402)-N(4))-methyltransferase; Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. (320 aa) |
| | rluA_1 | [J] COG0564 Pseudouridylate synthases, 23S RNA-specific. (217 aa) |
| | rsmA | Ribosomal RNA small subunit methyltransferase A; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (272 aa) |
| | apaH | Diadenosine tetraphosphatase; Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP; Belongs to the Ap4A hydrolase family. (292 aa) |
| | ileS | isoleucine--tRNA ligase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (938 aa) |
| | rsmC | Methyltransferase small domain protein; Specifically methylates the guanine in position 1207 of 16S rRNA in the 30S particle; Belongs to the methyltransferase superfamily. RsmC family. (347 aa) |
| | rluF | Pseudouridine synthase family protein; [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases; Belongs to the pseudouridine synthase RsuA family. (290 aa) |
| | valS | valine--tRNA ligase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (965 aa) |
| | deaD | DEAD/DEAH box helicase family protein; DEAD-box RNA helicase involved in various cellular processes at low temperature, including ribosome biogenesis, mRNA degradation and translation initiation. (659 aa) |
| | pnp | Polyribonucleotide nucleotidyltransferase; Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction. (706 aa) |
| | truB | tRNA pseudouridine(55) synthase; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (314 aa) |
| | rbfA | Ribosome-binding factor A; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (136 aa) |
| | nusA | Transcription elongation factor; Participates in both transcription termination and antitermination. (495 aa) |
| | rrmJ | Ribosomal RNA large subunit methyltransferase J; Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit. (209 aa) |
| | yhbY | CRS1 / YhbY domain protein; [J] COG1534 Predicted RNA-binding protein containing KH domain, possibly ribosomal protein. (97 aa) |
| | greA | Transcription elongation factor GreA domain protein; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (158 aa) |
| | CH54_2024 | Single-strand binding family protein; [L] COG2965 Primosomal replication protein N. (106 aa) |
| | rlmB | RNA 2'-O ribose methyltransferase substrate binding family protein; Specifically methylates the ribose of guanosine 2251 in 23S rRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. RlmB subfamily. (246 aa) |
| | rnr | Ribonuclease R; 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs. Belongs to the RNR ribonuclease family. RNase R subfamily. (848 aa) |
| | miaA | tRNA dimethylallyltransferase; Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A); Belongs to the IPP transferase family. (313 aa) |
| | CH54_2042 | T6A_YjeE: tRNA threonylcarbamoyl adenosine modification protein YjeE; [R] COG0802 Predicted ATPase or kinase. (162 aa) |
| | queG | Epoxyqueuosine reductase; Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr); Belongs to the QueG family. (333 aa) |
| | b4162 | Exonuclease family protein; 3'-to-5' exoribonuclease specific for small oligoribonucleotides; Belongs to the oligoribonuclease family. (181 aa) |
| | b4155 | EF-P lysine aminoacylase GenX; With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P). Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of a conserved specific lysine residue in EF-P; Belongs to the class-II aminoacyl-tRNA synthetase family. EpmA subfamily. (325 aa) |
| | CH54_2112 | Conserved hypothetical protein; [S] COG4679 Phage-related protein. (119 aa) |
| | CH54_2123 | Conserved hypothetical protein; [S] COG4679 Phage-related protein. (114 aa) |
| | dnaB | dnaB: replicative DNA helicase; [L] COG0305 Replicative DNA helicase. (447 aa) |
| | CH54_2169 | Sulfurtransferase TusA family protein; [O] COG0425 Predicted redox protein, regulator of disulfide bond formation. (94 aa) |
| | rimN | Telomere recombination family protein; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. (195 aa) |
| | fmt | methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. (315 aa) |
| | rsmB | 16S rRNA (cytosine(967)-C(5))-methyltransferase; Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA. (429 aa) |
| | zntR | zntR: Zn(II)-responsive transcriptional regulator; [K] COG0789 Predicted transcriptional regulators. (141 aa) |
| | rpoA | DNA-directed RNA polymerase, alpha subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (330 aa) |
| | rpsK | 30S ribosomal protein S11; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (129 aa) |
| | dsrH | Sulfur relay protein TusB/DsrH; Part of a sulfur-relay system required for 2-thiolation of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. (95 aa) |
| | tusC | Sulfur relay protein TusC/DsrF; Part of a sulfur-relay system required for 2-thiolation of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. (124 aa) |
| | tusD | dsrE/DsrF-like family protein; Part of a sulfur-relay system required for 2-thiolation of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from TusA and transfers it in turn to TusE. (134 aa) |
| | rlmI | Met-10+ like-family protein; [R] COG1092 Predicted SAM-dependent methyltransferases. (394 aa) |
| | trpS | tryptophan--tRNA ligase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (346 aa) |
| | greB | Transcription elongation factor GreB; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length. (165 aa) |
| | rsmJ | SAM-dependent methyltransferase family protein; Specifically methylates the guanosine in position 1516 of 16S rRNA. (252 aa) |
| | rlmJ | Hypothetical protein; Specifically methylates the adenine in position 2030 of 23S rRNA. (280 aa) |
| | selA | L-seryl-tRNA(Sec) selenium transferase; Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis; Belongs to the SelA family. (462 aa) |
| | glyS | glyS: glycine--tRNA ligase, beta subunit; [J] COG0751 Glycyl-tRNA synthetase, beta subunit. (689 aa) |
| | glyQ | glyQ: glycine--tRNA ligase, alpha subunit; [J] COG0752 Glycyl-tRNA synthetase, alpha subunit. (304 aa) |
| | rnpA | Ribonuclease P protein component; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (119 aa) |
| | trmE | tRNA modification GTPase TrmE; Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. (454 aa) |
| | thrS | threonine--tRNA ligase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). (642 aa) |
| | rsmG | 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG; Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. (206 aa) |
| | sbcB | Exonuclease C-terminal family protein; [L] COG2925 Exonuclease I. (476 aa) |
| | gidA | tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA; NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34; Belongs to the MnmG family. (629 aa) |
| | pheS | pheS: phenylalanine--tRNA ligase, alpha subunit; [J] COG0016 Phenylalanyl-tRNA synthetase alpha subunit; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (327 aa) |
| | pheT | pheT_bact: phenylalanine--tRNA ligase, beta subunit; [J] COG0072 Phenylalanyl-tRNA synthetase beta subunit; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (795 aa) |
| | dtd | D-tyrosyl-tRNA(Tyr) deacylase; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. Belongs to the DTD family. (145 aa) |
| | spoU | spoU rRNA Methylase family protein; Catalyzes the 2'-O methylation of guanosine at position 18 in tRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (219 aa) |
| | rpoZ | DNA-directed RNA polymerase, omega subunit; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (91 aa) |
| | yicC | Conserved hypothetical protein; [S] COG1561 Uncharacterized stress-induced protein. (287 aa) |
| | rph | Ribonuclease PH; Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. (238 aa) |
| | tusE | Sulfur relay, TusE/DsrC/DsvC family protein; Part of a sulfur-relay system. (109 aa) |
| | trmL | tRNA (cytidine(34)-2'-O)-methyltransferase; Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. (172 aa) |
| | priA | Primosomal protein N; Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA; Belongs to the helicase family. PriA subfamily. (732 aa) |
| | trmA | tRNA (uracil(54)-C(5))-methyltransferase; Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA). (367 aa) |
| | rhlB | DEAD/DEAH box helicase family protein; DEAD-box RNA helicase involved in RNA degradation. Has RNA- dependent ATPase activity and unwinds double-stranded RNA. Belongs to the DEAD box helicase family. RhlB subfamily. (428 aa) |
| | rho | Transcription termination factor; Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. (419 aa) |
| | tusA | Sulfurtransferase TusA family protein; Sulfur carrier protein involved in sulfur trafficking in the cell. Part of a sulfur-relay system required for 2-thiolation during synthesis of 2-thiouridine of the modified wobble base 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with IscS and stimulates its cysteine desulfurase activity. Accepts an activated sulfur from IscS, which is then transferred to TusD, and thus determines the direction of sulfur flow from IscS to 2-thiouridine formation. Also appears to be involved in sulfur transfer for the biosynthesis of molybdopterin. (84 aa) |
| | rsmD | 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD; Specifically methylates the guanine in position 966 of 16S rRNA in the assembled 30S particle; Belongs to the methyltransferase superfamily. RsmD family. (217 aa) |
| | rpoH | Alternative sigma factor RpoH; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. (285 aa) |
| | metG | methionine--tRNA ligase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (676 aa) |
| | mttC | tatD related DNase family protein; 3'-5' exonuclease that prefers single-stranded DNA and RNA. May play a role in the H(2)O(2)-induced DNA damage repair. Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family. TatD subfamily. (260 aa) |
| | nusG | Transcription antiterminator; Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNA polymerase (RNAP), probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA transcriptional antitermination; Belongs to t [...] (181 aa) |
| | rplA | Ribosomal protein L1; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (234 aa) |
| | rpoB | DNA-directed RNA polymerase, beta subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1342 aa) |
| | rpoC | DNA-directed RNA polymerase, beta' subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1406 aa) |
| | nfi | Endonuclease V family protein; DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. (234 aa) |
| | dusA | tRNA dihydrouridine synthase A family protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs; Belongs to the Dus family. DusA subfamily. (343 aa) |
| | dnaB-2 | Replicative DNA helicase; Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins. Belongs to the helicase family. DnaB subfamily. (468 aa) |
| | dus_1 | TIM-barrel, nifR3 family protein; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines; Belongs to the dus family. (321 aa) |
| | yhdA | [T] COG2200 FOG: EAL domain. (639 aa) |
| | cafA | RNaseEG: ribonuclease, Rne/Rng family domain protein; [J] COG1530 Ribonucleases G and E. (489 aa) |
| | CH54_2983 | Ribonuclease family protein. (157 aa) |
| | selA-2 | Beta-eliminating lyase family protein; [E] COG1921 Selenocysteine synthase [seryl-tRNASer selenium transferase]. (370 aa) |
| | rpoN | RNA polymerase sigma-54 factor; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. (477 aa) |
| | rsmI | 16S rRNA (cytidine(1402)-2'-O)-methyltransferase; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. (299 aa) |
| | rnt | Ribonuclease T; Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis. (227 aa) |
| | rlmG | Methyltransferase small domain protein; Specifically methylates the guanine in position 1835 (m2G1835) of 23S rRNA. (395 aa) |
| | rpoD | RNA polymerase sigma factor RpoD; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. (612 aa) |
| | dnaG | DNA primase; RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. (582 aa) |
| | tsaD | tRNA threonylcarbamoyl adenosine modification protein TsaD; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction; Belongs to the KAE1 / TsaD family. (337 aa) |
| | cca | Poly A polymerase head domain protein; Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases. (441 aa) |
| | fliA_2 | fliA_WhiG: RNA polymerase sigma factor, FliA/WhiG family protein; [K] COG1191 DNA-directed RNA polymerase specialized sigma subunit; Belongs to the sigma-70 factor family. (228 aa) |
| | tyrS | tyrosine--tRNA ligase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (424 aa) |
| | CH54_3308 | Conserved hypothetical protein; [S] COG4679 Phage-related protein. (107 aa) |
| | dnaB-3 | dnaB: replicative DNA helicase; [L] COG0305 Replicative DNA helicase. (447 aa) |
| | trmB | tRNA (guanine-N(7)-)-methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. (265 aa) |
| | yqgF | Hypothetical protein; Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA; Belongs to the YqgF HJR family. (140 aa) |
| | rsmE | RNA methyltransferase, RsmE family protein; Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit. (243 aa) |
| | ygfZ | Aminomethyltransferase folate-binding domain protein; Folate-binding protein involved in regulating the level of ATP-DnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication; Belongs to the tRNA-modifying YgfZ family. (330 aa) |
| | lysS | lysS_bact: lysine--tRNA ligase; [J] COG1190 Lysyl-tRNA synthetase (class II); Belongs to the class-II aminoacyl-tRNA synthetase family. (505 aa) |
| | CH54_3433 | Plasmid stabilization system family protein; [JD] COG2026 Cytotoxic translational repressor of toxin-antitoxin stability system. (86 aa) |
| | CH54_3438 | Zinc-binding domain of primase-helicase family protein; [S] COG4643 Uncharacterized protein conserved in bacteria. (764 aa) |
| | rppH_1 | NUDIX domain protein; Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage; Belongs to the Nudix hydrolase family. RppH subfamily. (175 aa) |
| | thiF_1 | thiF family protein; [H] COG1179 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1. (272 aa) |
| | rlmM | ftsJ-like methyltransferase family protein; Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. RlmM subfamily. (368 aa) |
| | truC | [J] COG0564 Pseudouridylate synthases, 23S RNA-specific. (257 aa) |
| | rnhB | Ribonuclease HII family protein; Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. (198 aa) |
| | ttcA | PP-loop family protein; Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system. (313 aa) |
| | tilS | tRNA(Ile)-lysidine synthetase; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Belongs to the tRNA(Ile)-lysidine synthase family. (461 aa) |
| | proS | proline--tRNA ligase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacy [...] (572 aa) |
| | CH54_3563 | DTW domain protein; [S] COG3148 Uncharacterized conserved protein. (252 aa) |
| | CH54_3565 | RNA 2'-O ribose methyltransferase substrate binding family protein; [J] COG0566 rRNA methylases; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (363 aa) |
| | CH54_3620 | Indigoidine synthase A like family protein; [Q] COG2313 Uncharacterized enzyme involved in pigment biosynthesis. (311 aa) |
| | hrpA | DEAH_box_HrpA: RNA helicase HrpA; [L] COG1643 HrpA-like helicases. (1295 aa) |
| | queA | tRNA ribosyltransferase-isomerase; Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). (356 aa) |
| | tgt | tRNA-guanine transglycosylase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, - Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the [...] (378 aa) |
| | CH54_366 | Conserved hypothetical protein; [S] COG4679 Phage-related protein. (109 aa) |
| | nusB | Transcription antitermination factor NusB; Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. (138 aa) |
| | thiI | tRNA sulfurtransferase ThiI; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. (483 aa) |
| | bolA | bolA-like family protein; [T] COG0271 Stress-induced morphogen (activity unknown); Belongs to the BolA/IbaG family. (106 aa) |
| | ychN | dsrE/DsrF-like family protein; [P] COG1553 Uncharacterized conserved protein involved in intracellular sulfur reduction. (121 aa) |
| | priC | Primosomal replication priB and priC family protein; [L] COG3923 Primosomal replication protein N''. (178 aa) |
| | ybaK | aminoacyl-tRNA editing domain protein; [S] COG2606 Uncharacterized conserved protein; Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily. (159 aa) |
| | cysS | cysS: cysteine--tRNA ligase; [J] COG0215 Cysteinyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (462 aa) |
| | CH54_3804 | Hypothetical protein. (107 aa) |
| | mnmC | tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC; Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34; In the C-terminal section; belongs to the DAO family. (687 aa) |
| | truA | tRNA pseudouridine(38-40) synthase; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (280 aa) |
| | rsuA | Pseudouridine synthase family protein; [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases; Belongs to the pseudouridine synthase RsuA family. (235 aa) |
| | rumA | 23S rRNA (uracil-5-)-methyltransferase RumA; Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily. (376 aa) |
| | serS | serine--tRNA ligase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (430 aa) |
| | smtA2 | Methyltransferase small domain protein; Catalyzes the methylation of 5-carboxymethoxyuridine (cmo5U) to form 5-methoxycarbonylmethoxyuridine (mcmo5U) at position 34 in tRNAs; Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoM family. (261 aa) |
| | asnS | asnS: asparagine--tRNA ligase; [J] COG0017 Aspartyl/asparaginyl-tRNA synthetases. (466 aa) |
| | CH54_415 | Acetyltransferase family protein; [KR] COG0454 Histone acetyltransferase HPA2 and related acetyltransferases. (298 aa) |
| | rnb | Exoribonuclease II; Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction. (644 aa) |
| | rluB | Pseudouridine synthase family protein; [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases; Belongs to the pseudouridine synthase RsuA family. (349 aa) |
| | yciO | tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family; [J] COG0009 Putative translation factor (SUA5); Belongs to the SUA5 family. (206 aa) |
| | CH54_524 | PHP domain protein; [R] COG0613 Predicted metal-dependent phosphoesterases (PHP family). (313 aa) |
| | rnd | Ribonuclease D; Exonuclease involved in the 3' processing of various precursor tRNAs. Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides; Belongs to the RNase D family. (373 aa) |
| | yeaZ | T6A_YeaZ: tRNA threonylcarbamoyl adenosine modification protein YeaZ; [O] COG1214 Inactive homolog of metal-dependent proteases, putative molecular chaperone. (232 aa) |
| | CH54_61 | Rhodanese-like domain protein; [R] COG1054 Predicted sulfurtransferase; Belongs to the UPF0176 family. (354 aa) |
| | aspS | aspartate--tRNA ligase; Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (598 aa) |
| | cmoA | tRNA (cmo5U34)-methyltransferase; Catalyzes the conversion of S-adenosyl-L-methionine (SAM) to carboxy-S-adenosyl-L-methionine (Cx-SAM). (284 aa) |
| | cmoB | tRNA (mo5U34)-methyltransferase; Catalyzes carboxymethyl transfer from carboxy-S-adenosyl-L- methionine (Cx-SAM) to 5-hydroxyuridine (ho5U) to form 5- carboxymethoxyuridine (cmo5U) at position 34 in tRNAs. (323 aa) |
| | argS | argS: arginine--tRNA ligase; [J] COG0018 Arginyl-tRNA synthetase. (576 aa) |
| | CH54_699 | Threonyl and Alanyl tRNA synthetase second additional domain protein; [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain. (209 aa) |
| | rne | Ribonuclease, Rne/Rng family domain protein; Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs. Belongs to the RNase E/G family. RNase E subfamily. (1152 aa) |
| | rluC | Pseudouridine synthase, RluA family protein; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (319 aa) |
| | fliA_1 | RNA polymerase sigma factor, FliA/WhiG family protein; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor controls the expression of flagella-related genes; Belongs to the sigma-70 factor family. FliA subfamily. (240 aa) |
