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| | rpmH | COG0230 [J] Ribosomal protein L34; Belongs to the bacterial ribosomal protein bL34 family. (44 aa) |
| | rpsF | SSU ribosomal protein S6P; Binds together with S18 to 16S ribosomal RNA. (96 aa) |
| | rpsR | SSU ribosomal protein S18P; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (78 aa) |
| | rlmH | Conserved hypothetical protein; Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA; Belongs to the RNA methyltransferase RlmH family. (159 aa) |
| | greA | Transcription elongation factor; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (157 aa) |
| | mnmE | tRNA (5-carboxymethylaminomethyl-2-thiouridylate) synthase; Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. (464 aa) |
| | gidA | Putative tRNA (5-carboxymethylaminomethyl-2-thiouridylate) synthase subunit; NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34; Belongs to the MnmG family. (637 aa) |
| | LSL_0126 | CAAX amino terminal protease family; COG1266 [R] Predicted metal-dependent membrane protease. (287 aa) |
| | lplA | COG0095 [H] Lipoate-protein ligase A. (339 aa) |
| | serS | Seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (425 aa) |
| | rpoB | DNA-directed RNA polymerase beta chain; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1199 aa) |
| | rpoC | DNA-directed RNA polymerase beta' chain; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1221 aa) |
| | rpsL | SSU ribosomal protein S12P; With S4 and S5 plays an important role in translational accuracy. (137 aa) |
| | rpsG | SSU ribosomal protein S7P; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | efg | Protein Translation Elongation Factor G; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF- [...] (697 aa) |
| | trpS | Tryptophanyl-tRNA synthetase; Catalyzes the attachment of tryptophan to tRNA(Trp). Belongs to the class-I aminoacyl-tRNA synthetase family. (339 aa) |
| | metG | Methionyl-tRNA synthetase / Protein secretion chaperonin CsaA; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation; Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. (675 aa) |
| | rnmV1 | Ribonuclease M5; Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step; Belongs to the ribonuclease M5 family. (185 aa) |
| | ksgA | Dimethyladenosine transferase; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (296 aa) |
| | birA | Biotin operon repressor / Biotin--[acetyl-CoA-carboxylase] synthetase; Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor; Belongs to the biotin--protein ligase family. (321 aa) |
| | rpoE | DNA-directed RNA polymerase delta chain; Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling; Belongs to the RpoE family. (196 aa) |
| | rho | Transcription termination factor; Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. (427 aa) |
| | rpmE | COG0254 [J] Ribosomal protein L31. (82 aa) |
| | LSL_0367 | Sigma-70 factor family protein. (192 aa) |
| | trmA | tRNA (Uracil-5-) -methyltransferase; COG2265 [J] SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. (459 aa) |
| | prfC | Bacterial Peptide Chain Release Factor 3; Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP. Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily. (525 aa) |
| | leuS | COG0495 [J] Leucyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (805 aa) |
| | rsuA | Ribosomal small subunit pseudouridine synthase A; COG1187 [J] 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases; Belongs to the pseudouridine synthase RsuA family. (240 aa) |
| | trmB | tRNA (m(7)G46) methyltransferase; Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. (212 aa) |
| | thrS | Threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (649 aa) |
| | infC | Bacterial Protein Translation Initiation Factor 3 (IF-3); IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (174 aa) |
| | rpmI | COG0291 [J] Ribosomal protein L35; Belongs to the bacterial ribosomal protein bL35 family. (66 aa) |
| | rplT | LSU ribosomal protein L20P; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit. (120 aa) |
| | tmcAL | Conserved hypothetical protein; Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of elongator tRNA(Met), using acetate and ATP as substrates. First activates an acetate ion to form acetyladenylate (Ac- AMP) and then transfers the acetyl group to tRNA to form ac(4)C34. (380 aa) |
| | rpmF | COG0333 [J] Ribosomal protein L32; Belongs to the bacterial ribosomal protein bL32 family. (58 aa) |
| | rpsB | COG0052 [J] Ribosomal protein S2; Belongs to the universal ribosomal protein uS2 family. (265 aa) |
| | tsf | Protein Translation Elongation Factor Ts; Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Belongs to the EF-Ts family. (291 aa) |
| | efp | Protein Translation Elongation Factor P; Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. (185 aa) |
| | nusB | N utilization substance protein B; Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. (131 aa) |
| | rpmG | COG0267 [J] Ribosomal protein L33; Belongs to the bacterial ribosomal protein bL33 family. (49 aa) |
| | miaA | tRNA delta(2)-isopentenylpyrophosphate transferase; Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A); Belongs to the IPP transferase family. (307 aa) |
| | frr | Ribosome Recycling Factor, RRF; Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another; Belongs to the RRF family. (187 aa) |
| | proS | Prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (571 aa) |
| | nusA | N utilization substance protein A; Participates in both transcription termination and antitermination. (375 aa) |
| | infB | Bacterial Protein Translation Initiation Factor 2 (IF-2); One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (737 aa) |
| | rbfA | Ribosome-binding factor A; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (119 aa) |
| | truB | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (302 aa) |
| | lepA | GTP-binding translation elongation factor; Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner. (609 aa) |
| | prfA | Bacterial Peptide Chain Release Factor 1; Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. (360 aa) |
| | LSL_0592 | Sua5/YciO/YrdC/YwlC family protein; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. (336 aa) |
| | rpoZ | DNA-directed RNA polymerase omega chain; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (65 aa) |
| | fmt | Methionyl-tRNA formyltransferase; Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus; Belongs to the Fmt family. (318 aa) |
| | LSL_0615 | 16S rRNA m(5)C 967 methyltransferase; Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA. (445 aa) |
| | rpmB | COG0227 [J] Ribosomal protein L28; Belongs to the bacterial ribosomal protein bL28 family. (61 aa) |
| | rnc | Ribonuclease III; Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. (232 aa) |
| | rpsP | COG0228 [J] Ribosomal protein S16; Belongs to the bacterial ribosomal protein bS16 family. (91 aa) |
| | rimM | 16S rRNA processing protein; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (168 aa) |
| | trmD | tRNA (Guanine-N(1)-)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (250 aa) |
| | rplS | LSU ribosomal protein L19P; This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. (118 aa) |
| | rpsT | SSU ribosomal protein S20P; Binds directly to 16S ribosomal RNA. (84 aa) |
| | rpsO | SSU ribosomal protein S15P; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA. (89 aa) |
| | rnj | Metallo-beta-lactamase superfamily protein; An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay. (565 aa) |
| | tufB | Protein Translation Elongation Factor Tu; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (395 aa) |
| | rnj-2 | Metallo-beta-lactamase superfamily protein; An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay. (558 aa) |
| | def-2 | Peptide deformylase; Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. (186 aa) |
| | typA | GTP-binding protein; COG1217 [T] Predicted membrane GTPase involved in stress response. (613 aa) |
| | LSL_0660 | Methyltransferase; COG0742 [L] N6-adenine-specific methylase. (186 aa) |
| | argS | COG0018 [J] Arginyl-tRNA synthetase. (563 aa) |
| | asnS | COG0017 [J] Aspartyl/asparaginyl-tRNA synthetases. (433 aa) |
| | rpsU | COG0828 [J] Ribosomal protein S21; Belongs to the bacterial ribosomal protein bS21 family. (61 aa) |
| | pcnB | Poly(A) polymerase / tRNA nucleotidyltransferase; Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. (404 aa) |
| | trmFO | Glucose inhibited division protein A; Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs; Belongs to the MnmG family. TrmFO subfamily. (436 aa) |
| | spoU | 23S rRNA methyltransferase; COG0566 [J] rRNA methylases; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (253 aa) |
| | pheS | COG0016 [J] Phenylalanyl-tRNA synthetase alpha subunit; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (348 aa) |
| | pheT | COG0072 [J] Phenylalanyl-tRNA synthetase beta subunit; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (805 aa) |
| | greA-2 | Transcription elongation factor; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. (156 aa) |
| | LSL_0821 | Conserved hypothetical protein; Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit. (243 aa) |
| | rluD | Ribosomal large subunit pseudouridine synthase D; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (298 aa) |
| | pyrR | Uracil phosphoribosyltransferase / Pyrimidine operon regulatory protein pyrR; Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes. Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily. (178 aa) |
| | LSL_0839 | Conserved hypothetical protein; COG0116 [L] Predicted N6-adenine-specific DNA methylase; Belongs to the methyltransferase superfamily. (376 aa) |
| | LSL_0846 | Conserved hypothetical protein; COG2384 [R] Predicted SAM-dependent methyltransferase. (232 aa) |
| | hisS | COG0124 [J] Histidyl-tRNA synthetase. (431 aa) |
| | aspS | Aspartyl-tRNA synthetase; Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (590 aa) |
| | trmU | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. (375 aa) |
| | LSL_0873 | Fibronectin-binding protein / Fibrinogen-binding protein; COG1293 [K] Predicted RNA-binding protein homologous to eukaryotic snRNP. (562 aa) |
| | lepB | COG0681 [U] Signal peptidase I; Belongs to the peptidase S26 family. (195 aa) |
| | ybeY | Conserved hypothetical protein; Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. (159 aa) |
| | rpoD | RNA polymerase sigma factor; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. (372 aa) |
| | glyS | COG0751 [J] Glycyl-tRNA synthetase, beta subunit. (693 aa) |
| | glyQ | COG0752 [J] Glycyl-tRNA synthetase, alpha subunit. (305 aa) |
| | rluB | Ribosomal large subunit pseudouridine synthase B; COG1187 [J] 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases; Belongs to the pseudouridine synthase RsuA family. (242 aa) |
| | rpmA | COG0211 [J] Ribosomal protein L27; Belongs to the bacterial ribosomal protein bL27 family. (93 aa) |
| | rplU | LSU ribosomal protein L21P; This protein binds to 23S rRNA in the presence of protein L20; Belongs to the bacterial ribosomal protein bL21 family. (102 aa) |
| | LSL_0962 | Hypothetical protein. (173 aa) |
| | LSL_0999 | Putative 23S rRNA m(5)C methyltransferase; COG0144 [J] tRNA and rRNA cytosine-C5-methylases. (456 aa) |
| | ileS | Isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (931 aa) |
| | mraW | S-adenosyl-methyltransferase; Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. (314 aa) |
| | valS | Valyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (886 aa) |
| | thiI | Thiamine biosynthesis protein; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. (405 aa) |
| | rpsD | SSU ribosomal protein S4P; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. (201 aa) |
| | rnz | Ribonuclease Z; Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA; Belongs to the RNase Z family. (317 aa) |
| | LSL_1109 | Endonuclease involved in recombination; Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA; Belongs to the YqgF nuclease family. (143 aa) |
| | alaS | Alanyl-tRNA synthetase; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain. (880 aa) |
| | tgt | Queuine tRNA-ribosyltransferase; Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, - Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form t [...] (381 aa) |
| | LSL_1136 | 23S rRNA methyltransferase; Could methylate the ribose at the nucleotide 34 wobble position in tRNA; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily. (170 aa) |
| | LSL_1138 | Transcriptional regulator; COG0011 [S] Uncharacterized conserved protein; Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily. (171 aa) |
| | LSL_1150 | ATP/GTP hydrolase; COG0802 [R] Predicted ATPase or kinase. (150 aa) |
| | smpB | SsrA-binding protein; Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to [...] (155 aa) |
| | prfB | Bacterial Peptide Chain Release Factor 2; Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (355 aa) |
| | LSL_1213 | Membrane-bound protease, CAAX family; COG1266 [R] Predicted metal-dependent membrane protease. (213 aa) |
| | gcp | O-sialoglycoprotein endopeptidase; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction; Belongs to the KAE1 / TsaD family. (343 aa) |
| | rimI | COG0456 [R] Acetyltransferases. (168 aa) |
| | LSL_1218 | Glycoprotein endopeptidase; COG1214 [O] Inactive homolog of metal-dependent proteases, putative molecular chaperone. (243 aa) |
| | rsmI | Tetrapyrrole (Corrin/Porphyrin) methylase family protein; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. (293 aa) |
| | tadA | Cytosine/adenosine deaminase; Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2); Belongs to the cytidine and deoxycytidylate deaminase family. (166 aa) |
| | rplL | LSU ribosomal protein L12P; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family. (122 aa) |
| | rplJ | LSU ribosomal protein L10P; Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. Belongs to the universal ribosomal protein uL10 family. (167 aa) |
| | rplA | LSU ribosomal protein L1P; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (230 aa) |
| | rplK | LSU ribosomal protein L11P; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. (141 aa) |
| | nusG | Transcription antitermination protein; Participates in transcription elongation, termination and antitermination. (186 aa) |
| | rpmG-2 | COG0267 [J] Ribosomal protein L33; Belongs to the bacterial ribosomal protein bL33 family. (50 aa) |
| | spoU-2 | TRNA/rRNA methyltransferase; COG0566 [J] rRNA methylases; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. (248 aa) |
| | mrnC | Conserved hypothetical protein; Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors; Belongs to the MrnC RNase family. (135 aa) |
| | cysS | COG0215 [J] Cysteinyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (470 aa) |
| | glnS | Glutamyl-tRNA synthetase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu); Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. (495 aa) |
| | rluD-2 | Ribosomal large subunit pseudouridine synthase D; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (295 aa) |
| | trmA-2 | tRNA (Uracil-5-) -methyltransferase; COG2265 [J] SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. (459 aa) |
| | gatB | Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln); Belongs to the GatB/GatE family. GatB subfamily. (477 aa) |
| | gatA | Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A; Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). (487 aa) |
| | gatC | Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln); Belongs to the GatC family. (99 aa) |
| | lysS | COG1190 [J] Lysyl-tRNA synthetase (class II); Belongs to the class-II aminoacyl-tRNA synthetase family. (495 aa) |
| | LSL_1352 | tRNA-dihydrouridine synthase; Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines; Belongs to the dus family. (330 aa) |
| | tilS | tRNA(Ile)-lysidine synthase TilS; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. (452 aa) |
| | pth | Peptidyl-tRNA hydrolase; The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Belongs to the PTH family. (185 aa) |
| | tyrS | Tyrosyl-tRNA synthetase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (416 aa) |
| | rnmV2 | Ribonuclease M5; Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step; Belongs to the ribonuclease M5 family. (189 aa) |
| | rpsI | COG0103 [J] Ribosomal protein S9; Belongs to the universal ribosomal protein uS9 family. (130 aa) |
| | rplM | LSU ribosomal protein L13P; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (147 aa) |
| | truA | tRNA pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (256 aa) |
| | rplQ | COG0203 [J] Ribosomal protein L17. (127 aa) |
| | rpoA | DNA-directed RNA polymerase alpha chain; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (314 aa) |
| | rpsK | SSU ribosomal protein S11P; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (128 aa) |
| | rpsM | SSU ribosomal protein S13P; Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. Belongs to the universal ribosomal protein uS13 family. (121 aa) |
| | rpmJ | COG0257 [J] Ribosomal protein L36; Belongs to the bacterial ribosomal protein bL36 family. (37 aa) |
| | infA | Bacterial Protein Translation Initiation Factor 1 (IF-1); One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (72 aa) |
| | rplO | LSU ribosomal protein L15P; Binds to the 23S rRNA; Belongs to the universal ribosomal protein uL15 family. (144 aa) |
| | rpmD | COG1841 [J] Ribosomal protein L30/L7E. (60 aa) |
| | rpsE | SSU ribosomal protein S5P; With S4 and S12 plays an important role in translational accuracy; Belongs to the universal ribosomal protein uS5 family. (166 aa) |
| | rplR | LSU ribosomal protein L18P; This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. (118 aa) |
| | rplF | LSU ribosomal protein L6P; This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center; Belongs to the universal ribosomal protein uL6 family. (178 aa) |
| | rpsH | SSU ribosomal protein S8P; One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; Belongs to the universal ribosomal protein uS8 family. (132 aa) |
| | rpsN | SSU ribosomal protein S14P; Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. (61 aa) |
| | rplE | LSU ribosomal protein L5P; This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. (180 aa) |
| | rplX | LSU ribosomal protein L24P; One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (101 aa) |
| | rplN | LSU ribosomal protein L14P; Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome; Belongs to the universal ribosomal protein uL14 family. (122 aa) |
| | rpsQ | SSU ribosomal protein S17P; One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. (88 aa) |
| | rpmC | COG0255 [J] Ribosomal protein L29; Belongs to the universal ribosomal protein uL29 family. (64 aa) |
| | rplP | LSU ribosomal protein L16P; Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs; Belongs to the universal ribosomal protein uL16 family. (144 aa) |
| | rpsC | SSU ribosomal protein S3P; Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation; Belongs to the universal ribosomal protein uS3 family. (218 aa) |
| | rplV | LSU ribosomal protein L22P; This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (115 aa) |
| | rpsS | SSU ribosomal protein S19P; Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (93 aa) |
| | rplB | LSU ribosomal protein L2P; One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Belongs to the universal ribosomal protein uL2 family. (277 aa) |
| | rplW | LSU ribosomal protein L23P; One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome; Belongs to the universal ribosomal protein uL23 family. (94 aa) |
| | rplD | LSU ribosomal protein L1E; One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. (207 aa) |
| | rplC | LSU ribosomal protein L3P; One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit; Belongs to the universal ribosomal protein uL3 family. (207 aa) |
| | rpsJ | SSU ribosomal protein S10P; Involved in the binding of tRNA to the ribosomes. Belongs to the universal ribosomal protein uS10 family. (102 aa) |
| | rluD-3 | Ribosomal large subunit pseudouridine synthase D; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (304 aa) |
| | rluD-4 | Ribosomal large subunit pseudouridine synthase D; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (296 aa) |
| | LSL_1575 | Hypothetical membrane associated protein; COG1266 [R] Predicted metal-dependent membrane protease. (213 aa) |
| | gidB | Methyltransferase; Specifically methylates the N7 position of a guanine in 16S rRNA; Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family. (241 aa) |
| | efp-2 | Protein Translation Elongation Factor P; Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. (185 aa) |
| | LSL_1720 | Hypothetical membrane spanning protein. (244 aa) |
| | rplI | LSU ribosomal protein L9P; Binds to the 23S rRNA. (149 aa) |
| | rnpA | Ribonuclease P protein component; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (113 aa) |
