(7 nodes) (2018) Newly Identified Essential Amino Acids Affecting Chlorella ellipsoidea DGAT1 Function Revealed by Site-Directed Mutagenesis. network (Arabidopsis thaliana)

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	GAL1	Galactokinase; Sugar-1-kinase with a very high substrate specificity for the alpha-anomeric configuration of D-galacose (D-Gal). Converts also efficiently 2-deoxy-D-Gal to 2-deoxy-D-al-1-phosphate. Belongs to the GHMP kinase family. GalK subfamily. (496 aa)
	DGAT3	Diacylglycerol O-acyltransferase 3; Involved in triacylglycerol (TAG) biosynthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. May preferentially use linolenoyl-CoA as substrate and to a lesser extent linoleoyl-CoA. May contribute to the active recycling of linoleate and linolenate into TAG when seed oil breakdown is blocked. (360 aa)
	DGAT1	Diacylglycerol O-acyltransferase 1; Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen. (520 aa)
	ASAT1	Acyl-CoA--sterol O-acyltransferase 1; Involved in the esterification of cycloartenol. Not implicated in the formation of sterol esters in flowers or during seed maturation. Has a substrate preference toward saturated fatty acyl donors (16:0 > 18:0 > 16:1 > 18:1). Does not require triacyglycerols (TAGs) as a fatty acyl donor, and is unable to acylate diacylglycerol to produce TAG. (345 aa)
	AAT1	Acetyl-CoA acetyltransferase, cytosolic 1. (403 aa)
	WSD1	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family. (481 aa)
	DGAT2	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA. (314 aa)

Your Current Organism:

Arabidopsis thaliana

NCBI taxonomy Id: 3702
Other names: A. thaliana, Arabidopsis thaliana (L.) Heynh., mouse-ear cress, thale cress, thale-cress

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Browse interactions in tabular form:

node1	node2	node1 accession	node2 accession	node1 annotation	node2 annotation	score
ASAT1	WSD1	Q9SV07	Q93ZR6	Acyl-CoA--sterol O-acyltransferase 1; Involved in the esterification of cycloartenol. Not implicated in the formation of sterol esters in flowers or during seed maturation. Has a substrate preference toward saturated fatty acyl donors (16:0 > 18:0 > 16:1 > 18:1). Does not require triacyglycerols (TAGs) as a fatty acyl donor, and is unable to acylate diacylglycerol to produce TAG.	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	0.441
DGAT1	DGAT2	Q9SLD2	Q9ASU1	Diacylglycerol O-acyltransferase 1; Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	0.999
DGAT1	DGAT3	Q9SLD2	Q9C5W0	Diacylglycerol O-acyltransferase 1; Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.	Diacylglycerol O-acyltransferase 3; Involved in triacylglycerol (TAG) biosynthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. May preferentially use linolenoyl-CoA as substrate and to a lesser extent linoleoyl-CoA. May contribute to the active recycling of linoleate and linolenate into TAG when seed oil breakdown is blocked.	0.996
DGAT1	WSD1	Q9SLD2	Q93ZR6	Diacylglycerol O-acyltransferase 1; Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	0.920
DGAT2	DGAT1	Q9ASU1	Q9SLD2	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	Diacylglycerol O-acyltransferase 1; Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.	0.999
DGAT2	DGAT3	Q9ASU1	Q9C5W0	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	Diacylglycerol O-acyltransferase 3; Involved in triacylglycerol (TAG) biosynthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. May preferentially use linolenoyl-CoA as substrate and to a lesser extent linoleoyl-CoA. May contribute to the active recycling of linoleate and linolenate into TAG when seed oil breakdown is blocked.	0.995
DGAT2	GAL1	Q9ASU1	Q9SEE5	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	Galactokinase; Sugar-1-kinase with a very high substrate specificity for the alpha-anomeric configuration of D-galacose (D-Gal). Converts also efficiently 2-deoxy-D-Gal to 2-deoxy-D-al-1-phosphate. Belongs to the GHMP kinase family. GalK subfamily.	0.498
DGAT2	WSD1	Q9ASU1	Q93ZR6	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	0.928
DGAT3	DGAT1	Q9C5W0	Q9SLD2	Diacylglycerol O-acyltransferase 3; Involved in triacylglycerol (TAG) biosynthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. May preferentially use linolenoyl-CoA as substrate and to a lesser extent linoleoyl-CoA. May contribute to the active recycling of linoleate and linolenate into TAG when seed oil breakdown is blocked.	Diacylglycerol O-acyltransferase 1; Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.	0.996
DGAT3	DGAT2	Q9C5W0	Q9ASU1	Diacylglycerol O-acyltransferase 3; Involved in triacylglycerol (TAG) biosynthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. May preferentially use linolenoyl-CoA as substrate and to a lesser extent linoleoyl-CoA. May contribute to the active recycling of linoleate and linolenate into TAG when seed oil breakdown is blocked.	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	0.995
DGAT3	WSD1	Q9C5W0	Q93ZR6	Diacylglycerol O-acyltransferase 3; Involved in triacylglycerol (TAG) biosynthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. May preferentially use linolenoyl-CoA as substrate and to a lesser extent linoleoyl-CoA. May contribute to the active recycling of linoleate and linolenate into TAG when seed oil breakdown is blocked.	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	0.964
GAL1	DGAT2	Q9SEE5	Q9ASU1	Galactokinase; Sugar-1-kinase with a very high substrate specificity for the alpha-anomeric configuration of D-galacose (D-Gal). Converts also efficiently 2-deoxy-D-Gal to 2-deoxy-D-al-1-phosphate. Belongs to the GHMP kinase family. GalK subfamily.	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	0.498
WSD1	ASAT1	Q93ZR6	Q9SV07	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	Acyl-CoA--sterol O-acyltransferase 1; Involved in the esterification of cycloartenol. Not implicated in the formation of sterol esters in flowers or during seed maturation. Has a substrate preference toward saturated fatty acyl donors (16:0 > 18:0 > 16:1 > 18:1). Does not require triacyglycerols (TAGs) as a fatty acyl donor, and is unable to acylate diacylglycerol to produce TAG.	0.441
WSD1	DGAT1	Q93ZR6	Q9SLD2	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	Diacylglycerol O-acyltransferase 1; Major contributor to triacylglycerol (TAG) synthesis and oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Can use oleoyl-CoA and linoleoyl-CoA as substrates. Has substrate preference for oleoyl-CoA compared to linoleoyl-CoA. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen.	0.920
WSD1	DGAT2	Q93ZR6	Q9ASU1	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	Diacylglycerol O-acyltransferase 2; Involved in triacylglycerol (TAG) synthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use oleoyl-CoA, linoleoyl-CoA and linolenoyl-CoA as substrates. Has substrate preference for linolenoyl-CoA or oleoyl-CoA compared to linoleoyl-CoA.	0.928
WSD1	DGAT3	Q93ZR6	Q9C5W0	O-acyltransferase WSD1; Bifunctional wax ester synthase/diacylglycerol acyltransferase. Involved in cuticular wax biosynthesis. In the N-terminal section; belongs to the long-chain O- acyltransferase family.	Diacylglycerol O-acyltransferase 3; Involved in triacylglycerol (TAG) biosynthesis. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. May preferentially use linolenoyl-CoA as substrate and to a lesser extent linoleoyl-CoA. May contribute to the active recycling of linoleate and linolenate into TAG when seed oil breakdown is blocked.	0.964