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| | phnL | ABC transporter, ATPase component; Paralog group: ATP_00013, ATP_00101, ATP_00485; Belongs to the ABC transporter superfamily. Macrolide exporter (TC 3.A.1.122) family. (549 aa) |
| | nfo | Endonuclease IV; Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin. (286 aa) |
| | srmB | Superfamily II DNA and RNA helicase; Paralog group: ATP_00028, ATP_00470. (398 aa) |
| | nusB | N utilization substance protein, transcription termination factor; Paralog group: ATP_00029, ATP_00469. (142 aa) |
| | sodA | Superoxide dismutase; Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Belongs to the iron/manganese superoxide dismutase family. (204 aa) |
| | adk | Adenylate kinase; Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism; Belongs to the adenylate kinase family. (224 aa) |
| | ATP_00039 | AAA+ ATPase. (467 aa) |
| | pepP | Xaa-Pro aminopeptidase. (420 aa) |
| | dnaX | DNA polymerase III, tau subunit; DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. (580 aa) |
| | recR | Recombinational DNA repair protein R; May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO. (206 aa) |
| | rnc | DsRNA-specific ribonuclease; Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. (225 aa) |
| | recG | ATP-dependent RecG-like helicase. (626 aa) |
| | dnaC | ATP-binding loader protein for DnaB. (266 aa) |
| | polA | DNA polymerase I (3'-5' exonuclease and polymerase domain. (295 aa) |
| | ffh | Signal recognition particle. (456 aa) |
| | ftsY | Cell divison protein FtsY homolog. (324 aa) |
| | topA | DNA topoisomerase I; Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supe [...] (599 aa) |
| | ATP_00076 | Ribosome biogenisis GTP-binding protein YlqF/GTPase; Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity; Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily. (289 aa) |
| | engC | GTPase EngC; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit; Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily. (304 aa) |
| | pheT | Phenylalanyl-tRNA synthetase beta chain; Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. (803 aa) |
| | pheS | Phenylalanyl-tRNA synthetase alpha chain; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. (341 aa) |
| | polC1 | DNA polymerase III, alpha subunit; Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity. (1522 aa) |
| | ATP_00087 | Weakly similar to IcmE protein. (1190 aa) |
| | trpS | Tryptophanyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (343 aa) |
| | lysU | Lysyl-tRNA synthetase; Belongs to the class-II aminoacyl-tRNA synthetase family. (497 aa) |
| | dnaE | DNA polymerase III, alpha subunit. (920 aa) |
| | prfB | Peptide chain release factor 2 (RF-2); Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (363 aa) |
| | secA | Preprotein translocase subunit SecA; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane. (797 aa) |
| | phnL-2 | ABC transporter, ATPase component; Paralog group: ATP_00013, ATP_00101, ATP_00485; Belongs to the ABC transporter superfamily. Macrolide exporter (TC 3.A.1.122) family. (544 aa) |
| | engB | Ribosome biogenesis GTP-binding protein; Necessary for normal cell division and for the maintenance of normal septation; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family. (196 aa) |
| | norM | Na+-driven multidrug efflux pump. (503 aa) |
| | tyrS | Tyrosyl-tRNA synthetase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. (417 aa) |
| | srmB-2 | Superfamily II DNA and RNA helicase. (531 aa) |
| | efp | Translation elongation factor P (EF-P); Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. (188 aa) |
| | rny | Putative phosphodiesterase; Endoribonuclease that initiates mRNA decay. Belongs to the RNase Y family. (525 aa) |
| | serS | Seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (423 aa) |
| | rpsT | 30S ribosomal protein S20; Binds directly to 16S ribosomal RNA. (87 aa) |
| | recA | RecA/RadA recombinase; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage; Belongs to the RecA family. (339 aa) |
| | gidA | Glucose-inhibited division protein A; NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34; Belongs to the MnmG family. (624 aa) |
| | rpmE | 50S ribosomal protein L31; Binds the 23S rRNA; Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily. (69 aa) |
| | ATP_00146 | AAA+ ATPase. (409 aa) |
| | tatD | Mg-dependent DNase. (250 aa) |
| | asnS | Asparaginyl-tRNA synthetase. (465 aa) |
| | thdF | tRNA modification GTPase; Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. (452 aa) |
| | thrS | Threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (640 aa) |
| | ruvB | Holliday junction resolvasome, helicase subunit; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. (332 aa) |
| | ruvA | Holliday junction resolvasome, DNA-binding subunit; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. (186 aa) |
| | prfA | Peptide chain release factor 1 (RF-1). (347 aa) |
| | dnaB1 | Replicative DNA helicase; Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. Belongs to the helicase family. DnaB subfamily. (450 aa) |
| | rplI | 50S ribosomal protein L9; Binds to the 23S rRNA. (837 aa) |
| | rpsR | 30S ribosomal protein S18; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (78 aa) |
| | ssb | Putative single-strand DNA-binding protein. (138 aa) |
| | rpsF | 30S ribosomal protein S6; Binds together with S18 to 16S ribosomal RNA. (94 aa) |
| | spoT | Guanosine-3'-5'-bis(diphosphate) 3'-pyrophosphohydrolase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. (734 aa) |
| | trmU | tRNA (5-methylaminomethyl-2-thioruidylate) methyltransferase; Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. (385 aa) |
| | qns | Glutamine-dependent NAD(+) synthetase. (598 aa) |
| | groES | Chaperonin 10kDa, small subunit; Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter; Belongs to the GroES chaperonin family. (87 aa) |
| | groEL | Chaperonin 60kDa, large subunit; Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. Belongs to the chaperonin (HSP60) family. (536 aa) |
| | dnaA | Chromosomal replication initiator protein; Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'- TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. (455 aa) |
| | dnaN | DNA polymerase sliding camp subunit; Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation [...] (376 aa) |
| | gyrB | DNA gyrase, beta subunit; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (633 aa) |
| | gyrA | DNA gyrase, alpha subunit; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (838 aa) |
| | glnS | glutaminyl-tRNA synthetase. (540 aa) |
| | hrcA | Negative regulator of class I heat shock protein; Negative regulator of class I heat shock genes (grpE-dnaK- dnaJ and groELS operons). Prevents heat-shock induction of these operons. (343 aa) |
| | grpE | Chaperone GrpE; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent [...] (235 aa) |
| | dnaK | Molecular chaperone; Acts as a chaperone; Belongs to the heat shock protein 70 family. (615 aa) |
| | dnaJ | Chaperone protein; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK a [...] (368 aa) |
| | recU | Holliday junction-specific endonuclease; Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation; Belongs to the RecU family. (190 aa) |
| | ATP_00210 | Zn-dependent hydrolase. (559 aa) |
| | ykqC | RNaSE J1/J2; An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay. (564 aa) |
| | spoU | tRNA/rRNA methyltransferase. (238 aa) |
| | pth | Peptidyl-tRNA hydrolase; The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Belongs to the PTH family. (176 aa) |
| | ksgA | Dimethyladenosine transferase; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (269 aa) |
| | rpsB | 30S ribosomal protein S2; Belongs to the universal ribosomal protein uS2 family. (244 aa) |
| | tsf | Elongation factor Ts (EF-Ts); Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Belongs to the EF-Ts family. (279 aa) |
| | pyrH | Uridylate kinase; Catalyzes the reversible phosphorylation of UMP to UDP. (241 aa) |
| | frr | Ribosome recycling factor. (185 aa) |
| | engD | GTP-dependent nucleic acid-binding protein; ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner. (365 aa) |
| | ileS | Isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (912 aa) |
| | ATP_00253 | Conserved hypothetical protein. (234 aa) |
| | holA | DNA polymerase III, delta subunit. (324 aa) |
| | ATP_00265 | Conserved hypothetical protein. (955 aa) |
| | rpsO | 30S ribosomal protein S15; Belongs to the universal ribosomal protein uS15 family. (71 aa) |
| | hisS | Histidyl-tRNA synthetase. (423 aa) |
| | aspS | Aspartyl-tRNA synthetase; Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp). Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (574 aa) |
| | tilS | tRNA(Ile)-lysidine synthase; Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. Belongs to the tRNA(Ile)-lysidine synthase family. (415 aa) |
| | hflB-2 | HflB protease; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins; In the central section; belongs to the AAA ATPase family. (686 aa) |
| | rluA | Pseudouridylate synthase; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (299 aa) |
| | lig | NAD-dependent DNA ligase; DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. (659 aa) |
| | ATP_00276 | Probable hemolysin. (428 aa) |
| | rpmB | 50S ribosomal protein L28; Belongs to the bacterial ribosomal protein bL28 family. (62 aa) |
| | obgE | GTPase ObgE; An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control. Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. (421 aa) |
| | rpmA | 50S ribosomal protein L27; Belongs to the bacterial ribosomal protein bL27 family. (96 aa) |
| | rplU | 50S ribosomal protein L21; This protein binds to 23S rRNA in the presence of protein L20; Belongs to the bacterial ribosomal protein bL21 family. (102 aa) |
| | lepA | GTP-binding protein LepA (elongation factor); Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner. (605 aa) |
| | ATP_00295 | Cold shock-like protein. (85 aa) |
| | metK | S-adenosylmethionine synthetase; Belongs to the AdoMet synthase family. (377 aa) |
| | tig | Trigger factor (TF); Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase; Belongs to the FKBP-type PPIase family. Tig subfamily. (437 aa) |
| | lonA | ATP-dependent proteinase La 1 (Lon); ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short- lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. (778 aa) |
| | ATP_00299 | Hypothetical protein. (79 aa) |
| | rpsU | 30S ribosomal protein S21; Belongs to the bacterial ribosomal protein bS21 family. (55 aa) |
| | era | Glycyl-tRNA synthetase; An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism. (293 aa) |
| | recO | Recombinational DNA repair protein O; Involved in DNA repair and RecF pathway recombination. (215 aa) |
| | glyQS | Glycyl-tRNA synthetase. (452 aa) |
| | dnaG1 | DNA primase; RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. (606 aa) |
| | rpoD | DNA-dependent RNA polymerase sigma subunits; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. (419 aa) |
| | rpmF | 50S ribosomal protein L32; Belongs to the bacterial ribosomal protein bL32 family. (61 aa) |
| | rpsD | SSU ribosomal protein S4; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. (199 aa) |
| | nusA | N utilization substance protein A; Participates in both transcription termination and antitermination. (358 aa) |
| | infB | Translation initiation factor IF-2; One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (620 aa) |
| | priA | Primosomal protein, replication factor Y; Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA; Belongs to the helicase family. PriA subfamily. (785 aa) |
| | truB | Pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (283 aa) |
| | smpB | SsrA-binding protein; Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to [...] (151 aa) |
| | mscL | Large-conductance mechanosensitive channel; Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell. (139 aa) |
| | rplM | 50S ribosomal protein L13; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (145 aa) |
| | rpsI | 30S ribosomal protein S9; Belongs to the universal ribosomal protein uS9 family. (129 aa) |
| | gltX | Glutamyl-tRNA synthetase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu); Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. (444 aa) |
| | cysS | Cysteinyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (438 aa) |
| | valS | Probable cation transport ATPase (similar to MgtA), fragment; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (863 aa) |
| | rpsJ | 30S ribosomal protein S10; Involved in the binding of tRNA to the ribosomes. Belongs to the universal ribosomal protein uS10 family. (104 aa) |
| | rplC | 50S ribosomal protein L3; One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit; Belongs to the universal ribosomal protein uL3 family. (219 aa) |
| | rplD | 50S ribosomal protein L4; One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. (207 aa) |
| | rplW | 50S ribosomal protein L23; One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome; Belongs to the universal ribosomal protein uL23 family. (94 aa) |
| | rplB | 50S ribosomal protein L2; One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Belongs to the universal ribosomal protein uL2 family. (280 aa) |
| | rpsS | 30S ribosomal protein S19; Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (93 aa) |
| | rplV | 50S ribosomal protein L22; This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (131 aa) |
| | rpsC | 30S ribosomal protein S3; Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation; Belongs to the universal ribosomal protein uS3 family. (245 aa) |
| | rplP | 50S ribosomal protein L16; Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs; Belongs to the universal ribosomal protein uL16 family. (140 aa) |
| | rpmC | 50S ribosomal protein L29; Belongs to the universal ribosomal protein uL29 family. (95 aa) |
| | rpsQ | 30S ribosomal protein S17; One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. (89 aa) |
| | rplN | 50S ribosomal protein L14; Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome; Belongs to the universal ribosomal protein uL14 family. (122 aa) |
| | rplX | 50S ribosomal protein L24; One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (115 aa) |
| | rplE | 50S ribosomal protein L5; This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. (178 aa) |
| | rpsN | 30S ribosomal protein S14. (89 aa) |
| | rpsH | 30S ribosomal protein S8; One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; Belongs to the universal ribosomal protein uS8 family. (130 aa) |
| | rplF | 50S ribosomal protein L6; This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center; Belongs to the universal ribosomal protein uL6 family. (181 aa) |
| | rplR | 50S ribosomal protein L18; This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. (117 aa) |
| | rpsE | 30S ribosomal protein S5; Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body. Belongs to the universal ribosomal protein uS5 family. (168 aa) |
| | rpmD | 50S ribosomal protein L30. (61 aa) |
| | rplO | 50S ribosomal protein L15; Binds to the 23S rRNA; Belongs to the universal ribosomal protein uL15 family. (146 aa) |
| | secY | Preprotein translocase subunit SecY; The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. (414 aa) |
| | map | Methionine aminopeptidase; Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed; Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. (248 aa) |
| | infA | Translation initiation factor IF-1; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (79 aa) |
| | rpmJ | 50S ribosomal protein L36; Belongs to the bacterial ribosomal protein bL36 family. (38 aa) |
| | rpsM | 30S ribosomal protein S13; Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. Belongs to the universal ribosomal protein uS13 family. (127 aa) |
| | rpsK | 30S ribosomal protein S11; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (130 aa) |
| | rpoA | DNA-directed RNA polymerase, alpha chain; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (331 aa) |
| | rplQ | 50S ribosomal protein L17. (118 aa) |
| | truA | Pseudouridine synthase A; Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. (244 aa) |
| | holB | DNA polymerase III, delta prime subunit. (318 aa) |
| | metG | Methiony-tRNA synthetase. (517 aa) |
| | rpmH | 50S ribosomal protein L34; Belongs to the bacterial ribosomal protein bL34 family. (44 aa) |
| | rnpA | Ribonuclease P; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (112 aa) |
| | yidC | Preprotein translocase subunit YidC. (572 aa) |
| | pnp | Polyribonucleotide nucleotidyltransferase; Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction. (728 aa) |
| | hflB-3 | HflB protease. (605 aa) |
| | rpmG | 50S ribosomal protein L33; Belongs to the bacterial ribosomal protein bL33 family. (49 aa) |
| | nusG | Transcription antitermination protein NusG; Participates in transcription elongation, termination and antitermination. (195 aa) |
| | rplK | 50S ribosomal protein L11; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. (141 aa) |
| | rplA | 50S ribosomal protein L1; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (231 aa) |
| | rplJ | 50S ribosomal protein L10; Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. Belongs to the universal ribosomal protein uL10 family. (162 aa) |
| | rplL | 50S ribosomal protein L7/L12; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family. (127 aa) |
| | rpoB | DNA-directed RNA polymerase, beta chain; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1273 aa) |
| | rpoC | DNA-directed RNA polymerase beta' chain; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1352 aa) |
| | rpsL | 30S ribosomal protein S12; With S4 and S5 plays an important role in translational accuracy. (137 aa) |
| | rpsG | 30S ribosomal protein S7; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | fusA | Elongation factor G 1 (EF-G 1); Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 su [...] (688 aa) |
| | tuf | Elongation factor Tu; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (392 aa) |
| | ssb-2 | Single-stranded DNA binding protein. (102 aa) |
| | dnaG2 | Conserved hypothetical protein, fragment; Paralog group: ATP_00263, ATP_00414. (390 aa) |
| | ATP_00420 | Probable replicative DNA helicase. (483 aa) |
| | polC2 | DNA polymerase III, alpha subunit; Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity. (1449 aa) |
| | pepA | Putative aminopeptidase; Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N- terminal amino acids from various peptides. (498 aa) |
| | uvrD | Superfamily I DNA and RNA helicases. (708 aa) |
| | proS | Prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). (473 aa) |
| | argS | Arginyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (565 aa) |
| | rpsP | 30S ribosomal protein S16; Belongs to the bacterial ribosomal protein bS16 family. (81 aa) |
| | rimM | 16S rRNA-processing protein; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (158 aa) |
| | trmD | tRNA (guanine-N(1)-)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (243 aa) |
| | rplS | 50S ribosomal protein L19; This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. (114 aa) |
| | infC | Translation initiation factor IF-3; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (185 aa) |
| | rpmI | 50S ribosomal protein L35; Belongs to the bacterial ribosomal protein bL35 family. (65 aa) |
| | rplT | LSU ribosomal protein L20; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit. (226 aa) |
| | leuS | Leucyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family. (811 aa) |
| | gmk | Guanylate kinase; Essential for recycling GMP and indirectly, cGMP. (210 aa) |
| | rpoZ | DNA-directed RNA polymerase, omega chain; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (71 aa) |
| | ATP_00459 | Predicted outer surface lipoprotein. (732 aa) |
| | ATP_00460 | AAA+ ATPase; Paralog group: ATP_00011, ATP_00406, ATP_00460, ATP_00487. (357 aa) |
| | nusB-2 | N utilization substance protein, transcription termination factor; Paralog group: ATP_00029, ATP_00469. (142 aa) |
| | srmB-3 | Superfamily II DNA and RNA helicase; Paralog group: ATP_00028, ATP_00470. (398 aa) |
| | nfo-2 | Endonuclease IV; Paralog group: ATP_00027, ATP_00471. (286 aa) |
| | phnL-3 | ABC transporter, ATPase component; Paralog group: ATP_00013, ATP_00101, ATP_00485. (549 aa) |
