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| | gyrB | DNA gyrase subunit B; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (824 aa) |
| | rpmH | Large subunit ribosomal protein L34; KEGG, K02914; Belongs to the bacterial ribosomal protein bL34 family. (44 aa) |
| | rnpA | Ribonuclease P; RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. (135 aa) |
| | BAG42053.1 | Conserved hypothetical protein; Could be involved in insertion of integral membrane proteins into the membrane; Belongs to the UPF0161 family. (88 aa) |
| | yidC | Preprotein translocase YidC subunit; Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins. (553 aa) |
| | thdF | tRNA modification GTPase; Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. (464 aa) |
| | dksA | DnaK suppressor protein; Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters. (138 aa) |
| | hemK | HemK protein; Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif; Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily. (280 aa) |
| | prfA | Peptide chain release factor RF-1; Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. (360 aa) |
| | BAG42327.1 | COG0488: ATPase components of ABC transporters with duplicated ATPase domains, Ralstonia solanacearum; KEGG, K06020. (555 aa) |
| | ychF | Probable translation factor; ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner. (364 aa) |
| | secA | Preprotein translocase SecA subunit; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane. Belongs to the SecA family. (932 aa) |
| | yacG | Conserved hypothetical protein; Inhibits all the catalytic activities of DNA gyrase by preventing its interaction with DNA. Acts by binding directly to the C- terminal domain of GyrB, which probably disrupts DNA binding by the gyrase. (65 aa) |
| | rplU | Large subunit ribosomal protein L21; This protein binds to 23S rRNA in the presence of protein L20; Belongs to the bacterial ribosomal protein bL21 family. (103 aa) |
| | rpmA | COG0211: Ribosomal protein L27, Ralstonia solanacearum; KEGG, K02899; Belongs to the bacterial ribosomal protein bL27 family. (87 aa) |
| | obg | GTP-binding protein; An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control. Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. (369 aa) |
| | proS | prolyl-tRNA synthetase; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves dea [...] (578 aa) |
| | ffh | Signal recognition particle subunit SRP54; Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual co [...] (455 aa) |
| | prmA | Ribosomal protein L11 methyltransferase; Methylates ribosomal protein L11; Belongs to the methyltransferase superfamily. PrmA family. (300 aa) |
| | BAG42453.1 | Conserved hypothetical protein; Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit. (254 aa) |
| | leuS | COG0495: Leucyl-tRNA synthetase, Ralstonia solanacearum; KEGG, K01869; Belongs to the class-I aminoacyl-tRNA synthetase family. (864 aa) |
| | rplM | Large subunit ribosomal protein L13; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. (142 aa) |
| | rpsI | COG0103: Ribosomal protein S9, Ralstonia solanacearum; KEGG, K02996; Belongs to the universal ribosomal protein uS9 family. (130 aa) |
| | tyrS | tyrosyl-tRNA synthetase; Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr); Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. (413 aa) |
| | secF | Preprotein translocase SecF subunit; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. (316 aa) |
| | secD | Preprotein translocase SecD subunit; Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. (673 aa) |
| | yajC | Preprotein translocase YajC subunit; The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein secretase/insertase is a supercomplex required for protein secretion, insertion of proteins into membranes, and assembly of membrane protein complexes. While the SecYEG complex is essential for assembly of a number of proteins and complexes, the SecDF-YajC-YidC subcomplex facilitates these functions. (107 aa) |
| | nusB | N utilization substance protein B; Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons. (145 aa) |
| | serS | seryl-tRNA synthetase; Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec). (433 aa) |
| | rpoZ | DNA-directed RNA polymerase subunit omega; Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. (67 aa) |
| | relA | GTP pyrophosphokinase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. (788 aa) |
| | greB | Transcription elongation factor; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length. (187 aa) |
| | gyrA | DNA gyrase subunit A; A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. (867 aa) |
| | rpsA | Small subunit ribosomal protein S1; Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence. (570 aa) |
| | rpsP | COG0228: Ribosomal protein S16, Ralstonia solanacearum; KEGG, K02959; Belongs to the bacterial ribosomal protein bS16 family. (84 aa) |
| | rimM | 16S rRNA processing protein; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes; Belongs to the RimM family. (223 aa) |
| | trmD | tRNA (guanine-N1-)-methyltransferase; Specifically methylates guanosine-37 in various tRNAs. Belongs to the RNA methyltransferase TrmD family. (264 aa) |
| | rplS | Large subunit ribosomal protein L19; This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. (130 aa) |
| | BAG42962.1 | COG3028: Uncharacterized protein conserved in bacteria, Ralstonia solanacearum; Belongs to the UPF0307 family. (203 aa) |
| | rluC | Ribosomal large subunit pseudouridine synthase C; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (334 aa) |
| | BAG42999.1 | Conserved hypothetical protein; COG1399: Predicted metal-binding, possibly nucleic acid-binding protein, Ralstonia solanacearum; KEGG, K07040. (211 aa) |
| | rpmF | COG0333: Ribosomal protein L32, Ralstonia solanacearum; KEGG, K02911; Belongs to the bacterial ribosomal protein bL32 family. (59 aa) |
| | lepA | GTP-binding protein; Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner. (597 aa) |
| | rnc | Ribonuclease III; Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. (406 aa) |
| | era | GTP-binding protein; An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism. (299 aa) |
| | efp | Elongation factor EF-P; Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. (185 aa) |
| | BAG43023.1 | COG4394: Uncharacterized protein conserved in bacteria, Ralstonia solanacearum. (397 aa) |
| | map | Methionyl aminopeptidase; Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed; Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. (268 aa) |
| | BAG43098.1 | Putative peptidyl-tRNA hydrolase; COG1186: Protein chain release factor B, Pseudomonas aeruginosa. (134 aa) |
| | ftsH-2 | Cell division protease; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins; Belongs to the AAA ATPase family. In the central section; belongs to the AAA ATPase family. (631 aa) |
| | ppx | COG0248: Exopolyphosphatase, Ralstonia solanacearum; KEGG, K01514. (504 aa) |
| | valS | valyl-tRNA synthetase; Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner; Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. (955 aa) |
| | relA-2 | GTP pyrophosphokinase; In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. (744 aa) |
| | thrS | threonyl-tRNA synthetase; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr). (635 aa) |
| | infC | Translation initiation factor IF-3; IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. (156 aa) |
| | rpmI | COG0291: Ribosomal protein L35, Ralstonia solanacearum; KEGG, K02916; Belongs to the bacterial ribosomal protein bL35 family. (65 aa) |
| | rplT | Large subunit ribosomal protein L20; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit. (119 aa) |
| | pheS | COG0016: Phenylalanyl-tRNA synthetase alpha subunit, Ralstonia solanacearum; KEGG, K01888. (337 aa) |
| | pheT | COG0072: Phenylalanyl-tRNA synthetase beta subunit, Ralstonia solanacearum; KEGG, K06878. (809 aa) |
| | rimP | Conserved hypothetical protein; Required for maturation of 30S ribosomal subunits. Belongs to the RimP family. (152 aa) |
| | nusA | N utilization substance protein A; Participates in both transcription termination and antitermination. (491 aa) |
| | infB | Translation initiation factor IF-2; One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. (969 aa) |
| | rbfA | Ribosome-binding factor A; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA. (132 aa) |
| | truB | tRNA pseudouridine synthase B; Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs; Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. (310 aa) |
| | typA | GTP-binding protein; COG1217: Predicted membrane GTPase involved in stress response, Ralstonia solanacearum; KEGG, K06207. (608 aa) |
| | yjfH | TrmH family RNA methyltransferase; Specifically methylates the ribose of guanosine 2251 in 23S rRNA. (247 aa) |
| | BAG43662.1 | COG0488: ATPase components of ABC transporters with duplicated ATPase domains, Ralstonia solanacearum. (530 aa) |
| | rluD | Ribosomal large subunit pseudouridine synthase D; Responsible for synthesis of pseudouridine from uracil. Belongs to the pseudouridine synthase RluA family. (400 aa) |
| | hisS | COG0124: Histidyl-tRNA synthetase, Ralstonia solanacearum; KEGG, K01891. (446 aa) |
| | ndk | Nucleoside-diphosphate kinase; Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate; Belongs to the NDK family. (141 aa) |
| | rho | Transcription termination factor; Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. (420 aa) |
| | rpmE | COG0254: Ribosomal protein L31, Ralstonia solanacearum; KEGG, K02909. (87 aa) |
| | rplI | Large subunit ribosomal protein L9; Binds to the 23S rRNA. (150 aa) |
| | rpsR | Small subunit ribosomal protein S18; Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit; Belongs to the bacterial ribosomal protein bS18 family. (91 aa) |
| | priB | Primosomal replication protein N; Binds single-stranded DNA at the primosome assembly site (PAS); Belongs to the PriB family. (99 aa) |
| | rpsF | Small subunit ribosomal protein S6; Binds together with S18 to 16S ribosomal RNA. (124 aa) |
| | tig | Trigger factor; Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase; Belongs to the FKBP-type PPIase family. Tig subfamily. (448 aa) |
| | smpB | SsrA-binding protein; Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to [...] (148 aa) |
| | spoU | TrmH family RNA methyltransferase; COG0566: rRNA methylases, Ralstonia solanacearum; KEGG, K03437. (260 aa) |
| | frr | Ribosome recycling factor; Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another; Belongs to the RRF family. (186 aa) |
| | tsf | Elongation factor EF-Ts; Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Belongs to the EF-Ts family. (293 aa) |
| | rpsB | COG0052: Ribosomal protein S2, Ralstonia solanacearum; KEGG, K02967; Belongs to the universal ribosomal protein uS2 family. (246 aa) |
| | map-2 | Methionyl aminopeptidase; Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed; Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily. (271 aa) |
| | rluF | Ribosomal large subunit pseudouridine synthase F; COG1187: 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases, Ralstonia solanacearum; KEGG, K06182; Belongs to the pseudouridine synthase RsuA family. (612 aa) |
| | def-2 | Polypeptide deformylase; Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. (177 aa) |
| | ligA | NAD-dependent DNA ligase; DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. (691 aa) |
| | yfcB | Putative adenine-specific DNA-methyltransferase; Specifically methylates the 50S ribosomal protein L3 on a specific glutamine residue; Belongs to the protein N5-glutamine methyltransferase family. PrmB subfamily. (302 aa) |
| | BAG43923.1 | ATP-binding cassette; COG0488: ATPase components of ABC transporters with duplicated ATPase domains, Ralstonia solanacearum; KEGG, K06158. (643 aa) |
| | deaD | COG0513: Superfamily II DNA and RNA helicases, Ralstonia solanacearum; DeaD protein; KEGG, K05592; Belongs to the DEAD box helicase family. (505 aa) |
| | gltX | glutamyl-tRNA synthetase; Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu); Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. (469 aa) |
| | BAG43963.1 | Peptidylprolyl isomerase; PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides; Belongs to the cyclophilin-type PPIase family. (191 aa) |
| | impA | Myo-inositol-1(or 4)-monophosphatase; COG0483: Archaeal fructose-1,6-bisphosphatase and related enzymes of inositol monophosphatase family, Ralstonia solanacearum; KEGG, K01092. (267 aa) |
| | prfB | Peptide chain release factor 2; Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. (248 aa) |
| | lysU | COG1190: Lysyl-tRNA synthetase (class II), Ralstonia solanacearum; KEGG, K04567; Belongs to the class-II aminoacyl-tRNA synthetase family. (508 aa) |
| | secG | Preprotein translocase SecG subunit; Involved in protein export. Participates in an early event of protein translocation; Belongs to the SecG family. (125 aa) |
| | rpsO | Small subunit ribosomal protein S15; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA. (89 aa) |
| | BAG44175.1 | COG0217: Uncharacterized conserved protein, Ralstonia solanacearum. (242 aa) |
| | rsfS | Iojap domain protein; Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation. (149 aa) |
| | ybeA | Conserved hypothetical protein; Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA; Belongs to the RNA methyltransferase RlmH family. (156 aa) |
| | gltX-2 | glutamyl-tRNA synthetase; Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon; Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily. (298 aa) |
| | parC | Topoisomerase IV subunit A; Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule; Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 1 subfamily. (773 aa) |
| | parE | Topoisomerase IV subunit B; Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule; Belongs to the type II topoisomerase family. ParE type 1 subfamily. (660 aa) |
| | BAG44241.1 | COG0488: ATPase components of ABC transporters with duplicated ATPase domains, Ralstonia solanacearum; KEGG, K06020. (649 aa) |
| | BAG44250.1 | Conserved hypothetical protein. (114 aa) |
| | ppa | Inorganic pyrophosphatase; Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions. (175 aa) |
| | metG | methionyl-tRNA synthetase; Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. (722 aa) |
| | rpmB | COG0227: Ribosomal protein L28, Ralstonia solanacearum; KEGG, K02902; Belongs to the bacterial ribosomal protein bL28 family. (77 aa) |
| | rpmG | COG0267: Ribosomal protein L33, Ralstonia solanacearum; KEGG, K02913; Belongs to the bacterial ribosomal protein bL33 family. (55 aa) |
| | ileS | isoleucyl-tRNA synthetase; Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. (945 aa) |
| | fusA | Elongation factor EF-G; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. (701 aa) |
| | sod | Superoxide dismutase; Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Belongs to the iron/manganese superoxide dismutase family. (192 aa) |
| | rpsT | Small subunit ribosomal protein S20; Binds directly to 16S ribosomal RNA. (90 aa) |
| | glyQ | COG0752: Glycyl-tRNA synthetase, alpha subunit, Ralstonia solanacearum; KEGG, K01878. (334 aa) |
| | glyS | COG0751: Glycyl-tRNA synthetase, beta subunit, Ralstonia solanacearum; KEGG, K01879. (699 aa) |
| | ksgA | Dimethyladenosine transferase; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. (275 aa) |
| | aspS | aspartyl-tRNA synthetase; Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn); Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily. (600 aa) |
| | yhbH | Putative sigma54 modulation protein; COG1544: Ribosome-associated protein Y (PSrp-1), Ralstonia solanacearum; KEGG, K05808. (119 aa) |
| | rplY | Large subunit ribosomal protein L25; This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance. Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily. (201 aa) |
| | pth1 | PTH1 family peptidyl-tRNA hydrolase; The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Belongs to the PTH family. (199 aa) |
| | BAG44650.1 | COG0742: N6-adenine-specific methylase, Ralstonia solanacearum; KEGG, K00599. (204 aa) |
| | ftsY | Signal recognition particle receptor; Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components. (380 aa) |
| | rpoH | RNA polymerase sigma-32 factor; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. (311 aa) |
| | cspR | TrmH family RNA methyltransferase; Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. (156 aa) |
| | secB | Preprotein translocase SecB subunit; One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. (160 aa) |
| | argS | COG0018: Arginyl-tRNA synthetase, Ralstonia solanacearum; KEGG, K01887. (598 aa) |
| | engB | GTP-binding protein; Necessary for normal cell division and for the maintenance of normal septation; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family. (219 aa) |
| | rplQ | COG0203: Ribosomal protein L17, Ralstonia solanacearum; KEGG, K02879. (131 aa) |
| | rpoA | DNA-directed RNA polymerase subunit alpha; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (325 aa) |
| | rpsD | Small subunit ribosomal protein S4; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. (207 aa) |
| | rpsK | Small subunit ribosomal protein S11; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine- Dalgarno cleft in the 70S ribosome; Belongs to the universal ribosomal protein uS11 family. (133 aa) |
| | rpsM | Small subunit ribosomal protein S13; Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. Belongs to the universal ribosomal protein uS13 family. (121 aa) |
| | rpmJ | Large subunit ribosomal protein L36; KEGG, K02919; Belongs to the bacterial ribosomal protein bL36 family. (38 aa) |
| | infA | Translation initiation factor IF-1; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (72 aa) |
| | secY | Preprotein translocase SecY subunit; The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. (449 aa) |
| | rplO | Large subunit ribosomal protein L15; Binds to the 23S rRNA; Belongs to the universal ribosomal protein uL15 family. (144 aa) |
| | rpmD | COG1841: Ribosomal protein L30/L7E, Ralstonia solanacearum; KEGG, K02907. (60 aa) |
| | rpsE | Small subunit ribosomal protein S5; With S4 and S12 plays an important role in translational accuracy; Belongs to the universal ribosomal protein uS5 family. (172 aa) |
| | rplR | Large subunit ribosomal protein L18; This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. (121 aa) |
| | rplF | Large subunit ribosomal protein L6; This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center; Belongs to the universal ribosomal protein uL6 family. (176 aa) |
| | rpsH | Small subunit ribosomal protein S8; One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; Belongs to the universal ribosomal protein uS8 family. (131 aa) |
| | rpsN | Small subunit ribosomal protein S14; Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site; Belongs to the universal ribosomal protein uS14 family. (101 aa) |
| | rplE | Large subunit ribosomal protein L5; This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. (179 aa) |
| | rplX | Large subunit ribosomal protein L24; One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (102 aa) |
| | rplN | Large subunit ribosomal protein L14; Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome; Belongs to the universal ribosomal protein uL14 family. (122 aa) |
| | rpsQ | Small subunit ribosomal protein S17; One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. (90 aa) |
| | rpmC | COG0255: Ribosomal protein L29, Ralstonia solanacearum; KEGG, K02904; Belongs to the universal ribosomal protein uL29 family. (64 aa) |
| | rplP | Large subunit ribosomal protein L16; Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs; Belongs to the universal ribosomal protein uL16 family. (138 aa) |
| | rpsC | Small subunit ribosomal protein S3; Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation; Belongs to the universal ribosomal protein uS3 family. (266 aa) |
| | rplV | Large subunit ribosomal protein L22; This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (109 aa) |
| | rpsS | Small subunit ribosomal protein S19; Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. (91 aa) |
| | rplB | Large subunit ribosomal protein L2; One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Belongs to the universal ribosomal protein uL2 family. (275 aa) |
| | rplW | Large subunit ribosomal protein L23; One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome; Belongs to the universal ribosomal protein uL23 family. (104 aa) |
| | rplD | Large subunit ribosomal protein L4; One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. (206 aa) |
| | rplC | Large subunit ribosomal protein L3; One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. (216 aa) |
| | rpsJ | Small subunit ribosomal protein S10; Involved in the binding of tRNA to the ribosomes. Belongs to the universal ribosomal protein uS10 family. (103 aa) |
| | tufA | Elongation factor EF-Tu; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa) |
| | fusA-2 | Elongation factor EF-G; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. (700 aa) |
| | rpsG | Small subunit ribosomal protein S7; One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA; Belongs to the universal ribosomal protein uS7 family. (156 aa) |
| | rpsL | Small subunit ribosomal protein S12; With S4 and S5 plays an important role in translational accuracy. (126 aa) |
| | rpoC | DNA-directed RNA polymerase, beta' subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1413 aa) |
| | rpoB | DNA-directed RNA polymerase, beta subunit; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (1368 aa) |
| | rplL | Large subunit ribosomal protein L7/L12; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation; Belongs to the bacterial ribosomal protein bL12 family. (124 aa) |
| | rplJ | Large subunit ribosomal protein L10; Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. Belongs to the universal ribosomal protein uL10 family. (165 aa) |
| | rplA | Large subunit ribosomal protein L1; Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release. (232 aa) |
| | rplK | Large subunit ribosomal protein L11; Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. (143 aa) |
| | nusG | Transcriptional antiterminator; Participates in transcription elongation, termination and antitermination. (185 aa) |
| | secE | Preprotein translocase SecE subunit; Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation; Belongs to the SecE/SEC61-gamma family. (126 aa) |
| | tufA-2 | Elongation factor EF-Tu; This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. (396 aa) |
| | BAG44921.1 | COG1881: Phospholipid-binding protein, Salmonella typhimurium LT2; KEGG, K06910. (196 aa) |
| | rsmI | Methyltransferase; Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. (292 aa) |
| | rpsU | COG0828: Ribosomal protein S21, Ralstonia solanacearum; KEGG, K02970; Belongs to the bacterial ribosomal protein bS21 family. (70 aa) |
| | atpF1E | F-type H+-transporting ATPase epsilon chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. (141 aa) |
| | atpF1B | F-type H+-transporting ATPase beta chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits; Belongs to the ATPase alpha/beta chains family. (464 aa) |
| | atpF1G | F-type H+-transporting ATPase gamma chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. (291 aa) |
| | atpF1A | F-type H+-transporting ATPase alpha chain; Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit. Belongs to the ATPase alpha/beta chains family. (513 aa) |
| | atpF1D | F-type H+-transporting ATPase delta chain; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation; Belongs to the ATPase delta chain family. (179 aa) |
| | atpF0B | F-type H+-transporting ATPase b chain; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. (156 aa) |
| | atpF0C | F-type H+-transporting ATPase c chain; F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. (89 aa) |
| | atpF0A | F-type H+-transporting ATPase a chain; Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane. (283 aa) |
| | atpI | ATP synthase protein I; KEGG, K02116. (174 aa) |
| | gidB | Glucose inhibited division protein B; Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. (228 aa) |
| | gidA | Glucose inhibited division protein A; NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34; Belongs to the MnmG family. (656 aa) |
| | BAG45565.1 | COG4638: Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit, Ralstonia solanacearum; KEGG, K00499. (368 aa) |
| | rpsU-2 | COG0828: Ribosomal protein S21, Neisseria meningitidis Z2491; KEGG, K02970; Belongs to the bacterial ribosomal protein bS21 family. (70 aa) |
| | rpoD | RNA polymerase primary sigma factor; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. (804 aa) |
| | BMULJ_03747 | Uncharacterized protein; OrfB, inactivated by ISBmu4 insertion, C-terminal portion; inactivated ISBmu28 transposase OrfB. (117 aa) |
| | BAG45662.1 | Hypothetical protein. (222 aa) |
| | selB | COG3276: Selenocysteine-specific translation elongation factor, Pseudomonas aeruginosa; KEGG, K03833. (641 aa) |
| | rpoS-2 | RNA polymerase sigma 38 subunit; Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. (525 aa) |
| | infA-2 | Translation initiation factor IF-1; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. (88 aa) |
| | repA3 | RepA3 protein. (454 aa) |
| | BAG47404.1 | Hypothetical protein. (398 aa) |
| | BAG47474.1 | Conserved hypothetical protein. (169 aa) |
| | rfbB-3 | dTDP-glucose 4,6-dehydratase; COG0451: Nucleoside-diphosphate-sugar epimerases, Xylella fastidiosa 9a5c; KEGG, K01710. (348 aa) |
| | rpsU-3 | COG0828: Ribosomal protein S21, Ralstonia solanacearum; KEGG, K02970; Belongs to the bacterial ribosomal protein bS21 family. (70 aa) |
| | spoT | Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase; KEGG, K01139. (174 aa) |
