Putative regulator AldR; Putative translation initiation inhibitor, PF01042: Endoribonuclease L-PSP, TIGR00004: endoribonuclease L-PSP, putative; Conserved hypothetical protein.

IlvA protein; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.

Putative regulator AldR; Putative translation initiation inhibitor, PF01042: Endoribonuclease L-PSP, TIGR00004: endoribonuclease L-PSP, putative; Conserved hypothetical protein.

IlvC protein; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.

Ammonium transporter AmtB; High confidence in function and specificity.

Aconitate hydratase; Catalyzes the isomerization of citrate to isocitrate via cis- aconitate.

Ammonium transporter AmtB; High confidence in function and specificity.

GlnA protein; PF00120: Glutamine synthetase, catalytic domain, TIGR00653: glutamine synthetase, type I; High confidence in function and specificity.

Ammonium transporter AmtB; High confidence in function and specificity.

Nitrogen regulatory protein P-II; High confidence in function and specificity; Belongs to the P(II) protein family.

Ammonium transporter AmtB; High confidence in function and specificity.

GltA protein; cit_synth_II: 2-methylcitrate synthase/citrate synthase II; High confidence in function and specificity; Belongs to the citrate synthase family.

Ammonium transporter AmtB; High confidence in function and specificity.

Glutamate synthase, large subunit; PF01645: Conserved region in glutamate synthase; High confidence in function and specificity.

Ammonium transporter AmtB; High confidence in function and specificity.

TIGR01317: glutamate synthases, NADH/NADPH, small subunit; High confidence in function and specificity.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

Dihydrodipicolinate synthase; Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

DapB protein; Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase; Catalyzes the transfer of an acetyl group from acetyl-CoA to tetrahydrodipicolinate.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

Glutamate synthase, large subunit; PF01645: Conserved region in glutamate synthase; High confidence in function and specificity.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

Hom protein; High confidence in function and specificity.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

Isocitrate dehydrogenase; High confidence in function and specificity.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

PF00920: Dehydratase family, TIGR00110: dihydroxy-acid dehydratase; High confidence in function and specificity; Belongs to the IlvD/Edd family.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

PF01063: Aminotransferase class IV, TIGR01123: branched-chain amino acid aminotransferase; High confidence in function and specificity.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

IPR004789: Acetolactate synthase, small subunit, TIGR00119: acetolactate synthase, small subunit; High confidence in function and specificity.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

LeuC protein; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

3-isopropylmalate dehydratase, small subunit; Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate. Belongs to the LeuD family. LeuD type 1 subfamily.

Asd protein; Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate; Belongs to the aspartate-semialdehyde dehydrogenase family.

LysA protein; PF00278: Pyridoxal-dependent decarboxylase, C-terminal sheet domain, TIGR01048: diaminopimelate decarboxylase; High confidence in function and specificity.