Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Nuclear fusion protein BIK1; Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Protein BIM1; Microtubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally; homolog of human end binding protein 1 (EB1); Belongs to the MAPRE family.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Protein BNI1; Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; recruited to the division site in a Glc7p/Ref2p dependent manner following release of Bnr1p; functionally redundant with BNR1.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

BNI1-related protein 1; Formin; nucleates the formation of linear actin filaments; involved in processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; activity is regulated by Hof1p and by the Bud14p-Kel1p-Kel2p complex; dephosphorylated and delocalized from the division site in a Glc7p/Ref2p-dependent manner; functionally redundant with BNI1.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Mitotic check point protein BUB2; Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage; Belongs to the BUB2 family.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Cell division control protein 12; Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Tyrosine-protein phosphatase CDC14; Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, environmental stress response; held in nucleolus by Cdc55p in early meiosis, liberated by FEAR and Mitotic Exit Network in anaphase, enabling it to effect a decrease in CDK/B-cyclin activity and mitotic exit; sequestered in metaphase II, released upon entry into anaphase II; human homolog CDC14A can complement thermosensitivity of yeast cdc14-1 mutant.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Cell division control protein 15; Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Cell division control protein 24; Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Cell division control protein 42; Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; mutants have defects in the organization of actin and septins; human homolog CDC42 can complement yeast cdc42 mutant.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Serine/threonine-protein kinase CLA4; Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

G2/mitotic-specific cyclin-2; B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Protein DSS4; Guanine nucleotide dissociation stimulator for Sec4p; functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol; Belongs to the DSS4/MSS4 family.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Kinesin-like protein KAR3; Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Karyogamy protein KAR9; Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Kinesin-like protein KIP2; Kinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; functions as a microtubule polymerase and catastrophe inhibitor in vitro; Kip2p levels are controlled during the cell cycle.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Guanine nucleotide exchange factor LTE1; Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex.

Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.

Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane; Belongs to the SPC42 family.