Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

DnaK-like molecular chaperone specific for IscU; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU; Belongs to the heat shock protein 70 family.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

HscA co-chaperone, J domain-containing protein Hsc56; Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA; Belongs to the HscB family.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

Isc operon transcriptional repressor; Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

Cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent; Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Preferentially binds to disordered IscU on which the Fe-S is assembled, IscU converts to the structured state and then dissociates from IscS to transfer the Fe-S to a [...]

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

Iron-sulfur cluster assembly scaffold protein; A scaffold on which IscS assembles Fe-S clusters. Exists as 2 interconverting forms, a structured (S) and disordered (D) form. The D- state is the preferred substrate for IscS. Converts to the S-state when an Fe-S cluster is assembled, which helps it dissociate from IscS to transfer the Fe-S to an acceptor. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters; Belongs to the NifU family.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

Fe(2+) donor and activity modulator for cysteine desulfurase; May function as iron donor in the assembly of iron-sulfur clusters; Belongs to the IscX family.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

Uncharacterized ferredoxin-like protein YfaE; Ferredoxin involved with ribonucleotide reductase diferric-tyrosyl radical (Y*) cofactor maintenance.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

DnaK-like molecular chaperone specific for IscU; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU; Belongs to the heat shock protein 70 family.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

HscA co-chaperone, J domain-containing protein Hsc56; Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA; Belongs to the HscB family.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

Isc operon transcriptional repressor; Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

Cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent; Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Preferentially binds to disordered IscU on which the Fe-S is assembled, IscU converts to the structured state and then dissociates from IscS to transfer the Fe-S to a [...]

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

Iron-sulfur cluster assembly scaffold protein; A scaffold on which IscS assembles Fe-S clusters. Exists as 2 interconverting forms, a structured (S) and disordered (D) form. The D- state is the preferred substrate for IscS. Converts to the S-state when an Fe-S cluster is assembled, which helps it dissociate from IscS to transfer the Fe-S to an acceptor. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters; Belongs to the NifU family.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

Fe(2+) donor and activity modulator for cysteine desulfurase; May function as iron donor in the assembly of iron-sulfur clusters; Belongs to the IscX family.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

Uncharacterized ferredoxin-like protein YfaE; Ferredoxin involved with ribonucleotide reductase diferric-tyrosyl radical (Y*) cofactor maintenance.

DnaK-like molecular chaperone specific for IscU; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU; Belongs to the heat shock protein 70 family.

Iron-dependent inhibitor of iron-sulfur cluster formation; Involved in iron-sulfur (Fe-S) cluster assembly. May act as a regulator of Fe-S biogenesis. Can bind both Fe(2+) and Fe(3+) ions. In vivo, has a positive effect on Fe-S cluster biogenesis under iron- rich growth conditions. In vitro, can inhibit IscS cysteine desulfurase activity and the formation of Fe-S clusters on IscU. In vitro, in the presence of IscS and cysteine, Fe(3+)-CyaY can be used as an iron donor during Fe-S cluster assembly on the scaffold protein IscU.

DnaK-like molecular chaperone specific for IscU; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU; Belongs to the heat shock protein 70 family.

[2Fe-2S] ferredoxin; Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron-sulfur proteins.

DnaK-like molecular chaperone specific for IscU; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU; Belongs to the heat shock protein 70 family.

HscA co-chaperone, J domain-containing protein Hsc56; Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA; Belongs to the HscB family.

DnaK-like molecular chaperone specific for IscU; Chaperone involved in the maturation of iron-sulfur cluster- containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU; Belongs to the heat shock protein 70 family.

Isc operon transcriptional repressor; Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA.